|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector显示文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction. | Xiao B Jing B Zhang YL Zhou DY Zhang WD | 2000 | World Journal of Gastroenterology2000，6，1： | 11 |
| 2 | Regional differences in HIV prevalence among drug users in China: potential for future spread of HIV?显示文摘 | Kretzschma M Zhang WD Mikolajczyk RT | 2008 | BMC Infect Dis2008，8，： | 1 |
| 3 | The propeptide of macrophage inhibitory cytokine (MIC-1),a TGF-beta superfamily member,acts as a quality control determinant for correctly folded MIC-1显示文摘 | Bauskin AR Zhang HP Fairlie WD | | 0，，10： | 1 |
| 4 | TNF-related apoptosis-inducing ligand-indced apoptosis of melanoma is associated with changes in mitochondrial membrane potential and perinuclear clustering of nitochondria 显示文摘 | Thomas WD Zhang XD Franco AV | 2000 | Immunology2000，165，10： | 1 |
| 5 | Computed tomography and magnetic resonance imaging findins of solitary fibrous tumours in the pelvis:Correlation with histopathological findings 显示文摘 | Zhang WD Chen JY Cao Y | 2011 | Eur J Radiol2011，78，1： | 1 |
| 6 | Serum antitoxin antibodies mediate systemic and mucosal protection from Clostridium difficile disease in hamsters显示文摘 | Paul JG Zhang ZX Lei WD | 1999 | Infect Immun1999，67，： | 1 |
| 7 | 显示文摘 | Huang WD Zhang J | 2003 | Proc Natl Acad Sci2003，100，7： | 1 |
| 8 | Functional sensory function recovery of random - panem abdominal skin flap in the re- pair of fingertip skin defects 显示文摘 | Yu YD Zhang YZ Bi WD | 2013 | Exp Ther Med2013，5，3： | 1 |
| 9 | Process parameters selection in NC camshaft grinding based on uniform design and GA-NN显示文摘 | DENG Zhaohui ZHANG Xiaohong LIU Wd | 2009 | Advanced Materials Research2009，6970，： | 1 |
| 10 | The propeptide of maerophage inhibitory cytokine (MIC-1), a TGF beta super- family member, acts as a quality control determinant for cor- rectly folded MIC-1显示文摘 | Bauskin AR Zhang HP Fairlie WD | 2000 | EMBO J2000，19，10： | 1 |
| 11 | Prognostic significance of osteopontin in hepatocellular carcinoma, a meta-analysis 显示文摘 | ZHANG CH XU GL JIA WD | 2012 | Int J Cancer2012，130，： | 1 |
| 12 | Traumatic injury induced homer-1a gene expression in cultured conical neurons of rat显示文摘 | Huang WD Fei Z Zhang X | 2005 | Neurosci Lett2005，389，1： | 1 |
| 13 | Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture pattems显示文摘 | NIE X TIAN WD ZHANG YJ | 2006 | Cell Biol Int2006，30，： | 1 |
| 14 | Iridoids from Pedicu- laris kansuensis forma albiflora 显示文摘 | Yuan CS Zhang Q Xie WD | 2003 | Pharmazie2003，58，6： | 1 |
| 15 | Effects of tumor necrosis factor-αinhibitors on new bone formation in ankylosing spondylitis显示文摘 | Zhang JR Liu XJ Xu WD | 2015 | Joint Bone Spine2015，，： | 1 |
| 16 | The use of gated and 4D CT imaging in planning for stereotactic body radiation therapy显示文摘 | D'Souza WD Nazareth DP Zhang B | 2007 | Med Dosim2007，32，2： | 1 |
| 17 | A key review on emergy analysis and assessment of biomass resources for a sustainable future显示文摘 | Zhang GJ Long WD | | 0，，： | 1 |
| 18 | Image classification of liver cancer surrounding right hepatic pedicle and its guide to precise liver resection显示文摘 | Chen XP Zhang WD Wang D | 2015 | Int J Clin Exp Med2015，8，11： | 1 |
| 19 | Up-regulation of platelet-derived growth factor-A is responsible for the failure of re-initiated interferon alpha treatment in hepatocellular carcinoma显示文摘 | Zhang JB Sun HC Jia WD | 2012 | BMC Cancer2012，12，： | 1 |
| 20 | Endevascular therapy and arterial bypass for subclavian artery oeclosion显示文摘 | Wang KQ Yuan C Zhang WD | 2006 | Chin J Surg2006，44，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员