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| 1 | Chronic exposure to ethanol causes steatosis and inflammation in zebrafish liver显示文摘AIM To evaluate the effects of chronic exposure to ethanol in the liver and the expression of inflammatory genesin zebrafish.METHODS Zebrafish(n = 104),wild type,adult,male and female,were divided into two groups:Control and ethanol(0.05 v/v).The ethanol was directly added into water;tanks water were changed every two days and the ethanol replaced.The animals were fed twice a day with fish food until satiety.After two and four weeks of trial,livers were dissected,histological analysis(hematoxilineosin and Oil Red staining) and gene expression assessment of adiponectin,adiponectin receptor 2(adipor2),sirtuin-1(sirt-1),tumor necrosis factor-alpha(tnf-a),interleukin-1b(il-1b) and interleukin-10(il-10) were performed.Ultrastructural evaluations were conducted at fourth week.RESULTS Exposing zebrafish to 0.5% ethanol developed intense liver steatosis after four weeks,as demonstrated by oil red staining.In ethanol-treated animals,the main ultrastructural changes were related to cytoplasmic lipid particles and droplets,increased number of rough endoplasmic reticulum cisterns and glycogen particles.Between two and four weeks,hepatic mR NA expression of il-1b,sirt-1 and adipor2 were upregulated,indicating that ethanol triggered signaling molecules which are key elements in both hepatic inflammatory and protective responses.Adiponectin was not detected in the liver of animals exposed and not exposed to ethanol,and il-10 did not show significant difference.CONCLUSION Data suggest that inflammatory signaling and ultrastructural alterations play a significant role during hepatic steatosis in zebrafish chronically exposed to ethanol. | Ana Claudia Reis Schneider Cleandra Gregório Carolina Uribe-Cruz Ranieli Guizzo Tais Malysz Maria Cristina Faccioni-Heuser Larisse Longo Themis Reverbel da Silveira | 2017 | World Journal of Hepatology2017，9，8： | 7 |
| 2 | Bone mineral density in cone beam computed tomography:Only a few shades of gray显示文摘Cone beam computed tomography(CBCT) has often been used to determine the quality of craniofacial bone structures through the determination of mineral density, which is based on gray scales of the images obtained. However, there is no consensus regarding the accuracy of the determination of the gray scales in these exams. This study aims to provide a literature review concerning the reliability of CBCT to determine bone mineral density. The gray values obtained with CBCT show a linear relationship with the attenuation coefficients of the materials, Hounsfield Units values obtained with medical computed tomography, and density values from dual energy X-ray absorciometry. However, errors are expected when CBCT images are used to define the quality of the scanned structures be-cause these images show inconsistencies and arbitrari-ness in the gray values, particularly when related to abrupt change in the density of the object, X-ray beam hardening effect, scattered radiation, projection data discontinuity-related effect, differences between CBCTdevices, changes in the volume of the field of view(FOV), and changes in the relationships of size and position between the FOV and the object evaluated. A few methods of mathematical correction of the gray scales in CBCT have been proposed; however, they do not generate consistent values that are independent of the devices and their configurations or of the scanned objects. Thus, CBCT should not be considered the ex-amination of choice for the determination of bone and soft tissue mineral density at the current stage, par-ticularly when values obtained are to be compared to predetermined standard values. Comparisons between symmetrically positioned structures inside the FOV and in relation to the exomass of the object, as it occurs with the right and left sides of the skull, seem to be viable because the effects on the gray scale in the re-gions of interest are the same. | Marcio José da Silva Campos Thainara Salgueiro de Souza Sergio Luiz Mota Júnior Marcelo Reis Fraga Robert Willer Farinazzo Vitral | 2014 | World Journal of Radiology2014，6，8： | 3 |
