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| 1 | Exploration of the formation mechanism and source attribution of ambient ozone in Chongqing with an observation-based model显示文摘An intensive field campaign was conducted in Chongqing during the summer of 2015 to explore the formation mechanisms of ozone pollution. The sources of ozone, the local production rates, and the controlling factors, as well as key species of volatile organic compounds(VOCs), were quantified by integrating a local ozone budget analysis, calculations of the relative incremental reactivity, and an empirical kinetic model approach. It was found that the potential for rapid local ozone formation exists in Chongqing. During ozone pollution episodes, the ozone production rates were found to be high at the upwind station Nan Quan, the urban station Chao Zhan, and the downwind station Jin-Yun Shan. The average local ozone production rate was 30×10^(-9) V/V h^(-1) and the daily integration of the produced ozone was greater than 180×10^(-9) V/V. High ozone concentrations were associated with urban and downwind air masses. At most sites, the local ozone production was VOC-limited and the key species were aromatics and alkene, which originated mainly from vehicles and solvent usage. In addition, the air masses at the northwestern rural sites were NO_x-limited and the local ozone production rates were significantly higher during the pollution episodes due to the increased NOx concentrations. In summary, the ozone abatement strategies of Chongqing should be focused on the mitigation of VOCs. Nevertheless, a reduction in NO_x is also beneficial for reducing the regional ozone peak values in Chongqing and the surrounding areas. | SU Rong LU KeDing YU JiaYan TAN ZhaoFeng JIANG MeiQing LI Jing XIE ShaoDong WU YuSheng ZENG LiMin ZHAI ChongZhi ZHANG YuanHang | 2018 | Science China Earth Sciences2018，61，1： | 24 |
| 2 | Adenovirus-mediated gene delivery:Potential applications for gene and cell-based therapies in the new era of personalized medicine显示文摘With rapid advances in understanding molecular pathogenesis of human diseases in the era of genome sciences and systems biology,it is anticipated that increasing numbers of therapeutic genes or targets will become available for targeted therapies.Despite numerous setbacks,efficacious gene and/or cell-based therapies still hold the great promise to revolutionize the clinical management of human diseases.It is wildly recognized that poor gene delivery is the limiting factor for most in vivo gene therapies.There has been a long-lasting interest in using viral vectors,especially adenoviral vectors,to deliver therapeutic genes for the past two decades.Among all currently available viral vectors,adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types.The applications of adenoviral vectors in gene delivery have greatly increased in number and efficiency since their initial development.In fact,among over 2000 gene therapy clinical trials approved worldwide since 1989,a significant portion of the trials have utilized adenoviral vectors.This review aims to provide a comprehensive overview on the characteristics of adenoviral vectors,including adenoviral biology,approaches to engineering adenoviral vectors,and their applications in clinical and preclinical studies with an emphasis in the areas of cancer treatment,vaccination and regenerative medicine.Current challenges and future directions regarding the use of adenoviral vectors are also discussed.It is expected that the continued improvements in adenoviral vectors should provide great opportunities for cell and gene therapies to live up to its enormous potential in personalized medicine. | Cody S.Lee Elliot S.Bishop Ruyi Zhang Xinyi Yu Evan M.Farina Shujuan Yan Chen Zhao Zongyue Zeng Yi Shu Xingye Wu Jiayan Lei Yasha Li Wenwen Zhang Chao Yang Ke Wu Ying Wu Sherwin Ho Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Russell R.Reid Tong-Chuan He | 2017 | Genes & Diseases2017，4，2： | 19 |
| 3 | Study on the antiulcer effects of Veronicastrum axillare on gastric ulcer in rats induced by ethanol based on tumor necrosis factor-α(TNF-α)and endothelin-1(ET-1)显示文摘Objective:To assess whether Veronicastrum axillare(V.axillare)can ameliorate ethanol-induced gastric mucosal lesions in rats,reduce the production of pro-inflammatory cytokines,suppress apoptosis and improve local microcirculation disturbances.Methods:Totally 48 male Sprague-Dawley rats were randomly divided into six groups,eight rats in each group.Rats in the normal group and the model group were administered with 0.9%normal saline respectively.Rats in the positive group and ranitidine group were administered with 0.18%ranitidine suspension by intragastric administration respectively.Those in the high dose V.axillare group,the medium dose