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| 1 | Construction of a genetic linkage map for cotton based on SRAP显示文摘A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers. | LIN Zhongxu, ZHANG Xianlong, NIE Yichun, HE Daohua & WU Maoqing National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China | 2003 | Chinese Science Bulletin2003，48，19： | 111 |
| 2 | Analysis of in vivo patterns of caspase 3 gene expression in primary hepatocellular carcinoma and its relationship to p21^(WAF1) expression and hepatic apoptosis显示文摘AIM To detect the expression of caspase 3gene in primary human hepatocellular carcinoma(HCC)and investigate its relationship to p21WAF1gene expression and HCC apoptosis.METHODS In situ hybridization was employedto determine caspase 3 and p21WAF1expression inHCC.In situ end-labeling was used to detecthepatocytic apoptosis in HCC.RESULTS Twenty-one of 39(53.8%)cases ofHCC were found to express caspase 3transcripts,while 45.2% of HCC failed toexpress caspase 3.Non-cancerous adjacent livertissues showed more positive caspase 3(87.5%,7/8)as compared with HCC(P<0.05).The expression of caspase 3 is correlated withHCC differentiation,72.2%(13/18)ofmoderately to highly differentiated HCC showedcaspase 3 transcripts positive,while only 38.1%of poorly differentiated HCC harbored caspase 3transcripts(P<0.05).No relationship wasfound between caspase 3 expression and tumorsize or grade or metastasis,although 52.5%(5/8)of HCC with metastasis were caspase 3positive and a little higher than that with nometastasis(51.6%,P>0.05).Expression of caspase 3 alone did not affect the apoptosisindex(AI)of HCC.The AI was 7.12%o in caspase3-positive tumors(n=21),while in caspase 3-negative cases(n=18)6.59%0(P>0.05).Expression of caspase 3 clearly segregated withp21WAF1positive tumors as compared withp21WAF1-negative cases(16 of 23,69.6% versus5 of 16,31.3%)with statistical significance(P=0.017).In the cases with positive caspase 3and negative p21WAF1,the Al was found slightlyhigher,but with no statistical significance,thanthat with expression of p21WAF1and caspase 3(7.21‰ vs 6.98‰,P>0.05).CONCLUSION Loss of caspase 3 expressionmay contribute to HCC carcinogenesis,althoughthe expression of caspase 3 does not correlatewell with cell apoptosis in HCC.p21WAF1may bemerely one of the inhibitors which can reducecaspase 3 mediated cell apoptosis in HCCs. | Bao Hua Sun Jun Zhang Bao JǜWang Xi Ping Zhao You Kun Wang Zhi Qun Yu Dong Liang Yang Lian Jie Hao Department of Clinical Immunology,Tongji Hospital,Tongji Medical University,Wuhan 430030,Hubei Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 65 |
| 3 | Petrogenesis and dating of the Kangding complex,Sichuan Province显示文摘An analysis of major and trace element geochemistry and SHRIMP U-Pb zircon dating of mafic, intermediate, and felsic rocks from the Mianning metamorphic complex of Si- chuan Province was conducted. One of our conclusions is that a majority of the zircons crystal- lized between 721 and 773 Ma in a magmatic regime. The complexes contain early to late Pa- leo-proterozoic relict zircons from ancient continental crust. The oldest age, 2468 Ma, may rep- resent the basement of the Yangtze block. Overgrowth zircon rims and palingenetic zircons have been observed and dated, and are believed to have formed after crystallization, during Paleozoic and Mesozoic metamorphic-anatexis. We also conclude that depletion of Nb, Ta, HREE, in a spider-diagram normalized to primitive mantle and calc-alkaline association, implies that they formed in island-arc or under-plating settings. | CHEN Yuelong 1 , LUO Zhaohua 1 , ZHAO Junxiang, LI Zhihong, ZHANG Hongfei & SONG Biao 3 1. Faculty of Earth Sciences and Natural Resources, China University of Geosciences, Beijing 100083, China 2. Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074, China 3. Beijing SHRIMP Center, Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China | 2005 | Science China Earth Sciences2005，48，5： | 40 |
