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| 1 | Association of H.pylori infection with gastric carcinoma:a Meta analysis显示文摘AIM: To follow the principles of evidence based medicine to reach the integrated results of these studies.METHODS: Twenty-one papers of case-control studies were selected, including 11 on gastric cancer, 7 on precancerous lesion of stomach and 3 on lymphoma of stomach: Meta analysis was used to sum up the odds ratios (OR) of these studies.RESULTS: H. Pylori vsgastric cancer (intestinal and diffuse type): the odds ratio from the fixed effect model is 3.0016(95% Cl 2.4197-3.7234, P < 0.001 ). H. Pylori vs precancerous lesion of stomach: a random effect model was used to calculate the summary odds ratio and its value is 2.5635 (95% Cl: 1.8477-3.5566, P < 0.01). H. Pylori vs lymphoma of stomach: though the quantity of literature is too small to make Meta analysis, the data of these 3 studies show that lymphoma of stomach is highly associated with H. Pylori infections.CONCLUSION: Since it had been revealed that H. Pylori infection pre-exists in gastric carcinoma and precancerous lesions, the results of Meta analysis present a strong evidence to support the conclusion that H. Pylori infection is a risk factor for gastric carcinoma. | Fu-Bo Xue~1 Yong-Yong Xu~1 Yi Wan~1 Bo-Rong Pan~2 Jun Ren~2 Dai-Ming Fan~3 1 Department of Health Statistics,Department of2 Oncology3 Gastroenterology of XiJing Hospital,the Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 66 |
| 2 | Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with the X protein of hepatitis B virus显示文摘AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-KB (NF-κB) and the expression of NF-KB inhuman hepatocellular carcinoma tissues.METHODS Immunohistochemistry SP methodwas used to detect the expression of NF-κB and the X protein of HBV in human hepatocellularcarcinoma tissues of 52 cases. Gene transfectionmediated by lipofectamine was used to transfectthe eukaryotic expression vector pCDNA3. 1-HBXof HBV x gene into human hepatocellularcarcinoma cell line HCC-9204 and NF-κB wasdetected.RESULTS NF-κB was widely expressed inhuman hepatocellular carcinoma tissues in atotal of 52 cases and its expression was relatedto the X protein of HBV. NF-KB was localizedboth in the cytoplasm and . The nuclei ofhepatocellular carcinoma cells in 11 cases whichwere positive for the X protein of HBV while in 41cases negative for the X protein of HBV, NF-Kbwas only localized in the cytoplasm ofhepatocellular carcinoma cells but translocatedto the nuclei of hepatocellular carcinoma cellsafter the eukaryotic expression vectorpCDNA3.1-HBX was transfected into HCC-9204cells.CONCLUSION This study strongly suggeststhat the nuclear factor NF-KB is widely expressedin hepatocellular carcinoma tissues in differentstyles according to the expression of the Xprotein of HBV. NF-κB is abnormally activated inhepatocellular carcinoma, which is probablyrelated to the X protein of HBV. The X protein ofHBV can activate NF-κB to translocate into nucleiof hepatocellular carcinoma cells. | Shuang Ping Guo~1 Wen Liang Wang~1 Yu Qiang Zhai~2 Yi Ling Zhao~1 ~1Department of Pathology,Xijing Hospital of the Fourth Military Medical University,Xi’an,China ~2Department of Urology,the Central Hospital of Xi’an,China | 2001 | World Journal of Gastroenterology2001，7，3： | 55 |
| 3 | Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma显示文摘AIM To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesisof human gastric carcinoma more directly.METHODS The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF165 complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or downregulated.RESULTS VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR,localized in both the cytoplasm and membrane.Introduction of VEGF165 antisense into human gastric cancer cells ( SGC-7901, immunofluorescence intensity,31.6%)) resulted in a significant reduction in VEGFspecific messenger RNA and total and cell surface VEGF protein ( immunofluorescence intensity, 8.9%)(P<0.05). Conversely, stable integration of VEGF165 in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity,75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tomor volume: 345.40 ± 136.31 mm3) (P<0.05 vs control SGC7901 group: 1534.40 ± 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tomor volume: 2350.50 ± 637.70mm3) (P<0.05 vs control SGC-7901 group).CONCLUSION This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer. | Du-Hu Liu Xue-Yong Zhang Dai-Ming Fan Yu-Xin Huang Jin-Shan Zhang Wei-Quan Huang Yuan-Qiang Zhang Qing-Sheng Huang Wen-Yu Ma Yu-Bo Chai Ming Jin Institute of Digestive Disease,Xijing Hospital,~2 Department of Gastroenterology,Tangdu Hospital,~3Department of Histology and Embryology,~4 Department of Microbiology,~5 Department of Biochemistry,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 37 |
