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| 1 | Intestinal endotoxemia as a pathogenetic mechanism in liver failure显示文摘Liver injury induced by various pathogenic factors (such as hepatitis virus, ethanol, drugs and hepatotoxicants, etc.)through their respective special pathogenesis is referred to as 'primary liver injury' (PLI). Liver injury resultedfrom endotoxin (lipopolysaccharide, LPS) and the activation of Kupffer cells by LPS while intestinal endotoxemia (IETM) occurred during the occurrence and development of hepatitis is named the 'secondary liver injury' (SLI).The latter which has lost their own specificities of primary pathogenic factors is ascribed to IETM. The 'secondary liver injury' is of important action and impact on development and prognosis of hepatitis. More severe IETM commonly results in excessive inflammatory responses, with serious hepatic necrosis,further severe hepatitis and even induces acute liver failure.The milder IETM successively precipitates a cascade,including repeated and persistent hepatocytic impairment accompanied by infiltration of inflammatory cells, hepatic fibrosis, cirrhosis and hepatocarcinoma. Generally, the milder IETM ends with chronic hepatic failure. If PLI caused by various pathogenic factors through their independent specific mechanismis regarded as 'the first hit' on liver, then SLI mediated by different chemical mediators from KCs activated by IETM in the course of hepatitis is 'the second hit' on liver. Thus, fusing and overlapping of the primary and scondary liver injuries determine and influeuce the complexity of the illness and outcome of the patient with hepatitis. For this reason, the viewpoint of 'SLI' induced by the 'second hit' on liver inflicted by IFTM suggests that medical professionals should attach great importance to both 'PLI'and 'SLI' caused by IETM. That is, try to adjust the function of KSs and eliminate endotoxemia of the patient. | De-Wu Han,Institute of Hepatology,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，6： | 170 |
| 2 | Methodologic research on TIMP-1,TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance显示文摘AIM: To set up a new method to detect tissue inhibitors ofmetalloproteinase1 and -2(TIMP-1 and TIMP-2) in sero ofpatients with hepatic cirrhosis, and to investigate theexpression and location of TIMP-1 and TIMP-2 in liver tissueof patients with hepatic cirrhosis, and the correlationbetween TIMPs in liver and those in sera so as to discusswhether TIMPs can be used ss a diagnosis index of hepaticfibrosisMETHODS: The monoclonal antibodies (McAbs) of TIMP-1and TIMP-2 were used to sensitize erythrocytes, and solid-phase absorption to sensitized erythrocytes (SPASE) wasused to detect TIMP-1 and TIMP-2 in the sera of patients withhepatic cirrhosis. Meanwhile, with the method of in situhybridization and immunohistochemistry, we studied themRNA expression and antigen location of TIMP-1 and TIMP-2in the livers of 40 hepatic cirrhosis patients with pathologicdiagnosis.RESULTS: With SPASE, they were 16.4 % higher in theacute hepatitis group, 33.3 % higher in the chronic hepatitisgroup, and the positive rates were 73.6 % and 61. 2 %respectively in sero of hepatic cirrhosis patients, which wereremarkably higher than those in chronic hepatitis and acutehepatitis group ( P < 0. 001 ). In 40 samples of hepaticcirrhosis tissues, all of them showed positive expression ofTIMP-1 and TIMP-2 mRNA detected withimmunohistochemistry or in situ hybridization (positive ratewas 100 % ). Expression of TIMPs in different degrees couldbe found in liver tissue with cirrhosis. TIMPs were located incytoplasm of liver cells of patients with hepatic cirrhosis.There was a significant correlation between serum TIMPslevel and liver TIMPs level.CONCLUSION: SPASE is a useful method to detect the TIMP-1 and TIMP-2 in sera of patients with hepatic cirrhosis, andTIMP-1 and TIMP-2 can be considered as a useful diagnosticindex of hepatic fibrosis, especially TIMP-1. | Oing-He Nie Yong-Oian Cheng Yu-Mei Xie Yong-Xing Zhou Bai-Xian Guang Yi-Zhan Cao,The Centre of Diagnosis and Treatment for Infectious Disease of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 51 |