| 4 | Changes of plasma D(-) -lactate, diamine oxidase and endotoxin in patients with liver cirrhosis显示文摘BACKGROUND: Plasma D(-)-lactate and diamine oxidase (DAO) can reflect patients’ intestinal mucosal condition. We evaluated the changes of plasma D (-)-lactate, DAO and endotoxin activities and their significance in patients with liver cirrhosis. METHODS: Fifty liver cirrhosis patients were enrolled into experimental group and 30 healthy people into control group. The plasma levels of D(-)-lactate, DAO and endo- toxin were detected spectrophotographically. RESULTS: The level of D(-)-lactate was significantly high- er in the experimental group than that in the control group (P<0.01). Significant differences of D (-)-lactate levels were observed in Child-Pugh subgroups of the experimen- tal group (P <0. 01). The level of DAO was significantly higher in the experimental group than that in the control group (P <0.01), but the level of DAO in Child-Pugh sub- group C was significantly lower than that in Child-Pugh subgroup B (P<0.01). The level of endotoxin was signifi- cantly increased in the experimental group except Child Pugh subgroup A (P<0.01). The plasma levels of D(-) lactate, DAO and endotoxin were positively correlated with each other (P<0.01). CONCLUSIONS: The data suggest that both plasma D(-) lactate and DAO activity are sensitive markers for early diagnosis of gut failure and endotoxemia in patients with liver cirrhosis. The impairment of intestinal barrier func- tion may be one of the critical reasons for deterioration of liver cirrhosis. | Peng Ruan, Zuo-Jiong Gong and Quan-Rong Zhang Wuhan, China Department of Infectious Diseases, Renmin Hospital Wuhan University Medical College, Wuhan 430060 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，1： | 31 |
| 5 | Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway显示文摘Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA), acquire the ability to activate immune cells under inflammatory conditions. Here we inves- tigated whether HA fragments could activate Kupffer cells and analyzed the underlying mechanism. Kupffer cells were isolated from wild-type mice (WT, C3H/HeN) and TLR4 mutant mice (C3H/HeJ) and HA fragments were produced by the methods of enzyme digestion and chromatography. Then Kupffer cells were stimulated by HA fragments or other control stimuli. The activation of Kupffer cells was estimated as the release of pro-inflammatory cytokines. The activation of p38 MAPK pathway of Kupffer cells was checked and blocking experiments were done as well. The results indicated that HA fragments acquired the ability to activate Kupffer cells in vitro, which was TLR4 dependent and not due to contamination of lipopolysaccharide. Experiments of p38 MAPK kinase inhibition by SB-203580 verified p38 MAPK was required in HA fragments induced Kupffer cells activation. This suggests that HA fragments, degradative products of one of the major glycosaminoglycans of the extracellular matrix, play critical roles in Kupffer cell activation mediated by TLR4 signaling pathway, which is, at least partially, de- pendent on p38 MAPK activation. | ZHANG JinXiang1, WANG Hui2, XIAO Qing3, LIANG HuiFang4, LI ZhuoYa4, JIANG ChunFang1, WU HeShui5 & ZHENG QiChang5 1 Department of Emergency Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Medical Genetics, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 3 Department of Ophthalmology, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 4 Department of Medical Immunology, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 5 Department of General Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China | 2009 | Science China(Life Sciences)2009，52，2： | 33 |
| 6 | TLR4 signaling induced TLR2 expression in the process of mimic cerebral ischemia/reperfusion in vitro显示文摘Both TLR4 and TLR2 participated in the mediation of the inflammatory injury in the process of partial cerebral ischemia/reperfusion.However,it still remains unclear whether a crosstalk exists between TLR2 and TLR4 in ischemic cerebral damage.In the present study,we investigated the effect of TLR4 signaling on TLR2 expression during mimic cerebral I/R in vitro.BV-2 cells were cultured and treated with ischemia/reperfusion,then transfected with the plasmid pEGFP-H1/TLR4-siRNA,the plasmid pEGFP-H1/control sequence-siRNA and the blank plasmid,respectively.Interestingly,the expression of TLR2 and TLR4 mRNA and protein,NF-κB p65 mRNA and supernatant TNF-α level were significantly higher in ischemia/reperfusion treated cells than those lack of ischemia/reperfusion treatment,and as compared with those in ischemia/reperfusion treated cells without transfection,no significant differences about the above mentioned gene and protein expression were found in the blank plasmid tranfected cells and the plasmid pEGFP-H1/control sequence-siRNA transfected cells respectively,while the expression levels in the plasmid pEGFP-H1/TLR4-siRNA transfected cells were significantly lower.Additionally,in order to determine the