V.axillare group and the low dose V.axillare group were administrated with V.axillare at the daily dose of 2.8 g/kg,1.4 g/kg and 0.7 g/kg by intragastric administration.Gastric mucosal lesions were produced by intragastric administration of absolute ethanol.Water extract of V.axillare was successively injected for 14 d and last day was injected 1 h before ethanol administration.Gastric mucosal ulcer index and ulcer inhibitory rate were counted by improved Guth methods.The tissue sections were made for pathological histology analysis.Also,we measured the concentrations of tumor necrosis factor-α(TNF-α)and endothelin-1(ET-1)in gastric mucosal,as an index of the pro-inflammatory cytokines,apoptosis and local microcirculation.Besides,the mRNA contents of TNF-αand ET-1 were measured to verify effects on gene expression by real-time fluorescent quantitative PCR.Results:Water extract of V.axillare significantly ameliorated the gastric mucosal lesions induced by ethanol administration(P<0.01).Pro-inflammatory cytokines,TNF-a and ET-1 were increased after ethanol administration and significantly reduced by water extract of V.axillare.The expressions of TNF-αand ET-1 mRNA were also be inhibited by water extract of V.axillare.Conclusion:Current evidences show water extract of V.axillare is effective for defending against ethanol-induced gastric mucosal lesions,significantly inhibiting the production of proinflammatory cytokines and the expressions of TNF-αand ET-1 mRNA,which may be useful for inhibiting apoptosis and improving local microcirculation. | Yong Du Weichun Zhao Leilei Lu Jiayan Zheng Xishi Hu Zhehan Yu Lixin Zhu | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，12： | 13 |
| 4 | Phosphorylation of LTF1, an MYB Transcription Factor in Populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells显示文摘Lignin is specifically deposited in plant secondary cell walls,and initiation of lignin biosynthesis is regulated by a variety of developmental and environmental signals.However,the mechanisms governing the regulation of lignin biosynthesis remain to be elucidated.In this study,we identified a lignin biosynthesis-associated transcription factor(LTF)from Populus,LTF1,which binds the promoter of a key lignin biosynthetic gene encoding 4-coumarate-CoA ligase(4CL).We showed that LTF1 in its unphosphorylated state functions as a regulator restraining lignin biosynthesis.When LTF1 becomes phosphorylated by PdMPK6 in response to external stimuli such as wounding,it undergoes degradation through a proteasome pathway,resulting in activation of lignification.Expression of a phosphorylation-null mutant version of LTF1 led to stable protein accumulation and persistent attenuation of lignification in wood cells.Taken together,our study reveals a mechanism whereby LTF1 phosphorylation acts as a sensory switch to regulate lignin biosynthesis in response to environmental stimuli.The discovery of novel modulators and mechanisms modifying lignin biosynthesis has important implications for improving the utilization of cell-wall biomass. | Jinshan Gui Laifu Luo Yu Zhong Jiayan Sun Toshiaki Umezawa Laigeng Li | 2019 | Molecular Plant2019，12，10： | 10 |
| 5 | Characterization of atomic defects on the photoluminescence in two-dimensional materials using transmission electron microscope显示文摘Two-dimensional material(2D)that possesses atomic thin geometry and remarkable properties is a star material for the fundamental researches and advanced applications.Defects in 2D materials are critical and fundamental to understand the chemical,physical,and optical properties.Photoluminescence arises in 2D materials owing to various physical phenomena including activator/dopant-induced luminescence and defect-related emissions,and so forth.With the advanced transmission electron microscopy(TEM)technologies,such as aberration correction and low voltage technologies,the morphology,chemical compositions and electronic structures of defects in 2D material could be directly characterized at the atomic scale.In this review,we introduce the applications of state-of-the-art TEM technologies on the studies of the role of atomic defects in the photoluminescence characteristics in 2D material.The challenges in spatial and time resolution are also discussed.It is proved that TEM is a powerful tool to pinpoint the relationship between the defects and the photoluminescence characteristics. | Jiayan Zhang Ye Yu Peng Wang Chen Luo Xing Wu Ziqi Sun Jianlu Wang Wei Da Hu Guozhen Shen | 2019 | InfoMat2019，1，1： | 6 |
| 6 | Dendritic platinum-copper bimetallic nanoassemblies with tunable composition and structure: Arginine-driven self-assembly and enhanced electrocatalytic activity显示文摘 | Gengtao Fu Huimin Liu Nika You Jiayan Wu Dongmei Sun Lin Xu Yawen Tang Yu Chen | 2016 | Nano Research2016，9，3： | 6 |