| 3 | Information granules and entropy theory in information systems显示文摘Information granulation and entropy theory are two main approaches to research uncertainty of an information system,which have been widely applied in many practical issues. In this paper,the characterizations and representations of infor-mation granules under various binary relations are investigated in information systems,an axiom definition of information granulation is presented,and some existing definitions of information granulation become its special forms. Entropy theory in information systems is further developed and the granulation monoton-icity of each of them is proved. Moreover,the complement relationship between information granulation and entropy is established. This investigation unifies the results of measures for uncertainties in complete information systems and incom-plete information systems. | LIANG JiYe & QIAN YuHua Key Laboratory of Computational Intelligence and Chinese Information Processing,Ministry of Education School of Computer & Information Technology,Shanxi University,Taiyuan 030006,China | 2008 | Science in China(Series F)2008，51，10： | 41 |
| 4 | 新时代护士处方权内容专家共识显示文摘目前,国外已有22个国家的护士有了一定范围的处方权,并且有处方权的国家也在逐年增加,国外护士处方权的发展经历了漫长曲折的过程,现已被人们接受。2017年国际护士大会上英国学者介绍了有关护士处方权的现状和历史变革[1-2];美国医疗安全管理专家介绍了美国护士处方权的发展进程[3]。 | The First Hospital of Shanxi Medical University | 2018 | 护理研究2018，32，1： | 43 |
| 5 | Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles显示文摘尽管在母亲的老化和不孕的氧化应力的角色被建议了,内在的机制充分没被理解。现在的学习被设计在氧化应力下面在中期 II (MII ) 卵母细胞决定在 mitochondrial 功能和锭子稳定性之间的关系。MII 老鼠卵母细胞当面与 H2O2 被对待或渗透转变毛孔(PTP ) 的缺席块 ers cyclosporin A (CsA ) 。另外,抗氧化剂 N-acetylcysteine (NAC ) , F0/F1 synthase 禁止者 oligomycin A,线粒体 uncoupler 羰基氰化物 4-trifluoro-methoxyphenylhydrazone (FCCP ) 或加 2.5 公里 Ca2+(Th+2.5 公里 Ca2+) 的 thapsigargin 在机械学的研究被使用。卵母细胞锭子和染色体的词法分析被执行并且 mitochondrial 膜潜力(DeltaPsim ) ,细胞质的免费的钙集中([Ca2+] c ) 并且在卵母细胞以内的细胞质的 ATP 内容也是 assayed。在一时间 -- 并且 H2O2 剂量依赖者举止,锭子在卵母细胞以后被发现的 meiotic 的混乱与 H2O2 被对待,它被预告的处理与 NAC 阻止。H2O2 的管理导致了 DeltaPsim 的驱散,的增加[Ca2+] 在细胞质的 ATP 的 c 和减少铺平。到 H2O2 处理的卵母细胞的这些有害回答能被孵化前与 CsA 堵住。类似于驱散的 H2O2,两 oligomycin A 和 FCCP DeltaPsim,减少的细胞质的 ATP 内容和拆卸的 MII 卵母细胞锭子,当时高[Ca2+] c 独自没在锭子形态学上有效果。在结论,在导出线粒体的 ATP 的减少可以在氧化应力期间引起老鼠 MII 卵母细胞锭子的拆卸,大概由于 mitochondrial PTP 的开始。 | Xiao Zhang~(1,2,*) Xue Qing Wu~(1,3,*) Shuang Lu~(4,5,6) Ying Lu Guo~1 Xu Ma~(4,5,6) ~1Peking University First Hospital,Institute of Urology of Peking University,Beijing 100034,China ~2Reproductive and Genetic Center of Peking University First Hospital,Beijing 100034,China ~3Department of Obstetrics and Gynecology,Shanxi Medical University Second Hospital,Taiyuan 030000,China ~4Reproductive and Genetic Center of National Research Institute for Family Planning,Beijing 100038,China ~5Department of Genetics, Peking Union Medical College, Beijing 100038. China ~6 WHO Collaborative Center for Research in Human Reproduction,Beijing 100038,China | 2006 | Cell Research2006，16，10： | 24 |
| 6 | Epidemiological survey of cryptosporidiosis in Anhui Province China显示文摘AIM: To provide scientific evidence for prevention and controlling of cryptosporidiosis, the infection of Cryptosporidium parvum and its epidemiological characteristics were studied in some areas of Anhui Province.METHODS: The oocyst of Cryptosporidium parvum in 5421fresh stool samples from eleven areas of Anhui Provincewas tested by auramine-phenol stain and improved anti-acidstain respectively. The specific antibody of IgG, IgM and Tsubsets of 41 patients with positive Cryptosporidium parvumin stools were detected by ELISA and biotin-streptavidin(BSA) respectively.RESULTS: The total infective rate of Cryptosporidiumparvum was 1.33 % (74/5421). Among them, the positiverates of oocyst in the areas of Huaibei (1. 82 % ) and Fuyang( 1. 