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| 1 | Induction of apoptosis by arsenic trioxide and hydroxycamptothecin in gastric cancer cells in vitro显示文摘AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells(SGC-7901,MKN-45,MKN-28)withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The cytotoxicity of As2O3 and HCPTon gastric cancer cells was determined by MTTassay.Morphologic changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron microscopy.Apoptosis and cell cycle changes of gastric cancercells induced by HCPT and As2O3 were investigatedby TUNEL method and flow cytometry.RESULTS As2O3 and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer cells.The IC50ofAs2O3 on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,respectively.The IC50of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h treatment.After 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G0/G1 phase was observedby flow cytometry.The apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As2O3.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L HCPT.The apoptotic indicewere 7%-15% as assessed by TUNEL method.The effect of As2O3 on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G1 phase,and blocked G2/M phase.HCPT also showed a remarkable cell cyclespecificity,by inducing cell death and apoptosis inG1 phase and arrest of proliferation at S phase.CONCLUSION As2O3 and HCPT exhibitsignificant cytotoxicity on gastric cancer cells byinduction of apoptosis.As2O3 and HCPT mighthave a promising prospect in the treatment ofgastric cancer,which needs to be further studied. | Tu SP Zhong J Tan JH Jiang XH Qiao MM Wu YX Jiang SH | 2000 | World Journal of Gastroenterology2000，6，4： | 43 |
| 2 | The Responsivity of the Bacteriorhodopsin Langmuir-Blodgett Film Intermittent Light显示文摘TheResponsivityoftheBacteriorhodopsinLangmuir-BlodgettFilmIntermittentLight￥GuangYuWANG;JianHuaYANG;ZhiGuangZHANG;ShuHuangLIN... | Wang, GY Yang, JH Zhang, ZG Lin, SH Lu, T Li, BF Jiang, L | 1996 | Chinese Chemical Letters1996，7，12： | 12 |
| 3 | Isolation and Structure Elucidation of Autolytimycin, A New Compound Produced by Streptomyces Autolyticus JX-47显示文摘Autolytimycin 1 was isolated from the culture filtrate of Streptomyces autolyticus JX-47, together with two known compounds, lebstatin 2 and 17-O-demethyl-geldanamycin 3. These compounds showed the activities of anti-HSV-I. The structure of 1 was determined by spectral analysis. | Li, MG Wu, SH Zhao, LX Zhang, Q Li, WJ Cui, XL Xu, LH Wu, DG Jiang, CL | 2001 | Chinese Chemical Letters2001，12，10： | 10 |
| 4 | Seroepidemiology of Helicobacter pylori infection among asymptomatic Chinese children显示文摘INTRODUCTIONIncreasing data has demonstrated that Helicobacterpylori(H.pylori),a spiral gram negativebacterium,colonized in human stomach,can causetype B gastritis,is strongly associated withgastric and duodenal ulceration,and has beenimplicated in the causation of gastric carcinomaand mucosa-associated lymphoid tissue(MALT)lymphomas.It has been reported that there | Xu CD Chen SN Jiang SH Xu JY | 2000 | World Journal of Gastroenterology2000，6，5： | 6 |
| 5 | Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg显示文摘 | He XW Wang F Jiang L Li J Liu SK Xiao ZY Jin XQ Zhang YN He Y Li K Guo YJ Sun SH | 2005 | 第二军医大学学报2005，26，10： | 5 |
| 6 | An- ti-lnflammatory Effects of Electroacupuncture in the respiratory system of a symptomatic amyotrophic lateral sclerosis animal model 显示文摘 | Jiang JH Yang EJ Baek MG Kim SH Lee SM Choi SM | 2011 | Neuredegenerative Dis2011，8，6： | 1 |
| 7 | Alkaloids patterns in Huperzia and some related genera of Lycopodiaceae Sensu Lato occurring in China and their contribution to classification 显示文摘 | Ma XQ Jiang SH Zhu DY | 1998 | Biochem Syst Ecol1998，26，7： | 1 |
| 8 | Antioxidant properties of the extract and subfractions from old leaves of toona sinensis roem(meliaceae)显示文摘 | Jiang SH Wang C L Chen ZQ | 2009 | Journal of Food Biochemistry2009，33，3： | 1 |
| 9 | Induction of cytochrome P450 3A by Shexiang Baoxin pill and its main components 显示文摘 | Jiang B Cai F Gao SH | 2012 | Chem Biol Interact2012，195，2： | 1 |
| 10 | A comparison of steep and shallow needle trajectories in blind axillary vein puncture显示文摘 | Jiang M Gong XR Zhou SH | 2013 | Pacing Clin Electrophysiol2013，36，9： | 1 |
| 11 | Design and synthesis of novelbis(L-amino acid) ester prodrugs of 9-adenine (PMEA) with improved anti-HBV activity显示文摘 | Fu XZ Jiang SH Li C | 2007 | Bioorg Med Chem Lett2007，17，2： | 1 |
| 12 | Chemical constituents of Pteris multfida and their inhibitory effects on growth of rat prostatic epi- thelial cells in vitro 显示文摘 | Qin B Zhu DY Jiang SH | 2006 | Chin J Nat Med2006，4，6： | 1 |
| 13 | NF-κB pathway protects cochlear hair cells from aminoglycoside-induced ototoxicity显示文摘 | Jiang H Sha SH Schacht J | 2005 | J Neurosci Res2005，79，5： | 1 |
| 14 | NF-κB pathway protects cochlear hair cells from aminoglycoside-induced ototoxicity显示文摘 | Jiang H Sha SH Schacht J | 2005 | J Neurosci Res2005，79，5： | 1 |
| 15 | Machinery fault diagnosis using supervised manifold learning 显示文摘 | JIANG Q SH JIA M P HU H | 2009 | Mechanical Systems and Signal Processing2009，23，7： | 1 |
| 16 | Hydrogen sulfide protects soybean seedlings againstdrought-induced oxidative stress显示文摘 | ZHANG H JIAO H JIANG CX WANG SH WEIZ J LUO JP JONES R L | 2010 | Acta Physiologiae Plantarum2010，32，5： | 1 |
| 17 | A study and implementation on the data reduction based on the curvature of point clouds 显示文摘 | Jiang Hong Sun Wenlei Sh/Yongfang Huang mhua | 2014 | Computer Modeling and New Technologies2014，18，3： | 1 |
| 18 | Interaction of Pdcd4 witheIF4E inhibits the metastatic potential of hepatocellular carcinoma显示文摘 | Jiang Y Zhang SH Han GQ | 2010 | Biomed Pharmacother2010，64，6： | 1 |
| 19 | Ahernate arrangement of PpL B3 domain and SpA D domain creates synergistic double-site binding to VH3 and Vkappa regions of fab显示文摘 | Jiang SH Wang JF Xu R | 2008 | DNA Cell Biol2008，27，8： | 1 |
| 20 | A gap in the Indo Pacific warm pool over the South China Sea in boreal winter Seasonal development and interannual variability显示文摘 | Liu Q Y Jiang X Xie Sh P | 2004 | Journal of Geophysical Research2004，109，07： | 1 |
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