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| 1 | Biomonitoring for traditional herbal medicinal products using DNA metabarcoding and single molecule, real-time sequencing显示文摘Global concerns have been paid to the potential hazard of traditional herbal medicinal products(THMPs). Substandard and counterfeit THMPs, including traditional Chinese patent medicine, health foods, dietary supplements, etc. are potential threats to public health. Recent marketplace studies using DNA barcoding have determined that the current quality control methods are not sufficient for ensuring the presence of authentic herbal ingredients and detection of contaminants/adulterants. An efficient biomonitoring method for THMPs is of great needed. Herein, metabarcoding and single-molecule, realtime(SMRT) sequencing were used to detect the multiple ingredients in Jiuwei Qianghuo Wan(JWQHW), a classical herbal prescription widely used in China for the last 800 years. Reference experimental mixtures and commercial JWQHW products from the marketplace were used to confirm the method. Successful SMRT sequencing results recovered 5416 and 4342 circular-consensus sequencing(CCS) reads belonging to the ITS2 and psb A-trn H regions. The results suggest that with the combination of metabarcoding and SMRT sequencing, it is repeatable, reliable, and sensitive enough to detect species in the THMPs, and the error in SMRT sequencing did not affect the ability to identify multiple prescribed species and several adulterants/contaminants. It has the potential for becoming a valuable tool for the biomonitoring of multi-ingredient THMPs. | Tianyi Xin Zhichao Xu Jing Jia Christine Leon Songnian Hu Yulin Lin Subramanyam Ragupathy Jingyuan Song Steven G.Newmaster | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 22 |
| 2 | In vitro antioxidant activities of ethanol extract from Enhalus acoroides (L.F.) Royle显示文摘Objective:To search for new sources of safe and inexpensive antioxidants,the leaf,root and rhizome of Enhalus acoroides(E.acoroides) were screened for the first time for their antioxidant activities.Methods:Samples of leaf,root and rhizome from E.acoroides were tested for total phenolic content,proanthocyanidins and in vitro antioxidant activity in terms of total antioxidant assay,DPPH assay and FRAP assay.Results:The leaf sample was found to have high levels of phenolic(0.323±0.028 mg TAE/g) and proanthocyanidins(0.570 0±0.000 3 mg TAE/g) when compared to root and rhizome.The leaf samples exhibited higher total antioxidant activity (11.770±0.026 mg Ascorbic acid equivalent/g),higher percentage of DPPH radical scavenging activity(25.76±0.04) and higher reducing power(18.060±0.073) in terms of mg GAE/g.In addition, there was a significant correlation between total phenolic content and total antioxidant activity (R^2=0.923),DPPH assay(R^2=0.509).FRAP assay(R^2=0.994).Similarly,significant correlation was found between the proanthocyanidin and total antioxidant activity(R^2=0.977),DPPH assay (R^2=0.464) and the FRAP assay(R^2=0.998).Conclusions:These results suggested that E.acoroides have strong antioxidant potential.Further study is necessary for isolation and characterization of the active antioxidant agents,which can be used to treat various oxidative stress-related diseases. | Rengasamy Ragupathi Raja Kannan Rajasekaran Arumugam Perumal Anantharaman | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，11： | 15 |
| 3 | Antibacterial potential of three seagrasses against human pathogens显示文摘Objective:To evaluate the antibacterial activity of Halophila stipulacea(H.stipulacea), Cymodocea serrulata(C.serrulata) and Halodule pinifolia(H.pinifolia) against seven human bacterial pathogens.Methods:The antibacterial activities of the extracts on the various test organisms using disc diffusion method and Minimum Inhibitory Concentraction(MIC).Results: Methanol and chloroform extracts of all the three seagrasses were active against all the tested pathogens,whereas the hexane extract of seagrasses was not active against Staphylococcus aureus (S.aureus).Antibacterial activity of three seagrass screened,was in the order of H.pinifolia > H.stipulacea.> C.serrulata.Conclusions:This antibacterial studies can further investigated on seagrasses for purification of bioactive substance and its possible utility in disease control. | Rengasamy Ragupathi Raja Kannan Rajasekaran Arumugam Perumal Anantharaman | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，11： | 7 |
