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| 1 | Geochemistry and Zircon U–Pb age of the Yao'an pseudoleucite porphyry,Yunnan Province,China显示文摘The Yao'an Pb–Ag deposit, located in the Chuxiong Basin, western Yangtze Block, is an important component of the Jinshajiang–Ailaoshan alkaline porphyry–related polymetallic intrusive belt. This complex suite of rock bodies includes a vein of pseudoleucite porphyry within deposits of syenite porphyry and trachyte.The pseudoleucite is characterized by a variable greyish,greyish-white, and greyish-green porphyritic texture. Phenocrysts are mainly pseudoleucite with small amounts of alkali feldspar and biotite. In an intense event, leucite phenocrysts altered to orthoclase, kaolinite, and quartz.Both the pseudoleucite porphyry and the syenite porphyry samples were typical alkali-rich, K-rich, al-rich rocks with high LaN/YbNratios; enriched in light rare earth elements and large-ion lithophile elements, and depleted in high field strength elements; and with strongly negative Ta, Nb, and Ti(TNT) anomalies and slightly negative Eu anomalies—all characteristics of subduction-zone mantle-derived rock.We obtained a LA-ICP-MS zircon U–Pb age of 34.1 ± 0.3 Ma(MSWD = 2.4), which is younger than the established age of the Indian and Eurasian Plate collision.The magma derived from a Type-II enriched mantle formed in a post-collisional plate tectonic setting. The geochemical characteristics of the Yao'an pseudoleucite porphyry are powerful evidence that the porphyry'sdevelopment was closely linked to the Jinshajiang–Ailaoshan fault and to the Indian-Eurasian collision. | Chundi Sun Peng Wu Die Wang Shenjin Guan Xiaojun Jiang Longyan Jiang Longyan Wang | 2017 | Acta Geochimica2017，36，2： | 8 |
| 2 | Further analyses of variation of ribosome DNA copy number and polymorphism in ciliates provide insights relevant to studies of both molecular ecology and phylogeny显示文摘Sequence-based approaches, such as analyses of ribosome DNA(rDNA) clone libraries and high-throughput amplicon sequencing, have been used extensively to infer evolutionary relationships and elucidate the biodiversity in microbial communities.However, recent studies demonstrate both r DNA copy number variation and intra-individual(intra-genomic) sequence variation in many organisms, which challenges the application of the rDNA-based surveys. In ciliates, an ecologically important clade of microbial eukaryotes, rDNA copy number and sequence variation are rarely studied. In the present study, we estimate the intraindividual small subunit rDNA(SSU r DNA) copy number and sequence variation in a wide range of taxa covering nine classes and 18 orders of the phylum Ciliophora. Our studies reveal that:(i) intra-individual sequence variation of SSU rDNA is ubiquitous in all groups of ciliates detected and the polymorphic level varies among taxa;(ii) there is a most common version of SSU rDNA sequence in each cell that is highly predominant and may represent the germline micronuclear template;(iii)compared with the most common version, other variant sequences differ in only 1–3 nucleotides, likely generated during macronuclear(somatic) amplification;(iv) the intra-cell sequence variation is unlikely to impact phylogenetic analyses;(v) the rDNA copy number in ciliates is highly variable, ranging from 103 to 106, with the highest record in Stentor roeselii. Overall,these analyses indicate the need for careful consideration of SSU r DNAvariation in analyses of the role of ciliates in ecosystems. | Yurui Wang Chundi Wang Yaohan Jiang Laura A.Katz Feng Gao Ying Yan | 2019 | Science China(Life Sciences)2019，62，2： | 7 |