effects of pyrrolidinediethyldithiocarbamate (PDTC),an NF-κB inhibitor,on the TLR4-induced TLR2 expression in BV-2 cells treated with ischemia/reperfusion,it was found that TLR4 and TLR2 mRNA expressions in PDTC pretreated cells were significantly lower in comparison with normal saline pretreated cells and non-pretreated cells.The data suggested that TLR2 activation,signaled by TLR4 and regulated by NF-κB,might be directly involved play an important role in ischemia/reperfusion induced brain damage. | SUN Peng1,ZHANG Qing2,HAN JiYuan1,TIAN Yuan3 & ZHANG JingHui3 1Department of Emergency,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China 2Department of Anesthesia,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China 3Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China | 2010 | Science China(Life Sciences)2010，53，2： | 30 |
| 7 | Edge Effect Correction in the S-A Method for Geochemical Anomaly Separation显示文摘Anomaly separation using geochemical data often involves operations in the frequency domain, such as filtering and reducing noise/signal ratios. Unfortunately, the abrupt edge truncation of an image along edges and holes (with missing data) often causes frequency distribution distortion in the frequency domain. For example, bright strips are commonly seen in frequency distribution when using a Fourier transform. Such edge effect distortion may affect information extraction results; sometimes severely, depending on the edge abruptness of the image. Traditionally, edge effects are reduced by smoothing the image boundary prior to applying a Fourier transform. Zero-padding is one of the most commonly used smoothing methods. This simple method can reduce the edge effect to some degree but still distorts the image in some cases. Moreover, due to the complexity of geoscience images, which can include irregular shapes and holes with missing data, zero-padding does not always give satisfactory results. This paper proposes the use of decay functions to handle edge effects when extracting information from geoscience images. As an application, this method has been used in a newly developed multifractal method (S-A) for separating geochemical anomalies from background patterns. A geochemical dataset chosen from a mineral district in Nova Scotia, Canada was used to validate the method. | Ge Yong Department of Earth and Atmospheric Science, York University, Toronto, ON, M3J 1P3, Canada State Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences & Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Cheng Qiuming Department of Earth and Atmospheric Science, York University, Toronto, ON, M3J 1P3, Canada Earth Systems and Mineral Resource Engineering Lab, China University of Geosciences, Wuhan 430074, China Zhang Shenyuan Department of Earth and Atmospheric Science, York University, Toronto, ON, M3J 1P3, Canada Department of Resource and Earth Science, China University of Mining & Technology, Beijing 100083, China | 2004 | Journal of China University of Geosciences2004，15，4： | 28 |
| 8 | Extended multivariate public key cryptosystems with secure encryption function显示文摘Advances in quantum computers pose great threats on the currently used public key cryptographic algorithms such as RSA and ECC. As a promising candidate secure against attackers equipped with quantum computational power, multivariate public key cryptosystems (MPKCs) have attracted increasing attention in recently years. Unfortunately, the existing MPKCs can only be used as a multivariate signature scheme, and it remains unknown how to construct an efficient MPKC enabling secure encryption. Furthermore, some multivariate signature schemes have been shown insecure in recent years, and it is also not trivial to build MPKC which can serve as a secure signature scheme. By employing the basic MQ-trapdoors, this paper proposes a novel MPKC and shows how it can be used as a multivariate signature scheme and a multivariate encryption scheme, respectively. The goal is achieved by incorporating our new hash authentication techniques and some modification methods such as the Shamir's minus method. Thorough analysis shows that our schemes are secure and efficient. Our MPKC gives a positive response to the challenges in multivariate public key cryptography. | WANG HouZhen 1,2 , ZHANG HuanGuo 1,2 , WANG ZhangYi 1,2 & TANG Ming 1,2 1 The Key Laboratory of Aerospace Information Security and Trusted Computing, Ministry of Education, School of Computer, Wuhan University, Wuhan 430079, China 2 State Key Laboratory of Software Engineering, Wuhan University, Wuhan 430072, China | 2011 | Science China(Information Sciences)2011，54，6： | 26 |
