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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | 星形胶质细胞转录组分析指导的脑卒中靶标鉴定显示文摘星形胶质细胞支持正常的脑功能,但在卒中等病理条件下,星形胶质细胞反应性激活,其也可能参与神经退行性改变。反应性星形胶质细胞增生对机体有益或有害,与环境背景有关,但其分子基础仍未得到充分解释。借助RiboTag技术,我们在特异性地短暂性大脑中动脉闭塞(tMCAO)72h后的小鼠星形胶质细胞中,纯化翻译mRNA,构建卒中特异性星形胶质细胞翻译组数据库。我们发现,与对照组脑组织相比,tMCAO后的反应性星形胶质细胞显示与A2表型相关的转录物的富集,这与神经保护相关。星形胶质细胞也会上调大量潜在的神经毒性基因。我们共鉴定了1003个基因和38个转录因子的差异表达,其中Stat3,Sp1和Spi1变化最明显。为了进一步探索Stat3介导的通路对卒中发病机制的影响,我们选择星形胶质细胞特异性条件性缺失Stat3的小鼠制造tMCAO模型,发现这些小鼠的局灶性缺血卒中体积减少,且梗死72h后小鼠的运动结果改善。综上所述,本研究扩展了新兴的星形胶质细胞特异性靶向卒中治疗数据库,并肯定了星形胶质细胞是脑功能的关键保障细胞。 | Rakers C Schleif M Blank N Matu?ková H Ulas T H?ndler K Torres SV Schumacher T Tai K Schultze JL Jackson WS Petzold GC 聂昊 | 2019 | 神经损伤与功能重建2019，14，6： | 2 |
| 3 | Cloning the swamp buffalo SRY gene for embryo sexing with multiplex-nested PCR 显示文摘 | Fu Q Zhang M Qin WS | 2007 | Theriogenology2007，68，： | 1 |
| 4 | Hypereosinophilic syndrome:a case series显示文摘 | Aziz M Khan WS Ammar T el al | 2009 | Biomedica2009，25，3： | 1 |
| 5 | Atriopeptin lowers aqueous humor formation and intraccular pressure and elevates ciliary cyclic GMP but lacks uveal vascular effects in the bovine perfused eye显示文摘 | MILLAR JC SHAHIDULLAH M WILSON WS | 1997 | J Ocul Pharmacol Ther1997，13，1： | 1 |
| 6 | Inflammatory mediators induced by intratracheal instillation of ultrafine amorphous silica particles 显示文摘 | Cho WS Choi M Han BS | 2007 | Toxicol Lett2007，175，123： | 1 |
| 7 | Ten most important things to learnfrom the ACCF/AHA 2011expert consenus documenton hypertension in the elderly显示文摘 | Aronow WS Banch M | 2012 | Blood pressure2012，21，1： | 1 |
| 8 | Usefulness of echocardiographic left ventricular hypertrophy, ventricular tachy-cardia and complex ventricular arrhythmias in predicting ventricu-lar fibrillation or sudden cardiac death in elderly patients显示文摘 | Aronow WS Epstein S Koenigsberg M | 1988 | Am J Cardiol1988，62，16： | 1 |
| 9 | The multifaceted role of Notch in cancer显示文摘 | Roy M Pear WS Aster JC | 2007 | Curr Opin Genet Dev2007，17，1： | 1 |
| 10 | Atriopeptin, sodium azide arid cyclic GMP reduce secretion of aqueous humour and inhibit intracellular calcium release in bovine cultured ciliary epithelium 显示文摘 | SHAHIDULLAH M WILSON WS | 1999 | Br J Pharmacol1999，127，6： | 1 |
| 11 | Carvedilol inhibits clinical progress in patients with mild symptoms of heart failure显示文摘 | Colucci WS Packer M | 1996 | Cimulation1996，94，： | 1 |
| 12 | A male-specific expression gene,encodes a novel anionic antimicrobial peptide,acygonadin,in Scylla serrata显示文摘 | WANG KJ HUANG WS YANG M | 2007 | Mol Immunol2007，44，8： | 1 |
| 13 | Modulation of the immunogenicity of virus-like particles composed of mutant hepatitis B virus envelope subunits显示文摘 | Cheong WS Hyakumura M Yuen L | 2012 | Antiviral Res2012，93，2： | 1 |
| 14 | Electroactive monolayer substrates that selectively release adherent cells 显示文摘 | Yeo WS Hodneland CD Mrksieh M | 2001 | Chembio- chem2001，2，78： | 1 |
| 15 | 1-Hz low frequency re- petitive transcranial magnetic stimulation in children with Tourene's syndrome显示文摘 | Kwon HJ Lim WS Lim MH et M | 2011 | Neurosci Lett2011，492，1： | 1 |
| 16 | Platelet-rich plasma:a review of biology and aplications in plastic surgery显示文摘 | Eppley BL Pietrzak WS Blanton M | | 0，，06： | 1 |
| 17 | Dynamic perfusion CT: optimizing the temporal resolution and contrast volume for calculation of perfusion CT parameters in stroke patients 显示文摘 | Wintennark M Smith WS Ko Nu | 2004 | American Journal of Neumradiology2004，25，： | 1 |
| 18 | Atypical chryseobacte rium meningosepticumand meningitis and sepsis in newborns and the immunocompromised, Taiwan显示文摘 | Chiu CH Waddingdon M Hsieh WS | 2000 | Emerg Infect Dis2000，6，5： | 1 |
| 19 | Carbon dioxide in the prevention of air embolism during open-heart surgery显示文摘 | Rosen M | 1968 | Thorax1968，23，2： | 1 |
| 20 | n-3 Fatty acids from fish or fish-oil supplements,but not alphalinolenic acid,benefit cardiovascular disease outcomes in primary-and secondary-prevention studies:a systematic review显示文摘 | Wang C Harris WS Chung M | 2006 | Am J Clin Nutr2006，84，1： | 1 |
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