| 3 | A paradox: rapid evolution rates of germline-limited sequences are associated with conserved patterns of rearrangements in cryptic species of Chilodonella uncinata(Protista, Ciliophora)显示文摘Some of the most extreme genome wide rearrangements are found in ciliates, which are unique in possessing both germline micronucleus and somatic macronucleus in every cell/organism. A series of DNA rearrangement events, including DNA elimination, chromosomal fragmentation, gene unscrambling and alternative processing, happen during macronuclear development. To assess the molecular evolution of macronuclear and germline-limited sequences in different cryptic species of Chilodonella uncinata, we characterized the actin, α-tubulin and β-tubulin genes in the micronucleus and macronucleus genomes of USA-SC2 strain and compared them with other strains(i.e. cryptic species). Three main results are:(i) rearrangement patterns between germline and soma are conserved for each gene among the cryptic species of C. uncinata;(ii) in contrast, the germlinelimited regions are highly divergent in sequence and length among the cryptic species;(iii) pointer shifting is frequent among the cryptic species. We speculate that pointer sequences may serve as the buffer between the conserved macronuclear destined sequences and rapidly-evolving internal eliminated sequences. The data combined with previous studies demonstrate the plasticity of gene rearrangement among different groups of ciliates and add to the growing data for the role of genome rearrangements in species differentiation. | Tengteng Zhang Chundi Wang Laura A.Katz Feng Gao | 2018 | Science China(Life Sciences)2018，61，9： | 5 |
| 4 | miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohn’s disease显示文摘 | Yu Chen Xiaochun Meng Chengxiao Wang Ying Liu Liwei Tang Mingxia Zheng Chundi Xu Jian Song | 2013 | Biochemical and Biophysical Research Communications2013，，： | 2 |
| 5 | miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohn’s disease显示文摘 | Yu Chen Chengxiao Wang Ying Liu Liwei Tang Mingxia Zheng Chundi Xu Jian Song Xiaochun Meng | 2013 | Biochemical and Biophysical Research Communicatio2013，，1： | 2 |
| 6 | Non-filamentous sludge bulking caused by a deficiency of nitrogen in industrial wastewater treatment显示文摘 | Peng Yongzhen Gao Chundi Wang Shuying | 2003 | Water Science and Technology2003，47，11： | 1 |
| 7 | Combination of weighted ? 2,1 minimization with unitary transformation for DOA estimation显示文摘 | Chundi Zheng Gang Li Xiqin Wang | 2013 | Signal Processing2013，，： | 1 |
| 8 | Subspace weighted ?2,1 minimization for sparse signal recovery显示文摘 | Chundi Zheng Gang Li Yimin Liu Xiqin Wang | 2012 | EURASIP Journal on Advances in Signal Processing2012，，1： | 1 |
| 9 | Enzyme immunoassay for the determination of carbaryl residues in agricultural products显示文摘 | Shuo Wang Chundi Yu Junping Wang | 2005 | Food Additives & Contaminants: Part A2005，，8： | 1 |
| 10 | miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohn’s disease显示文摘 | Yu Chen Xiaochun Meng Chengxiao Wang Ying Liu Liwei Tang Mingxia Zheng Chundi Xu Jian Song | 2013 | Biochemical and Biophysical Research Communications2013，，： | 1 |
| 11 | Timing and characteristics of nuclear events during conjugation and genomic exclusion in Paramecium multimicronucleatum显示文摘Ciliated protists are ideal material for studying the origin and evolution of sex,because of their nuclear dimorphism(containing both germline micronucleus and somatic macronucleus in the same cytoplasm),special sexual processes(conjugation and autogamy),and high diversity of mating-type systems.However,the study of sexual process is limited to only a few species,due to the difficulties in inducing or observing conjugation.In the present study,we investigate the conjugation process in Paramecium multimicronucleatum:(1)of the three prezygotic divisions,all micronuclei undergo the first two divisions(meiosis I,II),while a variable number of nuclei undergo the third division(mitosis);(2)the synkaryon divides three times after fertilization,giving rise to eight products that differentiate into four macronuclear anlagen and four micronuclei;(3)cells restore the vegetative stage after two successive cell fissions during which the macronuclear anlagen are distributed into daughter cells without division,while micronuclei divide mitotically;(4)the parental macronucleus begins to fragment following the first meiotic division and finally