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| 1 | role of the normal gut microbiota显示文摘Relation between the gut microbiota and human health is being increasingly recognised. It is now well established that a healthy gut flora is largely responsible for overall health of the host. The normal human gut microbiota comprises of two major phyla, namely Bacteroidetes and Firmicutes. Though the gut microbiota in an infant appears haphazard, it starts resembling the adult flora by the age of 3 years. Nevertheless, there exist temporal and spatial variations in the microbial distribution from esophagus to the rectum all along the individual's life span. Developments in genome sequencing technologies and bioinformatics have now enabled scientists to study these microorganisms and their function and microbehost interactions in an elaborate manner both in health and disease. The normal gut microbiota imparts specific function in host nutrient metabolism, xenobiotic and drug metabolism, maintenance of structural integrity of the gut mucosal barrier, immunomodulation, and protection against pathogens. Several factors play a role in shaping the normal gut microbiota. They include(1) the mode of delivery(vaginal or caesarean);(2) diet during infancy(breast milk or formula feeds) and adulthood(vegan based or meat based); and(3) use of antibiotics or antibiotic like molecules that are derived from the environment or the gut commensal community. A major concern of antibiotic use is the long-term alteration of the normal healthy gut microbiota and horizontal transfer of resistance genes that could result in reservoir of organisms with a multidrug resistant gene pool. | Sai Manasa Jandhyala Rupjyoti Talukdar Chivkula Subramanyam Harish Vuyyuru Mitnala Sasikala D Nageshwar Reddy | 2015 | World Journal of Gastroenterology2015，21，29： | 187 |
| 2 | Pancreatic stellate cell: Pandora's box for pancreatic disease biology显示文摘Pancreatic stellate cells(PSCs) were identified in the early 1980 s, but received much attention after 1998 when the methods to isolate and culture them from murine and human sources were developed. PSCs contribute to a small proportion of all pancreatic cells under physiological condition, but are essential for maintaining the normal pancreatic architecture. Quiescent PSCs are characterized by the presence of vitamin A laden lipid droplets. Upon PSC activation, these perinuclear lipid droplets disappear from the cytosol, attain a myofibroblast like phenotype and expresses the activation marker, alpha smooth muscle actin. PSCs maintain their activated phenotype via an autocrine loop involving different cytokines and contribute to progressive fibrosis in chronic pancreatitis(CP) and pancreatic ductal adenocarcinoma(PDAC). Several pathways(e.g., JAK-STAT, Smad, Wnt signaling, Hedgehog etc.), transcription factors and mi RNAs have been implicated in the inflammatory and profibrogenic function of PSCs. The role of PSCs goes much beyond fibrosis/desmoplasia in PDAC. It is now shown that PSCs are involved in significant crosstalk between the pancreatic cancer cells and the cancer stroma. These interactions result in tumour progression, metastasis, tumour hypoxia, immune evasion and drug resistance. This is the rationale for therapeutic preclinical and clinical trials that have targeted PSCs and the cancer stroma. | Ratnakar R Bynigeri Aparna Jakkampudi Ramaiah Jangala Chivukula Subramanyam Mitnala Sasikala G Venkat Rao D Nageshwar Reddy Rupjyoti Talukdar | 2017 | World Journal of Gastroenterology2017，23，3： | 25 |
| 3 | Autologous mobilized peripheral blood CD34^+ cell infusion in non-viral decompensated liver cirrhosis显示文摘AIM: To study the effect of mobilized peripheral blood autologous CD34 positive(CD34+) cell infusion in patients with non-viral decompensated cirrhosis.METHODS: Cirrhotic patients of non-viral etiology were divided into 2 groups based on their willingness to be listed for deceased donor liver transplant(DDLT)(control, n = 23) or to receive autologous CD34+ cell infusion through the hepatic artery(study group, n= 22). Patients in the study group were admitted to hospital and received granulocyte colony stimulating factor injections 520 μg/d for 3 consecutive days to mobilize CD34+ cells from the bone marrow. On day 4,leukapheresis was done and CD34+ cells were isolated using CliniMAC magnetic cell sorter. The isolated CD34+ cells were infused into the hepatic artery under radiological guidance. The patients were discharged within 48 h. The control group received standard of care treatment for