80 % ) were higher. The positive rates of oocyst in stoolsof infants, pupils, middle school students, collegestudents, adults, patients with diarrhea, and those withimmunodeficiency were 3.15 % (28/889), 0.82 % (9/1098), 0.82 %(9/1092), 0.83 %(8/969), 0.85 % (9/1095), 2.88 %(8/27g) and 8.33 %(3/36) % respectively. The positive rates ofoocyst in infants and the patients with diarrhea andimmunodeficiency were significantly higher than those incontrols ( P < 0.01 ). The positive rate of oocyst in maleswas similar to that in females ( P> 0.05). The positive rateof oocyst in urban areas ( 1. 13 %) was significantly lowerthan those in rural areas ( 1. 72 %, P < 0.01 ). The positiverates of specific IgG, IgM and IgG + IgM in sera of thepatients with positive oocyst in stool were 63.4 % ( 26/41 ),17.1% (7/41), 19.5 % (8/41) respectively. The numberfractions of T subsets of CD3 + , CD4 + , CD8 + and CD4 +/CD8 + of the patients were 0.66 ± 0.07, 0.44 ± 0.06, 0.28 ± 0.04 and 1. 58 ± 0.32 respectively. The difference between thepatients and the controls was significant ( P < 0. 05). Themain manifestations of the patients were subclinicalinfection, in forms of slight abdominal pain, mild diarrhea,and loose stool.CONCLUSION: There are two infection peaks in infection ofCryptosporidium parvum and its infection can he foundmore often in infants, patients with diarrhea orimmunodeficiency, and in rural areas. Subclinical infectionis the main manifestation and might he easily misdiagnosed.When the therapeutic effectiveness is low for diarrhea, theinfection of Cryptosporidium parvum should he considered,conceming their age and immune function. | Ke-Xia Wang Chao-Pin Li Jian Wang,Department of Aetiology and Immunology, School of Medicine, Huainan University of Technology,Huainan 232001,Anhui Province,China Bo-Rong Pan,Department of Oncology,Xijing Hospital,the Fourth Military Medical University,Xian,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 15 |
| 7 | Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA显示文摘Using agarose gel electrophoresis and T4-endo-deoxyribonuclease-V, which is a kind of restriction endonu-clease of cyclobutyl pyrimidine dimer (CPD), the impacts of He-Ne laser (5mW·mm-2) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08kJ·m-2·d-1) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested by T4-endodeoxyribonuclease-Y had been stimulated in the wheat cells. | HAN Rong, WANG Xunling & YUE MingSchool of Life Science, Northwest University, Xi’an 710069, China School of Life Science, Shanxi Normal University, Linfen 041004, China School of Life Science, Lanzhou University, Lanzhou 730000, China | 2002 | Chinese Science Bulletin2002，47，10： | 13 |
| 8 | Tension and Thickness Control Strategy Analysis of Two Stands Reversible Cold Rolling Mill显示文摘In order to lucubrate the rolling characteristic of the two stands reversible cold rolling mill and establish the tension and thickness control strategy, the steady-state characteristic simulating program was established using the steady-state continuous rolling theory. The influences of each factor on exit thickness under different tension control methods were analyzed. The results show that, the influence of the entry stand gap on exit thickness is significant and it changes little with different tension control methods. As a result, the entry stand gap can be used as the main control method of exit thickness, whereas rolling speed of any stand and roll gap of the exit stand can be used to adjust the inter-stand tension. The results are beneficial for thickness and tension control of the two stands reversible cold rolling mill. | LIU Guang-ming1, DI Hong-shuang2, ZHOU Cun-long1, LI Hong-cui3, LIU Jiang3 (1. College of Materials Science and Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, Shanxi, China 2. State Key Laboratory of Rolling and Automation, Northeastern University, Shenyang 110819, Liaoning, China 3. Cold Rolling Plant, Jinan Iron and Steel Group Co Ltd, Jinan 250101, Shandong, China) | 2012 | Journal of Iron and Steel Research(International)2012，19，10： | 12 |
| 9 | 山西长治八义窑试掘报告显示文摘八义窑是古代山西地区重要的民窑之一。可有关它的烧造情况,却未见文献记载。50年代,陈万里先生已报道了长治八义生产白釉红绿彩瓷①,以后不少人作过考察,但至今没有见到报告。为了进一步了解这个古瓷窑的详细情况,山西省考古研究所1996年11月,对长治八义一... | Kiln of the Jin Dynasty in Changzhi,Shanxi | 1998 | 文物世界1998，，3： | 11 |
