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| 1 | Expression of the oxygen-sensitive transcription factor subunit HIF-1α in patients suffering from secondary Raynaud syndrome显示文摘Anti-ischemic therapy remains a challenge due to the complexity of hypoxia response pathways. Hypoxia-inducible factor (HIF)-1 is a heterodimer tran scription factor con sisti ng of 2 sub units, HIF-1α and HIF-1β. Hypoxia-depe ndent activatio n of HIF-1α regulates cellular 02 homeostasis. Raynaud syndrome (RS), as a comorbidity of the autoimmune disease systemic sclerosis (SS), is characterized by vasospasms that limit blood flow to the limbs, resulting in hypoxia. A single-center randomized study was con ducted to compare prostagla ndin E1 (PgEI) therapy with a treatme nt combi ning PgE1 and an en dotheli n-1 blocker, bosentan. A total of 30 patients suffering from SS with RS were enrolled. We examined the regulation of HIF-1α, its target heme oxygenase-1 (HMOX-1), and the serum levels of the HIF-1α protein in a subset of patients as well as in ten healthy individuals. The expression of HIF-1α and HMOX-1 in monocytes was measured using absolute plasmid-based quantitative real-time PCR, whereas serum HIF-1α levels were measured with ELISA. Samples were taken at the time of randomization and after 24 weeks. We found that HIF-1α and HMOX-1 mRNA expression in monocytes and serum HIF-1α protein levels were significantly higher in the SS/RS patients compared to the healthy control group. Single-drug therapy significantly increased HIF-1α and HMOX-1 mRNA expression in monocytes and serum HIF-1α protein levels in the SS/RS patients compared to those at the time of randomization, whereas combining PgE1 with an en dotheli n-1 blocker preve nted the further in creases in HIF-1α and HMOX-1 expressi on. We propose HIF-1α and HMOX-1 as novel markers for anti-ischemic therapy in RS. | Lukas Andreas Heger Mark Kerber Marcus Hortmann Samuel Robinson Maximilian Mauler Daniela Stallmann Daniel Duerschmied Christoph Bode Christoph Hehrlein Ingo Ahrens | 2019 | Acta Pharmacologica Sinica2019，40，4： | 5 |
| 2 | Chip-based digital PCR as a novel detection method for quantifying microRNAs in acute myocardial infarction patients显示文摘miRNAs 为尖锐心肌的梗塞(AMI ) 作为潜在的 biomarkers 显示出诺言。然而,当前的使用的量的即时 PCR (qRT-PCR ) 完全为 nucleic 酸的相对表示允许,它产生日常可变性,它限制了把 miRNAs 用作 biomarkers 的有效性。在这研究,我们探索了技术质量和一种新技术的诊断潜力,基于薄片的数字 PCR,在确定在有 AMI 和 ischaemia-reperfusion 损害(I/R ) 的病人的 miRNAs。在合成 C.elegans-miR-39 的一个冲淡系列,基于薄片的数字 PCR 与 qRT-PCR 相比显示了变化(8.9% 对 46.3%) 和察觉(0.2 copies/L 对 1.1 copies/L ) 的更低的限制的一个更低的系数。在从有圣举起心肌的梗塞( STEMI )的 24 个病人和有稳定的冠的动脉疾病( CAD )的 20 个病人镇定的浆液在经皮的冠的干预(一种总线标准)以后的病人,我们使用了 qRT-PCR 和多路的基于薄片的数字 PCR 确定他们在优先的研究在 AMI 被验证了的 miRNA-21 和 miRNA-499 的浆液层次。在 STEMI, I/R 损害经由圣片断分辨率(ST-R ) 的测量被估计。基于薄片的数字 PCR 处于在稳定的 CAD 和 STEMI 组之间的 miR-21 层次的差别揭示了统计意义(118.8 copies/L 对 59 copies/L;P=0.0300 ) ,而 qRT-PCR 是不能的到达意义(136.4 copies/L 对 122.8 copies/L;P=0.2273 ) 。为 miR-499 层次,基于薄片的数字 PCR 和 qRT-PCR 揭示了稳定的 CAD 和 STEMI 组之间的统计上重要的差别(2 copies/L 对 8.5 copies/L, P=0.0011;0 copies/L 对 19.4 copies/L;P < 0.0001 ) 。在 miR-21/499 层次和 ST-R 之间没有协会一种总线标准以后。我们的结果证明基于薄片的数字 PCR 展出优异技术质量和诺言是一个优异方法因为确定的 miRNA 在发行量铺平,它可以为直接确定成为一个更精确、可再现的方法 miRNAs,特别地为在大多中心的使用临床的试用。 | Samuel ROBINSON Marie FOLLO David HAENE Maximilian MAULER Daniela STALLMANN Lukas Andreas HEGER Thomas HELBING Daniel DUERSCHMIED Karlheinz PETER Christoph BODE Ingo AHRENS Marcus HORTMANN | 2018 | Acta Pharmacologica Sinica2018，39，7： | 5 |
| 3 | Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11-or gentamicin-containing calcium phosphate cement显示文摘 | Stallmann H P Faber C Bronckers A L | 2004 | J Antimicrob Chemother2004，54，2： | 1 |
