维普期刊资源整合服务平台（V6.5）

164篇 您的检索式：作者名="SECOMBES C"

选中清除导出查看参考文献查看引证文献引用追踪共9页首页上一页第1页下一页末页 /9 跳转

	题名	作者	年代	出处	被引量
1	虹鳟肿瘤坏死因子(TNFα)基因体外表达与纯化的研究显示文摘将虹鳟两种肿瘤坏死因子TNFα1和TNFα2基因的成熟肽编码区域 ,用带有BamHI和HindIII酶切位点的基因特异性引物进行PCR扩增。扩增片段用限制性内切酶消化并连接到pQE30表达载体上 ,连接产物转化到大肠杆菌JM10 9感受态细菌中。转化子经PCR筛选 ,质粒测序 ,完成了虹鳟两种TNFα基因重组子的构建。重组子经体外培养和诱导后 ,获得了高效表达的TNFα重组蛋白。高效表达的重组TNFα不受诱导剂IPTG的影响 ,并且由于重组子高效表达而形成了包涵体 ;重组蛋白产量约占菌体蛋白总量的 2 5 %— 30 %。应用Ni NTA和固定金属亲和层析 (IM PC)技术 ,在变性条件下获得了高度纯化的重组蛋白 ,纯化重组蛋白的产量约为 0 5— 1mg L。	蔡中华宋林生 ZOU Jun C J SECOMBES 董双林	2003	水生生物学报2003，27，6：	7
2	The role of phagocytes in the protective mechanism of fish显示文摘	Secombes C J Fletcher T C	1992	Annual Rev of Fish Diseases1992，5372，：	2
3	Fluid flow along a channel with an asymmetric oscillating constriction显示文摘	Stephanoff K D Pedley T J Lawrence C J Secomb T W	1983	Nature1983，305，20：	1
4	In vitro activation of rainbow trout macrophages stimulates inhibition of Renibacterium salmoninarum growth concomitant with augmented generation of respiratory burst products显示文摘	Hardie L J Ellis A E Secombes C J	1996	Dis Aquat Organ1996，25，：	1
5	A review of CpGs and their-relevance to aquaculture 显示文摘	Carrington A C Secombes C J	2006	Veterinary Immunology and Im-munopathology2006，112，3：	1
6	Molecular identification and expression analysis of tumor necrosis factor in channel catfish (Ictalurus punctatus) 显示文摘	Zou j Secombes C J Long S	2003	Dev Comp Immunol2003，27，10：	1
7	Expression Kinetics of interferon and interferon-induced genes in Atlan- tic salmon ( Salmo salar ) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia vi- rus 显示文摘	Mc Beath A J Snow M Secombes C J	2007	Fish Shellfish Immunol2007，22，3：	1
8	Detection of specific and constitutive antibody secreting cells in the gills, head kidney and peripheral blood leucocytes of dab (Limanda limanda)显示文摘	Davidson G A Lin S H Secombes C J	1997	Vet Immunol Immuno- pathol1997，58，34：	1
9	Chemokines显示文摘	Laing K J Secombes C J	2004	Dev Comp Immunol2004，28，5：	1
10	Expression kinetics of interferon and interferon-induced genes in Atlantic salmon (Salmo salar) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia virus 显示文摘	McBeath A J A Snow M Secombes C J	2007	Fish & Shellfish Immunology2007，22，3：	1
11	Molecular and expression analysis of an interferon-gamma-inducible guanylate-binding protein from rainbow trout (Oncorhynchus mykiss) 显示文摘	Robertsen B Zou J Secombes C	2006	Dev Comp Immunol2006，30，11：	1
12	Expression of immune response genes in rainbow trout skin induced by Gyrodactylus derjavini infections 显示文摘	Lindenstrom T Secombes C J Buchmann K	2004	Veterin Irnmunol Im- munopathol2004，97，34：	1
13	Modulation of MHC class Ⅱ expression in rainbow trout Oncorhynchus mykiss macrophages by TNFα and LPS显示文摘	Knight J Stet R J Secombes C J	1998	Fish and Shellfish Immunology1998，8，：	1
14	Type 1-interferon signaling in fish 显示文摘	Collet B Secombes C J	2009	Fish Shellfish Immunol2009，12，：	1
15	The promoter for the Interferon Regulatory Factor (IRF)-2 in the rainbow trout Oncorhynchus mykiss: cloning and reporter gene activity显示文摘	Collet B McDonald C Secombes C J	2003	Fish & Shellfish Immunol2003，15，5：	1
16	Chemokines 显示文摘	Laing K J Secombes C J	2004	Dev Comp Immunol2004，28，：	1
17	Cloning and analysis of expression of a gilthead sea bream ( Sparus aurata) Mx cDNA 显示文摘	Tafalla C Aranguren R Secombes C J	2004	Fish Shellfish Immunol2004，16，：	1
18	Route of immunization influences the Generation of antibody secreting cells in the gut of rainbow trout ( Orworhynchus mykiss) 显示文摘	Davidson G A EIIis A E Secombes C J	1993	Dev Comp Immunol1993，17，：	1
19	Chemokines显示文摘	Laing K J Secombes C J	2004	Dev Comp Immunol2004，，28：	1
20	Chemokines 显示文摘	Laing K J Secombes C J	2004	Dev Comp Immunol2004，28，：	1

返回顶部每页显示：

共9页首页上一页第1页下一页末页 /9 跳转

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

用户提示