| 9 | Transforming growth factor-β1 in invasion and metastasis in colorectal cancer显示文摘AIM:To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and metastasis.METHODS:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent assay.TGFβ1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ hybridization.RESULTS:Serum levels of TGFβ1 in patients with colorectal cancer(40±18μg·L^-1)were significantly higher than those in the healthy control group(19±8μg·L^-1),P<0.05.Elevated levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg·L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei's stage(P<0.05),but there was no significant difference between,Duki's stage Bpatients and Dukei's stage Cpatients.In the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic lesion.Serum TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P<0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal cancer.It increased the invasive and metastatic potential of tumor by altering a tumor microenvironment.TGFβ1 may be used as a possible biomarke. | Bin Xiong Hong-Yin Yuan Ming-Bo Hu Feng Zhang Zheng-Zhuan Wei Ling-Ling Gong Guo-Liang Yang Department of Oncology,Affiliated Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 25 |
| 10 | Genetic analysis of 'all-fish' growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation显示文摘Recornbinant 'all-fish' growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant 'fast-growing' effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of 'fast-growing' transgenic fish. | WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,ZHU Zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China | 2001 | Chinese Science Bulletin2001，46，14： | 23 |
| 11 | Volcanism at the Permian-Triassic Boundary in South China and Its Effects on Mass Extinction显示文摘This paper discusses the clayrocks widespread at the Permian-Triassic boundary, which are mostly of volcanic origin. Volcanogenetic textures, structures and minerals such as high-temperature quartz are found in clayrocks at the Permian-Triassic boundary in many places. Thousands of microspherules have been collected from the Boundary clayrocks, many of which exhibit the typical features of the process from melting to cooling and solidification. indicating that they were formed by volcanic eruption or extraterrestrial impact. Volcanic effects on the Permian-Triassic mass extinction may be reflected in conodonts, algae and ammonoids. The Boundary clayrocks are found in many Permian-Triassic sections along the coast of Tethys. Their orighin remains to be studied. | Yin Hongfu, Huang Siji, Zhang Kexin, Yang Fengqing, Ding Meihua, Bi Xianmei and Zhang Suxin China University of Geosciences, Wuhan, Hubei | 1989 | Acta Geologica Sinica(English Edition)1989，63，4： | 22 |
| 12 | Presence of Antibodies to Heat Stress Proteins and its Possible Significance in Workers Exposed to High Temperature and Carbon Monoxide显示文摘Antibodies to the ubiquitous group of stress proteins known as heat shock proteins (Hsps) have been found to be associated with a number of diseases in humans. Hsps are known to be induced by certain xenobiotics, some of which are common in the working environment. The biological significance of the presence of such autoantibodies is presently unclear. In the present study, we used immunoblotting to investigate the presence of antibodies against the different stress proteins, Hsp27, Hsp60, Hsp71, Hse (heat shock cognate ) 73 and Hsp89a and D in groups of workers exposed to high temperature or carbon monoxide. These data were related to a detailed clinical evaluation and to various laboratory measurements including electrocardiogram (ECG), B echogram, white blood cell counts and typing, the activity of alanine aminotransferase (ALT), acid phosphatase (ACP) and alkaline phosphatase (ALP) and lymphocyte DNA damage. Antibodies to Hsp27 and Hsp71 were found more frequently in the high temperature and carbon monoxide-exposed groups than in controls (P (0.05 ). The carbon monoxide-exposed group showed the highest incidence of anti-Hsp antibodies. Anti-Hsp60 antibodies were only detected in workers exposed to high temperature or carbon monoxide. The percentage of workers with abnormal ECG, B echogram changes and displaying hepatitis B antigen (HBsAg ) was higher in the carbon monoxide group than in the control group (P<0.05 ).There was