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| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | Development of hepatorenal syndrome in bile duct ligated rats显示文摘AIM: To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS: Male Wistar rats were submitted to sham-surgery or bile duct ligation (BDL) and divided according to the post-procedure time (2, 4 and 6-wk). To determine renal function parameters, rats wereplaced in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS: Rats with 2 wk of BDL increased free water clearance (P = 0.02), reduced urinary osmolality (P = 0.03) and serum creatinine (P = 0.01) in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including signif icant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic f ibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION: Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS. | Regina M Pereira Robson AS dos Santos Eduardo A Oliveira Virgínia HR Leite Filipi LC Dias Alysson S Rezende Lincoln P Costa Lucíola S Barcelos Mauro M Teixeira Ana Cristina Sim■es e Silva | 2008 | World Journal of Gastroenterology2008，14，28： | 2 |
| 3 | Purification of metallurgical grade silicon by acid leaching显示文摘 | SANTOS I C GONCALVES A P SANTOS C S ALMEIDA M AFONSO M H CRUZ M J | 1990 | Hydrometallurgy1990，23，： | 1 |
| 4 | Pharmacoki-netics and bioavailability of doxycycline in turkeys显示文摘 | Santos M Vermeersch H Remon J P | 1996 | Chromatogr B1996，682，2： | 1 |
| 5 | Partial purification and characterization of a thermostable trypsin from pyloric caeca of Tambaoui (Colossoma macropomum)显示文摘 | Bezerra R S Santos J F Paiva P M G | 2001 | Journal of Food Biochemistry2001，25，： | 1 |
| 6 | Impacts of the European Emission Trading Scheme Directive and Permit Assignment Methods on the Spanish Electricity Sector显示文摘 | LINARES P SANTOS F J VENTOSA M LAPIEDRA L | 2006 | Energy Journal2006，27，1： | 1 |
| 7 | Mechanical properties of HSLA bainitic steel subjected to controlled rolling with accelerated cooling显示文摘 | RODRIGUES P C M PERELOMA E V SANTOS D B | 2000 | Materials Science and Engineering: A2000，283，： | 1 |
| 8 | Retention of vitamin C in drying processes of fruits and vegetables-A review 显示文摘 | SANTOS P H S SILVA M A | 2008 | Drying Technology2008，26，： | 1 |
| 9 | Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnides complex)to inorginanic pollu- tants exposure显示文摘 | Lopes P A Pinheiro T Santos M C | 2001 | Sci Total Environ2001，280，13： | 1 |
| 10 | Immunohisto-chemical evidence of PTEN in oral squamous cell carcinoma and its correlation with the histological malignancy grading system显示文摘 | Squarize C H Castilho R M Santos P D Jr | 2002 | J Oral Pathol Med2002，31，： | 1 |
| 11 | The evolution of robotics research 显示文摘 | Garcia E Jimenez M A Santos P G D | 2007 | IEEE Robotics and Automation Society2007，4，1： | 1 |
| 12 | Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratom- ileusis 显示文摘 | Serrao S Buratto L Lombardo G De Santo MP Ducoli P Lom- bardo M | 2012 | J Cataract Surg2012，38，8： | 1 |
| 13 | Structure Effect on Electrical Properties of ITO Films Prepared by RF Reactive Magnetron Sputtering显示文摘 | Meng L J Dos Santos M P | 1996 | Thin Solid Films1996，289，： | 1 |
| 14 | Polymerization of ethylene: some aspects of metallocene cata- lyst stabilization under homogeneous and heterogeneous reac- tion conditions显示文摘 | de Freitas A J D dos Santos J H Z Meneghetti S M P | 2011 | J Appl Poiym Sci2011，119，5： | 1 |
| 15 | Polymeric precursor method to the synthesis of XWO4(X = Ca and Sr)thin films-structural,micro-structural and spectroscopic investigations显示文摘 | Pontes F M Galhiane M S Santos L S Petit L A Kataoka F P Mabuchi G H Longo E Zampieri M Pi-zani P S | 2009 | Journal of Alloys and Compounds2009，477，12： | 1 |
| 16 | Sequential determination of fat- and water-soluble vitamins in green leafy vegetables during storage显示文摘 | Santos J Mendiola J A Oliveira M B P P | 2012 | Chromatogr A2012，1261，： | 1 |
| 17 | Formation of ultra- fine ferrite mierostructure in warm rolled and annealed C-Mn steel显示文摘 | Santos D B Bruzszek R K Rodrigues P C M | 2003 | Materials Science and Engineering: A2003，346，12： | 1 |
| 18 | Non- Frankla actinomycetes isolated from surface-sterilized roots of Casuarina equisetifolia fix nitrogen显示文摘 | Valdes M Perez N O Estrada-de Los Santos P | 2005 | App! Environ Microbiol2005，71，1： | 1 |
| 19 | Fisheries oceanography using satellite and airhome remote sensing methods: a review显示文摘 | Santos A M P | 2000 | Fisheries Research2000，49，： | 1 |
| 20 | Comparison of three single-phase PLL algorithms for UPS applications显示文摘 | Santos Filho R M Seixas P F Cortizo P C | 2008 | IEEE Transactions on Industrial Electronics2008，55，8： | 1 |
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