degenerates completely;(5)the entire process takes about 110 h,of which about 85 h are required for macronuclear development.In addition,we describe for the first time the process of genomic exclusion occurring between amicronucleate and micronucleate cells of P.multimicronucleatum,during which the micronucleate cell contributes a pronucleus to the amicronucleate cell,resulting in both exconjugants being homozygotes.These results provide new insights into the diversity of sexual processes and lay an important cytological basis for future in-depth studies of mating systems in ciliates. | Xue Zhang Xiaoteng Lu Yong Chi Yaohan Jiang Chundi Wang Saleh AAl‑Farraj Adriana Vallesi Feng Gao | 2022 | Marine Life Science & Technology2022，4，3： | 1 |
| 12 | Pre-liminary study显示文摘 | Xu Chundi Wu Yunlin Mei Hong Wang chien-hsiu*Xu Jia-yu | 1995 | Chinese Journal of Pediatrics emergency gastroscopyand alimentary tract bleeding in1995，，01： | 1 |
| 13 | miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohn’s disease显示文摘 | Yu Chen Xiaochun Meng Chengxiao Wang Ying Liu Liwei Tang Mingxia Zheng Chundi Xu Jian Song | 2013 | Biochemical and Biophysical Research Communications2013，，： | 1 |
| 14 | What are the appropriate mapping units for ecosystem service assessments?A systematic review显示文摘Context:Without clear understanding of the units used for ecosystem service(ES)mapping,ES assessment accuracy and the practical application of ES knowledge will be hampered.Method:We systematically reviewed 106 studies over the past 11 years to explore the type,characteristic pattern and deficiencies of mapping units.Result:We proposed that ES mapping units can be categorized into minimal unit for assessing ESs using corresponding indicators and methods,and aggregated unit for analysis and application based on research objectives,and classified the mapping units into five common types.Of the 12 characterizing variables of ES mapping studies,some have been shown to introduce a difference in the selection of mapping units and to exhibit characteristic patterns.We also found that the accuracy of ES assessments based on minimal units was lacking,and aggregated units were insufficient to establish a link between ES knowledge and practice.Conclusion:Herein,we propose possible solutions such as the use of fine spatial resolution grids and the introduction of additional data beyond land cover as supplements to improve the assessment accuracy.To enhance the availability of the results for practice,aggregated units connected with urban planning units should be established at a spatial level suitable for urban management. | Jiake Shen Chundi Chen Yuncai Wang | 2021 | Ecosystem Health and Sustainability2021，7，1： | 1 |
| 15 | Invasion by exogenous RNA:cellular defense strategies and implications for RNA inference显示文摘Exogenous RNA poses a continuous threat to genome stability and integrity across various organisms.Accumulating evidence reveals complex mechanisms underlying the cellular response to exogenous RNA,including endo-lysosomal degradation,RNA-dependent repression and innate immune clearance.Across a variety of mechanisms,the natural anti-sense RNAdependent defensive strategy has been utilized both as a powerful gene manipulation tool and gene therapy strategy named RNA-interference(RNAi).To optimize the efficiency of RNAi silencing,a comprehensive understanding of the whole life cycle of exogenous RNA,from cellular entry to its decay,is vital.In this paper,we review recent progress in comprehending the recognition and elimination of foreign RNA by cells,focusing on cellular entrance,intracellular transportation,and immune-inflammatory responses.By leveraging these insights,we highlight the potential implications of these insights for advancing RNA interference efficiency,underscore the need for future studies to elucidate the pathways and fates of various exogenous RNA forms,and provide foundational information for more efficient RNA delivery methods in both genetic manipulation and therapy in different organisms. | Danxu Tang Yan Liu Chundi Wang Lifang Li Saleh AAl‑Farraj Xiao Chen Ying Yan | 2023 | Marine Life Science & Technology2023，5，4： | 1 |