| 4 | Function of microglia and macrophages in secondary damage after spinal cord injury显示文摘Spinal cord injury(SCI) is a devastating type of neurological trauma with limited therapeutic opportunities.The pathophysiology of SCI involves primary and secondary mechanisms of injury.Among all the secondary injury mechanisms,the inflammatory response is the major contributor and results in expansion of the lesion and further loss of neurologic function.Meanwhile,the inflammation directly and indirectly dominates the outcomes of SCI,including not only pain and motor dysfunction,but also preventingneuronal regeneration.Microglia and macrophages play very important roles in secondary injury.Microglia reside in spinal parenchyma and survey the microenvironment through the signals of injury or infection.Macrophages are derived from monocytes recruited to injured sites from the peripheral circulation.Activated resident microglia and monocyte-derived macrophages induce and magnify immune and inflammatory responses not only by means of their secretory moleculesand phagocytosis,but also through their influence on astrocytes,oligodendrocytes and demyelination.In this review,we focus on the roles of microglia and macrophages in secondary injury and how they contribute to the sequelae of SCI. | Xiang Zhou Xijing He Yi Ren | 2014 | Neural Regeneration Research2014，9，20： | 31 |
| 5 | Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats显示文摘AIM: To invsstigare the effect of L-NAME on nitric oxide andgastriubtestubal motility alterations in cirrhotic ratsMETHODS: Rats with cirrhosis induced by carbontetrachloride were randomly divided into two groups, one( n= 13) receiving 0. 5 mg@ kg-1 per clay of NG-nitro-L-argininemethyl ester (L-NAME), a nitric oxide synthase inhibitor,for 10 days, whereas the other group ( n = 13) and control( n = 10) rats were administrated the same volume of 9 g@ L-1saline.Half gastric emptying time and 2 h residual rate weremeasured by SPECT, using 99m Tc-DTPA-labeled bariumsuifate as test meal. Gastrointestinal transition time wasrecorded simultaneously. Serum concentration of nitrcoxide (NO) was determined by the kinetic cadmiunreduction and colorimetric methods. ImmunohistochemicalSABC method was used to observe the expression anddistribution of three types of nitric oxide synthase (NOS)isoforms in the mt gastrointestinal tract. Western blot wasused to detect expression of gastrointestinal NOS isoforms.RESULTS: Half gastric emptying time and trans-gastrointestinal time were significantly prolonged( 124.0 ± 26.4min; 33.7± 8.9min;72.1 ± 15.3 min; P<0.01), (12.4±0.5h; 9.5±0.3 h; 8.2±0.8 h; P<0.01), 2h residual rate wasraised in cirrhotic rots than in controls and cirrhotic ratstreated with L-NAME(54.9± 7.6 % ,13.7 ± 3.2 %, 34.9± 10.3%, P< 0.01). Serum concentration of NO was significantlyincreased in cirrhotic rots than in the other groups (8.20 ± 2.48)μmol@L-1, (5.94± 1.07) μmol@L-1 ,and control (5.66± 1.60) tμmol@L-1, P< 0.01. NOS staining intensities which weremainly located in the gastrointestinal tissues were markedlylower in cirrhotic rats than in the controls and cirrhotic ratsafter treated with L- NAME.CONCLUSION: Gastrointestinal motility was remarkablyinhibited in cirrhotic rats, which could he alleviated by L-NAME. Nitric oxide may play an important role in theinhibition of gastrointestinal motility in cirrhotic rats. | Xin Wang Zong-You Zhang Mei Lan Ji-Yan Miao Xue-Gang Guo Yong-Quan Shi Yan-Qiu Zhao Jie Ding Kai-Cun Wu Dai-Ming Fan,Institute of Digestive disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Yue-Xia Zhong,Emergency Department,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shaanxi Province,China Ju Lu,Class EE 87,Department of Electronic Engineering,Tsinghua University,Beijing 100084,China Bo-Rong Pan,Oncology Center,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 19 |