| 10 | Information Measures of Roughness of Knowledge and Rough Sets for Incomplete Information Systems显示文摘In this paper we address information measures of roughness of knowledge and rough sets for incomplete information systems. The definition of rough entropy of knowledge and its important properties are given. In particular, the relationship between rough entropy of knowledge and the Hartley measure of uncertainty is established. We show that rough entropy of know1edge decreases monotonously as granularity of information become smaller. This gives an information interpretation for roughness of knowledge. Based on rough entropy of knowledge and roughness of rough set. a definition of rough entropy of rough set is proposed, and we show that rough entropy of rough set decreases monotonousIy as granularity of information become smaller. This gives more accurate measure for roughness of rough set. | LIANG Ji-ye, QU Kai-she Department of Computer Science, Shanxi University, Taiyuan 030006, China | 2001 | Journal of Systems Science and Systems Engineering2001，13，4： | 9 |
| 11 | Carcinogenic potential of duodenal reflux juice from patients with long-standing postgastrectomy显示文摘AIM To determine, whether study on thecarcinogenic potential of reflux juice frompatients with remote gastrectomy could clarifythe inherent relationship between duodenalreflux and gastric stump cancer.METHODS A total of 37 reflux juice samples(13 Billroth T, 24 Billroth Ⅱ ) were employed inthe present study. A two- stage transformationassay using BALB/c 3T3 cells was carried out totest the initiating or promoting activity of thesesamples.RESULTS Two of 18 (11.1%) reflux samplesexerted initiating activities, whereas 9/19(47.4%) samples enhanced the MNNG-initiafingcell transformation, suggesting the duodenalreflux juice might more frequently possess thetumor-promoter activity (P = 0. 029). In addition,there was no difference in initiating activities ofthe samples irrespective of surgical procedures(P=0.488), while Billroth Ⅱ samples exhibitedstronger tumor-promoter activity than Billroth Ⅰsamples (P = 0.027). Furthermore, the promoteractivities were well correlated with thehistological changes of the stomas (rs = 0.625,P = 0.004), but neither their cytotoxicities norinitiating activities had this correlation( Probabilities were 0.523 and 0.085,respectively).CONCLUSION The duodenal reflux juice frompatients with remote postgastrectomy did havecarcinogenic potential, and suggested thattumor-promoting activity should principallyaccount for the high incidence of gastric cancerin gastrectomy patients. In contrast, it isdifficult to explain the high stump-cancerincidence with the 'N-nitroso compounds'theory-a popular theory for the intact stomachcarcinogenesis, and it seemed to be justified tofocus chemoprevention of this cancer on thetumor-promoting potential of reflux juice. | Zhe Fu Ma~1 Zhong Yu Wang~1 Jun Ran Zhang~2 Peng Gong~1 Hai Long Chen~1 ~1Departrnent of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China ~2Department of Radiation Oncology,Massachusetts General Hospital and Harvard Medical School,Charlestown,MA 02129,USADr.Zhe Fu Ma graduated from Shanxi Medical College in 1993,nowPh.D.,M.D.in Department of Surgery,Dalian Medical University,majoring gastroenteric cancer,having 4 papers published.Correspondence to:Dr.Zhe Fu Ma.Department of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China Tel.0086-411-4720334 | 2001 | World Journal of Gastroenterology2001，7，3： | 9 |
| 12 | Effect of a single oral dose of rabeprazole on nocturnal acid breakthrough and nocturnal alkaline amplitude显示文摘AIM: To study the effect of rabeprazole (RAB) on nocturnal acid breakthrough (NAB) and nocturnal alkaline amplitude (NAKA) and to compare it with omeprazole (OME) and pantoprazole (PAN).METHODS: By an open comparative study, forty patients with active peptic ulcer were randomly assigned to receive one of the three PPIs (proton pump inhibitor) with a single oral dose. They were divided into RAB group (10 mg), OME group(20 mg) and PAN group (40 mg). Twenty healthy volunteers were enrolled to the control group (without taking any