| 7 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 8 | Tolerance of Physocypria kraepelini (Crustacean, Ostracoda) to water-borne ammonia, phosphate and pH value显示文摘This study evaluated the median lethal concentration (LC50) and safe concentration of water-borne ammonia, phosphate and pH value on Physocypria kraepelini, a freshwater Ostracoda with a static renewal test system. The results indicated that the LC50 values of ammonia for P. kraepelini were 1026.71, 859.98, 771.79 and 583.82 mg/L at 24, 48, 72 and 96 h exposure, respectively, and the safe concentration range of ammonia for the long-term survival of P. kraepelini was less than 58.38 mg/L. The safe range of pH value for the survival of P. kraepelini was from 6.59 to 7.61. P. kraepelini has a high tolerance to ammonia, phosphate and pH value which are the main environmental factors in the serious eutrophication water. | YU Na, CHEN Shimei, LI Erchao, CHEN Jiayan, CHEN Liqiao School of Life Science, East China Normal University, Shanghai 200062, China | 2009 | Journal of Environmental Sciences2009，21，11： | 4 |
| 9 | Arginine-mediated synthesis of cube-like platinum nanoassemblies as efficient electrocatalysts显示文摘可控制高贵金属 nanocrystals 自己组装具有为高度活跃的 electrocatalysts 的发展的宽广兴趣。这里,我们与多孔的洞和不平的表面为像立方体的磅 nanoassemblies (Pt-CNAs ) 的产量很高的合成报导一条有效调停精氨酸的热水的途径基于零维的磅 nanocrystals 自己组装。在这进程,精氨酸充当在邻近的 nanocrystals 之间的 reductant,结构指导代理人,和连接器。有趣地, Pt-CNAs 展览单个水晶的结构与主导 { 100 } 方面由 X 光检查衍射证实了。把研究基于 electrocatalytic,同样综合的 Pt-CNAs 展览在甲醇氧化反应改进了 electrocatalytic 活动以及好稳定性和公司忍耐。Pt-CNAs 好表演被归因于他们的唯一的形态学和表面结构。我们相信这里构画出的合成策略能被递讲道理地设计的其它为在高效燃料房间的使用的一金属或二金属的 nanoassemblies。 | Gengtao Fu Qian Zhang Jiayan Wu Dongmei Sun Lin Xu Yawen Tang Yu Chen | 2015 | Nano Research2015，8，12： | 4 |
| 10 | DNA sequencing leads to genomics progress in China显示文摘1 Science in the large-scale sequencing era Ten years ago,the first draft sequence assembly of the human genome was completed [1],bringing biomedical research one-step closer toward the goal of revolutionizing diagnosis,prevention,and treatment of human diseases.Recently,journalists from the journal Nature surveyed more than 1000 life scientists regarding this laudable aim [2],obtaining substantially negative responses [3].However,almost all of those surveyed had been influenced,in one way or another,by the availability of the human genome sequence,and they also agreed with the notion that the 'sequence is the start.' The complexity of genome biology and almost every aspect of human biology is far greater than previously thought [4]. | WU JiaYan XIAO JingFa ZHANG RuoSi YU Jun | 2011 | Science China(Life Sciences)2011，54，3： | 4 |
| 11 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 12 | Transcriptomic analysis reveals key regulators of mammogenesis and the pregnancy-lactation cycle显示文摘An organ unique to mammals,the mammary gland develops 90%of its mass after birth and experiences the pregnancy-lactation-involution cycle(PL cycle)during reproduction.To understand mammogenesis at the transcriptomic level and using a ribo-minus RNA-seq protocol,we acquired greater than 50 million reads each for the mouse mammary gland during pregnancy(day 12 of pregnancy),lactation(day 14 of lactation),and involution(day 7 of involution).The pregnancy-,lactation-and involution-related sequencing reads were assembled into 17344,10160,and 13739 protein-coding transcripts and1803,828,and 1288 non-coding RNAs(ncRNAs),respectively.Differentially expressed genes(DEGs)were defined in the three samples,which comprised 4843 DEGs(749 up-regulated and 4094 down-regulated)from pregnancy to lactation and4926 DEGs(4706 up-regulated and 220 down-regulated)from lactation to involution.Besides the obvious and substantive upand down-regulation of the DEGs,we observe that lysosomal enzymes were highly expressed and that their expression coincided with milk secretion.Further analysis of transcription factors such as Trps1,Gtf2i,Tcf7l2,Nupr1,Vdr,Rb1,and Aebp1,and ncRNAs such as mir-125b,Let7,mir-146a,and mir-15 has enabled us to identify key regulators in mammary gland development and the PL cycle. | ZHOU YuanYuan GONG Wei XIAO JingFa WU JiaYan PAN LinLin LI XiaoNuan WANG XuMin WANG WeiWei HU SongNian YU Jun | 2014 | Science China(Life Sciences)2014，57，3： | 4 |