| 6 | Current gene therapy for stomach carcinoma显示文摘Gastric cancer is common in China [1-42],and its early diagnosis and treatment in advanced stage are difficult [31-50].In recent years ,gene study in cancer is a hotspot ,and great progress has been achieved [41-80] .Cancer gene therapy has shifted from the imagination into the laboratory and clinical trials. | Chang-Tai Xu~1 Lian-Tian Huang~1 Bo-Rong Pan~2 1 Editorial Department,the Journal of Fourth Military Medical University2 Oncology Center,Xijing Hospital,Fourth Military Medical University,169 Changle Xilu,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 16 |
| 7 | Pharmacokinetics of traditional Chinese syndrome and recipe:a hypothesis and its verification(Ⅰ)显示文摘AIM To propose a hypothesis defining theabsorption,distribution,metabolism andelimination of traditional Chinese recipe(TCR)-component in blood of healthy subjects andpatients,and estimate its correctness.METHODS The pharmacokinetics(PK)of samedose of drug was studied in the animal model oftraditional Chinese syndrome(S)and healthyanimals.The classification,terminology,concept and significance of the hypothesis wereset forth with evidence provided in the presentstudy.The hypotheses consisted of traditionalChinese syndrome PK(S-PK)and traditionalChinese recipe PK(R-PK).Firstly,the observedtetramethylpyrazine(TMP)PK in healthy,chronically reserpinized rats(rat model ofspleen deficiency syndrome,RMSDS)andRMSDS treated with Sijunzi decoction(SJZD)forconfirmation were used to verify S-PK; secondly,the ferulic acid(FA)PK in healthy andhigh molecular weight dextran(HMWD)-inducedrabbit model with blood stasis syndrome(RDBSS)was also used to verify S-PK;andlastly,TMP PK parameters in serum of healthyrats after orally taken Ligusticum wallichii(LW),LW and Salvia miltiorrhiza(LW&SM)decoctions were compared to verify R-PK.RESULTS The apparent first-order absorption[Ka,(13.61±2.56)h-1,area under the blooddrug concentration-time curve[AUC,(24.88±9.76)μg.h-1mL-1],maximum drug concentration[Cmax,(4.82±1.23)μg·mL-1]of serum TMP inRMSDS were increased markedly(P<0.05)compared with those[Ka=(5.41±1.91)h-1,AUC=(5.20±2.57)μg·h-1·mL-1,Cmax=(2.33±1.77)μg·mL-1]of healthy rats(HR).Theapparent first-order rate constant for α and βdistribution phase[α=(0.38±0.09)h-1,β=(0.06±0.03)h-1,the apparent first-orderintercompartmental transfer rate constants[K10=(0.24±0.07)h-1,K12=(0.11±0.02)h-1,K21=(0.11±0.02)h-1]of serum TMP in RMSDS weredecreased significantly(P<0.01)comparedwith those[K10=(0.88±0.20)h-1,K12=(1.45±0.47)h-1,K21=(0.72±0.22)h-1]of HR.However,no apparent differences occurredbetween HR and RMSDS treated with SJZD.Theserum FA concentration and its AUC[(5.6690±2.3541)μg·h-1·mL-1] in RMBSS were also higherthan those[AUC=(2.7566±0.8232)μg·h-1·mL-1]of healthy rabbits(P<0.05).The Ka(11.51±2.82)h-1,AUC(0.84±0.17)μg·h-1·mL-1of LW & SM-derived TMP in serum weremuch lower(P<0.05)than those[Ka=(19.58±4.14)h-1,AUC=(1.27±0.26)μg·h-1·mL-1]ofLW-derived TMP in serum after oral decoctions.CONCLUSION The SDS and blood stasissyndrome state could affect significantly thepharmacokinetic parameters of drugs and theabnormal SDS pharmacokinetic parameters couldbe normalized by SJZD.The combination ofChinese medicine in TCR could reciprocallyaffect the pharmacokinetic parameters of othercomponents absorbed into the systemiccirculation.These results support the S-and R-PK hypothesis. | Xi Huang Ping Ren Ai Dong Wen Li Li Wang Li Zhang Feng Gao Laboratory of Clinical Pharmacology of Chinese Medicine,Xijing Hospital,The Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Department of Pharmacy,Xijing Hospital,The Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Department of Physiology,The Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 16 |
| 8 | Matrine reduces the proliferation and invasion of colorectal cancer cells via reducing the activity of p38 signaling pathway显示文摘Matrine 被使用了在反煽动性并且反癌症治疗很长时间。然而,在 colorectal 癌症(CRC ) 的反变形的效果和相关机制仍然是不清楚的。在这研究,我们调查了 matrine 的管理是否能经由调整表明小径的 p38 禁止人的 CRC 房间的增长,活动性,和侵略。结果证明 matrine 在 vitro 并且在 vivo 禁止了 CRC 房间的移植和侵略。在为 24 h 与 matrine 被对待以后,另外,表示在 CRC 房间矩阵 metalloproteinase-2 (MMP-2 ) 和 MMP-9 以及朊酶活动铺平以一种剂量依赖者方式被减少。而且, matrine 显然减少了 p38 的 phosphorylation 水平。有 p38 禁止者(SB203580 ) 和 matrine 的联合治疗在 CRC 房间导致了侵略以及 MMP-2/-9 表示的 synergistic 减小。matrine 在 vivo 禁止了 CRC 肿瘤的增长和转移,这也被发现。在结论, p38 发信号小径可以由减少 MMP-2/-9 的表示在 CRC 房间的移植和侵略上在 matrine 的禁止的效果包含,建议 matrine 可以是为 CRC 的一个潜在的治疗学的代理人。 | Hongtao Ren Shuqun Zhang Hongbing Ma Yali Wang Di Liu Xijing Wang Zhongwei Wang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，12： | 16 |
