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| 1 | Correlation between rpoB gene mutation in Mycobacterium avium subspecies paratuberculosis and clinical rifabutin and rifampicin resistance for treatment of Crohn’s disease显示文摘AIM: To investigate overlapping regions of the rpoB gene previously involved with rifamycin resistance in M. tuberculosis and seek correlation between rpoB mutations in clinical MAP strains with susceptibility to RIF and RFB. METHODS: We designed a molecular-based PCR method for the evaluation of rifabutin (RFB) and rifampicin (RIF) resistance based on probable determinant regions within the rpoB gene of MAP, including the 81 bp variable site located between nucleotides 1363 and 1443. The minimum inhibitory concentration (MIC) for RIF was also determined against 11 MAP isolates in attempt to seek correlation with rpoB sequences. RESULTS: We determined that MAP strain 18 had an MIC of > 30 mg/L and ≤ 5 mg/L for RIF and RFB respectively, and a significant and novel rpoB mutation C1367T, compared to an MIC of ≤ 1.0 mg/L for both drugs in the wild type MAP. The 30-fold increase in the MIC was a direct result of the rpoB mutation C1367T, which caused an amino acid change Thr456 to Ile456 in the drug’s binding site. In addition, MAP strain 185 contained five silent rpoB mutations and exhibited an MIC comparable to the wild-type. Moreover, our in vitroselected mutation in MAP strain UCF5 resulted in the generation of a new resistant strain (UCF5-RIF16r) that possessed T1442C rpoB mutation and an MIC > 30 mg/L and > 10 mg/L for RIF and RFB respectively. Sequencing of the entire rpoB gene in MAP strains UCF4, 18, and UCF5-RIF16r revealed an rpoB mutation A2284C further downstream of the 81 bp variable region in UCF4, accounting for observed slight increase in MIC. In addition, no other significant mutations were found in strains 18 and UCF-RIF16r. CONCLUSION: The data clearly illustrates that clinical and in vitro-selected MAP mutants with rpoB mutations result in resistance to RIF and RFB, and that a single amino acid change in the beta subunit may have a significant impact on RIF resistance. Unconventional drug susceptibility testing such as our molecular approach will be beneficial for evaluation of antibiotic effectiveness. This molecular approach may also serve as a model for other drugs used for treatment of MAP infections. | Daniel R Beckler Sammer Elwasila George Ghobrial John F Valentine Saleh A Naser | 2008 | World Journal of Gastroenterology2008，14，17： | 2 |
| 2 | Blood classical monocytes phenotype is not altered in primary non-small cell lung cancer显示文摘AIM: To evaluate the M1 and M2 monocyte phenotype in patients with non-small cell lung cancer(NSCLC) compared to controls. Also, to examine the expression of Th1 and Th2 cytokines in plasma of NSCLC vs controls. METHODS: Freshly prepared peripheral blood mononuclear cells samples were obtained from patients with NSCLC(lung adenocarcinoma and squamous cell lung carcinoma) and from non-cancer controls. Flow cytometry was performed to investigate M1 and M2 phenotypes in peripheral monocytes(classical monocytes CD14+, CD45+ and CD16-) using conventional surface markers. Th1 and Th2 cytokine production was alsoanalysed in the plasma using cytometric bead array technique. RESULTS: There were no significant difference in expression of M1(HLA-DR) and/or M2 markers(CD163 and CD36) markers on classical monocytes in patients with NSCLC compared to non-cancer controls. Expression of CD11 b, CD11 c, CD71 and CD44 was also shown to be similar in patients with NSCLC compared to noncancer controls. Th1 and Th2 cytokines [interleukin(IL)-1β, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12(p70), tumor necrosis factor(TNF)-α, TNF-β, and interferon-γ] analysis revealed no significant difference between patients with NSCLC and non-cancer controls. CONCLUSION: This study shows no alteration in peripheral monocyte phenotype in circulating classical monocytes in patients with NSCLC compared to noncancer controls. No difference in Th1 and Th2 cytokine levels were noted in the plasma of these patients. | Saleh A Almatroodi Christine F Mc Donald Allison L Collins I an A Darby Dodie S Pouniotis | 2014 | World Journal of Clinical Oncology2014，5，5： | 2 |