drug). Intragastric pH monitoring was then performed 1 hour before and 24 hours after the dose was given.RESULTS: No clinically undesirable signs and symptoms possibly attributed to the administration of RAB or OME and PAN were recognizable throughout the study period. All subjects completed the study according to the protocol. All data were processed by a computer using the Student ttest or t' test followed by an analysis of covariance. P<0.05 was considered to have statistical significance. The intragastric pH of NAB was significantly higher in RAB group (1.84±0.55) than in either OME group (1.15±0.31) or PAN group (1.10±0.30) (both P<0.01). RAB produced a longer sustaining time (4.65±1.22 h) on NAKA than OME (3.22±1.89 h) (P<0.05),PAN (3.15±1.92 h) (P<0.05), and the sustaining time of NAKA in RAB group was longer than that in the healthy control group (P<0.01) too. In addition, RAB produced a much higher pH on NAKA (6.41±0.45) in comparison with PAN (6.01±0.92) (P<0.05).CONCLUSION: A single oral dose of 10 mg RAB may increase the pH of NAB and shorten the sustaining time of NAB, and it may increase the pH of NAKA as well as prolong the sustaining time of NAKA. | Jin-Yan Luo Chun-Yan Niu Xue-Qin Wang You-Ling Zhu Jun Gong, Department of Gastroenterology, The Second Hospital of Xi’an Jiaotong Uuniversity, Xi’an 710004, Shanxi Province, China | 2003 | World Journal of Gastroenterology2003，9，11： | 9 |
| 13 | Conformal isoparametric hypersurfaces with two distinct conformal principal curvatures in conformal space显示文摘The conformal geometry of regular hypersurfaces in the conformal space is studied.We classify all the conformal isoparametric hypersurfaces with two distinct conformal principal curvatures in the conformal space up to conformal equivalence. | NIE ChangXiong 1,2,LI TongZhu 3,HE YiJun 4 & WU ChuanXi 5 1 Faculty of Mathematics and Computer Sciences,Hubei University,Wuhan 430062,China 2 School of Mathematical Sciences,Peking University,Beijing 100871,China 3 Faculty of Sciences,Beijing Institute of Technology,Beijing 100081,China 4 School of Mathematical Sciences,Shanxi University,Taiyuan 030006,China 5 Institute of Mathematics,Hubei University,Wuhan 430062,China | 2010 | Science China Mathematics2010，53，4： | 9 |
| 14 | The results and prognosis of different treatment modalities for solitary metastatic lung tumor from nasopharyngeal carcinoma:a retrospective study of 105 cases显示文摘Background and Objective:Nasopharyngeal carcinoma (NPC) is known for its propensity for distant metastases.Lung metastasis is one of the most important causes of death for patients with NPC.Solitary metastatic lung tumor from NPC is a distinctive group associated with a better survival.This study was to find a more effective treatment modality and prognostic factors for the group.Methods:Clinical data of 105 cases of solitary metastatic lung tumor from NPC were retrospectively analyzed.Survival rate was calculated by the Kaplan-Meier method.The difference of survival between the patients treated by different modalities was evaluated by the log-rank test.The Cox univariate and multivariate analyses of gender, age, pathologic type, stage, adjuvant chemotherapy, evaluation of treatment for NPC, disease-free interval, size of metastatic tumor, pulmonary hilar and/or mediastinal lymph node metastasis, treatment modalities, recurrent distant metastases and/or relapse of NPC were conducted.Results:The local control rate was 53.8% in chemotherapy group, 88.0% in radiotherapy +/-chemotherapy group, and 96.4% in operation +/-chemotherapy group (P<0.01).The most promising progression-free survival (PFS) and overall survival (OS) were obtained with operation +/-chemotherapy and followed by radiotherapy +/-chemotherapy.Both of them showed much better efficacy than chemotherapy (P<0.001).The Cox multivariate analysis showed that recurrent distant metastases and/or relapse of NPC affected the survival (OR=2.087, 95% CI=1.277-3.410, P=0.003).The T stage of NPC, size of metastatic tumor, hilar and/or mediastinal lymph node metastasis, and the treatment modality were independent