| 9 | Epidemiological survey of cryptosporidiosis in Anhui Province China显示文摘AIM: To provide scientific evidence for prevention and controlling of cryptosporidiosis, the infection of Cryptosporidium parvum and its epidemiological characteristics were studied in some areas of Anhui Province.METHODS: The oocyst of Cryptosporidium parvum in 5421fresh stool samples from eleven areas of Anhui Provincewas tested by auramine-phenol stain and improved anti-acidstain respectively. The specific antibody of IgG, IgM and Tsubsets of 41 patients with positive Cryptosporidium parvumin stools were detected by ELISA and biotin-streptavidin(BSA) respectively.RESULTS: The total infective rate of Cryptosporidiumparvum was 1.33 % (74/5421). Among them, the positiverates of oocyst in the areas of Huaibei (1. 82 % ) and Fuyang( 1. 80 % ) were higher. The positive rates of oocyst in stoolsof infants, pupils, middle school students, collegestudents, adults, patients with diarrhea, and those withimmunodeficiency were 3.15 % (28/889), 0.82 % (9/1098), 0.82 %(9/1092), 0.83 %(8/969), 0.85 % (9/1095), 2.88 %(8/27g) and 8.33 %(3/36) % respectively. The positive rates ofoocyst in infants and the patients with diarrhea andimmunodeficiency were significantly higher than those incontrols ( P < 0.01 ). The positive rate of oocyst in maleswas similar to that in females ( P> 0.05). The positive rateof oocyst in urban areas ( 1. 13 %) was significantly lowerthan those in rural areas ( 1. 72 %, P < 0.01 ). The positiverates of specific IgG, IgM and IgG + IgM in sera of thepatients with positive oocyst in stool were 63.4 % ( 26/41 ),17.1% (7/41), 19.5 % (8/41) respectively. The numberfractions of T subsets of CD3 + , CD4 + , CD8 + and CD4 +/CD8 + of the patients were 0.66 ± 0.07, 0.44 ± 0.06, 0.28 ± 0.04 and 1. 58 ± 0.32 respectively. The difference between thepatients and the controls was significant ( P < 0. 05). Themain manifestations of the patients were subclinicalinfection, in forms of slight abdominal pain, mild diarrhea,and loose stool.CONCLUSION: There are two infection peaks in infection ofCryptosporidium parvum and its infection can he foundmore often in infants, patients with diarrhea orimmunodeficiency, and in rural areas. Subclinical infectionis the main manifestation and might he easily misdiagnosed.When the therapeutic effectiveness is low for diarrhea, theinfection of Cryptosporidium parvum should he considered,conceming their age and immune function. | Ke-Xia Wang Chao-Pin Li Jian Wang,Department of Aetiology and Immunology, School of Medicine, Huainan University of Technology,Huainan 232001,Anhui Province,China Bo-Rong Pan,Department of Oncology,Xijing Hospital,the Fourth Military Medical University,Xian,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 15 |
| 10 | Function and regulation of cholecystokinin-octapeptide, β-endorphin and gastrin in anorexic infantile rats treated with ErBao Granules显示文摘AIM To study the role of cholecystokinin octapeptide ( CCK-8), β-endorphin ( β-EP), and gastrin in an anorexic infantile rat model and no subsequent regulation of nose peptides by the Yunpi complex prescription ErBao Granule.METHODS We fed infantile rats with special prepared forage. A liquid extract of ErBao Granule was administered to the rats daily for 3weeks, CCK-8, β-EP, and gastrin concentrations in hypothalamus, gastric antrum, and plasma of the rats were measured by radioimmunoassay,and were compared with controls.RESULTS Treatment of rats with ErBao Granule inhibited CCK-8 secretion and increased β-EP and gastrin secretion. CCK-8 concentration in hypothalamus and plasma of model control group increased significantly and correlated negatively with food intake of models.respectively. β-EP concentration in gastric antrum and plasma of model control group decreased significantly and showed a positive correlation with food intake of models,respectively. Hypothalamus concentration of β-EP was similar in models and controls. Gastrin concentration in gastric antrum of models was lower than in the blank control group, and correlated positively to food intake of models.Finally, CCK-8 concentrations in plasma of rats showed a positive correlation with plasma β-EP(r- 0.68, P<0.05).CONCLUSION The increased plasma and hypothalamus concentration of CCK-8,decreased gastric antrum and plasma level of β-EP. and decreased gastric antrum concentration of gastrin are associated