| 3 | Cirrhosis in children and adolescents: An overview显示文摘Several conditions, especially chronic liver diseases, can lead to cirrhosis in children and adolescents. Most cases in clinical practice are caused by similar etiologies. In infants, cirrhosis is most often caused by biliary atresia and genetic-metabolic diseases, while in older children, it tends to result from autoimmune hepatitis,Wilson's disease, alpha-1-antitrypsin deficiency and primary sclerosing cholangitis. The symptoms of cirrhosis in children and adolescents are similar to those of adults. However, in pediatric patients, the first sign of cirrhosis is often poor weight gain. The complications of pediatric cirrhosis are similar to those observed in adult patients, and include gastrointestinal bleeding caused by gastroesophageal varices, ascites and spontaneous bacterial peritonitis. In pediatric patients, special attention should be paid to the nutritional alterations caused by cirrhosis, since children and adolescents have higher nutritional requirements for growth and development. Children and adolescents with chronic cholestasis are at risk for several nutritional deficiencies. Malnutrition can have severe consequences for both pre- and post-liver transplant patients. The treatment of cirrhosis-induced portal hypertension in children and adolescents is mostly based on methods developed for adults. The present article will review the diagnostic and differential diagnostic aspects of end-stage liver disease in children, as well as the major treatment options for this condition. | Raquel Borges Pinto Ana Claudia Reis Schneider Themis Reverbel da Silveira | 2015 | World Journal of Hepatology2015，7，3： | 3 |
| 4 | N-acetylcysteine modulates angiogenesis and vasodilation in stomach such as DNA damage in blood of portal hypertensive rats显示文摘AIM: To evaluate the antioxidant effect of N-acetylcysteine(NAC) on the stomach of rats with portal hypertension.METHODS: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups(n =6 each): Sham-operated(SO),SO + NAC,partial portal vein ligation(PPVL),and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg(i.p.) diluted in 0.6 m L of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution(0.6 m L) for the same period of time as the PPVL + NAC and SO + NAC group. On the 15 th day the animals were anesthetized and we evaluated portal pressure by cannulating mesenteric artery. After,we removed the stomach for further analysis. We performed immunohistochemical analysis for endothelial nitric oxide synthase(e NOS),vascular endothelial growth factor(VEGF),and nitrotirosine(NTT) proteins in stomach. We also evaluated e NOS and VEGF by Western blot analysis and assessed DNA damage in blood samples by the comet assay.RESULTS: The portal hypertension group exhibited increases in portal pressure when compared to SO group(29.8 ± 1.8 vs 12.0 ± 0.3 mm Hg)(P < 0.001). The same was observed when we compared the e NOS(56.8 ± 3.7 vs 13.46 ± 2.8 pixels)(P < 0.001),VEGF(34.9 ± 4.7 vs 17.46 ± 2.6 pixels)(P < 0.05),and NTT(39.01 ± 4.0 vs 12.77 ± 2.3 pixels)(P < 0.05) expression by immunohistochemistry of the PPVL animals with the SO group. The expression of e NOS(0.39 ± 0.03 vs 0.25 ± 0.03 a.μ)(P < 0.01) and VEGF(0.38 ± 0.04 vs 0.26 ± 0.04 a.μ)(P < 0.01) were also evaluated by Western blot analysis,and we observed an increase of both proteins on PPVL animals. We also evaluated the DNA damage by comet assay,and observed an increase on damage index and damage frequency on those animals. NAC decreased portal pressure values in PPVL + NAC animals(16.46 ± 2 vs 29.8 ± 1.8 mm Hg)(P < 0.001) when compared to PPVL. The expression of e NOS(14.60 ± 4.1 vs 56.8 ± 3.7 pixels)(P < 0.001),VEGF(19.53 ± 3.2 vs 34.9 ± 4.7 pixels)(P < 0.05) and NTT(21.84 ± 0.7 vs 39.01 ± 4.0 pixels)(P < 0.05) evaluated by immunohistochemistry were also reduced in PPVL + NAC animals. Also,when evaluated by Western blot e NOS expression(0.32 ± 0.03 vs 0.39 ± 0.03 a.μ)(P < 0.05) and VEGF expression(0.31 ± 0.09 vs 0.38 ± 0.04 a.μ)(P < 0.01). Furthermore,NAC modulated DNA damage in PPVL + NAC animals.CONCLUSION: In view of these results,we believe NAC is able to protect the stomach from the alterations induced by the PPVL procedure. | Francielli Licks Renata Minuzzo Hartmann Camila Marques Elizangela Schemitt Josieli Raskopf Colares Mariana do Couto Soares Juliana Reys Camila Fisher Juliana da Silva Norma Possa Marroni | 2015 | World Journal of Gastroenterology2015，21，43： | 2 |