liver cirrhosis and were worked up for DDLT as per protocol of the institute. Both groups were followed up every week for 4 wk and then every month for 3 mo.RESULTS: In the control and the study group, the cause of cirrhosis was cryptogenic in 18(78.2%) and16(72.72%) and alcohol related in 5(21.7%) and6(27.27%), respectively. The mean day 3 cell count(cells/μL) was 27.00 ± 20.43 with a viability of 81.84± 11.99%. and purity of 80%-90%. Primary end point analysis revealed that at 4 wk, the mean serum albumin in the study group increased significantly(2.83± 0.36 vs 2.43 ± 0.42, P = 0.001) when compared with controls. This improvement in albumin was,however, not sustained at 3 mo. However, at the end of3 mo there was a statistically significant improvement in serum creatinine in the study group(0.96 ± 0.33 vs 1.42 ± 0.70, P = 0.01) which translated into a significant improvement in the Model for End-Stage Liver Disease score(15.75 ± 5.13 vs 19.94 ± 6.68,P = 0.04). On statistical analysis of secondary end points, the transplant free survival at the end of 1 mo and 3 mo did not show any significant difference(P =0.60) when compared to the control group. There was no improvement in aspartate transaminase, alanine transaminase, and bilirubin at any point in the study population. There was no mortality benefit in the study group. The procedure was safe with no procedural or treatment related complications.CONCLUSION: Autologous CD 34+ cell infusion is safe and effectively improves liver function in the short term and may serve as a bridge to liver transplantation. | Mithun Sharma Padaki Nagaraja Rao Mitnala Sasikala Mamata Reddy Kuncharam Chimpa Reddy Vardaraj Gokak BPSS Raju Jagdeesh R Singh Piyal Nag D Nageshwar Reddy | 2015 | World Journal of Gastroenterology2015，21，23： | 9 |
| 4 | Analgesic and anti-inflammatory activities of Passiflora foetida L.显示文摘Objective:To investigate the analgesic and anti-inflammatory activities of ethanol extract of Passiflora foetida(P.foetida) leaves.Methods:Ethanol extract of P.foetida leaf was evaluated for analgesic action by acetic acid-induced writhing and hot plate method in albino mice.The anti-inflammatory property of ethanolic leaf extract was tested by carrageenan induced acute paw edema and histamine induced acute paw edema in rats.Results:The dose 200 mg/kg of P. foetida leaf extract exhibited highest significant analgesic activity[(13.50±0.43) min]at a reaction time of 20 min in hot plate method in mice.The ethanol extract of leaf dose 100 mg/kg produced a highly significant anti inflammatory effect[(1.302±0.079) mL]in rats.Conclusions:It is very clear that P.foetida also has analgesic and anti-inflammatory activities for the pharmaceuticals. | Sasikala V Saravanan S Parimelazhagan T | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，8： | 7 |
| 5 | Pooled genetic analysis in ultrasound measured non-alcoholic fatty liver disease in Indian subjects:A pilot study显示文摘AIM: To investigate genetic susceptibility in Indian subjects with non-alcoholic fatty liver disease(NAFLD) by performing a pooled genetic study.METHODS: Study subjects(n = 306) were recruited and categorized into NAFLD and control groups based on ultrasound findings of fatty infiltration. Of the 306 individuals, 156 individuals had fatty infiltration and thus comprised the NAFLD group. One hundred and fifty(n = 150) individuals were normal, without fatty infiltration of the liver, comprising the control group. Blood samples, demographic and anthropometric data from the individuals were collected after obtaining informed consent. Anthropometric data, blood glucose, lipids and liver function tests were estimated using standard methods. Genome wide association stud-ies done to date on NAFLD were identified, 19 single nucleotide polymorphisms(SNPs) were selected from these studies that were reported to be significantly associated with NAFLD and genotyping was performed on the Sequenom platform. Student's t test for continuous variables and χ2 test was applied to variant carriers from both groups. Required corrections were applied as multiple testing was done.RESULTS The mean age of the control group was 39.78 ± 10.83 and the NAFLD group was 36.63 ± 8.20 years. The waist circumference of males and females in the control and NAFLD groups were 80.13 ± 10.35; 81.77 ± 13.65 and 94.09 ± 10.53; 92.53 ± 8.27 respectively. The mean triglyceride and alanine transaminase(ALT) levels in the control and NAFLD groups were 135.18 ± 7.77; 25.39 ± 14.73 and 184.40 ± 84.31; 110.20 ± 67.05 respectively. When χ2 test was