significantly with the anorexia of infantile anorexic rat models produced by special forage. ErBao Granule can reverse these changes, which may be the major mechanisms of ErBao Granule simulating feeding. | Yong Ping Du1 Yue Ping Zhang2 Shou Chuan Wang3 Jian Shi1 Shao Hua Wu1,1.Department of Traditional Chinese Medicine. Xijing Hospital, theFourth Military Medical University. Xi an 7101132, Shaanxi Province,China 2.Department of Pediatrics. Xijing Hospital, the Fourth Military MedicalUniversity. Xi’an 710032. Shaanxi Province. China 3.Department of Pediatrics. Nanjing University of Traditional ChineseMedicine, Nanjing71011?l, Jiangsu Province. China | 2001 | World Journal of Gastroenterology2001，7，2： | 14 |
| 11 | Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3显示文摘AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas.METHODS: mRNA was isolated from the hybridoma cell lineproducing MC3 and the DNAs encoding variable domains ofheavy and light chains(VH and VL) oftthe antibody wereamplified separately byRT-PCR and assembled into ScFvDNA with a linker DNAThe ScFv DNA was iigated into thephagemid vector pCANTAB5E and the ligated sample wastransformed into E. coil TG1. The transformed cells wereinfected with M13KO7 helper phage to yield recombinantphages. After two rounds of panning with gastric carcinomacell line AGS highly expressing MC3-binding antigen, thephage clones displaying ScFv fragments of the antibodywere selected by ELISA. 4 phage clones showing strongsignal in ELISA were used to infect E. coil HB2151 toexpress soluble ScFvs. The soluble ScFve were identified byDot blot and Western blot, and their antigen-binding activitywas assayed by ELISA. The VH and VL DNAs of the ScFvDNA derived from phage clone 19 were sequenced.RESULTS: The VH, VL and ScFv DNAs were about 340 bp,320 bp and 750 bp respectively. After two rounds of panningto the recombinant phages, 18 antigen-positive phageclones were selected from 30 preselected phage clones byELISA. All the soluble ScFvs derived from the 4 out of the 18antigen-positive phage clones were about Mr 32 000 andconcentrated in periplasmatic space under the given culturecondition. The soluble ScFvs could bind the antigen, andthey shared the same binding site with MC3. The sequencesof the VH and VL DNAs of the MC3 ScFv showed that thevariable antibody genes belonged to the IgG1 subgroup,κ-type.CONCLUSION: The soluble ScFv of MC3 is successfullyproduced, which not only provides a possible novel targetingvehicle for in vivo and in vitro study on associated cancers,but also offers the anuibody a stable genetic source. | Feng-Tian He Rong-Fen Li Yun-Sheng Kang Yan Zhang,Department of Biochemistry & Molecular Biology,Third Military Medical University,Chongqing 400038,China Yong-Zhan Nie Bao-Jun Chen Tai-Dong Qiao Dai-Ming Fan,Institute of Digestive Disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 13 |
| 12 | Angiostatin up-regulation in gastric cancer cell SGC7901 inhibits tumorigenesis in nude mice显示文摘AIM: To explore the influence of angiostatin up-regulationon the biologic behavior of gastric cancer cells in vitro andin vivo, and the potential of angiostatin gene therapy in thetreatment of human gastric cancer.METHODS: Mouse angiostatin cDNA was subcloned intothe eukaryotic expression vector pcDNA3.1(+) and identifiedby restriction endonucleases digestion and sequencing. Therecombinant vector pcDNA3. 1(+)-angio was transfected intohuman gastric cancer cells SGC7901 with liposome andparalleled with the vector control and the mock control.Angiostatin transcription and protein expression wereexamined by RT-PCR and Western blot in the stable celllines selected by G418. Cell proliferation and growth in vitroof the three groups were observed respectively undermicroscope, cell number counting and FACS. The cellsoverexpressing angiostatin, vector transfected and untreatedwere respectively implanted subcutaneously into nude mice.After 30days the size of tumors formed was measured, andmicrovessel density count (MVD) in the tumor tissues wasassessed by immunohistochemistry with the primary anti-vWF antibody.RESULTS: The recombinant vector pcDNA3.1(+)-angio wasconfirmed with the correct sequence of mouse angiostatinunder the promoter CMV. After 30 d of transfection andselection with G418, macroscopic resistant cell clones wereformed in the experimental group transfected with pcDNA3.1(+)-angio and the vector control. But no untreated cellssurvived in the mock control. Angiostatin mRNAtranscription and protein expression were detected in theexperimental group. No significant differences wereobserved among the three groups in cell morphology, cellgrowth curves and cell cycle phase distributions in vitro.However, in nude mice model, markedly inhibitedtumorigenesis and slowed tumor expansion were observedin the experimental group as compared with the controls,which was paralleled with decreased microvessel density inand around tumor tissues (P<0. 