prognostic factors.Conclusions:Operation +/-chemotherapy and radiotherapy +/-chemotherapy are better treatment of solitary metastatic lung tumor from NPC, which could improve the local control and prolong the PFS and OS.Chemotherapy is recommended for patients with higher T stage of NPC, size of metastatic tumor ≥3 cm, pulmonary hilar and/or mediastinal lymph node metastasis. | Jun Ma1,2, Zhe-Sheng Wen1,2, Peng Lin1,2, Xin Wang1,2, Fang-Yun Xie1,3 1 State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, P.R.China 2 Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R.China 3 Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R.China Department of Thoracic Surgery, Shanxi Provincial People’s Hospital, Taiyuan, Shanxi 030012, P.R.China | 2010 | Chinese Journal of Cancer2010，29，9： | 9 |
| 15 | Screening and evaluation of human single-chain fragment variable antibody against hepatitis B virus surface antigen显示文摘BACKGROUND: Phage display technology has become a vital tool in studies aimed at identifying molecules binding to a specific target. It enables the rapid generation and selection of high affinity, fully human antibody product candidates to essentially any disease target appropriate for antibody therapy. In this study, we prepared the recombinant single-chain fragment variable ( ScFv) antibody to hepatitis B virus surface antigen (HBsAg) by the phage display technology for obtaining a virus-targeting mediator. METHODS: mRNA was isolated from B-lymphocytes from a healthy volunteer and converted into cDNA. The fragment variables of heavy and light chain were amplified separately and assembled into ScFv DNA with a specially constructed DNA linker by polymerase chain reaction. The ScFv DNA was ligated into the phagmid vector pCANT-AB5E and the ligated sample was transformed into competent E. coli TG1. The transformed cells were infected with M13K07 helper phage to form a human recombinant phage antibody library. The volume and recombinant rate of the library were evaluated by bacterial colony count and restriction analysis. After two rounds of panning with HBsAg. the phage clones displaying ScFv of the antibody were selected by enzyme-linked immunosorbant assay ( ELISA) from the enriched phage clones. The antigen binding affinity of the positive clone was detected by competition ELISA. HB2151 E. coli was transfected with the positive phage clone demonstrated by competition ELISA for production of a soluble form of the anti-HBsAg ScFv. ELISA assay was used to detect the antigen binding affinity of the soluble anti-HBsAg ScFv. Finally, the relative molecular mass of soluble anti-HBsAg ScFv was measured by SDS-PAGE. RESULTS: The variable heavy ( VH ) and variable light (VL) and ScFv DNAs were about 340bp, 320bp and 750bp, respectively. The volume of the library was up to 2 × 106 and 8 of 10 random clones were recombinants. Two phage clones could strongly compete with the original HBsAb for binding to HBsAg. Within 2 strong positive phage clones, the soluble anti-HBsAg ScFv from one clone was found to have the binding activity with HBsAg. SDS-PAGE showed that the relative molecular weight of soluble anti-HBsAg ScFv was 32 kDa. CONCLUSION: The anti-HBsAg ScFv successfully produced by phage antibody technology may be useful for broadening the scope of application of the antibody. | Jian-Lin Zhang, Jian-Jin Guo, Zi-Yan Zhang, Yi-Xin Jing, Lin Zhang, Rui Guo, Ping Yan, Niu-Liang Cheng, Bo Niu and Jun Xie Department of Biochemistry and Molecular Biology, Shanxi Medical University ,Taiyuan 030001,China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 8 |
