|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | Dictation as a technique for teaching composition显示文摘 | Ong K A | | 0，，： | 2 |
| 3 | Resistance of fibre concrete slabs to low velocity projectile impact显示文摘 | Ong K C Baxkeerkhan M Paramasivam P | 1999 | Cement & Concrete Composites1999，21，4： | 2 |
| 4 | Interoperability of Design Intent in Integrated Product and Process Design显示文摘Concurrent engineering is currently an overwhelming t rend in product development since it takes the down-stream product development issues and product life-cycle issues into consideration in up-front design pro cess. At this stage, evaluation, verification and validation of design concepts and design schemes is critical to the right-to-market product development. I dentification and extraction of design intent from the part digital mock-ups in different data formats and created in different CAD systems for DFX considerati on, evaluation, verification, and validation is crucial too. Traditionally, fro m manufacturing point of view, feature identification and extraction is to get t he features and feature-related information for manufacturing planning and inte gration. Presently, from the viewpoint of product development and product servi ce life cycle, design intent identification and extraction is to get all the des ign-related information for DFX consideration in early design stage, such as de sign for assembly, design for service, design for tooling, design for functional ity and design for environment etc. Therefore, how to successfully extract desi gn intent such as design and machining features and design-related information from the prototyping digital mock-ups created by different CAD systems for DFX consideration in upstream design stage is a very useful topic in integrated prod uct and process design and development. Presently, there are heterogeneous CAD systems in the market and the interoperat ion among the different CAD systems is indispensable. The interoperability of d ifferent CAD systems is by way of data exchanged technology such as STEP and IGE S. The critical weakness of this interoperation technology is that much of the design intent is lost in the interoperation process. Once the product digital m ock-ups is exchanged via data exchange technology such as IGES or STEP, the des ign intent including the dimension-driven design features and other attributes embedded in the part model are lost in the data translation process. Models rea d out of CAD systems come into other systems as featureless chunks of geometry e ntities. Obviously, the interoperation technology does not satisfy the industry need. It has been reported that this poor interoperation technology, which pro vides poor CAD models in neutral-format geometry had cost the automobile indust ry alone $1 billon per year. Fortunately, some researchers have been aware of t he criticality of this since only the United States industry spends billons of d ollars as a result of poor interoperability between computer-aided engineering software tools. In the past, product development was often done within a single company by co-located design team. In more recent years, there has been a busi ness paradigm shift in product development as the product development is being d one more often by geographically and temporally distributed design teams. There is a high level of outsourcing, not only of manufacturing but also of actual pr oduct development effort. Besides, product development across companies, and ev en within a single company, is often done within a heterogeneous software tool e nvironment. How to improve the interoperability of design intent created in het erogeneous software tool environment geographically has become a critical issue in next-generation product development system. Design intent in this research is defined as the outcome of design process. Des ign process goes from the identification of needs to satisfaction of that needs. Design intent satisfies this kind of needs. In this research, design intent i s created in a CAD system and embedded in part CAD model. The detailed design i ntent here includes design and manufacturing features and the feature-relat ed information such as feature parameters, feature relationships and some relate d characteristics such as associativity. Interoperability refers to the ability to share and communicate in the heterogeneous environment or heterogeneous soft | M W Fu W F Lu A Y C Nee S K Ong | 2002 | 厦门大学学报(自然科学版)2002，41，S1： | 2 |
| 5 | Moving element method for train-track dynamics显示文摘 | Koh C G Ong J S Y Chua D K H Feng J | 2003 | International Journal for Numerical Methods in Engineering2003，56，： | 1 |
| 6 | Above and below groundinteraction in agroforestry system s显示文摘 | Ong C K Corlett J E Singh R P | 1991 | Forest Ecology andManagement1991，45，1： | 1 |
| 7 | Magnetic properties of BaFe12-2xZnxZrxO19 particles显示文摘 | WEI F L FANG H C ONG C K | 2000 | J Appl Phys2000，87，： | 1 |
| 8 | Critical cooling rates for glass formation in Zr-Al-Cu-Ni alloys 显示文摘 | HNG H H LI Y NG S C ONG C K | 1996 | J Non-Cryst Solids1996，208，12： | 1 |
| 9 | Ferrocement laminates for strengthening RC T- beams 显示文摘 | Paramasivam P Ong K C G Lim C T E | 1994 | Cement and Concrete Composites1994，16，2： | 1 |
| 10 | Magnetic order in Co-doped and (Mn,Co) codoped ZnO thin films by pulsed laser eposition显示文摘 | YAN L ONG C K RAO X S | 2004 | Appl Phys2004，96，： | 1 |
| 11 | The development of microalbuminuria is associated with raised longitudinal adiponectin levels in female but not male adolescent patients with type 1 diabetes显示文摘 | Amin R Frystyk J Ong K | | 0，，： | 1 |
| 12 | Hybrid genetic algorithm and simulated annealing approach for the optimization of process plans for prismatic parts显示文摘 | LI W D ONG S K NEE A Y C | 2002 | International Journal of Product Research2002，40，8： | 1 |
| 13 | LXR, a nuclear receptor that defines a distinct retinoid response pathway显示文摘 | Willy PJ Umesono K Ong ES | 1995 | Genes Dev1995，9，9： | 1 |
| 14 | Nitrogen dy namics in cropping systems in southern Malawi contai ning Gliricidia sepium, pigeon peas and maize 显示文摘 | Chirwa P W Black C R Ong C K | 2006 | Agroforestry Systems2006，67，： | 1 |
| 15 | Glass forming ability of bulk glass forming alloys显示文摘 | Li Y Ng S N Ong C K | 1997 | Scripta Material1997，36，7： | 1 |
| 16 | Low temperature character/zation of nano-sized BaFe12-2xZnxSnxO19 particlesJournal of Magnetism and Magnetic Materials显示文摘 | Fang H C Ong C K Zhang X Y | 1999 | Journal of Magnetism and Magnetic Materials1999，191，： | 1 |
| 17 | Flexural Strengthening of Reinforced Concrete Beams Using Ferrocement Laminate 显示文摘 | Ong K C G Paramasivam Lim C T E | 1992 | Journal of Ferrocement1992，，： | 1 |
| 18 | Water-to-air mass transfer of VOCs: Lahoratory-scale air sparging system显示文摘 | Chao K P Ong S K Protopapas A | 1998 | Journal of Envi?ronmental Engineering1998，124，11： | 1 |
| 19 | Wastewater treatment with bacterial augmentation显示文摘 | Chin K K Ong S L Poh L H | 1998 | Water Sci Technol1998，33，8： | 1 |
| 20 | 显示文摘 | Ong H R Khan M R Chowdhury M N K | 2014 | Fuel2014，120，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员