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| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | Development of hepatorenal syndrome in bile duct ligated rats显示文摘AIM: To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS: Male Wistar rats were submitted to sham-surgery or bile duct ligation (BDL) and divided according to the post-procedure time (2, 4 and 6-wk). To determine renal function parameters, rats wereplaced in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS: Rats with 2 wk of BDL increased free water clearance (P = 0.02), reduced urinary osmolality (P = 0.03) and serum creatinine (P = 0.01) in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including signif icant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic f ibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION: Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS. | Regina M Pereira Robson AS dos Santos Eduardo A Oliveira Virgínia HR Leite Filipi LC Dias Alysson S Rezende Lincoln P Costa Lucíola S Barcelos Mauro M Teixeira Ana Cristina Sim■es e Silva | 2008 | World Journal of Gastroenterology2008，14，28： | 2 |
| 3 | Purification of metallurgical grade silicon by acid leaching显示文摘 | SANTOS I C GONCALVES A P SANTOS C S ALMEIDA M AFONSO M H CRUZ M J | 1990 | Hydrometallurgy1990，23，： | 1 |
| 4 | Retention of vitamin C in drying processes of fruits and vegetables-A review 显示文摘 | SANTOS P H S SILVA M A | 2008 | Drying Technology2008，26，： | 1 |
| 5 | Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnides complex)to inorginanic pollu- tants exposure显示文摘 | Lopes P A Pinheiro T Santos M C | 2001 | Sci Total Environ2001，280，13： | 1 |
| 6 | The evolution of robotics research 显示文摘 | Garcia E Jimenez M A Santos P G D | 2007 | IEEE Robotics and Automation Society2007，4，1： | 1 |
| 7 | Polymerization of ethylene: some aspects of metallocene cata- lyst stabilization under homogeneous and heterogeneous reac- tion conditions显示文摘 | de Freitas A J D dos Santos J H Z Meneghetti S M P | 2011 | J Appl Poiym Sci2011，119，5： | 1 |
| 8 | Polymeric precursor method to the synthesis of XWO4(X = Ca and Sr)thin films-structural,micro-structural and spectroscopic investigations显示文摘 | Pontes F M Galhiane M S Santos L S Petit L A Kataoka F P Mabuchi G H Longo E Zampieri M Pi-zani P S | 2009 | Journal of Alloys and Compounds2009，477，12： | 1 |
| 9 | Sequential determination of fat- and water-soluble vitamins in green leafy vegetables during storage显示文摘 | Santos J Mendiola J A Oliveira M B P P | 2012 | Chromatogr A2012，1261，： | 1 |
| 10 | Fisheries oceanography using satellite and airhome remote sensing methods: a review显示文摘 | Santos A M P | 2000 | Fisheries Research2000，49，： | 1 |
| 11 | Effect of cigarette smoking on serum alpha-tocopherol and the lipid profile in a Portuguese population显示文摘 | LOPES P A SANTOS M C VICENTE L VIEGAS-CRESPO A M | 2004 | Clin Chim Acta2004，348，12： | 1 |
| 12 | Involvement of 5-HT re- ceptors in the antidepressant-like effect of adenosine in the mouse forced swimming test显示文摘 | Kaster M P Santos A' R Rodrigues A L | 2005 | Brain Res Bu112005，67，12： | 1 |
| 13 | Retrogression and re-aging of 7075 aluminium alloy: Microstructural characterization显示文摘 | VIANA F PINTO A M P SANTO H M C LOPES A B | 1999 | Materials Processing Technology1999，9293，： | 1 |
| 14 | Oxidation of CO,ethanol and toluene over TiO2 supported noble metal catalysts显示文摘 | SANTOS V P CARABINEIRO S A C TAVARES P B PEREIRA M F R ORFAO J J M FIGUEIREDO J L | | 0，，： | 1 |
| 15 | Synthesis of aNiFe204 catalyst for the preferential oxidation of carbon monoxide (PROX)显示文摘 | SANTOS P T A COSTA A C F M KIMINAMI R H G A ANDRADE H M C LIRA H L GAMA L | 2009 | Journal of Alloy and Compounds2009，483，12： | 1 |
| 16 | Biomarkers of exposure and effect as indicators of the interference of selenomethionine on methylmercury toxicity 显示文摘 | DOS SANTOS A P MATEUS M L CARVALHO C M | 2007 | Toxicol Lett2007，169，2： | 1 |
| 17 | Thermally induced threefold upconversion emission enhancement in nonresonant excited Er^3+/Yb^3+-codoped chalcogenide glass显示文摘 | dos SANTOS P V GOUVEIA E A de ARAUJO M T | 1999 | Appl Phys Lett1999，74，24： | 1 |
| 18 | Response of antioxidant enzymes in freshwater fish populations(Leuciscus alburnoidescomplex)to inorganic pollutants exposure显示文摘 | Lopes P A Pinheiro T Santos M C | 2001 | Science of the Total Environment2001，281，1： | 1 |
| 19 | Compositions and fluxes of particulate organic material in the Amazon River 显示文摘 | Hedges J I Clark W A Quay P D Richey J E Devol A H Santos U M | 1986 | Limnol Oceanogr1986，31，4： | 1 |
| 20 | A real-time visualization tool for forest eccsystem management decision support显示文摘 | Falcao A O Santos M P D Borges J G | 2006 | Computers and Electronics in Agriculture2006，53，： | 1 |
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