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| 1 | Construction and characterization of an HCV-derived multi-epitope peptide antigen containing B-cell HVR1 mimotopes and T-cell conserved epitopes显示文摘Hepatitis C(HCV) genome is highly variable,particularly in the hypervariable region 1 (HVR1) of its E2 envelope gene.The variability of HCV genome has been a major obstacle for de-veloping HCV vaccines.Due to B-cell HVR1 mimotopes mimicking the antigenicity of natural HVR1 epitopes and some T-cell epitopes from the consensus sequence of HCV genes conserving among the different HCV genotypes,we synthesized an minigene of HCV-derived multi-epitope peptide an-tigen(CMEP) ,which contains 9 B-cell HVR1 mimotopes in E2,2 conserved CTL epitopes in C,1 conserved CTL epitope in NS3 and 1 conserved Th epitope in NS3.This minigene was cloned into a GST expression vector to generate a fusion protein GST-CMEP.The immunogenic properties of CEMP were characterized by HCV infected patients' sera,and found that the reactivity frequency reached 75%.The cross reactivity of anti-CEMP antibody with different natural HVR1 variants was up to 90%.Meanwhile,we constructed an HCV DNA vaccine candidate,plasmid pVAX1.0-st-CMEP carrying the recombinant gene(st) of a secretion signal peptide and PADRE universal Th cell epitope sequence in front of the CMEP minigene.Immunization of rabbits with pVAX1.0-st-CMEP resulted in the production of antibody,which was of the same cross reactivity as the fusion protein GST-CMEP.Our findings indicate that the HCV-derived multi-epitope peptide antigen in some degree possessed the characteristics of neutralizing HCV epitopes,and would be of the value as a candidate for the development of HCV vaccines. | GAO Jun1,2,GONG Yuping1,ZHAO Ping1,ZHU Qing3,YANG Xiaoping1 & QI Zhongtian1 1.Department of Microbiology,Stake Key Laboratory of Medical Immunology,Second Military Medical University,Shanghai 200433,China 2.Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China 3.Department of Epidemiology,Naval Medical Research Institute,Shanghai 200433,China | 2006 | Science China(Life Sciences)2006，49，5： | 7 |
| 2 | Glomerular filtration rate esti- mated by X ray fluorescence technique in children comparison between the plasma disappearance of 99mTcDTPA and iohexol after urography 显示文摘 | Stake G Monn E Rootweh K | 1990 | Scand J Clin Lab Inv1990，50，2： | 1 |
| 3 | Microstructure and Particle laden Flow in Diesel Particulate Filter显示文摘 | Yamam 0 K Stake S Yamashit H | 2009 | Inter- national Journal of Thermal Sciences2009，48，2： | 1 |
| 4 | The informa- tional relevance of corporate social responsibility:evidence from DS400 index reconstitutions 显示文摘 | Ramchander S Schwebach R G Staking K I M | 2012 | Strategic Management Journal2012，33，3： | 1 |
| 5 | Effect of quench rate,pre-age stretch and artificial aging on the Al-Li-Cu-X alloy AF/C458显示文摘 | CSONTOS A A GABLE B M STAKE J E A | 2004 | Light Metals edited by Tabereaux A T TMS (the minearal metals and material society)2004，，： | 1 |
| 6 | A quench sensitivity study on the novel Al-Li-Cu-X alloy F/C458显示文摘 | GABLE B M CSONTOS A A STAKE J E A | 2002 | Journal of Light Metals2002，2，： | 1 |
| 7 | A single plasma sample method for estimation of the glomerular rate in infants andchildren using iohxol, establishment of the optimal plasma sampling time and a comparison with the 99mTc-DTPA meth- od 显示文摘 | Stake G Monn E Rootwelt K | 1991 | Seand J Clin Lab Inv1991，51，4： | 1 |
| 8 | Disaster training for pre - hospital providers显示文摘 | Chaput CJ Deluhery MR Stake CE | 2007 | Pre - hospital Emergency Care2007，11，4： | 1 |
| 9 | Temporal and spatial archaeal colonization of hydrothermal vent deposits 显示文摘 | Page A Tivey M K Stakes D S | 2008 | Environmental Microbiology2008，10，： | 1 |
| 10 | Cold-seeps and authigenic carbonate formation in Monterey Bay, California 显示文摘 | STAKES D S ORANG D PADYAB J B | 1999 | Marine Geology1999，159，14： | 1 |
| 11 | Influence of platelet count on the expression of platelet collagen receptor glycoprotein Ⅵ (GP Ⅵ) in patients with acute coronary syndrome显示文摘 | Bigalke B Stellos K Stakes D | 2009 | Thromb Haemost2009，101，5： | 1 |
| 12 | Cold-seeps and authigenic carbonate formation in Monterey Bay, California 显示文摘 | Stakes D S Orange D Paduan J B | 1999 | Marine Geology1999，159，： | 1 |
| 13 | Contrast enhanced MR imaging of liver and spleen:first experience in human with a new superparamagnetic iron oxide显示文摘 | Hamm B Stakes T Taupitz M | 1994 | Magn Reson Imaging1994，4，5： | 1 |
| 14 | Evaluation of dietary zinc,cadmium,tin,lead,bismuth and arsenic toxicity in hens显示文摘 | HERMAYER K L STAKE P E SHIPPER L | 1977 | Poultry Science1977，56，5： | 1 |
| 15 | The Countenance of educational evaluation显示文摘 | Stake R E | 1967 | Teachers College Record1967，，68： | 1 |
| 16 | Optimal competition and allocation of space in shopping centers 显示文摘 | MECELI T J SIRMANS C F STAKE D | 1998 | Journal of Real Estate Research1998，16，1： | 1 |
| 17 | Mineralogy and stable isotope geochemistry of hydrothermally altered oceanic rocks显示文摘 | Stakes D S O'Neil J tk | 1982 | Earth and Planetary Science Letter1982，57，： | 1 |
| 18 | Biogeological mineralization in deep-sea hydrothermal deposits显示文摘 | COOK T L STAKES D S | 1995 | Science1995，267，： | 1 |
| 19 | Qualitative research and case study 显示文摘 | Stake RE | 2013 | Silpakom Educational Research Journal2013，3，12： | 1 |
| 20 | Evaluation of dietary zinc,cadmium,tin,lead,bismuth and arsenic toxicity in hens显示文摘 | HERMAYER K L STAKE P E SHIPPER L | 1977 | Poultry Science1977，56，5： | 1 |
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