| 13 | Systematic analysis of intron size and abundance parameters in diverse lineages显示文摘All eukaryotic genomes have genes with introns in variable sizes.As far as spliceosomal introns are concerned,there are at least three basic parameters to stratify introns across diverse eukaryotic taxa:size,number,and sequence context.The number parameter is highly variable in lower eukaryotes,especially among protozoan and fungal species,which ranges from less than4%to 78%of the genes.Over greater evolutionary time scales,the number parameter undoubtedly increases as observed in higher plants and higher vertebrates,reaching greater than 12.5 exons per gene in average among mammalian genomes.The size parameter is more complex,where multiple modes appear at work.Aside from intronless genes,there are three other types of intron-containing genes:half-sized,minimal,and size-expandable introns.The half-sized introns have only been found in a limited number of genomes among protozoan and fungal lineages and the other two types are prevalent in all animal and plant genomes.Among the size-expandable introns,the sizes of plant introns are expansion-limited in that the large introns exceeding 1000 bp are fewer in numbers and transposon-free as compared to the large introns among animals,where the larger introns are filled with transposable elements and appear expansion-flexible,reaching several kilobasepairs(kbp)and even thousands of kbp in size.Most of the intron parameters can be studied as signatures of the specific splicing machineries of different eukaryotic lineages and are highly relevant to the regulation of gene expression and functionality.In particular,the transcription-splicing-export coupling of eukaryotic intron dispensing leads to a working hypothesis that all intron parameters are evolved to be efficient and function-related in processing and routing the spliced transcripts. | WU JiaYan XIAO JingFa WANG LingPing ZHONG Jun YIN HongYan WU ShuangXiu ZHANG Zhang YU Jun | 2013 | Science China(Life Sciences)2013，56，10： | 4 |
| 14 | PtrCel9A6, an Endo-l,4-β-Glucanase, Is Required for Cell Wall Formation during Xylem Differentiation in Populus显示文摘 | Liangliang Yu Jiayan Sun Laigeng Li | 2013 | Molecular Plant2013，6，6： | 3 |
| 15 | Coevolution study of mitochondria respiratory chain proteins:Toward the understanding of protein-protein interaction显示文摘Coevolution can be seen as the interdependency between evolutionary histories.In the context of protein evolution,functional correlation proteins are ever-present coordinated evolutionary characters without disruption of organismal integrity.As to complex system,there are two forms of protein—protein interactions in vivo,which refer to inter-complex interaction and intra-complex interaction.In this paper,we studied the difference of coevolution characters between inter-complex interaction and intra-complex interaction using 'Mirror tree' method on the respiratory chain(RC) proteins.We divided the correlation coefficients of every pairwise RC proteins into two groups corresponding to the binary protein—protein interaction in intra-complex and the binary protein—protein interaction in inter-complex,respectively.A dramatical discrepancy is detected between the coevolution characters of the two sets of protein interactions(Wilcoxon test,p-value = 4.4×10^(-6)).Our finding reveals some critical information on coevolutionary study and assists the mechanical investigation of protein—protein interaction. Furthermore,the results also provide some unique clue for supramolecular organization of protein complexes in the mitochondrial inner membrane.More detailed binding sites map and genome information of nuclear encoded RC proteins will be extraordinary valuable for the further mitochondria dynamics study. | Ming Yang Yan Ge Jiayan Wu Jingfa Xiao Jun Yu | 2011 | Journal of Genetics and Genomics2011，38，5： | 3 |
| 16 | Metabolic risk factors associated with sudden cardiac death(SCD)during acute myocardial ischemia显示文摘Sudden cardiac death(SCD)is the leading cause of death worldwide.Myocardial ischemia(MI)is the most common underlying causal disorder for SCD.Metabolic risks leading to SCD during acute MI are still not fully understood.Here,using tissue metabolomics,we aimed to investigate myocardial metabolic alterations relevant to SCD events in an acute MI rat model induced by coronary artery ligation(CAL).Thirty-four rats were successfully performed CAL,of which 13 developed lethal ventricular tachyarrhythmia(LVTA)-SCD and 7 developed severe atrioventricular block(AB)-SCD.Fourteen rats that survived within 70 min after the ligation were served as peer controls.The partial least squares-discriminant analysis plots demonstrated clear separations between the SCD rats and controls,indicating obvious differences in myocardial metabolome between these rats.The levels of isoleucine,lactate,glutamate choline,phosphorylcholine,taurine and asparagine in ischemic myocardia were positively associated with LVTA-SCD events;in contrast,the levels of alanine,urea,phenylalanine,linoleic acid,elaidic acid and stearic acid were inversely correlated with LVTA-SCD events.The levels of glutamate and urea were positively and negatively relevant to AB-SCD events,respectively.The dangerous metabolites indicated that lower levels of energy substrates,severe hypoxia,the inhibition of transamination and hyper sympathetic excitement and reactive oxygen species in myocardia were vulnerable to SCD during acute MI.The results suggest fatal metabolic alterations correlated with SCD events during acute MI,which could offer novel clues for the prevention or treatment of acute MI-related SCD. | Dian Wang Xingxing Wang Jiayan Wu Ruibing Su Jing Kong Xiaojun Yu | 2017 | Forensic Sciences Research2017，2，3： | 2 |
