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| 1 | Effect of heparin on high glucose induced proliferation and expression of matrix metalloproteinases in normal human mesangial cells显示文摘Background The pathogenesis of diabetic nephropathy (DN) is a complex pathophysiological process.Its precise mechanism is not fully known. In recent years it has been recognized that synthesis of various extracelluar matrix (ECM) components may increase, and that degradation of ECM may decrease in DN. It was reported heparin could inhibit mesangial cells proliferation in vitro. The main aim of this study is to explore whether heparin inhibits proliferation of mesangial cells grown in high glucose concentration and to measure the effect of heparin on matrix metalloproteinases (MMPs) expression in mesangial cells. Methods The medium contained either low glucose (5 mmol/L) or high glucose (25 mmol/L). The concentrations of heparin in the culture medium were 0, 25, 50,100, 200 or 400 μg/mL. A metabolic (WST-1) assay was used to measure mesangial cell proliferation and Western blot analysis was used to measure MMPs expression of mesangial cells. Results Normal human mesangial cell (NHMC) proliferation was higher in high glucose (HG) medium than in low glucose (LG) medium. They showed a 1.93 fold expansion after 72 h in high glucose in contrast to a 1.63 fold expansion in low glucose. In the presence of heparin, mesangial cells proliferation was inhibited, which was more obvious at high glucose concentrations than at low glucose concentrations. In high glucose, with heparin concentration of 50, 100, 200 and 400 μg/mL, the mesangial cells showed a 0. 61 fold, 0.52 fold, 0.52 fold and 0.41 fold reductions in cell number compared to cells grown without heparin. In low glucose, only concentrations of 200 μg/mL and 400 μg/mL showed reduction in cell number, namely 0.54 fold and 0.45 fold, when compared to cells grown without heparin. In Western blot analysis,MMP1, MMP2, MMP3 and MMP9 was expressed by mesangial cells expressed in both high and low glucose concentrations, which was more prominent in high glucose medium. Incubation of heparin further increased expression of MMP1, MMP2, MMP3 and MMP9. Conclusions This study suggests that glucose can accelerate mesangial cell proliferation while heparin can reduce proliferation, being more obvious at high glucose concentrations. Higher glucose concentrations led to increased MMP expression, which may take part in the regulation of mesangial matrix synthesis and degradation. Addition of heparin resulted in a corresponding increase in MMP expression, most notably at high glucose concentrations, indicating a potentially renoprotective role in DN. | ZHOU Qiao-ling Yasumoto Yuichiro Tsukamoto Masatoshi Nozaki Tsuyoshi Sogabe Atsushi Harada Kouji ZHANG Yi-xiang LIN Xiao-yan ZHANG Yang-de Arima Terukatsu | 2005 | Journal of Central South University of Technology2005，12，z1： | 3 |
| 2 | A novel binder for the graphite anode of rechargeable lithium ion batteries for the improvement of reversible capacity显示文摘 | Sogabe T Kuroda K | 2001 | Carbon2001，39，： | 1 |
| 3 | Phase relations and dielectric properties in the Bi2O3-ZnO-Ta2O5 system显示文摘 | YOUN H J SOGABE T RANDALL C A | 2001 | J Am Ceram Soc2001，84，11： | 1 |
| 4 | An extension of the conjugate residual method to non-symmetric linear systems显示文摘 | SOGABE T SUGIHARA M ZHANG S L | 2009 | J Comput Appl Math2009，226，1： | 1 |
| 5 | A study of running safety and ride comfort of floating tracks for high- speed train显示文摘 | WATANABE T SOGABE M YAMAZAKI T | 2008 | Journal of Mechanical Systems for Transpor- tation and Logistics2008，1，1： | 1 |
| 6 | Water - Swellable Polyelectrolyte Mierogels Polymerized in an Inverse Microemulsion Using a Nonionic Surfactant 显示文摘 | Kaneda I Sogabe A Nakajima H | 2004 | Journal of Colloid and Interface Science2004，275，2： | 1 |
| 7 | Clinical analysis of leg ulcers and gangrene in rheumatoid arthritis显示文摘 | Hasegawa M Nagai Y Sogabe Y | 2013 | J Dermatol2013，40，12： | 1 |
| 8 | Morphology and X-Ray Diffraction Peak Widths of Aluminum Nitride Single Crystals Prepared by the Sublimation Method 显示文摘 | TANAKA M NAKAHATA S SOGABE K | 1997 | Jpn J Appl Phys1997，36，： | 1 |
| 9 | Improvement in properties and air oxidation resistanee of carbon materials by boron oxide impregnation 显示文摘 | Sogabe T Okada O Kuroda K | 1997 | Carbon1997，35，1： | 1 |
| 10 | Imatinib mesylate plus G-CSF therapy for chronic myelogenous leukemia in the blastic crisis显示文摘 | MORITA R HASHINO S SOGABE S | 2004 | Rinsho Ketsueki2004，45，： | 1 |
| 11 | Location and frequency of lesions in patients with IgG4-related ophthalmic diseases显示文摘 | Sogabe Y Ohshima K Azumi A | 2014 | Graefes Arch Clin Exp Ophthalmol2014，252，3： | 1 |
| 12 | The usefulness of infraorbital nerve enlargement on MRI imaging in clinical diagnosis of IgG4-related orbital disease显示文摘 | Ohshima K Sogabe Y Sato Y | 2012 | Jpn J Ophthalmol2012，56，4： | 1 |
| 13 | PhaseⅡstudy of combined treatment with irinotecan and S-1(IRIS)in patients with inoperable or recurrent advanced colorectal cancer(HGCSG0302)显示文摘 | Komatsu Y Yuki S Sogabe S | 2011 | Oncology2011，80，12： | 1 |
| 14 | A new family of k-Fibonaccinumbers显示文摘 | EL-MIKKAWY M SOGABE T | 2010 | Applied Mathematics and Computation2010，215，： | 1 |
| 15 | Vitamin K1(Phylloquinone)or vitamin K2(Menaquinone-4)induces intestinal alkaline phosphatase gene expression显示文摘 | Haraikawa M Sogabe N Tanabe R | 2011 | Journal of Nutritional Science and Vitaminology2011，57，4： | 1 |
| 16 | Improvement in properties and air oxidation resistance of carbon materials by boron oxide impregnation显示文摘 | Sogabe T Okada O Kuroda K | 1997 | Carbon1997，35，： | 1 |
| 17 | Phase relations and dielectric properties in the Bi2O3-ZnO-Ta2O5 system显示文摘 | Youn H J Sogabe T Randall C A | 2001 | J Am Ceram Soc2001，84，11： | 1 |
| 18 | Water-swellable polyelectrolyte microgels polymerized in an in-verse microemulsion using a nonionic surfactant显示文摘 | Isamu Kaneda Atsushi Sogabe Hideo Nakajima | 2004 | Jour-nal of Colloid and Interface Science2004，，275： | 1 |
| 19 | An extension of the conjugate residual method to nonsymmetric linear systems 显示文摘 | SOGABE T SUGIHARA M ZHANG S L | 2009 | J Comp Appl Math2009，226，1： | 1 |
| 20 | Structure-based approach for the discovery of Pyrrolo pyrimidine- based EGFR T790M/LSSSR mutant inhibitors 显示文摘 | Sogabe S Kawakita Y Igaki S | 2012 | ACS Med ChemLett2012，4，2： | 1 |
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