a significant inerease in the activity of ALT in the high temperature and carbon monoxide groups and in the activities of ACP and ALP in the carbon monoxide group (P<0.05 ). The extent of DNA damage measured in lymphoeytes was higher in workers from the high temperature and carbon monoxide-cxposed groups. We suggest that the increased frequeney of antibodies to Hsps is the result of these damages, of the release of denatured Hsps and of a decrease in the phagocytic ability of macrophages in these workers. The data gathered in the present study show a statistical relation between the occurrence of antibodies against Hsps and the frequency of health problems in workers and suggest a potential role for the antibodies as useful biomarkers to assess whether workers are experieneing environmental | WU TANG-CHUN TANGUAY, ROBERT M. WU YANG HE HAN-ZHEN XU DAI-GEN FENG JIA-DE SHI WU-XIANG AND ZHANG GUO-GAO (Institute of Occupational Medicine, Tongji Medical University, Wuhan 430040, China Centre de recherche du CHUL and Lab of Cellular and Develo | 1996 | Biomedical and Environmental Sciences1996，9，4： | 23 |
| 13 | Indian Ocean-MORB-type isotopic signature of Yushigou ophiolite in North Qilian Mountains and its implications显示文摘In order to explore the disputed issue concerning the tectonic affinity of the ancient ocean mantle of North Qilian Mountains (NQM), geochemical and Sr, Nd, Pb isotopic compositions of pillow basalts of the Yushigou Ophiolite (YSGO) suite from NQM have been analyzed systematically. The pillow basalts exhibit tholeiitic characteristics, with flat chondrite-normalized REE patterns ((La/Yb)N = 0.98―1.27). They display no Nb, Ta, Zr, Hf negative anomalies, and show MORB features in 2Nb-Zr/4-Y and Ti/100-Zr-Y×3 tectonic discrimination diagrams. These results indicate that the Yushigou ophiolite is most likely to be formed in a mid-ocean ridge or mature back-arc basin. Their isotopic data show a relatively broad and enriched 87Sr/86Sr (0.70509 ― 0.70700), restricted 143Nd/144Nd (0.512955―0.512978). Pb isotopes are in the range of 206Pb/204Pb (18.054―20.562), 207Pb/204Pb (15.537―15.743) and 208Pb/204Pb (38.068―38.530). These isotopic data imply that the basalts originated from the depleted mantle (DMM), with the involvement of enriched mantle compo-nents (mainly EMII). Geochemical comparisons between the basalts in YSGO and the MORB-type basalts of ophiolite suites occurring in the known ancient Tethyan tectonic domain indicate that the ancient oceanic mantle represented by YSGO suite forming in early Paleozoic in the North Qilian Moutains is very similar to the Tethyan mantle in both trace elements and isotopic compositions. The North Qilian Mountains should be a part of the Tethyan tectonic domain in early Paleozoic. This further implies that the Tethyan tectonic domain can be deduced to early Paleozoic in the study area, which will be helpful to discussing the tectonic affinity and evolution of the North Qilian Mountains. | HOU Qingye1,2, ZHAO Zhidan1,2, ZHANG Hongfei3, ZHANG Benren3 & CHEN Yuelong2 1. State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing 100083, China 2. School of Earth Science and Mineral Resources, China University of Geosciences, Beijing 100083, China 3. Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074, China | 2006 | Science China Earth Sciences2006，49，6： | 21 |
| 14 | Expression and signifi cance of TLR4 and HIF-1α in pancreatic ductal adenocarcinoma显示文摘AIM:To investigate the expression of toll-like receptor(TLR) 4,nuclear factor-κB(NF-κB) p65 and hypoxiainducible transcription factor 1α(HIF-1α) in pancreatic ductal adenocarcinoma and their clinical significance.METHODS:The mRNA of TLR4 and HIF-1α were investigated by real-time polymerase chain reaction in 30 cases of pancreatic ductal adenocarcinoma and its adjacent tissues,and expression of TLR4,NF-κB p65 and HIF-1α protein were detected by immunohistochemistry in 65 cases of pancreatic ductal adenocarcinoma tissues and 38 cases of corresponding adjacent tissues.The relationship between TLR4 or HIF-1α and pathologic features,as well as the association between TLR4 and HIF-1α,were also analyzed.Kaplan-Meier method was used to assess the impact of expression of TLR4 and HIF-1α on survival of patients with pancreatic cancer.RESULTS:The relative quantif ication of TLR4 and HIF-1α mRNA in tumor tissues was 0.81±0.10 and 0.87±0.11,respectively,signif icantly higher than that in adjacent tissues(0.81±0.10 vs 0.70±0.16,P=0.002;0.87±0.11 vs 