| 17 | Ribogenomics: the Science and Knowledge of RNA显示文摘Ribonucleic acid(RNA) deserves not only a dedicated field of biological research –– a discipline or branch of knowledge –– but also explicit definitions of its roles in cellular processes and molecular mechanisms. Ribogenomics is to study the biology of cellular RNAs, including their origin, biogenesis, structure and function. On the informational track, messenger RNAs(mRNAs) are the major component of ribogenomes, which encode proteins and serve as one of the four major components of the translation machinery and whose expression is regulated at multiple levels by other operational RNAs. On the operational track, there are several diverse types of RNAs –– their length distribution is perhaps the most simplistic stratification –– involving in major cellular activities, such as chromosomal structure and organization, DNA replication and repair, transcriptional/ post-transcriptional regulation, RNA processing and routing, translation and cellular energy/ metabolism regulation. An all-out effort exceeding the magnitude of the Human Genome Project is of essence to construct just mammalian transcriptomes in multiple contexts including embryonic development, circadian and seasonal rhythms, defined life-span stages, pathological conditions and anatomy-driven tissue/organ/cell types. | Jiayan Wu Jingfa Xiao Zhang Zhang Xumin Wang Songnian Hu Jun Yu | 2014 | Genomics, Proteomics & Bioinformatics2014，12，2： | 2 |
| 18 | Photothermal treatment with EGFRmAb-AuNPs induces apoptosis in hypopharyngeal carcinoma cells via PI3K/AKT/mTOR and DNA damage response pathways显示文摘 | Yingying Zhang Linhai Cong Jiayan He Yu Wang Yingying Zou Zhihong Yang Yuexin Hu Shiwen Zhang Xiaoguang He | 2018 | Acta Biochimica et Biophysica Sinica2018，50，6： | 2 |
| 19 | Supply-Demand Analysis of Urban Emergency Shelters Based on Spatiotemporal Population Estimation显示文摘Supply–demand analysis is an important part of the planning of urban emergency shelters.Using Pudong New Area,Shanghai,China as an example,this study estimated daytime and nighttime population of the study area based on fine-scale land use data,census data,statistical yearbook information,and Tencent user-density big data.An exponential function-based,probability density estimation method was used to analyze the spatial supply of and demand for shelters under an earthquake scenario.The results show that even if all potential available shelters are considered,they still cannot satisfy the demand of the existing population for evacuation and sheltering,especially in the northern region of Pudong,under both the daytime and the nighttime scenarios.The proposed method can reveal the spatiotemporal imbalance between shelter supply and demand.We also conducted a preliminary location selection analysis of shelters based on the supply–demand analysis results.The location selection results demonstrate the advantage of the proposed method.It can be applied to identify the areas where the supply of shelters is seriously inadequate,and provide effective decision support for the planning of urban emergency shelters. | Xiaodong Zhang Jia Yu Yun Chen Jiahong Wen Jiayan Chen Zhan'e Yin | 2020 | International Journal of Disaster Risk Science2020，11，4： | 2 |
| 20 | A Brief Review of Software Tools for Pangenomics显示文摘Since the proposal for pangenomic study, there have been a dozen software tools actively in use for pangenomic analysis. By the end of 2014, Panseq and the pan-genomes analysis pipeline(PGAP) ranked as the top two most popular packages according to cumulative citations of peerreviewed scientific publications. The functions of the software packages and tools, albeit variable among them, include categorizing orthologous genes, calculating pangenomic profiles, integrating gene annotations, and constructing phylogenies. As epigenomic elements are being gradually revealed in prokaryotes, it is expected that pangenomic databases and toolkits have to be extended to handle information of detailed functional annotations for genes and non-protein-coding sequences including non-coding RNAs, insertion elements, and conserved structural elements. To develop better bioinformatic tools, user feedback and integration of novel features are both of essence. | Jingfa Xiao Zhewen Zhang Jiayan Wu Jun Yu | 2015 | Genomics, Proteomics & Bioinformatics2015，13，1： | 1 |
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