05).CONCLUSION: Angiostatin does not directly inhibit humangastric cancer cell proliferation and growth in vitro, but exertsits anti-tumor functions through antiangiogenesis in aparacrine way in vivo. | Jing Wu Yong-Quan Shi Kai-Chun Wu De-Xin Zhang Jing-Hua Yang Dai-Ming Fan Institute of Gastrointestinal Diseases Research,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shannxi Province,China | 2003 | World Journal of Gastroenterology2003，9，1： | 11 |
| 13 | Expression of PCNA and CD44mRNA in colorectal cancer with venous invasion and its relationship to liver metastasis显示文摘AIM: To investigate the expression of proliferating cell nuclear antigen (PCNA) and CD44mRNA in colorectal cancer with venous invasion and its relationship with liver metastasis.METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of PCNA and CD44mRNA in 31 cases of colorectal cancer with venous invasion.RESULTS: Positive expression rates of PCNA and CD44mRNA in colorectal cancer were higher than those without liver metastasis (P<0.05 and P<0.01). In case of colorectal cancer with liver metastasis, strongly positive rates of PCNA and CD44mRNA were 94.1% and 70.6 %,respectively, significantly higher than those without liver metastasis. There was a positive relationship between the expressions of PCNA and CD44mRNA (r=0.67, P<0.05).CONCLUSION: Detection of PCNA and CD44mRNA expression in colorectal cancer may be useful for evaluating liver metastasis of cancer cells. | Shu-Qiang Yue Yan-Ling Yang Ke-Feng Dou Kai-Zong Li Department of Hepatobiliary Surgery,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，12： | 11 |
| 14 | FOXP3 expression and clinical characteristics of hepatocellular carcinoma显示文摘AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessedto identify the clinical characteristics of FOXP3 in HCC. RESULTS: The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC. CONCLUSION: The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC. | Wei-Hua Wang, Jun-Zhi Wang, National Center for Safety Evaluation of Drugs, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China Wei-Hua Wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, Biotechnology Center, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Wei-Hua Wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, State Key Laboratory of Cancer Biology, Xi’an 710032, Shaanxi Province, China Wei Yan, Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Yu-Hai Zhang, Department of Health Statistics, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Jiang-Tao Yang, Department of Pathology, Xi’an Gaoxin Hospital, Xi’an 710032, Shaanxi Province, China | 2010 | World Journal of Gastroenterology2010，16，43： | 10 |
| 15 | Chondroitinase ABC plus bone marrow mesenchymal stem cells for repair of spinal cord injury显示文摘As chondroitinase ABC can improve the hostile microenvironment and cell transplantation is proven to be effective after spinal cord injury, we hypothesized that their combination would be a more effective treatment option. At 5 days after T8 spinal cord crush injury, rats were injected with bone marrow mesenchymal stem cell suspension or chondroitinase ABC 1 mm from the edge of spinal cord damage zone. Chondroitinase ABC was first injected, and bone marrow mesenchymal stem cell suspension was injected on the next day in the combination group. At 14 days, the mean Basso, Beattie and Bresnahan score of the rats in the combination group was higher than other groups. Hematoxylin-eosin staining showed that the necrotic area was significantly reduced in the combination group compared with other groups. Glial fibrillary acidic protein-chondroitin sulfate proteoglycan double staining showed that the damage zone of astrocytic scars was significantly reduced without the cavity in the combination group. Glial fibrillary acidic protein/growth associated protein-43 double immunostaining revealed that positive fibers traversed the damage zone in the combination group. These results suggest that the combination of chondroitinase ABC and bone marrow mesenchymal stem cell transplantation contributes to the repair of spinal cord injury. | Chun Zhang Xijing He Haopeng Li Guoyu Wang | 2013 | Neural Regeneration Research2013，8，11： | 10 |
