|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production显示文摘L-Serine plays a critical role as a building block for cell growth, and thus it is difficult to achieve the direct fermentation of L-serine from glucose. In this study, Corynebacterium glutamicum ATCC 13032 was engineered de novo by blocking and attenuating the conversion of L-serine to pyruvate and glycine, releasing the feedback inhibition by L-serine to 3-phosphoglycerate dehydrogenase (PGDH), in combination with the co-expression of 3-phosphoglycerate kinase (PGK) and feedback-resistant PGDH (PGDHr). The resulting strain, SER-8, exhibited a lower specific growth rate and significant differences in L-serine levels from Phase I to Phase V as determined for fed-batch fermentation. The intracellular L-serine pool reached (14.22±1.41) μmol gCDM 1, which was higher than glycine pool, contrary to fermentation with the wild-type strain. Furthermore, metabolic flux analysis demonstrated that the over-expression of PGK directed the flux of the pentose phosphate pathway (PPP) towards the glycolysis pathway (EMP), and the expression of PGDHr improved the L-serine biosynthesis pathway. In addition, the flux from L-serine to glycine dropped by 24%, indicating that the deletion of the activator GlyR resulted in down-regulation of serine hydroxymethyltransferase (SHMT) expression. Taken together, our findings imply that L-serine pool management is fundamental for sustaining the viability of C. glutamicum, and improvement of C1 units generation by introducing the glycine cleavage system (GCV) to degrade the excessive glycine is a promising target for L-serine production in C. glutamicum. | LAI ShuJuan ZHANG Yun LIU ShuWen LIANG Yong SHANG XiuLing CHAI Xin WEN TingYi | 2012 | Science China(Life Sciences)2012，55，4： | 15 |
| 2 | Oil accumulation related to migration of source kitchens in the Lukeqin structural belt, Turpan-Hami Basin, China显示文摘The Lukeqin structural belt is the main heavy oil accumulation zone in the Turpan-Hami Basin. The recent discovery of light oil in the Triassic indicates that there may be multiple source kitchens contributing to the oil accumulation. According to oil geochemical analysis and oil-source correlation, the oil in deep and shallow reservoirs of the Lukeqin Oilfield presents different physical and saturated hydrocarbon mass spectrum characteristics. The Triassic heavy oil is from the northern Upper Permian lacustrine source rocks, and the light oil represented by the Yudong-9 Well is from the northwestern Lower Jurassic coal-measure source rocks. The timing of oil charging was determined by K/Ar isotope dating, reservoir fluid inclusion analysis and the evolution history of different source rocks. In summary, the accumulation process consists of two stages. From the end of Triassic to early Jurassic, the northern Permian source kitchen generated a considerable amount of oil, which was finally degraded to heavy oil, migrated to the south and then accumulated. The northwestern Jurassic coal-measure source kitchen began to generate oil at the end of Cretaceous, while the northern source kitchen could only generate a little hydrocarbon. The heavy oil and the light oil have different source rock locations, migration directions and accumulation times. The migration of hydrocarbon source kitchens affects the distribution of heavy oil and light oil reservoirs at the present time. | Liu Bo Huang Zhilong Tu Xiaoxian Sang Tingyi Chen Xuan | 2010 | Petroleum Science2010，7，3： | 2 |
| 3 | Trace elements in tree rings and their environmental effects: A case study in Xi’an City显示文摘Using wet digested method and ICP mass spectrometer, we analyzed the concentration of five trace elements (Cd, Mn, P, Zn and Pb) for the tree rings from both urban and suburbs of Xi'an. At the urban sampling site, one Chinese mahogany (Toona sinensis) disc and one phoenix tree (Firmiana simplex) disc were sampled from a steelworks in Xi'an City. At the suburb site, a Chinese mahogany disc was collected from a village in the south of the City. In addition, some soils near the roots of the sampled trees were collected. The analysis results indicate that the concentration of each of the five elements in annual rings has a positive correlation with the production of the steelworks. Statistical calculations show that Pb and Mn elements were stable without lag effects. That means these two elements do not move between rings. Cd displayed one year moving, P two years and Zn three years. These results are quite similar to those found by other methods. | LIU Yu TA WeiYuan BAO TingYi YANG ZengYue SONG HuiMing LIU Na WANG WeiPing ZHANG HongYi ZHANG Wei AN ZhiSheng | 2009 | Science China Earth Sciences2009，52，4： | 2 |