| 16 | Hydrodynamics of a Newly China-Made Jiuling Bileaflet Heart Valve Prostheses显示文摘Jiuling bileaflet prosthetic heart valve is manufactured in compliance with the national standard of P.R.C. GB12279 90. This mechanical heart valve substitute is constructed entirely of solid low temperature pyrolytic carbon. There is no graphite substrate within the leaflets or orifice. This prosthesis presents with satisfactory histocompatibility and induces little hemocoagulation around it. Objective: This study aimed to assess the hydrodynamic function of the newly China made Jiuling bileaflet heart valve prostheses. Methods: Jiuling valve has been tested in a pulsatile flow simulator in the aortic position. The testing condition was set at the pulsatile frequency of 55, 75, and 100 beats/min and a constant cardiac output of 4 L/min. The mean pressure differences, leakage volumes and closing volumes across each valve, and the effective orifice areas have been analyzed. Results: The Jiuling heart valves prosthesis had the lowest pressure difference at any given tissue annulus diameter. The mean pressure difference of all the Jiuling valves decreases with increasing the tissue annulus diameter or pulse frequency, and is in the range of below 10 mmHg. CarboMedics (CarboMedics, Inc., Austin, Tex.) is similar to that of the 21 mm and 23 mm Jiuling heart valves at the given pulse rate under 4 L/min. C L tilting disc valve (Lanzhou CarboMedics Inc., China) is similar to that of the 23 mm Jiuling heart valve. The effective orifice area of the Jiuling mechanical valve increases with increasing the tissue annulus diameter or pulse frequency. CarboMedics and C L tilting disc valve is similar to that of the 21 mm and 23 mm Jiuling heart valves. The Closing volume of the Jiuling prosthetic cardiac valves increases with increasing the tissue annulus diameter, but decreases with increasing the pulse frequency, and is in the range of below 11% (normalized by stroke volume). CarboMedics is below 16.5% of stroke volume; and C L tilting disc valve is below 7.5%. The leakage volume of the Jiuling prosthetic cardiac valves increases with increasing the tissue annulus diameter, but decreases with increasing the pulse frequency, and are in the range of below 28.9% (normalized by stroke volume). The leakage volume of CarboMedics is higher than that of the same sized 25 mm Jiuling valve, and the C L tilting disc valve is also higher than that of Jiuling valve at 4 L/min. Conclusion: For a given tissue diameter, the Jiuling valve showed significantly better hemodynamic characteristics. The material used in the construction is biocompatible, durable, and thromboresistant. The design permits light weight, low profile, and high ratio of flow area to tissue annulus diameter. | CHU Yin ping 1,CHEN Ru kun 2,FAN Yu bo 3 , PU Fang 31 Department of Thoracic & Cardiovascular Surgery Second Hospital Affiliated Shanxi Medical University, Taiyuan 030001,China 2 Second Affiliated Hospital of Zhejiang University Medical | 2000 | Chinese Journal of Biomedical Engineering(English Edition)2000，9，4： | 8 |
| 17 | Functional analysis of the Myostatin gene promoter in sheep显示文摘Compared with the understanding for the functional mechanism of the myostatin gene, little is known about the regulatory mechanism of the myostatin gene transcription and expression. To better under-stand the function of the myostatin gene promoter (MSTNpro) in the transcriptional regulation of the myostatin gene and to further investigate the transcriptional regulation mechanism of the myostatin gene, the promoter region of the myostatin gene in sheep has been cloned in our recent study (AY918121). In this study, the wild (W) type MSTNProW-EGFP vectors and E-box (E) (CANNTG) mutant (M) type MSTNProE(3+5+7)M-EGFP vectors were constructed and the transcriptional regulation activities were compared by detecting the fluorescent strength of EGFP (enhanced green fluorescent protein) in C2C12 myoblasts (or myotubes) and sheep fibroblasts transfected with the vectors. Results showed that the 0.3―1.2 kb sheep myostatin promoter could activate the transcription and expression of EGFP gene in C2C12 myoblasts to different extent and the 1.2 kb promoter was the strongest. However, fluorescence was not observed in the sheep fibroblasts transfected with the 1.2 kb sheep myostatin promoter. These results suggested that the specific nature of the myostatin gene expression in skeletal muscle was attributed to the specific nature of the myostatin promoter activity. The increasing growth density of C2C12 myoblasts inhibited the transcriptional