|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 2 | TT viral infection through blood transfusion:retrospective investigation on patients in a prospective study of post-transfusion hepatitis显示文摘AIM To investigate the role of bloodtransfusion in TT viral infection(TTV).METHODS We retrospectively studied serumsamples from 192 transfusion recipients whounderwent cardiovascular surgery and bloodtransfusion between July 1991 and June 1992.Allpatients had a follow-up every other week for atleast 6 months after transfusion.Eightyrecipients received blood before screeningdonors for hepatitis C antibody(anti-HCV),and112 recipients received screened blood.Recipients with alanine aminotransferase level>2.5 times the upper normal limit were testedfor serological markers for viral hepatitis A,B,C,G,Epstein-Barr virus and cytomegalovirus.TTV infection was defined by the positivity forserum TTV DNA using the polymerase chainreaction method.RESULTS Eleven and three patients,whoreceived anti-HCV unscreened and screened'blood,respectively,had serum ALT levels>90 IU/L.Five patients(HCV and TTV:1;HCV,HGV,and TTV:1;TTV:2;and CMV and TTV:1)were positive for TTV DNA,and four of them hadsero-conversion of TTV DNA.CONCLUSION TTV can be transmitted viablood transfusion.Two recipients infected byTTV alone may be associated with the hepatitis.However,whether TTV was the causal agentremains unsettled,and further studies arenecessary to define the role of TTV infection inchronic hepatitis. | Yang SS Wu CH Chen TH Huang YY Huang CS | 2000 | World Journal of Gastroenterology2000，6，1： | 8 |
| 3 | Pulmonary infection control window in treatment of severe respiratory failure of chronic obstructive pulmonary diseases: a prospective, randomized controlled, multi-centred study显示文摘Background Early withdraw from invasive mechanical ventilation (MV) followed by noninvasive MV is a new strategy for changing modes of treatment. This study was conducted to estimate the feasibility and the efficacy of early extubation and sequential noninvasive MV commenced at beginning of pulmonary infection control window in patients with exacerbated hypercapnic respiratory failure caused by chronic obstructive pulmonary diseases (COPD). Methods A prospective, randomized controlled study was conducted in eleven teaching hospitals' respiratory or medical intensive care units in China. Ninety intubated COPD patients with severe hypercapnic respiratory failure triggered by pulmonary infection (pneumonia or purulent bronchitis) were involved in the study. When the pulmonary infection had been controlled by antibiotics and comprehensive therapy, the 'pulmonary infection control window (PIC window)' has been reached. Each case was randomly assigned to study group (extubation and noninvasive MV via facial mask immediately) or control group (invasive MV was received continuously after PIC window by using conventional weaning technique).Results Study group (n=47) and control group (n=43) had similar clinical characteristics initially and at the time of PIC window. Compared with control group, study group had shorter duration of invasive MV [(6.4±4.4) days vs (11.3±6.2) days, P=0.000], lower rate of ventilator associated pneumonia (VAP) (3/47 vs 12/43, P=0.014), fewer days in ICU [(12±8) days vs (16±11) days, P=0.047] and lower hospital mortality (1/47 vs 7/43, P=0.025).Conclusions In COPD patients requiring intubation and invasive MV for hypercapnic respiratory failure, which is exacerbated by pulmonary infection, early extubation followed by noninvasive MV initiated at the start of PIC window may decrease significantly the duration of invasive MV, the risk of VAP and hospital mortality. | Wang, C Zhan, QY Cao, ZX Wei, LQ Cheng, ZZ Liu, S Zhang, JL Chen, RC Luo, Q Niu, SF Zhu, L Wu, DW Fang, BM Wu, TH Wang, CZ Liu, YN | 2005 | Chinese Medical Journal2005，，19： | 6 |
| 4 | Association between high-sensitivity cardiac troponin T and N-terminal pro-brain natriuretic peptide in a community based population显示文摘 | Xu RY Ye P Luo LM Sheng L Wu HM Xiao WK Zheng J Wang F Xiao TH | 2014 | Chinese Medical Journal2014，，4： | 2 |