| 16 | The relationship between concentration of growth hormone in serum and microangiopathy in patients with diabetes mellitus.显示文摘Therelationshipbetwenconcentrationofgrowthhormoneinserumandmicroangiopathyinpatientswithdiabetesmelitus.ChenMingsheng,YuWenbi... | Chen Mingsheng, Yu Wenbin, Hu Shaowen, et al. Clinical Laboratovy, Xijing Hospital, Xi’an 710032, China. | 1998 | Chinese Medical Journal1998，，1： | 9 |
| 17 | Preparation of single chain variable fragment of MG_7 mAb by phage display technology显示文摘AIM To develop the single chain variable fragment of MG7 murine anti-human gastric cancer monoclonal antibody using the phage display technology for obtaining a tumor-targeting mediator.METHODS mRNA was isolated from MG7-producing murine hybridoma cell line and converted into cDNA. The variable fragments of heavy and light chain were amplified separately and assembled into ScFv with a specially constructed DNA linker by PCR. The ScFvs DNA was ligated into the phagmid vector pCANTAB5E and the ligated sample was transformed into competent E. Coli TG1. The transformed cells were infected with M13K07 helper phage to form MG7 recombinant phage antibody library. The volume and recombinant rate of the library were evaluated by means of bacterial colony count and restriction analysis. After two rounds of panning with gastric cancer cell line KATOⅢ of highly expressing MG7binding antigen, the phage clones displaying ScFv of the antibody were selected by ELISA from the enriched phage clones. The antigen-binding affinity of the positive clone was detected by competition ELISA. HB2151 E. Coli was transfected with the positive phage clone demonstrated by competition ELISA for production of a soluble form of the MG7 ScFv. ELISA assay was used to detect the antigenbinding affinity of the soluble MG7 ScFv. Finally, the relative molecular mass of soluble MG7 ScFv was measured by SDS-PAGE.RESULTS The VH, VL and ScFv DNAs were about 340bp,320bp and 750bp, respectively. The volume of the library was up to 2 × 106 and 8 of 11 random clones were recombinants. Two phage clones could strongly compete with the original MG7 antibody for binding to the antigen expressed on KATO Ⅲ cells. Within 2 strong positive phage clones, the soluble MG7 ScFv from one clone was found to have the binding activity with KATO Ⅲ cells.SDS-PAGE showed that the relative molecular weight of soluble MG7 ScFv was 32.CONCLUSION The MG7 ScFv was successfully produced by phage antibody technology, which may be useful for broadening the scope of application of the antibody. | Zhao-Cai Yu Jie Ding Yong-Zhan Nie Dai-Ming Fan Xue-Yong Zhang Department of Gastroenterology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 9 |
| 18 | KAI1 inhibits HGF-induced invasion of pancreatic cancer by sphingosine kinase activity显示文摘BACKGROUND:KAI1/CD82 has been reported to attenuate the process of metastases in a variety of tumors;however,its mechanism of action in invasion has not been fully elucidated. The present study aimed to investigate the importance of KAI1 in invasion and its correlation with activation of sphingosine kinase(SPK)in human pancreatic cancer PANC1 and Miapaca-2 cell lines. METHODS:The expression of KAI1 in PANC1 and Miapaca-2 cells,which was mediated by recombinant adenovirus(Ad-KAI1), was assessed by a flow cytometer and Western blotting.After successful infection was established,in vitro growth curve and invasive ability in Boyden Chamber assay were studied.The presence of KAI1 correlating with c-Met and SPK was detected by co-immunoprecipitation and[γ-32P]ATP incorporation. RESULTS:KAI1 genes had no significant effects on the curve representing cell growth.After infection with the KAI1 gene,decreased invasive ability in the Boyden Chamber assay was observed in PANC1 and Miapaca-2 cells that were induced by hepatocyte growth factor.Over-expression of KAI1 in the cells led to the deactivation of SPK and a decreased level of intracellular sphingosine-1-phosphate.No correlation was observed between c-Met and KAI1 during co-immunoprecipitation. CONCLUSION:The results of this study for the first time demonstrated a regulatory role for KAI1 in SPK activation, which leads to decreased invasive ability in disease progression of human pancreatic cancer. | Xu Liu,Xiao-Zhong Guo,Wei-Wei Zhang,Zhuo-Zhuang Lu,Qun-Wei Zhang, Hai-Feng Duan and Li-Sheng Wang State Key Laboratory of Cancer Biology and Institute of Digestive Diseases,Xijing Hospital of Digestive Disease,Fourth Military Medical University,Xi’an 710032,China Department of Gastroenterology,Shenyang General Hospital of PLA,Shenyang 110016, China Department of Experimental Hematology,Beijing Institute of Radiation Medicine,Beijing 100850,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，2： | 9 |
