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| 1 | Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens显示文摘Background: This study evaluated the effects of Eimeria spp. challenge and dietary supplementation with free methionine or methionine dipeptide on animal performance;expression of genes associated with the immune system, antioxidant system, and amino acid transport in the jejunum;and redox status of the jejunum of broiler chickens.Methods: A randomized, 2 × 3 factorial design was used, in which Eimeria spp. challenge was the first factor(Eimeria-challenged, EC, or unchallenged, UC, broilers) and methionine supplementation was the second factor(non-supplemented, NS;free DL-methionine, DL-Met;and methionine dipeptide, DL-methionyl-DL-methionine, DLMMet). At 14 days of age, chickens were inoculated orally with sporulated oocysts of Eimeria acervulina, Eimeria praecox, Eimeria maxima, and Eimeria mitis. Birds were killed by cervical dislocation 144 h post-inoculation(PI), and the jejunum was collected for biochemical and molecular analyses.Results: EC broilers had a 13% lower feed intake(FI), 37% lower body weight gain(BWG), and 39% higher feed conversion ratio(FCR) than UC broilers. Chickens fed the DL-Met diet had higher BWG(about 12% higher) and better FCR(about 12% lower) than chickens fed the NS diet. EC chickens had lower relative weight of the bursa of Fabricius(51.8%) and higher relative weights of the spleen and whole intestine(53.6% and 26.3%, respectively) than UC chickens. Eimeria spp. challenge led to an increase in the levels of oxidative substances, such as nitrite and thiobarbituric acid reactive substances(TBARS), in the jejunum of chickens 144 h PI. Among UC chickens, those fed the DL-Met diet had higher total antioxidant capacity(TAC) and lower catalase(CAT) and superoxide dismutase(SOD) activities. EC chickens that received the NS diet had higher carbonylated protein content(CP). This result was associated with their lower TAC and catalase activity. The lower TAC in EC chickens might have been due to reduced expression of catalase(CAT) and superoxide dismutase 1(SOD1) genes. Chickens fed the DL-Met and DLMMet diets had lower nitrite content. Eimeria spp. challenge suppressed neutral amino acid transporter 1(B0 AT1),peptide transporter 1(PEPT1), toll-like receptor 5(TLR5), interleukin 2(IL2), and occludin(OCLN) gene expression and enhanced cationic amino acid transporter 1(CAT-1) and interferon gamma(IFNG) gene expression. The highest PEPT1 expression level was observed in broilers fed the DL-MMet diet, and the lowest TLR5 expression level was found in broilers fed the NS diet.Conclusion: Our results show for the first time that supplementation with methionine as free amino acid or dipeptide helps protect the intestinal cells of broilers under Eimeria spp. challenge from the oxidative damage induced by free radicals, mainly through modulation of the antioxidant system. | Angélica de Souza Khatlab Ana Paula Del Vesco Adhemar Rodrigues de Oliveira Neto Roberta Pereira Miranda Fernandes Eliane Gasparino | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 6 |
| 2 | Abnormal processing of the nociceptive input in Parkinson's disease: a study with CO2 laser evoked potentials 显示文摘 | Tinazzi M Del Vesco C Defazio G | 2008 | Pain2008，136，12： | 1 |
| 3 | High performance composite coatings on plastics: UV-curable cy- cloaliphatic expoy resins reinforced by harphene or graphene deriva- tives显示文摘 | Massimiliano Barletta Silvia Vesco Michela Puopolo | 2015 | Surface & Coatings Technology2015，，272： | 1 |
| 4 | Liquid-based cytilogy and human papillomavirus testing to screen for cervical canc- er:a systematic review for the US Preventive Services Task Force显示文摘 | Whitlock EP Vesco CC Eder M | 2011 | Ann Intern Med2011，155，： | 1 |
| 5 | Replacement of broadspectrum cephalosporins by piperacillin-tazobactam:impact on sustained high rates of bacterial resistance显示文摘 | Bantar C Vesco E Heft C | 2004 | Antimicrob Agents Chemother2004，48，2： | 1 |
| 6 | Excess gestational weight gain: modifying fetal macrosomia risk associated with maternal glucose 显示文摘 | Hillier T A Pedula K L Vesco K K | 2008 | Obstet Gynecol2008，112，5： | 1 |
| 7 | Age-related changes in mitochondrial UCP,ANT and COX HI gene expression in the breast muscleof quails ( Cotumix coturnix japonica) 显示文摘 | GASPARINO E DEL VESCO A P GUIMARAES SE | 2012 | GeneticsMolecular Research2012，11，3： | 1 |
| 8 | Diabetes mellitus after renal transplantation,characteristics,outcome,and risk factors显示文摘 | Vesco L Busson M Bedrossian J | 1996 | Transplantation1996，61，10： | 1 |
| 9 | Excess gestational weight:gain modifying fetal macromia risk associated with maternal glucose显示文摘 | Hillier TA Pedula KL Vesco KK | 2008 | Obstet Gynecol2008，112，5： | 1 |
| 10 | Dietary methionine effects on IGF-I and GHR m RNA expression in broilers显示文摘 | Del Vesco A P Gasparino E Oliveira Neto A R | 2013 | Genetics and Molecular Research2013，12，4： | 1 |
| 11 | Hyperosmotic NaCl and severe hemorrhagic shock显示文摘 | Vesco IT Pontieri V Rochae SM | 1980 | Am J Phsiol1980，239，5： | 1 |
| 12 | Pain and motor complica- tions in Parkinson's disease 显示文摘 | Tinazzi M Del Vesco C Fincati E | 2006 | J Neurol Neurosurg Psychiatry2006，77，： | 1 |
| 13 | Protocol for micropropagation of feijoa 显示文摘 | Oltramari A C Vesco L L Pedrotti E L | 2000 | Ciencia Rural2000，30，： | 1 |
| 14 | Nonhormonal therapies for menopausal hot flashes: systematic review and meta- analysis显示文摘 | NELSON H D VESCO K K HANEY E | 2006 | JAMA2006，295，17： | 1 |
| 15 | A hospitalwide intervention program to optimize the quality of antibiotic use: impact on pre- scribing practice, antibiotic consumption, cost savings, and bacterial resistance显示文摘 | Bantar C Sartori B Vesco E et aI | 2003 | Chn Infect Dis2003，37，： | 1 |
| 16 | Replacement of broad- spectrum cephalosporins by piperacillin-tazobactam: impact on sustained high rates of bacterial resistance 显示文摘 | Bantar C Vesco E Heft C | 2004 | Antimicrob Agents Chemother2004，48，2： | 1 |
| 17 | Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg)Burret):Induction,conversion and synthetic seeds显示文摘 | Gabriela Claudia Cangahuala-Inocente LI rio Luiz Dal Vesco Douglas Steinmacher | 2007 | Scientia Horticulturae2007，，111： | 1 |
| 18 | Pain and motor com- plications in Parkinson' s disease显示文摘 | Tinazzi M Vesco I)C Fincati E | 2006 | Journal of Neurology Neuro- surgery & Psychiatry2006，77，7： | 1 |
| 19 | Replacement of broad-spectrum cephalosporins by piperacillin-tazobactam: impact on sustained high rates of bacterial resistance 显示文摘 | Bantar C Vesco E Heft C | 2004 | Antimicrob Agents Chemother2004，48，2： | 1 |
| 20 | Somatic embryogenesis from floral tissues of feijoa (Feijoa sellowiana Berg)显示文摘 | Suzana Stefanello Lirio Luiz Dal Vesco Jean Pierre H J Ducroquet | 2005 | Scientia Horticulturae2005，，105： | 1 |
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