| 5 | Relationship Between Soil Properties and Different Fractions of Soil Hg显示文摘Correlation and path analysis methods were used to study the relationship between soil properties and the distribution of different soil Hg fractions with nine representative soils from Chongqing, China. Results showed that clay (< 2 m) could increase water-soluble Hg (r = 0.700*). Soil organic matter (OM) could enhance the increase of elemental Hg (r = 0.674*). The higher the base saturation percentage (BSP), the more the residual Hg (T = 0.684*). Organic Hg, the sum of acid-soluble organic Hg and alkali-soluble Hg, was positively affected by silt (2-20 μm) but negatively affected by pH, with the direct path coefficients amounting to 1.0487 and 0.5121, respectively. The positive effect of OM and negative effect of BSP on organic Hg were the most significant, with the direct path coefficients being 0.7614 and -0.8527, respectively. The indirect effect of clay (< 2 μm) via BSP (path coefficient = 0.4186) was the highest, showing that the real influencing factor in the effect of clay (< 2 μm) on acid-soluble organic Hg was BSP. Since the available Hg fraction, water-soluble Hg, was positively affected by soil clay content, and the quite immobile and not bioavailable residual Hg by soil BSP, suitable reduction of clay content and increase of BSP would be of much help to reduce the Hg availability and Hg activity in Hg-contaminated soils. | WU HONGTAO YU GUIFEN QING CHANGLE and CHEN TONGBIN Southwest Agricultural University, Chongqing 400716 (China) Institute of Geographic Sciences and Natural Resources, the Chinese Academy of Sciences, Beijing 100101 (China) Institute of Soil Science, th | 2001 | Pedosphere2001，11，3： | 2 |
| 6 | Metals and organochlorine pesticides in caudal scutes of crocodiles from Belize and Costa Rica显示文摘 | Wu TH Finger AG | 2007 | Science of the Total Environment2007，373，1： | 1 |
| 7 | Survival analysis in systemic lupus cry- thematosus patients on maintenance dialysis: a nationwide population- based study in Taiwan显示文摘 | Chang YS Liu CJ Wu TH | 2013 | Rheumatology (Oxford)2013，52，1： | 1 |
| 8 | Anti - tumor effect of pEgr- IFNgamma gene - radiotherapy in B16 melanoma -bearing mice显示文摘 | Wu CM Li XY Huang TH | 2004 | World J Gastroenterol2004，10，20： | 1 |
| 9 | Excessive expression of thetumor necI'osis factor~alpha gene in the kidneys of patients with membranous glomerulonephritis显示文摘 | Wu TH Tsai CY Yang WC | 1998 | Chung Hua I HsuehTsa Chih Taipei1998，61，9： | 1 |
| 10 | Can iterative reconstruction improve imaging quality for lower radiation CT perfusion? Initial experience 显示文摘 | Lin C J Wu TH Lin C H | 2013 | AJNR2013，34，： | 1 |
| 11 | Location and size of infarct on functional outcome of noncardioembolic ischemic stroke显示文摘 | Pan SL Wu SC Wu TH | 2006 | Disabil Rehabil2006，28，： | 1 |
| 12 | Measurement of free hydroxytyrosol in microdialysates from blood and brain of anesthetized rats by liquid chromatography with fluorescence detection显示文摘 | WU YT LIN LC TSAI TH | 2009 | J Chrom-atogr A2009，1216，16： | 1 |
| 13 | The effects of carpentry on heterotopic ossification and mobility in cervical arthroplasty:determination by computed tomography with a minimum 2-year follow-up: Clinical article 显示文摘 | Tu TH Wu JC Huang WC | 2012 | J Neurosurg Spine2012，16，6： | 1 |
| 14 | The Automated Classification of Medi- cal Entities (ACME) system objectively assessed the appropriate- ness of underlying cause-of-death certification and assignment 显示文摘 | Lu TH Tsau SM Wu TC | 2005 | J Clin Epidemiol2005，58，12： | 1 |
| 15 | Bilateral simultaneous tubal sextuplets:pregnancy after in-vitro fertilization--embryo transfer following salpingectomy显示文摘 | Chang CC Wu TH Tsai HD | 1998 | Hum Reprod1998，13，： | 1 |
| 16 | Lactated Ringer's solution re- duces systemic inflammation compared with saline in patients with acute pancreatitis 显示文摘 | Wu BU Hwang JQ Gardner TH | 2011 | Clinical Gastroenterology and Hepatology2011，9，8： | 1 |
| 17 | Effects of pH adjustment and heat treatment on the stability and the formation of volatile compounds of garlic 显示文摘 | Yu Th Wu CM Chen SY | 1989 | J Agric Food Chem1989，37，： | 1 |
| 18 | Preparation, physicochemical characterization, and antioxidant effects of quercetin nanoparticles显示文摘 | Wu TH Yen FL Lin LT | 2008 | International Journal of Pharmaceutics2008，346，12： | 1 |
| 19 | Multidisciplinary group behavioral and pharmacologic intervention for cardiac risk reduction in diabetes:a pilot study显示文摘 | Martin OJ Wu WC Taveira TH | | 0，，01： | 1 |
| 20 | Preparation, physicochemical charac- terization,and antioxidant effects of quercetin nanoparticles 显示文摘 | Wu TH Yen FL Lin LT | 2008 | Int J Pharm2008，346，12： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员