| 4 | Automatic Texture Mapping of Architectural and Archaeological 3D Models显示文摘 | Kersten T P Stallmann D | 2012 | ISPRS-International Archives of the Photogrammetry Remote Sensing and Spatial Information Sciences2012，5，： | 1 |
| 5 | System Calibration for Direct Georeferencing 显示文摘 | Cramer M Stallmann D | 2002 | International Archives of Photogrammetric and Remote Sensing2002，34，3： | 1 |
| 6 | On the use of GPS/Inertial exterior orientation parameters in airborne photogrammetry显示文摘 | Michael Cramer Dirk Stallmann | 2001 | OEEPE-Workshop Integrated Sensor Orientation2001，，： | 1 |
| 7 | In vitro gentamicin release from commercially available calciumphosphate bone substitutes influence of carrier type on duration of the release profile 显示文摘 | Stallmann HP Faber C Bronckers AL | 2006 | BMC Musculoskelet Disord2006，7，： | 1 |
| 8 | Osteomyelitis prevention in rabbits using antimicrohial peptide or gentamiein-containing calcium phosphate cement 显示文摘 | Stallmann HP Faber C Bronckers AL | 2004 | J Antimierob Chemother2004，2，： | 1 |
| 9 | On the Performance of Digital Airboren Pushbroom Cameras for Photogrammetric Data Processing - A Case Study显示文摘 | HAALA N STALLMANN D CRAMER M | 2000 | IAPRS2000，33，： | 1 |
| 10 | In vitro gentamicin release from commercially available calcium-phosphate bone substitutes influence of carrier type on duration of the release profile 显示文摘 | Stallmann HP Fabor C Bronckers Al | 2006 | BMC Musculoskelet Disorders2006，26，： | 1 |
| 11 | Osteomyelitis prevention in rabbits using antimicrobial peptide hLFl-11-or gentamicincontaining calcium phosphate cement显示文摘 | Stallmann HP Faber C Bronckers AL | 2004 | J Antimicrob Chemother2004，54，2： | 1 |
| 12 | Direct Georeferencing Using GPS/Inertial Exterior Orientations for Photogrammetric Applications显示文摘 | CRAMER M STALLMANN D HAALA N | 2000 | International Archives of Photogrammetry and Remote Sensing2000，33，3： | 1 |
| 13 | Direct georeferencing u- sing GPS/Inertial exterior orientations for photogrammetric applications 显示文摘 | Cramer M Stallmann D Haala N | 2000 | International Archives of Photogrammetry and Remote Sensing2000，33，3： | 1 |
| 14 | Direct georeferencing using GPS/ inertial exterior orientations for photogrammetrie applieations显示文摘 | Cramer M Stallmann D Haala N | 2000 | International Archives of Photogrammetry and Remote Sensing2000，33，3： | 1 |
| 15 | Direct Georeferencing Using GPS/Inertial Exterior Orientations for Photogrammetric Applications 显示文摘 | Cramer M Stallmann D Haala N | 2000 | International Archives of Photogrammetry and Remote Sensing2000，33，3： | 1 |
| 16 | System Calibration for Direct Georeferencing 显示文摘 | CRAMER M STALLMANN D | 2002 | International Archives of Photogrammetry Remote Sensing and Spatial Information Sciences2002，34，3： | 1 |
| 17 | Osteomyelitis pre- vention in rabbits using antimicrobial peptide hLFl-11-or gen- tamicin-containing calcium phosphate cement显示文摘 | Stallmann HP Faber C Bronckers AL el al | 2004 | J Antimicrob Chemother2004，54，2： | 1 |
| 18 | Advancement in Matching of SPOT Images by Integration of Sensor Geometry and Treatment of Radiometric Differences显示文摘 | Baltsavias E Stallmann D | 1992 | IAPRS1992，29，4： | 1 |
| 19 | Untersuchung zur Abscheidung von Nickel-Bor Legierungsschichten (Study of the Deposition of Nickel-Boron Alloy Layers)显示文摘 | Stallmann K Speckhardt H | | Metall0，39，3： | 1 |
| 20 | System Calibration for Di- rect Georeferencing 显示文摘 | Cramer M Stallmann D | 2002 | International Archives of Photogrammetry Remote Sensing and Spatial In- formation Sciences2002，34，3： | 1 |
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