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| 1 | ISSR markers based on GA and AG repeats reveal genetic relationship among rice varieties tolerant to drought,flood,or salinity显示文摘Drought, flood, salinity, or a combination of these limits rice production. Several rice varieties are well known for their tolerance to specific abiotic stresses. We determined genetic relationship among 12 rice varieties including 9 tolerant to drought, flood, or salinity using inter-simple sequence repeat (ISSR) markers. Based on all markers, the nine tolerant varieties formed one cluster distinct from the cluster of three control varieties. The salt-tolerant varieties were closest to two flood-tolerant varieties, and together they were distinct from the drought-tolerant varieties. (GA)8YG was the most informative primer, showing the highest polymorphic information content (PIC) and resolving power (Rp). The drought-, flood-, and salt-tolerant varieties grouped in three distinct clusters within the group of tolerant varieties, when (GA)8YG was used. Sabita was the only exception. The two aus varieties, Nagina22 and FR13A, were separated and grouped with the drought- and flood-tolerant varieties, respec- tively, but they were together in dendrograms based on other primers. The results show that ISSR markers associated with (GA)8YG delineated the three groups of stress-tolerant varieties from each other and can be used to identify genes/new alleles associated with the three abiotic stresses in rice germplasm. | Ch Surendhar REDDY A. Prasad BABU B.P. Mallikarjuna SWAMY K. KALADHAR N. SARLA | 2009 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2009，10，2： | 6 |
| 2 | QTL Analysis for Grain Iron and Zinc Concentrations in Two O. nivara Derived Backcross Populations显示文摘Identification of quantitative trait loci(QTLs) for grain mineral elements can assist in faster and more precise development of micronutrient dense rice varieties through marker-assisted breeding. In the present study, QTLs were mapped for Fe and Zn concentrations in two BC_2F_3 mapping populations derived from the crosses of O. sativa cv Swarna with two different accessions of O. nivara. In all, 10 and 8 QTLs were identified for grain Fe and Zn concentrations in population 1, and 7 and 5 QTLs were identified in population 2, respectively. Eighty percent of the QTLs detected in both populations were derived from O. nivara. Five QTLs for Fe and three QTLs for Zn explained more than 15% phenotypic variance either in interval or composite interval mapping. The locations of O. nivara derived QTLs such as qFe2.1,qFe3.1, qFe8.2 and qZn12.1 were consistently identified in both the populations. Epistatic interaction was observed only between RM106 and RM6 on chromosome 2 and between RM22 and RM7 on chromosome 3 for Fe concentration in population 1. Sixteen candidate genes for metal homeostasis were found to co-locate with 10 QTLs for Fe and Zn concentrations in both the populations. Most of the Fe and Zn QTLs were found to co-locate with QTLs for grain yield and grain quality traits. Some of the major effect QTLs identified can be used to improve rice grain Fe and Zn concentrations. | B. P. M. SWAMY K. KALADHAR1, K. ANURADHA Anil K. BATCHU T. LONGVAH N. SARLA | 2018 | Rice science2018，25，4： | 4 |
| 3 | Molecular Mapping of QTLs for Yield and Yield-Related Traits in Oryza sativa cv Swarna × O. nivara (IRGC81848) Backcross Population显示文摘Advanced backcross QTL analysis was used to identify QTLs for seven yield and yield-related traits in a BC2F2 population from the cross between a popular Oryza sativa cv Swarna and O. nivara IRGC81848. Transgressive segregants with more than 15% increased effect over Swarna were observed for all the traits except days to heading and days to 50% flowering. Thirty QTLs were detected for seven yield and yield-related traits using interval and composite interval mapping. Enhancing alleles at 13 (45%) of these QTLs were derived from O. nivara, and enhancing alleles at all the QTLs for stem diameter and rachis diameter were derived from O. nivara. Three stem diameter QTLs, two rachis diameter QTLs and one number of secondary branches QTL identified by both Interval and composite interval mapping contributed more than 15% of the total phenotypic variance. The QTL epistasis was significant for stem diameter and plot yield. The most significant QTLs qSD7.2, qSD8.1 and qSD9.1 for stem diameter, qRD9.1 for rachis diameter and qNSB1.1 for number of secondary branches are good targets to evaluate their use in marker-assisted selection. O. nivara is a good source of novel alleles for yield related traits and reveals major effect QTLs suitable for marker-assisted selection. | B. P. MALLIKARJUNA SWAMY K. KALADHAR M. S. RAMESHA B. C. VIRAKTAMATH N. SARLA | 2011 | Rice science2011，18，3： | 3 |
