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| 1 | Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC. | Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard | 2012 | World Journal of Gastroenterology2012，18，26： | 12 |
| 2 | Inheritance of fresh seed dormancy in groundnut 显示文摘 | Asibuo J Y Akromah R Safo-Kantanka O Adu-Dupaah H Seth KO Agyemen A | 2008 | African Journal of Biotechnology2008，7，4： | 1 |
| 3 | Clinical pratiee guideline: Hoarseness(Dysphonia) 显示文摘 | Seth R Seth H Ellen S | 2009 | Otolaryngol Head Neck Surg2009，141，： | 1 |
| 4 | Efficacy of group psychotherapy to reduce depressive symptoms among HIV- infected individuals:a systematic review and meta-analysis 显示文摘 | SETH H DEBORAH R M GLORIA O | 2007 | AIDS PATIENT ACRE and STDs2007，21，10： | 1 |
| 5 | Optical sectioning of pulp fi- bers using confoeal scanning laser microscopy 显示文摘 | Jang H F Rohertson A G Seth R S | 1991 | Tappi Journal1991，74，10： | 1 |
| 6 | Rainwater harvesting agriculture in Gansu Province, People's of Republic of China显示文摘 | Seth Cook Li F R Wei H L | 2000 | Soil and Water Conservation2000，55，2： | 1 |
| 7 | Flood esti mation using a GIUH based on a conceptual rainfall2runoff model and GIS显示文摘 | JainSK Chowdhary H Seth SM Ne ma R K | 1997 | ITC journal1997，1，： | 1 |
| 8 | Using confocal microscopy to characterize the collapse behavior of fiber显示文摘 | Jang H F Seth R S | 1998 | Tappi J1998，81，5： | 1 |
| 9 | A study of ethyl glueuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol显示文摘 | Hφseth G Karinen R Christophersen AS et ol | 2007 | Foren-ic Sci Int2007，165，1： | 1 |
| 10 | Transverse dimensions of wood pulp fibres by confocal laser scanning microscopy and image analysis显示文摘 | Jang H F Robertson A G Seth R S | 1992 | Journal of Materials Science1992，27，23： | 1 |
| 11 | Efficiency and risk in Japanese banking 显示文摘 | Altunbas Y Liu M H Molyneux P Seth R | 2000 | Journal of Banking and Finance2000，24，： | 1 |
| 12 | Prevention of renal ischemia-reperfusion-induced injury in rats by picroliv显示文摘 | Seth P Kumari R Madhavan S Singh AK Mani H Banaudha KK | 2000 | Biochem Phamacol2000，59，10： | 1 |
| 13 | Perylene-3,4,9,10-tetracarboxylic acid diimides: Synthesis, Physical properties, and use in organic electronics显示文摘 | Chun H Stephen B Seth R M | 2011 | J Org Chem2011，76，： | 1 |
| 14 | Rainwater harvesting agriculture in Gansu Province, People's of Republic of China显示文摘 | Seth Cook Li F R Wei H L | 2000 | Soil and Water Conservation2000，55，2： | 1 |
| 15 | A pharmacokinetic study of ethyl glucuronide in blood and urine: applications to forensic toxicology显示文摘 | Hφseth G Bernard JP Karinen R | 2007 | Forensic Sci Int2007，172，23： | 1 |
| 16 | Disappearance of ethyl glucuronide during heavy putrefaction显示文摘 | Hφseth G Karinen R Johnsen L | 2008 | Forensic Sci Int2008，176，23： | 1 |
| 17 | SuomiNet:A real-time national GPS network for atmospheric research and education 显示文摘 | Ware R H David W F Seth A S | 2000 | Bull Amer Meteorol Soc2000，81，4： | 1 |
| 18 | Efficiency and risk in japanese banking 显示文摘 | Altunbas Y Liu M H Molyneux P Seth R | 2000 | Journal of Banking & Finance2000，24，10： | 1 |
| 19 | Reduction in cutaneous and hepatic glutathione contents,glutathionr S-transferase and aryl hydrocarbonhydroxylase activities following topical application of acrylamide to mouse显示文摘 | Mukhtar H Dixit R Seth P K | 1981 | Toxicol Lett1981，9，： | 1 |
| 20 | Using microscopy to characterize the collapse behavior of fibers显示文摘 | Jang H F Seth R S | | 0，，05： | 1 |
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