| 19 | KAI1/CD82 suppresses hepatocyte growth factorinduced migration of hepatoma cells via upregulationof Sprouty2显示文摘We conducted a study concerning the suppressive mechanism of KAI1/CD82 on hepatoma cell metastasis.Hepatocyte growth factor(HGF)induces the migration of hepatoma cells through activation of cellular sphingosine kinase 1(SphK1).Adenovirus-mediated gene transfer of KAI1(Ad-KAI1)downregulates the SphK1 expression and suppresses the HGF-induced migration of SMMC-7721 human hepatocellcular carcinoma cells.Overexpression of KAI1/CD82 significantly elevates Sprouty2 at the protein level.Ablation of Sprouty2 with RNA interference can block the KAI1/CD82-induced suppression of hepatoma cell migration and downregulation of SphK1 expression.It is demonstrated that KAI1/CD82 suppresses HGF-induced migration of hepatoma cells via upregulation of Sprouty2. | MU ZhenBin1 ,3,WANG Hua 2,ZHANG Jing 2,LI QingFang 2,WANG LiSheng 2&GUO XiaoZhong 3 1State Key Laboratory of Cancer Biology and Institute of Digestive Diseases,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China 2Department of Experimental Hematology,Beijing Institute of Radiation Medicine,Beijing 100850,China 3Department of Gastroenterology,Shenyang General Hospital of PLA,Shenyang 110016,China | 2008 | Science China(Life Sciences)2008，51，7： | 7 |
| 20 | Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells显示文摘AIM: To investigate the expression level of ZNRD1 gene in gastric cancer cells SGC7901 and gastric cancer MDR (multidrug resistant) cells SGC7901/VCR, and to observe the drug sensitizing and proliferation effect of ZNRD1 antisense nucleic acid transduction on SGC7901/VCR cells.METHODS: Amplification of sequences encoding ZNRD1 from SGC7901/VCR cDNA by PCR. The levels of ZNRD1 mRNA expression were demonstrated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR).Eukaryotic expression vector pcDNA3.1-anti ZNRD1 was constructed and transfected into SGC7901/VCR cells by lipofectamine. Immunochemical method was used to detect the expression of protein in SGC7901/VCR cells and transfectants. The cell cycle alteration and the intracellular adriamycin (ADM) accumulation were observed by FACS.Growth curve and drug sensitization of cells for vincristine (VCR) were analyzed with MTT assay.RESULTS: We cloned the open reading frame of full-length ZNRD1. The expression of ZNRD1 showed higher in SGC7901/VCR than in SGC7901 cells. The antisense ZNRD1 drug-resistant clones were selected after gene transfection.Immunochemical results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than that in non-transfectants. Comparing to SGC7901/VCR and pcDNA3.1-SGC7901/VCR, anti ZNRD1-SGC7901/VCR showed gradually accumulated in G1 phase, with aconcomitant decrease of cell population in S phase. FACSalso suggested intracellular ADM accumulation increased2fold in SGC7901/VCR cells after transfected with antisenseZNRD1.MTT assay showed that transfectant cells proliferationwas lagged and more sensitive to vcr than non-transfectants.CONCLUSION: ZNRD1 gene displayed highly expression in VCR resistant gastric cancer cells.Expression of ZNRD1 protein was effectively blockde in anti ZzNRD1-SGC7901/VCR cells by gene transfection.ZNRD1 antisense nucleic acid transfection sensitized drug resistant gastric cancer cells to VCR,increased ADM accumulation and inhibited the cells to VCR,incereased ADM accumulation qand inhibited the cells proliferation.ZNRS1 antisense RNA transduction could reverse the MDR of human drug-resistant gastric cancer cell SGC7901/VCR to a degree. | Yu-Mei Zhang Yan-Qiu Zhao Yang-Lin Pan Yong-Quan Shi Xiao-Hang Jin Hui Yi Dai-Ming Fan Department of Gastroenterology,Xijing Hospital,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 7 |
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