applied to the number of individuals carrying the variant risk alleles between the control and NAFLD group, a significant association was seen between rs738409 of the patatin-like phospholipase domain containing 3(PNPLA3) gene(P = 0.001), rs2073080 of the PARVB gene(P = 0.02), rs2143571 of SAMM50 gene(P = 0.05) and rs6487679 of the pregnancy zone protein(PZP) gene(P = 0.01) with the disease. Variant single nucleotide polymorphisms(SNPs) in NCAN and PNPLA3 gene were associated with higher levels of ALT, whereas variant SNPs in APOC3, PNPLA3, EFCAB4 B and COL13A1 were associated with high triglyceride levels. Apart from the above associations, rs2073080, rs343062 and rs6591182 were significantly associated with high BMI; rs2854117 and rs738409 with high triglyceride levels; and rs2073080, rs2143571, rs2228603, rs6487679 and rs738409 with high ALT levels.CONCLUSION: Pooled genetic analysis revealed an association of SNPs in PNPLA3, PARVB, SAMM50 and PZP genes with NAFLD. SNPs in NCAN and PNPLA3gene were associated with higher levels of ALT,whereas variant SNPs in APOC3, PNPLA3, EFCAB4 B and COL13A1 were associated with high triglyceride levels. | Vishnubhotla Venkata Ravi Kanth Mitnala Sasikala Padaki Nagaraja Rao Urmila Steffie Avanthi Kalashikam Rajender Rao Duvvuru Nageshwar Reddy | 2014 | World Journal of Hepatology2014，6，6： | 6 |
| 6 | Thinking outside the liver: Induced pluripotent stem cells for hepatic applications显示文摘The discovery of induced pluripotent stem cells (iPSCs) unraveled a mystery in stem cell research, after identification of four re-programming factors for generating pluripotent stem cells without the need of embryos. This breakthrough in generating iPSCs from somatic cells has overcome the ethical issues and immune rejection involved in the use of human embryonic stem cells. Hence, iPSCs form a great potential source for developing disease models, drug toxicity screening and cell-based therapies. These cells have the potential to differentiate into desired cell types, including hepatocytes, under in vitro as well as under in vivo conditions given the proper microenvironment. iPSC-derived hepatocytes could be useful as an unlimited source, which can be utilized in disease modeling, drug toxicity testing and producing autologous cell therapies that would avoid immune rejection and enable correction of gene defects prior to cell transplantation. In this review, we discuss the induction methods, role of reprogramming factors, and characterization of iPSCs, along with hepatocyte differentiation from iPSCs and potential applications. Further, we discuss the location and detection of liver stem cells and their role in liver regeneration. Although tumor formation and genetic mutations are a cause of concern, iPSCs still form a promising source for clinical applications. | Mekala Subba Rao Mitnala Sasikala D Nageshwar Reddy | 2013 | World Journal of Gastroenterology2013，19，22： | 4 |
| 7 | B样条曲面拼接算法的设计与实现显示文摘B样条曲面拼接技术是计算机图形学研究的重要内容之一,曲面拼接技术也是国内外研究的热点之一。本文首先阐述B样条曲线、曲面的构造原理,设计实现了3次均匀B样条曲面和3次准均匀B样条曲面,构造了3片待拼接B样条曲面;其次通过待拼接曲面上边界曲线的型值点反算求出跨界曲线的控制点,生成跨界曲线,求出跨界曲线的跨界导矢;然后根据已知的映射和约束条件设计实现了基于N-1条边的拼接曲面,构建的拼接曲面插值于N-1条给定跨界曲线及其跨界导矢,曲面整体连续。运用此方法可以精确的表示规则曲面和自由曲面,实现了拼接曲面处处c 1连续;曲面形状便于修改和控制;算法运用C++语言和OpenGL函数,在Visual Studio 2010平台上进行调试、改进,验证了上述算法的可行性、有效性;给出了算法的运行结果。最后全文的工作、理论和实践意义进行了总结[1-5]。 | 吴丽娟 李博 ABEYSINGHE ARACHCHIGE Sasikala Sewwandi 张心慈 | 2019 | 沈阳师范大学学报(自然科学版)2019，37，6： | 4 |
| 8 | Genetics of non-alcoholic fatty liver disease: From susceptibility and nutrient interactions to management显示文摘Genetics plays an important role in determining the susceptibility of an individual to develop a disease. Complex, multi factorial diseases of modern day(diabetes, cardiovascular disease, hypertension and obesity) are a result of disparity between the type of food consumed and genes, suggesting that food which does not match the host genes is probably one of the major reasons for developing life style diseases. Non-alcoholic fatty liver is becoming a global epidemic leading to substantial morbidity. While various genotyping approaches such as whole exome sequencing using next generation sequencers and genome wide association studies have identified susceptibility loci for non-alcoholic fatty liver disease(NAFLD) including variants in patatin-like phospholipase domain containing 3 and transmembrane 6 superfamily member 2 genes apart from others; nutrient based studies emphasized on a combination of vitamin D, E and omega-3 fatty acids to manage fatty liver disease. However majority of the studies were conducted independent of each other and very few studies explored the interactions between the genetic susceptibility and nutrient interactions. Identifying such interactions will aid in optimizing the nutrition tailor made to an individual's genetic makeup, thereby aiding in delaying the onset of the disease and its progression. The present topic focuses on studies that identified the genetic susceptibility for NAFLD, nutritional recommendations, and their interactions for better management of NAFLD. | Vishnubhotla Venkata Ravi Kanth Mitnala Sasikala Mithun Sharma Padaki Nagaraja Rao Duvvuru Nageshwar Reddy | 2016 | World Journal of Hepatology2016，8，20： | 3 |
