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| 1 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 2 | 深部地球条件下铁和氧元素的化学变化显示文摘氧与铁分别为地球上丰度最高的元素与占地球质量最大的元素,它们所形成的化合物遍布我们的星球,具有-2价的氧控制着地球的氧逸度,随着深度的增加氧逸度逐渐降低,与铁所形成的氧化物中铁的价态也逐渐降低,O/Fe逐渐增加,近些年来科学家们发现深部地球条件下铁的一系列氧化物,其中具有高压黄铁矿结构的铁超氧化物FeO2具有最高的O/Fe,并且它还能够携带一定量的H(表示为Py-FeO2H,, x=0-1)。 | 夏群科 Liu J Hu Q Y Bi W L Yang L X Xiao Y M Chow P Meng Y.Prakapenka V B Mao H K Mao W L | 2019 | 矿物岩石地球化学通报2019，38，3： | 2 |
| 3 | Growth and Characterization of AlGaN/GaN Heterostructure Using Unintentionally Doped AlN/GaN Superlattices As Barrier Layer显示文摘 | Zhang M L Wang X L Xiao H L | 2009 | Superlattices and Microstructures2009，45，： | 1 |
| 4 | Effect of Temperature on reduction of CaSO4 oxygen carrier in chemical- looping combustion of simulated coal gas in a fluidized bed reactor显示文摘 | SONG Q XIAO R DENG Z SHEN L XIAO J ZHANG M | 2008 | Ind Eng Chem Res2008，47，21： | 1 |
| 5 | Genetic polymorphisms of MDM2 and TP53 genes are associated with risk of nasopharyngeal carcinoma in a Chinese population显示文摘 | Xiao M Zhang L Zhu X | 2010 | BMC Cancer2010，10，： | 1 |
| 6 | An approximate analytic solution of strongly pumped Yb-doped double-clad fiber lasers without neglecting the scattering loss显示文摘 | Xiao L Yan P Gong M | 2004 | Opt Commun2004，230，： | 1 |
| 7 | Heavy meted contamination and its environmental risk assessment in surface sediments form Lake Dongting, Peo- ple's Republic of China 显示文摘 | Qian Y Zheng M H Gao L Zhang B Liu W Jiao W Zhao X Xiao K | 2005 | Bull Environ Contain Toxicol2005，75，1： | 1 |
| 8 | Biodiesel from palm oil via loading KF/Ca-A1 hydrotalcite catalyst显示文摘 | Gao L J Teng G Y Xiao G M | 2010 | Biomass and Bioenergy2010，34，： | 1 |
| 9 | Immunochemical analysis of human papillomavirus L1 capsid protein in Liquid-based cytology samples from cervical lesions显示文摘 | Xiao W Bian M Ma L | 2012 | Acta Cytol2012，54，5： | 1 |
| 10 | 显示文摘 | Gao L Xiao M Wang S | 2008 | Journal of Applied Polymer Science2008，108，2： | 1 |
| 11 | 查看详情显示文摘 | Wu X L Meng Y Z Xiao M Lu Y X | | 0，，： | 1 |
| 12 | Preparation and characterization of CdS nanoparticles decorated into titanate nanotubes and their photocatalytic properties显示文摘 | Xiao M W Wang L S Wu Y D | | 0，，： | 1 |
| 13 | Rough prime ideals and rough fuzzy prime ideals in semigroups显示文摘 | Xiao Q M Zhang Z L | 2006 | Information Sci- ences2006，176，： | 1 |
| 14 | Nitrogen transfer of fuel-N in chemical looping combustion 显示文摘 | Song T Shen L H Xiao J Chen D Q Gu H M Zhang S W | 2012 | Combustion and Flame2012，159，3： | 1 |
| 15 | Synthesis and properties of polystyrene/graphite nanocomposites显示文摘 | Xiao M Sun L Liu J | 2002 | Polymer2002，43，： | 1 |
| 16 | Internet QoS: the Big Picture显示文摘 | Xiao X P Ni L M | 1999 | IEEE Network Magazine1999，13，2： | 1 |
| 17 | A Model for Stochastic Acceleration of Electrons During Geomagnetic Storms显示文摘 | Thorne R M Xiao F L | 2000 | Adv Space Res2000，25，12： | 1 |
| 18 | Tip in Situ Chemical Modification and its Effects on Tribological Measurements 显示文摘 | Qian L M Xiao X D Wen S Z | 2000 | Langmuir2000，16，： | 1 |
| 19 | Internet QoS:A Big Picture 显示文摘 | XIAO Xipeng NI L M | 1999 | IEEE Network Magazine1999，13，2： | 1 |
| 20 | Reduction of CaSO4 oxygen carrier with coal in chemical looping combustion: Effects of temperature and gasification intermediate显示文摘 | ZHENG M SHEN L XIAO J | 2010 | Int J Greenhouse Gas Control2010，4，6： | 1 |
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