| 4 | Hirschsprung's disease:Is there a relationship between mast cells and nerve fibers?显示文摘AIM:To define the topography of mast cells and their numbers in cases of Hirschsprung's disease(HD)and non-HD,assess neural hypertrophy using imaging software and to study the relationship between mast cells and nerve fibers.METHODS:HE stained sections of 32 cases of chronic constipation in the age group of 0-14 years were reviewed for ganglion cells.AChE staining was performed on frozen sections of colonic and rectal biopsies.Based on their findings cases were divided into HD and non-HD and mast cells stained by toluidine blue were evaluated.Image analysis by computerized software was applied to S-100 stained sections for assessment of neural hypertrophy.RESULTS:Difference between number of mast cells in HD group(mean=36.44)and in non-HD group(mean =14.79)was statistically significant.Image analysis morphometry on S-100 stained sections served as a useful adjunct.The difference between number,size,and perimeter of the nerve fibers between HD and non-HD group was statistically significant.CONCLUSION:Mast cells are significantly increased in HD and their base line values are much higher in Indian children than that reported in Western literature.Their role in HD needs further research.Morphometry of S-100 stained nerve fibers is a useful adjunct to conventional methods for diagnosis of HD. | Amit Kumar Yadav Kiran Mishra Anup Mohta Sarla Agarwal | 2009 | World Journal of Gastroenterology2009，15，12： | 2 |
| 5 | Inter simple se- quence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 6 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | Reddy M P Sarla N Siddiq E A | 2002 | Euphytica2002，128，1： | 1 |
| 7 | Inter simple sequence repeat(ISSR) polymorphism and its application in plant breeding 显示文摘 | Reddy M P Sarla N Siddiq E A | 2002 | Euphytica2002，128，1： | 1 |
| 8 | Inter simple sequence repeat( IS- SR) polymorphism and its application in plant breeding显示文摘 | Reddy M P Sarla N Siddiq E A | 2002 | Eu- phytica2002，128，1： | 1 |
| 9 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding 显示文摘 | Reddy M P Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 10 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding 显示文摘 | Pradeep R M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 11 | Production of intergeneric hybrids between Sinapis alba and Brassica carinata显示文摘 | Sridevi1 O Sarla N | 2005 | Genetic Resources and Crop Evolution2005，52，： | 1 |
| 12 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | M. Pradeep Reddy N. Sarla E.A. Siddiq | 2002 | Euphytica2002，，1： | 1 |
| 13 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | Reddy M P Sarla N Siddiq E A | 2002 | Euphytica2002，128，1： | 1 |
| 14 | Inter simple sequence repeat(ISSR) polymorphism and its application in plant breeding显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 15 | Inter simple sequence repeat(ISSR) polymorphism and its application in plant breeding 显示文摘 | Pradeep Reddy M Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 16 | Inter simple sequence repeat (ISSR) polymorphixm and its application in plant breeding显示文摘 | Reddy M P Sarla N Siddiq E A | 2002 | Euphytica2002，128，： | 1 |
| 17 | Overcoming prefertilization barriers in the cross Diplotaxis siettiana & Brassica juncea Using iradiated mentor pollen 显示文摘 | SARMAH B K SARLA N | 1995 | Biologia Plantamm1995，37，3： | 1 |
| 18 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | M. Pradeep Reddy N. Sarla E.A. Siddiq | 2002 | Euphytica2002，，1： | 1 |
| 19 | Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding显示文摘 | Reddy MP Sarla N Siddiq EA | 2002 | Euphytica2002，128，1： | 1 |
| 20 | Inter simple sequence repeat (ISSR) polymorphism and its application in plantbreeding显示文摘 | Pradeep RM Sarla N Siddiq E A | 2002 | Eurphytica2002，128，： | 1 |
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