| 9 | Dynamic Fracture Toughness of Armour Grade Quenched and Tempered Steel Joints Fabricated Using Low Hydrogen Ferritic Fillers显示文摘The armour grade quenched and tempered steel joints fabricated using low hydrogen ferritic steel (LHF) filler exhibited superior joint efficiency owing to preferential ferrite microstructure in the welds and also they offered required resistance to HIC. However, the combat vehicles used in military operations will be required to operate under a wide range of road conditions ranging from first class to cross country. Structural components in combat vehicles are subjected to dynamic loading with high strain rates during operation. Stress loadings within the vehicle hull of these vehicles are expected to fluctuate considerably and structural cracking especially in welds during the service life of these vehicles can lead to catastrophic failures. Under these conditions, fracture behaviour of high strain rate sensitive structural steels can be better understood by dynamic fracture toughness (K1d). Hence, an attempt was made to study dynamic fracture toughness of the armour grade quenched and tempered steel and their welds fabricated using LHF consumables. The experimental results indicate that the K1d values of the joints fabricated by shielded metal arc welding (SMAW) are higher than those of the joints fabricated using flux cored arc welding (FCAW) process. | G Magudeeswaran V Balasubramanian S Sathyanarayanan G Madhusudhan Reddy A Moitra S Venugopal G Sasikala | 2010 | Journal of Iron and Steel Research(International)2010，17，5： | 2 |
| 10 | A cholesterol biosensor based on gold nanoparticles decorated functionalized graphene nanoplatelets显示文摘 | Aravind Sasidharan Sasikala Jyothirmayee Baby Aravind Tessy Theres Arockiadoss Thevasahayam | 2011 | Thin Solid Films2011，519，： | 1 |
| 11 | Electrodeposition and optical characterization of CdS thin films on ITO-coated glass显示文摘 | Dhnasekaran R Subramanian C | 1997 | Thin Solid Films1997，302，： | 1 |
| 12 | 5-Aminolevulinic acid:a potential herbicide/insecticide from microorganisms显示文摘 | SASIKALA C RAMANAV RAGHUVEER RAO P | 1994 | Biotechnol Prog1994，10，： | 1 |
| 13 | Modification in the chemical bath deposition apparatus,growth and characterization of CdS semiconducting thin films for Photovoltaic applications显示文摘 | SASIKALA G THILAKAN P SUBRAMANNIAN C | 2000 | Solar Energy Materials & Solar Cells2000，62，3： | 1 |
| 14 | 5-aminolevulinic Acid: A Potential Herbicide Insecticide from Microorganisms显示文摘 | Sasikala C Ramana C V Rao P R | 1994 | Biotechnology progress1994，10，5： | 1 |
| 15 | Influence of carbon and nitrogen on the creep properties of type 316 stainless steel at 873 K 显示文摘 | Mathew M D Sasikala G Bhanu Sankara Rao K | 1991 | Materials Science and Engineering A1991，148，2： | 1 |
| 16 | Improved dielectric and mechanical properties of polystyrene-hybrid silica sphere composite induced through bifunctionalization at the interface显示文摘 | Sasikala T S Nair B P Pavithran C | 2012 | Langmuir2012，28，25： | 1 |
| 17 | On the occurrence of acid mueopolysaceharides in the spermatophores of two marine prawns,Penaeus indicus( Miline Edwards) and Metapenaeus monoceros ( Fabrieius) ( Crus-tacean : Macmra) 显示文摘 | Sasikala S L Subramoniam T | 1987 | Exp Mar Bio Ecol1987，113，： | 1 |
| 18 | Evaluation of dynamic fracture toughness of cold worked 9Cr-lMo steel 显示文摘 | Sathyanarayanan S Sasikala G Ray S K | 2004 | International Journal of Pressure Vessels and Piping2004，81，5： | 1 |
| 19 | Electrodeposition and optical characterization of CdS thin film on ITO- coated glass 显示文摘 | Sasikala G hnasekaran R D Subramanian C | 1997 | Thin Solid Films1997，302，： | 1 |
| 20 | Simple methods to synthesize 2 pyridones: reactions of 2-aroyl-3,3-bis(alkylsulfanyi)acrylaldehydes and cyanoacetamide 显示文摘 | MATHEWS A ANABHA E R SASIKALA K A | 2008 | Tetrahedron2008，64，8： | 1 |
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