0.68±0.13,P=0.000).The protein expression of TLR4,NF-κB p65 and HIF-1α in tumor tissues was 69.20%,66.15% and 70.80%,respectively,being signif icantly higher than that in adjacent normal tissues(69.20% vs 39.50%,P=0.003;66.15% vs 31.58%,P=0.001;70.80% vs 36.80%,P=0.001).There was no signif icant correlation between TLR4 or HIF-1α expression and the age,gender,tumor location,the degree of tumor differentiation in the patients(P>0.05).However,there was signif icant correlation between the expression of TLR4 or HIF-1α and tumor size,lymph node metastasis,venous invasion and clinical staging(P<0.05).The expression of TLR4 and HIF-1α had a signif icant impact on survival of patients with pancreatic adenocarcinoma.CONCLUSION:TLR4,NF-κB p65 and HIF-1α are overexpressed in pancreatic adenocarcinoma,TLR4 may be partly involved in up-regulating HIF-1α,and both synergestically promote development of pancreatic adenocarcinoma. | Jian-Jun Zhang,He-Shui Wu,Lin Wang,Yuan Tian,Jing-Hui Zhang,Hai-Long Wu Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Department of Pediatrics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Laboratory of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China | 2010 | World Journal of Gastroenterology2010，16，23： | 21 |
| 15 | Expression of TNF-related apoptosis-inducing Ligand receptors and antitumor tumor effects of TNF-related apoptosis-inducing Ligand in human hepatocellular carcinoma显示文摘AIM: To investigate the expression of TNF-related apoptosis -inducing Ligand (TRATL) receptors and antitumor effects of TRATL in hepatocellular carcinoma (HCC).METHODS: Expression of TRAIL receptors was determined in 60 HCC tissues, 20 normal liver samples and two HCC cell lines (HepG2 and SMMC-7721). The effects of TRAIL on promoting apoptosis in HCC cell lines were analyzed after the cells were exposed to the recombinant TRAIL protein,as well as transfected with TRAIL-expression construct. In vivo effects of TRAIL on tumor growth were investigated by using nude mice HCC model of hepG2.RESULTS: Both death receptors were expressed in all HCC tissues and normal hepatic samples. In contrast, 54 HCC tissues did not express DcR1 and 25 did not express DcR2.But both DcR were detectable in all of the normal liver tissues.The expression patterns of DR and DcR in HCC samples (higher DR expression level and lower DcR expression level) were quite different from those in normal tissue. DR5, DR4,and DcR2 expressed in both cell lines, while no DcR1 expression was detected. Recombinant TRAIL alone was found to have a slight activity as it killed a maximum of 15 % of HCC cells within 24 h. Transfection of the TRATL cDNA failed to induce extensive apoptosis in HCC lines. In vivo administration of TRAIL gene could not inhibit tumor growth in nude mice HCC model. However, chemotherapeutic agents or anticancer cytokines dramatically augmented TRAILinduced apoptosis in HCC cell lines.CONCLUSION: Loss of DcR (especially DcR1) in HCC may contribute to antitumor effects of TRAIL to HCC. HCC is insensitive towards TRAIL-mediated apoptosis, suggesting that the presence of mediators can inhibit the TRAIL celldeath-inducing pathway in HCC. TRAIL and chemotherapeutic agents or anticancer cytokines combination may be a novel strategy for the treatment of HCC. | Xiao-Ping Chen Song-Qing He Hai-Ping Wang Yong-Zhong Zhao Wan-Guang Zhang, Hepatic Surgery Center, Affiliated Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030, China | 2003 | World Journal of Gastroenterology2003，9，11： | 21 |
| 16 | Geochemistry of high-Mg andesites from the early Cretaceous Yixian Formation, western Liaoning: Implications for lower crustal delamination and Sr/Y variations显示文摘85 volcanic rocks of the Yixian Formation from the Sihetun type section were collected and analyzed for geochemical and isotopic compositions. Major element compositions indicate that the Sihetun volcanic rocks are high magnesium andesites with some basalts occurring at the bottom of section. The Sihetun high magnesium andesites (SiO2 = 52.82―59.31wt%, Al2O3 = 14.15―16.35wt%) show many characteristics of adakites such as depletion in heavy rare-earth elements (HREE; Yb = 1.03―1.88 μg/g) and Y(12―20 μg/g) and high Sr (620―1323 μg/g) and Sr/Y(32―88), with high LaN/YbN ratio (10―25). They share similar major and trace element characteristics to volcanic rocks from the Xinglonggou Formation except their lower Nd isotope ratios (143Nd/144Nd (130Ma) = 0.5118―0.5119, ε Nd (130Ma) = ?11.6―?13.8, 87Sr/86Sr (130 Ma) = 