| 4 | Adsorption of bisphenol-A from aqueous solution onto minerals and carbon adsorbents显示文摘 | Tsai Wentien Lai Chiwei Su Tingyi | 2006 | Journd of Hazardous Materiols2006，134，13： | 1 |
| 5 | A(t,n)Multi-secret Sharing Scheme显示文摘 | Yang Chouchen Chang Tingyi Hwang Min-Shiang | 2004 | Applied Mathematics and Computation2004，151，2： | 1 |
| 6 | A (t, n) Multisecret Sharing Scheme显示文摘 | Yang Chouchen Chang Tingyi Hwang Min-Shiang | 2004 | Applied Mathematics and Computation2004，151，2： | 1 |
| 7 | Prospective study of ep- idermal growth factor receptor tyrosine kinase inhibitors concurrent with individualized radiotherapy for patients with locally advanced or metastatic Non-Small-Cell lung cancer显示文摘 | Wang Jing Xia Tingyi Wang Yingjie | 2011 | Int J Radiat Oncol Bi- ol Phys2011，81，3： | 1 |
| 8 | Promising clinical outcome of stereotactic body radiation therapy for patients with inoperable stage I/II non-small-cell lung cancer显示文摘 | Hongqi Li Qingxuan Sun | 2006 | Int J Radiat Oncol Biol Phys2006，66，1： | 1 |
| 9 | Entrapment of nanoscale zero-valent iron in chitosan beads for hexavalent chromium removal from wastewater显示文摘 | Liu Tingyi Zhao Lin Sun Desheng | 2010 | Journal of Hazardous Materials2010，184，123： | 1 |
| 10 | Discovery of Neoproterozoic Rocks in the Dunhuang Block: Clues to Correlation with Rodinia显示文摘Objective Rodinia was a supercontinent that comprised nearly all the current existing continents/blocks on Earth to form a coherent large landmass during the Meso-Neoproterozoic. Thus, discovery of Meso-Neoproterozoic rocks in a certain Precambrian block can provide important clues for studying the assembly and break-up history of Rodinia. | WANG Tingyi DIWU Chunrong ZHAO Jian GAN Baoping | 2020 | Acta Geologica Sinica(English Edition)2020，94，4： | 1 |
| 11 | A threshold signature scheme for group communications without a shared distribution center 显示文摘 | Chang Tingyi Yang Chouchen Hwang Minshiang | 2004 | Future Generation Computer Systems2004，20，6： | 1 |
| 12 | Simplified molecular gas film lubrication equation and accommodation coefficient effieients显示文摘 | SHI Baojun JI Jiadong YANG Tingyi | 2011 | Microsystem Technologies2011，17，： | 1 |
| 13 | Novel compact net-type resonators and their applications to microstrip bandpass filters显示文摘 | ChiFeng Chen TingYi Huang RueyBeei Wu | 2006 | IEEE Transactions on Microwave Theory and Techiques2006，54，1： | 1 |
| 14 | A multi-secret sharing scheme显示文摘 | Chang TingYi Hwang MinShiang | 2004 | Applied Mathematics and Computa-tion2004，151，2： | 1 |
| 15 | Promising clinical outcome of stereotactic body radiation therapy for patients with inoperable stage I/II Non-small Cell Lung Cancer显示文摘 | Tingyi Xia HongQi Li QingXuan Sun | 2006 | Int J Radiat Oncol Biol Phys2006，66，1： | 1 |
| 16 | A threshold signature scheme for group communications without a shared distribution center显示文摘 | Chang Tingyi Yang Chouchen Hwang Minshiang | 2004 | Future Generation Computer Systems2004，20，6： | 1 |
| 17 | A communication efficient three-party password authenticated key exchange protocol 显示文摘 | Chang Tingyi Hwang Minshiang Yang Weipang | 2011 | Information Sciences2011，181，1： | 1 |
| 18 | A threshold signature scheme for group communications without a shared center显示文摘 | Chang Tingyi Yang Chouchen Huang Minshiang | 2004 | Future Generation Computer Systems2004，20，6： | 1 |
| 19 | A (t, n) Multisecret Sharing Scheme显示文摘 | Yang Chouchen Chang Tingyi Hwang Minshiang | 2004 | Applied Mathematics and Computation2004，151，2： | 1 |
| 20 | A(t,n)Multi-secret Sharing Scheme显示文摘 | Yang Chouchen Chang Tingyi Hwang Miashiang | 2004 | Applied Mathematics and Computation2004，151，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员