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| 1 | 基因序列比对法验证实时荧光定量PCR阳性结果真伪显示文摘实时荧光定量PCR(q PCR)是检测食源性病毒的常用方法,其高度的灵敏性导致检测过程中容易产生假阳性结果。基因序列比对法是比利时根特大学食品微生物与食品保藏实验室新近建立的验证q PCR阳性结果真伪的一种方法。本研究以检测贝类诺如病毒GII.4(No V GII.4)为例,采用基因序列比对法验证q PCR阳性结果的真伪。在q PCR检测时添加102copies/m L的No V GII.4质粒作为标准阳性对照,旨在排除由于q PCR的高灵敏性而产生的假阳性结果。研究结果表明,在8份No V阳性的贝类样品中,2份为No V GII.4真阳性,2份可能为No V GII.4的变异株,1份为受到质粒污染的假阳性,其余3份为引物二聚体所致假阳性。基因序列比对法作为一种验证q PCR阳性结果真伪的高效、可行的方法,值得推广。 | 武娟 曹斌斌 李丹 张祺 左涛 赵玉然 Uyttendaele Mieke 薛长湖 唐庆娟 | 2016 | 中国食品学报2016，16，4： | 2 |
| 2 | Simulation modelling and risk assessment as tools to identify the impact of climate change on microbiological food safety – The case study of fresh produce supply chain显示文摘 | L. Jacxsens P.A. Luning J.G.A.J. van der Vorst F. Devlieghere R. Leemans M. Uyttendaele | 2009 | Food Research International2009，，7： | 2 |
| 3 | Characterization of growth and metabolite production of Lactobacillus reuteri during glucose/glycerol cofermentation in batch and continuous cultures显示文摘 | El-Ziney M G Arneborg N Uyttendaele M | 1998 | Biotechnology Letters1998，20，： | 1 |
| 4 | Dynamics of boar semen motility inhibition as a semi-quantitative measurement of Bacillus cereus emetic toxin (Cereulide)显示文摘 | RAJKOVIC A UYTTENDAELE M DELEY W | 2006 | J of Microbiological Methods2006，65，3： | 1 |
| 5 | Evaluation of viral extraction methods on a broad range of Ready-To-Eat food with conventional and real-time RTPCR for Norovirus GII detection显示文摘 | BaertLeen Uyttendaele Mieke Debevere Johan | 2008 | Int J Food Microbiol2008，123，： | 1 |
| 6 | High-Quality Video View Interpolation Using a Layered Representation显示文摘 | Sing Bing Kang Matthew Uyttendaele Simon Winder Richard Szeliski | 2004 | ACM Transactions on Graphics (S0730-0301)2004，23，3： | 1 |
| 7 | Notch 4/int 3, a mammary proto-oncogene, is an endothelial cell specific mammalian Notch gene显示文摘 | Uyttendaele H Marazzi G Wu G | 1996 | Development1996，122，7： | 1 |
| 8 | Control of Aeromonas on minimally processed vegetables by decontamina- tion with lactic acid, chlorinated water, or thyme essential oil so- lution 显示文摘 | Uyttendaele M Neyts K Vanderswalmen H | 2004 | Int J Food Microbiol2004，90，3： | 1 |
| 9 | Reported foodbome outbreaks due to noroviruses in Belgium:The link between food and patient investigations in an international context显示文摘 | Baert L Uyttendaele M Stals A | 2009 | demiol Infect2009，137，3： | 1 |
| 10 | Reported foodborne outbreaks due to noroviruses in Belgium: the link between food and patient investigations in an international context显示文摘 | Baert L Uyttendaele M Stals A etal | 2009 | Epidemiol Infect2009，137，3： | 1 |
| 11 | Quantification of gene expression of Listeria monocytogenes by real-time reverse transcription PCR: optimization,evaluation and pitfalls显示文摘 | Werbrock H Bottelboom N Uyttendaele M | 2007 | J Microbiol Methods2007，9，2： | 1 |
| 12 | Characterization of growth and metabolite production of Lactobacillus reuteri during glucose/glycerol cofermentation in batch and continuous cultures显示文摘 | El -Ziney M G Arneborg N Uyttendaele M | 1998 | Biotechnology Letters1998，20，： | 1 |
| 13 | Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the Belgian retail market显示文摘 | Uyttendaele M P De Troy J Debevere | 1999 | J Food Prot1999，62，： | 1 |
| 14 | Evaluation of viral ex- traction methods on a broad range of ready - to - eat foods with con- ventional and real - time RT - PCR for Norovirus Gll detection 显示文摘 | Baert L Uyttendaele M Debevere J et el | 2008 | ln! J Food Mierobiol2008，123，1： | 1 |
| 15 | HyperISGylation of Old World monkey ISG15 in human cells显示文摘 | Pattyn E Verhee A Uyttendaele I | 2008 | PLoS ONE2008，3，6： | 1 |
| 16 | Survival ofBacillus cereus Vegetative Cells and Spores during In VitroSimulation of Gastric Passage显示文摘 | Ceuppens S Uyttendaele M Drieskens K | 2012 | J Journal of Food Protection2012，75，4： | 1 |
| 17 | Interactive Local Adjustment of Tonal Values显示文摘 | Lischinski D Farbman Z Uyttendaele M | 2006 | ACM Trans Graph2006，25，3： | 1 |
| 18 | Food safety performance indicators to benchmark food safety output of food safety management systems 显示文摘 | JACXSENS L UYTTENDAELE M DEVLIEGHERE | 2010 | International Journal of Food Microbiology2010，141，1： | 1 |
| 19 | Establishment of procedures provoking sub-lethal injury of Listeria monocytogenes, Campylobacter jejuni and Escherichia coli 0157 to serve method performance testing 显示文摘 | Jasson V Uyttendaele M Rajkovic A | 2007 | International Journal of Food Microbiology2007，118，1： | 1 |
| 20 | Antibacterial activity of Lactobacillus plantarum UG1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin UG1 显示文摘 | ENAN G E1 - ESSAWY A A UYTTENDAELE M | 1996 | International journal of food micro- biology1996，30，3： | 1 |
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