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| 1 | Tumor suppressor ASXL1 is essential for the activation of INK4B expression in response to oncogene activity and anti-proliferative signals显示文摘ASXL1 变化经常在 hematological 肿瘤被发现,并且 Asxl1 的损失在老鼠支持 myeloid 转变。这里我们在 vivo 在在 Histone H2A (H2AK119ub1 ) 上的 mono-ubiquitylated 离氨酸 119 的 deubiquitylation 为 ASXL1-BAP1 建筑群支持一个角色的现在的数据。Polycomb 组蛋白质在正常开发期间控制 INK4B-ARF-INK4A 地点的表达式,部分地通过催化 H2AK119 的 mono-ubiquitylation。自从地点的激活, INK4B-ARF-INK4A 玩一设有自动保险装置机制免于 tumorigenesis,我们调查了 ASXL1 依赖的 H2A deubiquitylation 是否在它的激活起一个作用。有趣地,我们发现 ASXL1 明确地为 p15 响应 oncogenic 发信号和外来的 anti-proliferative 的 INK4B 发信号。因为我们发现 ASXL1 和 BAP1 两个都在 INK4B 地点被充实,我们的结果建议 INK4B 地点的激活要求 H2AK119ub1 的 ASXL1/BAP1-mediated deubiquitylation。一致地,我们 ASXL1 变化与被联系的结果表演降低 p15 INK4B 和在主要骨头髓房间的造血的祖先的一个 proliferative 优点,和在多重房间线的 ASXL1 的那弄空导致抵抗到生长禁止的信号。一起拿,这研究连接 INK4B 表示的调停 ASXL1 的 H2A deubiquitylation 和 transcriptional 激活到它的肿瘤 suppressor 函数。 | Xudong Wu Ida Holst Bekker-Jensen Jesper Christensen Kasper Dindler Rasmussen Simone Sidoli Yan Qi Yu Kong Xi Wang Yajuan Cui Zhijian Xiao Guogang Xu Kristine Williams Juri Rappsilber Casper Kaae Sonderby Ole Winther Ole N Jensens Kristian Helin | 2015 | Cell Research2015，25，11： | 3 |
| 2 | A generic strategy to analyze the spatial organization of multi-protein complexes by cross- linking and mass spectrometry显示文摘 | Rappsilber J Siniossoglou S Hurt E C | 2000 | Anal Chem2000，72，2： | 1 |
| 3 | Modular stop and go ex- traction tips with stacked disks for parallel and muhidimen- sional peptide fractionation in proteomics显示文摘 | Ishihama Y Rappsilber J Mann M | 2006 | J Proteome Res2006，5，4： | 1 |
| 4 | Experience and perspectives of MALDI MS and MS/MS in proteomic research显示文摘 | Rappsilber J Moniatte M Nielsen M L | 2003 | Int J Mass Spectrometry2003，226，1： | 1 |
| 5 | Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectro- spray, and LC/MS sample pretreatment in proteomics显示文摘 | Rappsilber J Ishihama Y Mann M | 2003 | Anal Chem2003，75，3： | 1 |
| 6 | Model for stathmin/Op18 binding to tubulin 显示文摘 | Wallon G Rappsilber J Mann M | 2000 | EMBO2000，19，2： | 1 |
| 7 | Protocol for micro-purifi- cation, enrichment, pre-ractionation and storage o peptides for proteomics using StageTips显示文摘 | Rappsilber J Mann M Ishihama Y | 2007 | Nat Protocols2007，2，8： | 1 |
| 8 | A programmed wave of uridylation-primed mRNA degradation is essential for meiotic progression and mammalian spermatogenesis显示文摘Several developmental stages of spermatogenesis are transcriptionally quiescent which presents major challenges associated with the regulation of gene expression. Here we identify that the zygotene to pachytene transit!on is not only associated with the resumption of transcription but also a wave of programmed mRNA degradation that is essential for meiotic progress!on. We explored whether terminal uridydyl transferase 4-(TUT4-) or TUT7-mediated 3' mRNA uridylation contributes to this wave of mRNA degradation during pachynema. Indeed, both TUT4 and TUT7 are expressed throughout most of spermatogenesis, however, loss of either TUT4 or TUT7 does not have any major impact upon