regulation activity of the wild type sheep my-ostatin promoter by a mechanism of feedback. The transcriptional regulation activity of the 1.2 kb wild type sheep myostatin promoter increased significantly after C2C12 myoblasts were differentiated, while the activity of 1.2 kb E(3+5+7)-mutant type myostatin promoter had no obvious change. This result suggested that MyoD may be responsible for the difference of the myostatin gene transcription and expression between growing and differentiating conditions by binding to E-box of the myostatin promoter. | DU Rong1,2, AN XiaoRong1, CHEN YongFu1 & QIN Jian3 1 State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China 2 College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China 3 College of Modern Education Technology, Shanxi Agricultural University, Taigu 030801, China | 2007 | Science China(Life Sciences)2007，50，5： | 7 |
| 18 | Construction of recombinant plasmid and prokaryotic expression in E. Coli and biological activity analysis of human placenta arresten gene显示文摘BACKGROUND: The proliferation and metastasis of cancers depend on angiogenesis. This property provides the feasibility for the treatment of cancer by inhibition of angiogenesis, and many angiogenic inhibitors have been demonstrated to effectively inhibit angiogenesis and consequently the growth of solid cancer. As for the newly identified angiogenesis inhibitor, arresten, some studies have found its high activity on restrainting tumor vessel. This study was to assess the anti-angiogenic activity of arresten. METHODS: The arresten gene was obtained from a healthy puerpera’s placenta tissue by the reverse transcriptase-polymerase chain reaction (RT-PCR) method, and molecular cloning to prokaryotic expression plasmid pBV220 by recombination strategy. The prokaryotic expression plasmid pBV220/arr was identified by restriction enzyme digestion and sequenced. The pBV220/arr was transformed into E. coli JM109, DH5α, BL21 and BL21 (DE3) by the CaCl2 transformation method. The arresten expression level was detected by SDS-PAGE. The expressed product was purlfled, re-naturalized and detected for its biological activity of inhibiting the angiogenesis of chorioallantoic membrane (CAM). RESULTS: The arresten gene was cloned and pBV220/arr was constructed. The arresten expression level of protein was highly increased after pBV220/arr was transformed into E. coli BL21 (DE3). SDS-PAGE showed that the expressed arresten proteins were mainly inclusion bodies and had a molecular weight of 26 kDa. The expressed arresten protein showed evident biological activities. CONCLUSIONS: The successful construction of recombinant plasmid pBV220/arr and the effective expression in E. coil have laid a foundation for further study of its anti-angiogenic function and may pave the way for future antitumor application. | Jin-Ping Zheng, Hai-Ying Tang, Xian-Jiu Chen, Bao-Feng Yu, Jun Xie and Tang-Chun Wu Department of Toxicology (and Department of Biochemistry and Molecular Biology Shanxi Medical University, Taiyuan 030001, China: Institute of Occupational Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 7 |
| 19 | THE CHARACTERISTIC OF EVENTUALLYUNIFORMLY CONTINUOUS C0 SEMIGROUPSON A HILBERT SPACE显示文摘A full characterization is given, in terms of the resolvent R(λ; A) of the infinitesimal generator A of a C0 semigroup T(t) on a Hilbert spaced which assures the continuity for t > t0 (t0 0) of T(t) in the uniform operator topology. | ZHANG Lianping (Department of Mathematics, Shanxi University, Taiyuan 030006, China) | 1999 | Systems Science and Mathematical Sciences1999，12，1： | 7 |
| 20 | A Method for the Environmental Quality Assessment of Surface Water Based on Artificial Neural Networks | YANG Guo\|dong\+1,\ PAN Da\|feng\+2,\ FAN Ying\|fang\+3 1.Dep. of Environmental Science, Shanxi Uni., Taiyuan 030006, China 2.Shanxi Academy of Agriculture Science, Taiyuan 030031, China 3.Institute of Molecular Science, Shanxi Uni., Taiyuan 030006, C | 2000 | Systems Science and Systems Engineering2000，10，1： | 7 |
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