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| 1 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 2 | 热应激和低营养摄入对猪肠道完整性和功能的影响显示文摘热应激会损害肠道完整性,并诱发肠渗漏。因此,本试验研究了直接或间接热应激(通过减少采食量)对生长猪肠道通透性的影响。选用体重(43±4)kg的杂交后备母猪(n=48),分别安置在以下控制环境中:热中性(TN)环境(20℃,湿度35%~50%,自由采食)、热应激(HS)环境(35℃,湿度20%~35%,自由采食)、热中性环境配对饲喂(PFTN,以消除不同采食量的交互影响)。第1、3、7天使猪只安乐死,采集的空肠样品被安装到尤式灌流室,测定跨膜电阻抗值(TER)和异硫氰基荧光标记的脂多糖(LPS)渗透性(APP,表示为表观渗透系数)。此外,评估肠道完整性以及应激基因和蛋白标志物。结果表明,不考虑热应激天数,HS组与TN组猪相比,血浆内毒素水平增加45%(P〈0.05),空肠TER降低30%(P〈0.05),LPS渗透率增加2倍(P〈0.01)。此外,与PFTN组相比,HS组第7天的猪LPS渗透性有增加的趋势(P=0.06)。整个试验阶段HS组溶菌酶和碱性磷酸酶活性下降,分别下降46%和59%(P〈0.05);然而,3 d和7 d空肠免疫细胞标记物——髓过氧化物酶活性增加(P〈0.05)。由此可见,热应激和减少采食量都会降低肠道完整性,并增加内毒素渗透性。推测这可能会导致炎症加重,在炎热的夏季可能是导致猪生长性能降低的原因。 | Pearce S C Mani V Weber T E Rhoads R P Patience J F Baumgard L H Gabler N K | 2016 | 中国饲料2016，，3： | 3 |
| 3 | 24h动态中心动脉收缩压和左心室质量的关系:一项前瞻性多中心研究显示文摘研究者在7个欧洲中心中调查左心室质量与肱动脉诊室血压以及肱动脉压和中心动脉动态收缩压之间的关系。使用校准的示波装置测量,经平均压/舒张压校准和转换函数计算中心动脉收缩压。由不知道血压值的单个操作者通过M型超声心动图测定左心室质量。研究者调查了未使用抗高血压药物的受试者289人(女性137人,平均年龄50.8岁)。 | 黄洁 叶鹏 Weber T Wassertheurer S Schmidt-Trucks?ss A Rodilla E Ablasser C Jankowski P Lorenza Muiesan M Giannattasio C Mang C Wilkinson I Kellermair J Hametner B Pascual JM Zweiker R Czarnecka D Paini A Salvetti M Maloberti A McEniery C | 2017 | 中华高血压杂志2017，25，11： | 2 |
| 4 | Seed yield losses from lodging combine harvesting in soybeans 显示文摘 | Weber C R Fehr W R | 1966 | Agronomy Journal1966，58，： | 1 |
| 5 | Effective Bandwidths for Stationary Source显示文摘 | Weber R | 1995 | Probability in Engineering and Informational Sceinces1995，9，2： | 1 |
| 6 | Vendor Selection Criteria and Methods显示文摘 | Weber C A Current J R Benton W C | 1991 | European Journal of Operational Research1991，50，： | 1 |
| 7 | Hyperglycaemiablocks sevofluraneinduced postconditioning in the rat heart invivo:cardioprotection can be restored by blocking the mito-chondrial permeability transition pore显示文摘 | Hunu R Heinen A Weber N C | 2008 | Br J Anaesth2008，100，4： | 1 |
| 8 | Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1 显示文摘 | Bouyer P G Tang X Weber C R | 2013 | Am J Physiol Gastrointest Liver Physio12013，304，2： | 1 |
| 9 | Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.)显示文摘 | A. B?rner E. Schumann A. Fürste H. C?ster B. Leithold M. R?der W. Weber | 2002 | Theoretical and Applied Genetics (-)2002，，6： | 1 |
| 10 | Adaptation of HIV-1to human leukocyte antigen class I显示文摘 | Kawashima Y Pfafferott K Frater J Matthews P Payne R Addo M Gatanaga H Fujiwara M Hachiya A Koizumi H Kuse N Oka S Duda A Prendergast A Crawford H Leslie A Brumme Z Brumme C Allen T Brander C Kaslow R Tang J Hunter E Allen S Mulenga J Branch S Roach T John M Mallal S Ogwu A Shapiro R Prado J G Fidler S Weber J Pybus O G Klenerman P Ndung'u T Phillips R Heckerman D Harrigan P R Walker B D Takiguchi M Goulder P | 2009 | Nature2009，458，7238： | 1 |
| 11 | Testing and demonstration results of the 350 m long HTS cable system installed in albany, NY 显示文摘 | WEBER C S LEE R RINGO S | 2007 | IEEE Trans Appl Supercond2007，17，2： | 1 |
| 12 | Accumulation of N,P and K by soybean (Glycine max (L) Merrill) plants显示文摘 | Hanway J J Weber C R | 1971 | Agronomy Journal1971，63，： | 1 |
| 13 | Lenalidomide plus dexamethasone for relapsed multiple myeloma in North America 显示文摘 | WEBER DM CHEN C NIESVIZKY R | 2007 | N Engl J Med2007，357，21： | 1 |
| 14 | A limited set of human microRNA is deregulated in follicular thyroid carcinoma 显示文摘 | WEBER F TERESI R E BROELSCH C E | 2006 | J Clin Endocrinol Metab2006，91，9： | 1 |
| 15 | Vendor Selection Criteria and Methods显示文摘 | CURRENT J R BENTON W C | 1991 | European Journal of Operational Research1991，50，： | 1 |
| 16 | A multiobjective approach to vendor selection显示文摘 | Weber C A Current J R | 1993 | European Journal of Operational Research1993，68，2： | 1 |
| 17 | Interception of solar radiation and dry matter production by various soybean planting patterns显示文摘 | Shibles R M C R Weber | | 0，，： | 1 |
| 18 | Laparoscopic adrenalectomy: new gold standard 显示文摘 | Smith CD Weber C J Amerson R | 1999 | World J Surg1999，23，4： | 1 |
| 19 | Vendor Selection Criteria and Methods 显示文摘 | Weber C A Current J R Benton W C | 1991 | European Journal of Operational Research1991，，50： | 1 |
| 20 | The kinetics of alpha-decay-induced amorphization in zircon and apatite containing weapons-grade plutonium or other actinides显示文摘 | WEBER W J EWING R C MELDRUM A | 1997 | J Nucl Mate1997，250，： | 1 |
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