| 3 | Efficacy of Gold Nanoparticles against Nephrotoxicity Induced by Schistosoma mansoni Infection in Mice显示文摘Objective In this study, the ameliorative effects of gold nanoparticles(gold NP) on the renal tissue damage in Schistosoma mansoni(S. mansoni)-infected mice was investigated. Methods High-resolution transmission electron microscopy was used for the characterization of NP. The gold NP at concentrations of 250, 500, and 1000 μg/kg body weight were inoculated into S. mansoni-infected mice. Results The parasite caused alterations in the histological architecture. Furthermore, it induced a significant reduction in the renal glutathione levels; however, the levels of nitric oxide and malondialdehyde were significantly elevated. The parasite also managed to downregulate KIM-1, NGAL, MCP-1, and TGF-β mR NA expression in infected animals. Notably, gold NP treatment in mice reduced the extent of histological impairment and renal oxidative damage. Gold NP were able to regulate gene expression impaired by S. Mansoni infection. Conclusion The curative effect of gold NP against renal toxicity in S. mansoni-infected mice is associated with their role as free radical scavengers. | Mohamed A Dkhil Mona F Khalil2 Amira A Bauom Marwa SM Dia Saleh A1-Qura | 2016 | Biomedical and Environmental Sciences2016，29，11： | 2 |
| 4 | Phase Ⅱ study of arsenic trioxide in patients with relapsed or refractory multiple myeloma显示文摘 | Hussein M A Saleh M Ravandi F | 2004 | Br J Haematol2004，125，4： | 1 |
| 5 | Reliability and validityof non-invasive imaging of internal carotid artery pseudo-occlusion显示文摘 | Fiirst G Saleh A Wenserski F | 1999 | Stroke1999，30，7： | 1 |
| 6 | Flavone C-glycosides from seeds of fenugreek, Trigonellafoenum-graecum L显示文摘 | Saleh R Torgils F Oyvind M A | 2010 | Journal of Agricultural and Food Chemistry2010，58，： | 1 |
| 7 | Flavonoids of Phlomis aurea 显示文摘 | Ei-Negoumy S I Abdalla M F Saleh N A M | 1986 | Phytochemstry1986，25，3： | 1 |
| 8 | The distally based sural artery flap for ankle and foot coverage显示文摘 | Cheema T A Saleh E S De Carvalho A F | 2007 | J Foot Ankle Surg2007，46，1： | 1 |
| 9 | Phase 2 study of arsenic troxide in patients with relapsed or refractory multiple myeloma显示文摘 | HUSSEIN M A SALEH M RAVANDI F | 2004 | Haemata2004，125，： | 1 |
| 10 | Laparoscopic management of non-communicating rudimentary horn in a dysmenorrheic and infertile patient显示文摘 | Saleh A M Sultan S F Al-Jawad H M | 2003 | Saudi Med J2003，24，2： | 1 |
| 11 | Carbohydrase are digested by proteases present in enzyme preparation during in vitro digestion显示文摘 | SALEH F OHTSUKA A TANAKA T | 2004 | Journal of Poultry Science2004，41，： | 1 |
| 12 | Inhibition of C6 glioma cells in vivo by expressionof antesense vascular endothelial growth factor sequence显示文摘 | Saleh M Stacker S A Wilks A F | 1996 | Cancer Res1996，56，： | 1 |
| 13 | Use of chargetransfer complexation in the spectrophotometric analysis of certain cephalosporins显示文摘 | Gamal A Saleh Hassan F Askal Mohamed F Rad- wan | 2001 | Talanta2001，54，： | 1 |
| 14 | Reliability and validity of noninvasive imaging of internal carotid artery pseudo-occlusion显示文摘 | G Furst A Saleh F Wenserski | 1999 | Stroke1999，30，7： | 1 |
| 15 | Effects of endo- thelial cells on human mesenchymal stem cell activity in a three-dimensional in vitro model显示文摘 | SALEH F A WHYTE M GENEVER P G | 2011 | Eur Cell Mater2011，22，： | 1 |
| 16 | Abnormal blood levesl of trace el- ements and metals , DNA damage, and breast csancer in the state of Kuwait显示文摘 | Saleh F Behbehani A Asfar S | 2011 | Biological Trace Element Res2011，141，1： | 1 |
| 17 | Photonic Boson Sampling in a Tunable Circuit显示文摘 | Matthew A B Alessandro F Saleh R K | 2013 | Science2013，339，： | 1 |
| 18 | Pectinase plays an important role in stimulating digestibility of a corn-soybean meal diet in broilers显示文摘 | TAHIR M SALEH F OHTSUKA A | 2006 | The Journal of Poultry Science2006，43，4： | 1 |
| 19 | Abnormal bloodlevesl of trace elements and metals,DNA damage, and breastcsancer in the state of Kuwait显示文摘 | SALEH F BEHBEHANI A ASFAR S | 2011 | Biological Trace Element Res2011，141，1： | 1 |
| 20 | Inhibition of growth of C6 glioma cells in vivo by expression of antisense vaseular endothelial growth factor sequenee显示文摘 | Saleh M Stacker S A Wilks A F | 1996 | Cancer Res1996，56，2： | 1 |
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