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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Enhancement of glioblastoma radioresponse by a selective COX-2 inhibitor celecoxib: inhibition of tumor angiogenesis with extensive tumor necrosis显示文摘 | Kang KB Wang TT Woon CT Cheah ES Moore XL Zhu C Wong MC | 2007 | 中国神经肿瘤杂志2007，5，2： | 8 |
| 3 | Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Yan HX Wang K Wu MC | 2006 | 第二军医大学学报2006，27，5： | 6 |
| 4 | Assessing cultural competency skills in gastroenterology fellowship training显示文摘AIM:To assess and teach cultural competency skills at the fellowship training level through the use of objective structured clinical examinations(OSCEs).METHODS:We revised four scenarios to infuse a specific focus on cross-cultural care,and to render them appropriate for gastroenterology fellows.Three are discussed here:(1)Poor Health Literacy;(2)Disclosing/Apologizing for a Complication to a Patient Who Mistrusts the Healthcare System;and(3)Breaking Bad News to a Fatalistic Patient.A fourth case emphasizing shared decision-making will be described elsewhere.Four stations were completed by fellows and observed live by four faculty members,and the fellows’performance was assessed.RESULTS:Eleven fellows from four programs participated in the four OSCE.In the'Poor Health Literacy'case,18%(2/11)of participants recognized that the standardized patient(SP)had below-basic health literacy.None successfully evaluated the SP’s reading skills in a culturally-sensitive manner.In'Disclosing/Apologizing for a Complication',4/11(36%)personally apologized for the complication.1/11 recognized the SP’s mistrust of the medical system.With'Breaking Bad News',27%(3/11)explored the patient’s values to identify her fatalistic beliefs.CONCLUSION:OSCEs can be used to assess deficiencies in culturally-competent care at the fellowship level.OSCEs also afford fellowships the opportunity to inform future training curricula. | Sophie Balzora Benjamin Abiri Xiao-Jing Wang James Mc Keever Michael Poles Sondra Zabar Colleen Gillespie Elizabeth Weinshel | 2015 | World Journal of Gastroenterology2015，21，6： | 2 |
| 5 | Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation显示文摘背景在成年哺乳动物的视网膜的神经发生通常被废除,并且这显示再生 capacity.Despite 的视网膜缺乏这,有在保留学习在调查二药,皮质脂酮和 paroxetine 的效果瞄准了的在场的神经原的 potential.The 的睫的上皮的nestin积极的房间的一张小人口,在老鼠是的睫的 body.Methods 成年人 Sprague-Dawley 的房间增长上给车辆,皮质脂酮, paroxetine ,或两个 | WANG Hua LAU Benson WM YAU Suk-yu LI Suk-yee LEUNG Nelson WANG Ning-li TANG Siu-wa LEE Tatia MC SO Kwok-fai | 2010 | Chinese Medical Journal2010，，10： | 2 |
| 6 | Genetic contribution of the endothelial constitutive nitric oxide snythase gene to plasma nitric oxide levels显示文摘 | Mahaney MC Sim AS | 1997 | Arterioscler Thromb Vasc Biol1997，17，11： | 1 |
| 7 | Systematic review of the impact of beta blockers on mortality and hospital admission in heart failure 显示文摘 | Shibata MC Flather MD Wang D | 2001 | Eur J Heart Fail2001，3，3： | 1 |
| 8 | CD20-specific adoptive immu- notherapy for lymphorna using a chimeric antigen receptor with both CD28 and 4-1BB dornains: pilot clinical trial results显示文摘 | Till BG Jensen MC Wang J | 2012 | Blood2012，119，17： | 1 |
| 9 | Long-term cognitive andmotordeficits after enterovirus 71 brainstem encephalitis in children显示文摘 | Huang MC Wang SM Hsu YW | 2006 | Pediatrics2006，118，6： | 1 |
| 10 | Adoptive immunotherapy for indolent non-Hodgkin lymphoma and mantle cell lymphoma using genetically modified autologous CD20-specific T cells显示文摘 | Till BG Jensen MC Wang J | 2008 | Blood2008，112，： | 1 |
| 11 | Oral health knowledge, attitudes, and practices of Chinese adults显示文摘 | Lin HC Wong MC Wang ZJ | 2001 | J Dent Res2001，80，5： | 1 |
| 12 | Diet in midpuberty and sedentary activity in prepuberty predict peak bone mass 显示文摘 | Wang MC Crawford PB Hudes M | 2003 | Am J Clin Nutr2003，2，： | 1 |
| 13 | A mechanism of cell survival:sequestration of Fas by the HGF receptor Met显示文摘 | Wang X DeFrances MC Dai Y | 2002 | Mol Cell2002，9，2： | 1 |
| 14 | Efficacy of dilute betadine solution imigation in the prevention of postoperative infection of spinal surgery显示文摘 | Cheng MT Chang MC Wang ST | 2005 | spine2005，，： | 1 |
| 15 | Nitric oxide stress resistance in porphyromonas gingivalis is mediated by a putative hy- droxylamine reductase显示文摘 | Boutrin MC Wang C Aruni W | 2012 | J Bacteriol2012，194，6： | 1 |
| 16 | Nitric oxide stress resistance in Porphyromonas gingivalis ismediated by a putative hydroxylamine reductase显示文摘 | Boutrin MC Wang C Aruni W | 2012 | J Bacteriol2012，194，6： | 1 |
| 17 | Microfabricatedneedles for transdermal delivery of macmmolecules and nanoparticles, fabrication methods and transport studies显示文摘 | Mc?dlister D V Wang P M Davis S P | 2003 | Proc Natl Acad Sci2003，100，13： | 1 |
| 18 | Effects of Yucca schidigera extract on fermentation and degradation of steroidal saponins in the rumen simulation technique(RUSITEC)显示文摘 | Wang Y Mc Allister T A Newbold C J | 1998 | Animal Feed Science and Technology1998，74，： | 1 |
| 19 | Concentration and distribution of sialic acid in human milk and infant formulas显示文摘 | Wang B Brand-Miller J Mc Veagh P | 2001 | American Journal of Clinical Nutrition2001，74，4： | 1 |
| 20 | Albumin and fibrinogen adsorption on PU-PHEMA surfaces显示文摘 | Wang D Ji J | 2003 | Biomaterials2003，24，12： | 1 |
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