| 16 | Effects of glutamine pretreatment on learning and memory in heat-exposed rats显示文摘BACKGROUND: Glutamine (Gln) pretreatment can protect neural cells from injuries due to heat, ischemia, hypoxia, endotoxemia, and inflammatory factors. OBJECTIVE: To observe the effects of Gln pretreatment on learning and memory, survival time, and rectal temperature in heat-exposed rats. DESIGN, TIME AND SETTING: The present randomized grouping, neurobehavioral experiment was performed at the Laboratory of Department of Pharmacology, Basic School of Medicine, Wuhan University between March and September 2007. MATERIALS: Twenty-four healthy, Wistar rats were included in this study. SPX-160B biochemistry incubator (Shanghai Experimental Equipment Co., Ltd., China), probe electronic thermometer (11000 type, Maikepai Science and Technology Co., Ltd., China), Y-type maze box used in conjunction with MG-2 maze stimulator (Zhangjiagang Biomedical Instrument Factory, China), L-Gln (Batch No. 061218, 5 g/bottle, prepared into 10% aqueous solution, Amresco Company, USA) were used. METHODS: Twenty-four rats were randomly and evenly divided into 3 groups: heat-exposed, Gln low-lose, and Gln high-dose. Following learning and memory testing with the Y-maze, rats in the heat-exposed group were subjected to heat injury (40.5-41.5 ℃) in a biochemistry incubator. Rectal temperature was measured every 5 minutes. Thirty-five minutes after heat exposure, rats were removed and placed in the Y-type maze to test learning and memory again. Subsequently, the rats were returned to the same environment of thermal stimulation until they died. Rat survival time was recorded. Subsequent to learning and memory testing, rats in the Gln low-dose and high-dose groups received an i.p. injection of Gln (0.4 g/kg and 0.8 g/kg, respectively), and were exposed to heat injury. The remaining experimental procedures remained the same as for the heat-exposed group. MAIN OUTCOME MEASURES: Rat learning and memory, rectal temperature, and survival time in heat exposure environment. RESULTS: (1) In the Y-maze experiment, the frequency for the heat-exposed group rats to move 15 successive times through the maze without any mistakes was 13 times greater prior to heat exposure. Thirty-five minutes after heat exposure, rat learning and memory were significantly decreased, i.e., frequency was 9/15 correct trials (P < 0.01). Following Gln pretreatment, rat learning and memory noticeably improved. The frequency of Gln low-dose and high-dose rats maintained 13/15 correct trials, which was not different compared with before heat exposure (P > 0.05). (2) The heat-exposed group required 14 minutes to reach the rectal temperature of 39.5 ℃. After 2 hours of Gln pretreatment, the Gln low-dose and high-dose groups needed 18 and 20 minutes, respectively (P < 0.05). (3) Following 2 hours of Gln pretreatment, the survival time of the Gln low-dose and high-dose groups was 66 and 69 minutes, which was prolonged by 18.2% (P < 0.05) and 21.7% (P < 0.01), respectively, compared with the heat-exposed group (54 minutes). CONCLUSION: A 2-hour Gln pretreatment can noticeably improve learning and memory, delay ascending speed of body temperature, and prolong survival time in heat-exposed rats in a dose-dependent manner. | Shenghao Zhao Lei Wang Qin Wang Siyi Wang Chundi Deng Xianfei Xie Youe Yan Hui Wang | 2008 | Neural Regeneration Research2008，3，10： | 1 |