| 4 | Stem cells:An eventual treatment option for heart diseases显示文摘Stem cells are of global excitement for various diseases including heart diseases. It is worth to understand the mechanism or role of stem cells in the treatment of heart failure. Bone marrow derived stem cells are commonly practiced with an aim to improve the function of the heart. The majority of studies have been conducted with acute myocardial infarction and a few has been investigated with the use of stem cells for treating chronic or dilated cardiomyopathy. Heterogeneity in the treated group using stem cells has greatly emerged. Ever increasing demand for any alternative made is of at most priority for cardiomyopathy. Stem cells are of top priority with the current impact that has generated among physicians. However,meticulous selection of proper source is required since redundancy is clearly evident with the present survey. This review focuses on the methods adopted using stem cells for heart diseases and outcomes that are generated so far with an idea to determine the best therapeutic possibility in order to fulfill the present demand. | Joseph C Bilgimol Subbareddy Ragupathi Lakshmanan Vengadassalapathy Nathan S Senthil Kali-muthu Selvakumar M Ganesan Sadananda Rao Manjunath | 2015 | World Journal of Stem Cells2015，7，8： | 6 |
| 5 | Induction of antibodies against GD3 ganglioside in melanoma patients by vaccination with GD3-1actone-KLH conjugate plus immunological adjuvant QS-21 显示文摘 | Ragupathi G Meyers M Adluri S Howard L Musselli C Livingston PO | 2000 | Int J Cancer2000，85，5： | 1 |
| 6 | Reevaluation of the cellular immune response in breast cancer patients vaccinated with MUC1显示文摘 | Musselli C Ragupathi G Gilewski T | 2002 | Int J Cancer2002，97，5： | 1 |
| 7 | Synthesis and characterization of nano-MnO2 for electrochemical supercapacitor studies显示文摘 | RAGUPATHY P VASAN H N | | 0，，1： | 1 |
| 8 | DNA barcoding in the land plants: evaluation of rbcL in a multigene tiered approach 显示文摘 | Newmaster S G Fazekas A J Ragupathy S | 2006 | Candian Joural of Botany2006，84，： | 1 |
| 9 | Consistent antibody response against ganglio-side GD2 induced in patients with melanoma by a GD2 lactonekeyhole limpet hemoeyanin conjugate vaccine plus immunological adjuvant QS-21 显示文摘 | Ragupathi G Livingston PO Hood C Gathuru J Krown SE Chapman PB | 2003 | Clin Cancer Res2003，9，14： | 1 |
| 10 | Vaccination of small cell lung cancer patients with polysialic acid or N-Propionylated polysialic acid conjugated to Keyhole Limpet Hemoeyanin显示文摘 | Krug LM Ragupathi G Ng KK | 2004 | Clin Cancer Res2004，10，： | 1 |
| 11 | Vaccination of patients with small-cell lung cancer with Synthetic Fucosy1 GM-1 conjugated to Keyhole Limpet Hemocyanin显示文摘 | Krug LM Ragupathi G Hood C | 2004 | Clinical Cancer Res2004，10，： | 1 |
| 12 | Vaccination of small cell lung cancer patients with polysialic acid or N-propionylated polysialic acid conjugated to keyhole limpet hemocyanin 显示文摘 | Krug LM Ragupathi G Ng KK | 2004 | Clin Cancer Res2004，10，3： | 1 |
| 13 | Immunization with N-propionyl polysialie acid-KLH conjugate in patients with small cell lung Cancer is safe and induces IgM antibodies reactive with SCLC cells amt bactericidal against group B meningocoeci 显示文摘 | Krug LM Ragupathi G Hood C | 2012 | Cancer hnmunol hnmunother2012，61，1： | 1 |
| 14 | Effective extraction of heavy metals from their effluents using some potential ionic liquids as green chemicals显示文摘 | Rajendran A Ragupathy D Priyadarshini M | 2011 | E-Journal of Chemistry2011，8，2： | 1 |
| 15 | The known immunologically active components of astragalus account for only a small proportion of the immunological adjuvant activity when combined with conjugate vaccines显示文摘 | Hong F Xiao W Ragupathi G | 2007 | Planta Med2007，，3： | 1 |
| 16 | Comparison of the effect of different immunological adjuvants on the antibody and T-cell response to immunization with MUC1-KLH and GD3-KLH conjugate cancer vaccines 显示文摘 | Kim SK Ragupathi G Musselli C | 1999 | Vaccine1999，18，78： | 1 |
| 17 | Effect of immunological adjuvant combinations on the antibody and T-cell response to vaccination with MUC1-KLH and GD3-KLH conjugates 显示文摘 | Kim SK Ragupathi G Cappello S | 2000 | Vaccine2000，19，45： | 1 |
| 18 | DNA barcoding in the land plants: evaluation of rbcL in a multigene tiered approach 显示文摘 | Newmaster S G Fazekas A J Ragupathy S | 2006 | Candian Joural of Botany2006，84，： | 1 |
| 19 | Identification of new,emerging HIV-1 unique recombinant forms and drug resistant viruses circulating in Cameroon显示文摘 | Ragupathy V Zhao J Wood O | | 0，，： | 1 |
| 20 | Vaccination of small cell lung cancer patients with polysialic acid or N-propionylated polysialic acid conjugated to keyhole limpet hemocyanin显示文摘 | Krug L M Ragupathi G Ng K K | 2004 | Clin Cancer Res2004，10，： | 1 |
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