0.7058―0.7064. They were in- terpreted in a way that eclogite that formed at the base of thickened Archean lower crust of the North China craton foundered into the convecting mantle and subsequently melted and interacted with peridotite. However, compared to the Xinglonggou volcanic rocks, the source of the Sihetun magma contained more ancient continental crustal material in order to explain its evolved Nd isotopes. The age of the Sihetun Formation was 120 to 130 Ma, and this indicates that delamination lasted to the early Cretaceous period. The Sr contents and Sr/Y ratios of the Sihetun high-Mg andesites show sig- nificant negative correlations with SiO2 for samples with SiO2 > 56%. These suggest that the Sr and Sr/Y values were reduced due to fractional crystallization of plagioclase. Accordingly, the effect of crystallization on volcanic Sr and Sr/Y ratio has to be taken into account. | WANG Xiaorui1, GAO Shan1,2, LIU Xiaoming1, YUAN Honglin1, HU Zhaochu1, ZHANG Hong1,3 & WANG Xuance4 1. State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi`an 710069, China 2. State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Wuhan 430074, China 3. Shenyang Institute of Geology and Mineral Resources, Shenyang 110032, China 4. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China | 2006 | Science China Earth Sciences2006，49，9： | 19 |
| 17 | NONLINEAR STABILITY OF NATURAL RUNGE-KUTTA METHODS FOR NEUTRAL DELAY DIFFERENTIAL EQUATIONS显示文摘This paper first presents the stability analysis of theoretical solutions for a class of nonlinear neutral delay-differential equations (NDDEs). Then the numerical analogous results, of the natural Runge-Kutta (NRK) methods for the same class of nonlinear NDDEs, are given. In particular, it is shown that the (k, l)-algebraic stability of a RK method for ODEs implies the generalized asymptotic stability and the global stability of the induced NRK method. | Cheng-jian Zhang(Department of Mathematics, Huazhong University of Science and Technology, Wuhan 430074, Hubei, P. R. China) | 2002 | Journal of Computational Mathematics2002，20，6： | 18 |
| 18 | Late Caledonian Ductile Thrusting Deformation in the Central East Kunlun Belt, Qinghai, China and Its Significance: Evidence from Geochronology显示文摘A high-angle ductile thrusting deformation with top-to-the-north movement penetratively developed in the Proterozoic-Early Paleozoic metamorphic rocks along the Central East Kunlun beR. The deformed rocks suffered epidote-amphibolite facies metamorphism. On the basis of our previous study, we present more data in this paper to further support that the ductile thrust deformation occurred in the later Caledonian and more detailed information about the deformation.A zircon U-Pb concordant age of 446±2.2 Ma of a deformed granodiorite in the ductile thrust zone was obtained and can be interpreted as the lower limit of the deformation. A syntectonically crystallized and also strongly deformed hornblende Ar/Ar dating gives an Ar/Ar plateau age of 426.5±3.8 Ma, which represents the deformation age. A strongly orientated muscovite gives an Ar/Ar plateau age of 408±1.6Ma, representing the cooling age after the peak temperature, constraining the upper limit of the ductile thrust deformation. This ductile thrust deformation can be interpreted as the result of the closing of the Central East Kunlun archipelago ocean. To the north, At/At plateau ages of 382.9±0.2 Ma and 386.8±0.8 Ma of muscovite in the deformed Xiaomiao Group represent the uplift cooling ages of deeper rocks after the thrusting movement. The original thrusting foliation has a low angle. A rotation model was put forward to explain the development of the foliation from the original low-angle to present high-angle dipping. | WANG Guocan, CHEN Nengsong, ZHU Yunhai and ZHANG Kexin Faculty of Earth Sciences, China University of Geosciences, Wuhan, Hubei 430074 E-mail: wgcan@cug.edu.cn Ren Xifei, Liu Ruixun and Xie Guanglian | 2003 | Acta Geologica Sinica(English Edition)2003，77，3： | 18 |