| 5 | Acaricidal activity of Derris floribunda essential oil and its main constituent显示文摘Objective: To evaluate the acaricidal activity of the essential oil obtained from roots of Derris floribunda(D. floribunda)(Miq.) Benth, and its main constituent nerolidol against the Mexican mite Tetranychus mexicanus(T. mexicanus)(Mc Gregor).Methods: The essential oil from the roots of D. floribunda collected in the Amazon region(Brazil) was obtained by hydrodistillation. Its chemical composition was determined by GC–MS analysis. The acaricidal activities of this essential oil and nerolidol,were evaluated by recording the number of dead females(mortality) and eggs(fertility).Results: The essential oil showed sesquiterpenes as major volatile components. Nerolidol, the main component, represented 68.5% of the total composition of the essential oil. D. floribunda essential oil and nerolidol showed acaricidal activity, with LC_(50) of9.61 mg/m L air and 9.2 mg/m L air, respectively, over a 72 h period. In addition, both the essential oil and nerolidol significantly reduced the fecundity of T. mexicanus.Conclusions: Due to the economic importance of T. mexicanus and the lack of new pesticides, our data are very promising in the search for efficient and safer acaricidal products. Furthermore, this is the first report about the chemical composition and bioactivity of the essential oil of the Amazon plant species D. floribunda. | Ana Claudia Fernandes Amaral Aline de SRamos Marcia Reis Pena Jose Luiz Pinto Ferreira Jean Michel SMenezes Geraldo JNVasconcelos Neliton Marques da Silva Jefferson Rocha de Andrade Silva | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，9： | 2 |
| 6 | A structured proteomic approach identifies 14-3-3Sigma as a novel and reliable protein biomarker in panel based differential diagnostics of liver tumors 显示文摘 | Reis H Putter C Megger DA | 2015 | Biochim Biophys Acta2015，1854，6： | 1 |
| 7 | Leptospirosis显示文摘 | McBride AJ Athanazio DA Reis MG | 2005 | Curr Opin Infect Dis2005，18，5： | 1 |
| 8 | Feature extraction via multi-resolution analysis for short-term load forecasting显示文摘 | Rocha Reis A J Alves da Silva AP | 2005 | IEEE Transactions on Power Systems2005，20，1： | 1 |
| 9 | 3D integrated circuit layout visualization using VRML 显示文摘 | Indrusiak LS da Luz Reis RA | 2001 | Future Generation Computer Systems2001，17，5： | 1 |
| 10 | Biomimetic approach to ex- tensive fracture of anterior teeth-a case report显示文摘 | da Fonseca JT Reis JA Ribeiro CM | 2012 | Dent Trauma- tol2012，28，3： | 1 |
| 11 | Therapeutic window for treatment of cortical isehemia with bone marrow-derived cells in rats显示文摘 | de Vasconcelos Dos Santos A da Costa Reis J Diaz Paredes B | 2010 | Brain Res2010，1306，： | 1 |
| 12 | Analysis of bursttests and long-term hydrostatic tests in produced water pipelines显示文摘 | da Costa Mattos H S Paim L M Reis J M L | 2012 | Engineering Failure Analysis2012，22，： | 1 |
| 13 | Health-related quality of life in patients with systemic lupus erythematosus in Midwest Brazil显示文摘 | dos Reis MG da Costa IP | | 0，，04： | 1 |
| 14 | Effect of the number of screws on the stability of loc king mandibular reconstruction plates显示文摘 | PEREIRA-FILHO V A DA SILVA B N NUNES REIS J M | 2013 | International Jour nal of Oral and Maxillofacial Surgery2013，42，6： | 1 |
| 15 | Smart thermoresponsive coatings and surfaces for tissue engineering: switching cell-material boundaries显示文摘 | da Silva R M P Mano J F Reis R L | 2007 | Trends Bioteehn2007，25，12： | 1 |
| 16 | Serological screening and toxoplasmosis exposure factors among pregnant women in the Democratic Republic of Sao Tome and Principe显示文摘 | Chien-Ching Hung Chia-Kwung Fan Kua-Eyre Su Fung-Chang Sung Hung-Yi Chiou Vilfrido Gil Maria da Conceicao dos Reis Ferreira Jose Manuel de Carvalho Claudina Cruz Yu-Kuan Lin Lian-Fen Tseng Ke-Yun Sao Wen-Cheun Chang Hung-Shue Lan Shing-Hsien Chou | 2006 | Transactions of the Royal Society of Tropical Medicine and Hygiene2006，，2： | 1 |
| 17 | Percutaneous transluminal angioplasty in the treatment of stenosis of arteriovenous fistulae for hemodialysis显示文摘 | Miquelin DG Reis LF Da Silva AAM | 2008 | Internat Arch Med2008，1，： | 1 |
| 18 | Prevalence, Treatment, and Outcomes of the Hepatic Artery Stenosis After Liver Transplantation显示文摘 | R.F. da Silva R. Raphe H.C. Felício M.F. Rocha W.J. Duca P.C.J. Arroyo G.L. Palini A.M. Vasquez D.G. Miquelin L.F. Reis A.A.M. Silva R.C.M.A. da Silva | 2008 | Transplantation Proceedings2008，，3： | 1 |
| 19 | Ceramic veneers with minimum preparation显示文摘 | da Cunha LF Reis R Santana L | 2013 | Euro J Dent2013，7，4： | 1 |
| 20 | Neoadjuvant Radiother- apy Improves Survival in Patients With T2b/T3 Bladder Cancer: A Population-Based Analysis显示文摘 | Diaz DA Pollack A Reis IM | 2015 | Clin Genitourin Cancer2015，13，4： | 1 |
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