| 17 | New contributions to the phylogeny of the ciliate class Heterotrichea(Protista,Ciliophora):analyses at family-genus level and new evolutionary hypotheses显示文摘Heterotrichous ciliates play an important role in aquatic ecosystem energy flow processes and many are model organisms for research in cytology,regenerative biology,and toxicology.In the present study,we combine both morphological and molecular data to infer phylogenetic relationships at family-genus level and propose new evolutionary hypotheses for the class Heterotrichea.The main results include:(1)96 new ribosomal DNA sequences from 36 populations,representing eight families and 13 genera,including three poorly annotated genera,Folliculinopsis,Ampullofolliculina and Linostomella;(2)the earliest-branching families are Spirostomidae in single-gene trees and Peritromidae in the concatenated tree,but the family Peritromidae probably represents the basal lineage based on its possession of many'primitive'morphological characters;(3)some findings in molecular trees are not supported by morphological evidence,such as the family Blepharismidae is one of the most recent branches and the relationship between Fabreidae and Folliculinidae is very close;(4)the systematic positions of Condylostomatidae,Climacostomidae,and Gruberiidae remain uncertain based either on morphological or molecular data;and(5)the monophyly of each genus included in the present study is supported by the molecular phylogenetic trees,except for Blepharisma in the SSU r DNA tree and Folliculina in the ITS1-5.8 S-ITS2 tree. | Yong Chi Xiangrui Chen Yuqing Li Chundi Wang Tengteng Zhang Alex Ayoub Alan Warren Weibo Song Yuanyuan Wang | 2021 | Science China(Life Sciences)2021，64，4： | 0 |
| 18 | Asymptotic Behavior of the Solution to a 3-D Simplified Energy-Transport Model for Semiconductors显示文摘 | LIU Chundi LI Yong WANG Shu | 2016 | Journal of Partial Differential Equations2016，29，1： | 0 |
| 19 | New considerations on the phylogeny of Sessilida(Protista:Ciliophora:Peritrichia)based on multiple-gene information,with emphasis on colonial taxa显示文摘The subclass Peritrichia,containing two orders Sessilida and Mobilida,is a major group of ciliates with worldwide distribution and high species diversity.Several studies have investigated the phylogeny of peritrichs;however,the evolutionary relationships and classification of some families and genera within the Sessilida remain unclear.In the present study,we isolated and identified 22 peritrich populations representing four families and six genera and obtained 64 rDNA sequences to perform phylogenetic analyses and assess their systematic relationships.Ancestral character reconstruction was also carried out to infer evolutionary routes within the Sessilida.The results indicate:(1)family Vaginicolidae is monophyletic and acquisition of the typical peritrich lorica represents a single evolutionary divergence;(2)core epistylidids evolved from a Zoothamnium-like ancestor and experienced spasmoneme loss during evolution;(3)Campanella clusters with species in the basal clade and shows stable morphological differences with other epistylidids,supporting its assignment to a separate family;(4)the structure of the peristomial lip may be a genus-level character rather than a diagnostic character for discriminating Epistylididae and Operculariidae,thus a redefinition of Operculariidae should be carried out when more species have been investigated;(5)some characters,such as lifestyle(solitary or colonial),spasmoneme and living habit(sessile or free-swimming),evolved repeatedly among sessilids indicating that species with non-contractile stalks or that are free-swimming have multiple evolutionary routes and might derive from any sessilid lineage without a lorica.The close phylogenetic relationships of some morphologically distinct sessilids imply that the diagnoses of some genera and families should be improved. | Zhe Wang Xiaoting Feng Lifang Li Khaled A.S.Al-Rasheid Alan Warren Chundi Wang Zemao Gu | 2022 | Marine Life Science & Technology2022，4，4： | 0 |
| 20 | Restoring Vision Naturally and Noninvasively显示文摘More than 200 million people across the world suffer from moderate to severe visual impairments,mostly due to retinal degeneration or damage to the eyes[1].Methods of reprogramming the cells in the eyes or electrical stimulation can be used to restore vision in acquired blindness[2]. | Chundi Wang Hu Deng Shenbing Kuang | 2021 | Neuroscience Bulletin2021，37，11： | 0 |
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