| 19 | Petrology, Metamorphic Process and Genesis of The Dabie--Sulu Eclogite Belt, Eastern--Central China显示文摘The Qinling-Dabie-Sulu high-pressure and ultra-high pressure metamorphic belt wasformed by subduction and collision between the North China and Yangtze plates. The study ofthe eclogite belt is very important in understanding the evolution of the Qinling Dabie orogen. Inthe present paper the geology, petrology, minerology and chronology of the eclogites in the Dabieand Sulu areas are described. The principal conclusions of this work are as follows: (1) Based up-on the field occurrence and the P-T conditions of the eclogites, two types of eclogite can be dis-tinguished: Type 1—the low-temperature and high-pressure eclogite in the mid-late Proterozoicmetamorphic series, and Type 2—the ultra-high pressure eclogite in the late Archaean to earlyProterozoic metamorphic complex. In the Dabie area, the ultra-high-pressure eclogite,high-pressure eclogite and epidote-blueschist units are nearly parallel to each other and stretchintermittently from north to south. (2) The P-T conditions of the high-pressure eclogites and ul-tra-high pressure eclogites have been estimated. The former are formed at 450-550℃ and1.4-1.6 GPa; while the latter at 650-870℃ and >2.7-2.9 GPa in the Dabie area and at820-1000℃ and >2.8-3.1 GPa in the Sulu area. The metamorphic temperatures of the eclogitesincrease progressively from west to east. (3) The ultra-high pressure eclogites were subjected to 5stages of metamorphism: pre-eclogite epidote amphibolite facies, peak coesite eclogite facies,post-eclogite amphibolite facies, epidote-blueschist facies or epidote amphibolite facies andgreenschist facies. The general features of the PTt path of the ultra-high pressure eclogite are:clockwise pattern, progressive metamorphism being a process of slow increasing temperature andrapid increasing pressure, and the retrogressive section with nearly isothermal decompression atthe early stage, isobaric cooling at the middle stage and nearly isothermal decompression at thelate stage. (4) At least two stages of high-pressure metamorphism occurred in the orogenic belt:the high-pressure eclogite and ultra-high pressure eclogite were formed by the subduction of theoceanic crust northward beneath the North China plate or the Dabie block during theCaledonian; while the epidote-blueschist belt came into being by subdution and collision be-tween the two continental plates during the Indosinian. (5) Due to the continuous sequentialsubduction of the cold plate, the ultra high-presssure metamorphic rocks were uplifted to thecrust by the underplating processes. They can be preserved just because of the 'frozen effect' re-sulting from the continuous subduction of the cold plate. (6) The carbonates, such as magnesite,breunnerite, aragonite and dolomite, and the H2O-bearing minerals, such as phengite, epidoteand zoisite, were stable during the high-pressure and/or ultra-high pressure metamorphism. | Zhang Zeming You Zhendong Han Yujing and Sang Longkang Department of Earth Sciences, China University of Geosciences, Wuhan, Hubei | 1996 | Acta Geologica Sinica(English Edition)1996，70，2： | 17 |
| 20 | Interactions between hotspots and the Southwest Indian Ridge during the last 90 Ma:Implications on the formation of oceanic plateaus and intra-plate seamounts显示文摘This study investigates the relationship between the hotspot-ridge interaction and the formation of oceanic plateaus and seamounts in the Southwest Indian Ocean.We first calculated the relative distance between the Southwest Indian Ridge (SWIR) and relevant hotspots on the basis of models of plate reconstruction,and then calculated the corresponding excess magmatic anomalies of the hotspots on the basis of residual bathymetry and Airy isostasy.The results reveal that the activities of the Marion hotspot can be divided into three main phases:interaction with the paleo-Rodrigues triple junction (73.6-68.5 Ma),interaction with the SWIR (68.5-42.7 Ma),and intra-plate volcanism (42.7-0 Ma).These three phases correspond to the formation of the eastern,central,and western parts of the Del Cano Rise,respectively.The magnitude and apparent periodicity of the magmatic volume flux of the Marion hotspot appear to be dominated by the hotspot-ridge distance.The periodicity of the Marion hotspot is about 25 Ma,which is much longer than that of the Hawaii and Iceland hotspots (about 15 Ma). | ZHANG Tao 1,2,3,4,LIN Jian 5 & GAO JinYao 3,4 1 Institute of Geodesy and Geophysics,Chinese Academy of Sciences,Wuhan 430077,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 3 The Second Institute of Oceanography,State Oceanic Administrator,Hangzhou 310012,China 4 Key Laboratory of Submarine Geosciences,State Oceanic Administrator,Hangzhou 310012,China 5 Department of Geology and Geophysics,Woods Hole Oceanographic Institution,Woods Hole,MA 02543,USA | 2011 | Science China Earth Sciences2011，54，8： | 16 |
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