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| 1 | 巨峰和无核早红葡萄在越南谅山试种观察显示文摘[目的]观察巨峰、无核早红葡萄在越南谅山省的引种表现,为其在越南推广种植提供参考依据.[方法]2009年春从中国南宁引入巨峰、无核早红两个鲜食葡萄品种在越南谅山省试种,调查其枝蔓生长量、物候期、果实性状、产量等相关指标.[结果]至第1年抚育冬季,巨峰葡萄枝蔓总长234.5 cm,上架茎粗0.84 cm;无核早红葡萄枝蔓总长294.1 cm,上架茎粗0.86 cm.在物候期方面,巨峰葡萄从萌芽至果实成熟需141~151 d,表现中熟;无核早红从萌芽至果实成熟需142~151 d,未表现其应有的早熟特性.在果实性状方面,巨峰葡萄2010~2012年3年平均单果重8.9 g,种子数1.04粒,可溶性固形物(TSS)含量17.5%;无核早红葡萄2010~2012年3年平均单果重6.8 g,TSS含量13.9%.在产量方面,巨峰葡萄2010~2012年产量分别为13.69、17.50和16.24 t/ha;无核早红葡萄2010~2012年产量分别为10.81、16.02和20.92t/ha.[结论]巨峰葡萄在越南谅山省种植表现丰产稳产优质,具有较好的适栽性,可大面积推广种植;无核早红葡萄表现丰产稳产,但表现低糖低酸,极易裂果,着色困难,不宜在越南大面积发展. | 黄江流 PHAM Thi Thang 罗瑞鸿 NGUYN Thi Hà 吕荣华 LUONG Dǎng Ning CHU Vǎn DUòng NGUYN Manh Tòng 彭宏祥 Ly Ming Nguyet LU'O'NG i Thuy CHU Xun Tien VI Vǎn Dúc NGUYN Duy Dng Ly Dúc Khiêm 熊俏 | 2014 | 南方农业学报2014，45，7： | 5 |
| 2 | Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype. | C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf | 2014 | World Journal of Cardiology2014，6，4： | 3 |
| 3 | Multivariate Statistical Modeling for Image Denoising Using Wavelet Transforms显示文摘 | Dongwook Cho Tien D Bui | 2005 | Signal Processing:Image Communication2005，20，： | 1 |
| 4 | Balloon dilation of the nasolacrimal duct显示文摘 | Tien DR Young D | 2005 | J AAPOS2005，9，5： | 1 |
| 5 | Oxidation of persistent environmental pollutants by a white rot fungus 显示文摘 | Bumpus J A Tien M Wright D | 1985 | Science1985，228，4706： | 1 |
| 6 | Temporal variability in dissolved phosphorus concentrations in the subtropical North Pacific Ocean显示文摘 | Karl D M Tien G | 1997 | Marine Chemistry1997，56，： | 1 |
| 7 | Solving Stiff Differential Equation in the Simulation of Physical Systems显示文摘 | Tien D Bui | 1990 | Simulation1990，54，5： | 1 |
| 8 | Oxidation of persistent environmental pollutants by white rot fungus显示文摘 | Bumpus JA Tien M Wright D | | 0，，： | 1 |
| 9 | Oxidation of persistent environmental pollutant by a white rot fungus显示文摘 | Tien M Wright D | 1985 | Science1985，228，： | 1 |
| 10 | Multivariate statistical modeling for image denoising using wavelet transforms显示文摘 | Dongwook Cho Tien D Bui | 2005 | Signal Processing:Image Communication2005，20，： | 1 |
| 11 | Altered immunity accompanies disease progresssion in a mouse model of prostate dysplasia显示文摘 | Tien A H Xu L Helgason C D | 2005 | Cancer Res2005，65，7： | 1 |
| 12 | Fr-ontoethmoidal giant cell reparative granuloma显示文摘 | FELSBERG G J TIEN R D MCLENDON R E | 1995 | AJNR Am J Neuroradiol1995，16，： | 1 |
| 13 | Formation of perfused, functional microvascular tubes in vitro 显示文摘 | Chrobak K M Potter D R Tien J | 2006 | Microvasc Res2006，71，3： | 1 |
| 14 | Invariant Fourier Wavelet Descriptor for Pattern Reeognition显示文摘 | Chen Guangyi Tien D B | 1999 | Pattern Recognition1999，32，7： | 1 |
| 15 | MR fat suppression combined with Gd-DTPA enhancement in optic neuritis and perineuritis显示文摘 | TIEN R D OLESEN J SIMON J H | 1991 | J Comput Assist Tomogr1991，15，： | 1 |
| 16 | ZnO nanowire growth and devices 显示文摘 | Heo Y W Norton D P Tien L C | 2004 | Materials Science Engineering2004，47，： | 1 |
| 17 | Expression, purification, and characterization of recombinant cyanovirin-N for vaginal anti-HIV microbicide development显示文摘 | Colleluori DM Tien D Kang E | 2005 | Protein Expr Purif2005，39，2： | 1 |
| 18 | Oxidation of per- sistent environmental pollutants by a white rot fungus显示文摘 | BUMPUS J A TIEN M WRIGHT D | 1985 | Sci- ence1985，228，4706： | 1 |
| 19 | MR imaging of pineal tumors显示文摘 | Robert D Tien AJ Barkovich MSB | 1990 | AJR1990，155，： | 1 |
| 20 | Chorea-ballismus with nonketotic hyperglycemia in primary diabetes mellitus显示文摘 | Lai P H Tien R D Chang M H | 1996 | AJNR1996，17，6： | 1 |
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