spermatogenesis. Combined TUT4 and TUT7 (TUT4/7) deficiency results in embryonic growth defects, while conditional gene targeting revealed an essential role for TUT4/7 in pachytene progression. Loss of TUT4/7 results in the reduction of miRNA, piRNA and mRNA 31 uridylation. Although this reduction does not greatly alter miRNA or piRNA expression, TUT4/7-mediated uridylation is required for the clearanee of many zygotene-expressed transcripts in pachytene cells. We find that TUT4/7-regulated transcripts in pachytene spermatocytes are characterized by having long 3Z UTRs with length-adjusted enrichment for AU-rich elements. We also observed these features in TUT4/7-regulated maternal transcripts whose dosage was recently shown to be essential for sculpting a functional maternal transcriptome and meiosis. Therefore, mRNA 3Z uridylation is a critical determinant of both male and female germline transcriptomes. In conclusion, we have identified a novel requirement for 3' uridylation-programmed zygotene mRNA clearanee in pachytene spermatocytes that is essential for male meiotic progression. | Marcos Morgan Yuka Kabayama Christian Much Ivayla Ivanova Monica Di Giacomo Tatsiana Auchynnikava Jack Michael Monahan Dimitrios Michael Vitsios Lina Vasiliauskaite Stefano Comazzetto Juri Rappsilber Robin Campbell Allshire Bo Torben Porse Anton James Enright Donal O'Carroll | 2019 | Cell Research2019，29，3： | 1 |
| 9 | Protocol for micro- purification,enrichment,pre-fractionation and storage of peptides for proteomics using stage tips 显示文摘 | Rappsilber J Mann M Ishihama Y | 2007 | Nat Protoc2007，2，8： | 1 |
| 10 | What does it mean to identify a protein in proteomics? 显示文摘 | Rappsilber J Mann M | 2002 | Trends Biochem Sci2002，27，2： | 1 |
| 11 | What does it mean to identify a protein in proteomics?显示文摘 | Rappsilber J Mann M | 2002 | Trends Biochem Sci2002，27，2： | 1 |
| 12 | A generic strategy to analyze the spatial organization of multi-protein complexes by cross-linking and mass spectrometry 显示文摘 | Siniossogou S Hurt EC | 2000 | Anal Chem2000，72，： | 1 |
| 13 | Model for stathmin/Op18 binding to tubulin 显示文摘 | Rappsilber J Mann M | 2000 | EMBO2000，19，2： | 1 |
| 14 | Model for stathmin/Op18 binding to tubulin显示文摘 | Wallon G Rappsilber J Mann M | 2000 | EMBO2000，19，2： | 1 |
| 15 | Model for stathmin/OP18 binding to tubulin显示文摘 | RAPPSILBER J MANN M | 2000 | J EMRO2000，19，： | 1 |
| 16 | What does it mean to identify a protein in proteomics?显示文摘 | Rappsilber J Mann M | 2002 | Trends Biochem Sci2002，27，2： | 1 |
| 17 | What does it mean to identify a protein in proteomics显示文摘 | Rappsilber J Mann M | 2002 | Trends Biochemistry Science2002，7478，： | 1 |
| 18 | Experiences and perspectives of MALD1 MS and MS/MS in proteomic research 显示文摘 | Rappsilber J Moniatte M Nielsen ML | 2003 | International J Mass Spectrometry2003，226，1： | 1 |
| 19 | Model for stathmin/OP18 binding to tubulin显示文摘 | WALLON G RAPPSILBER J MANN M | 2000 | EMBO J2000，19，2： | 1 |
| 20 | BoxPlotR: a web tool for generation of box plots 显示文摘 | SPITZER M WILDENHAIN J RAPPSILBER J | 2014 | Nat Methods2014，11，2： | 1 |
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