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| 1 | Dual-color deep-tissue three-photon microscopy with a multiband infrared laser显示文摘Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology.Threephoton(3P)microscopy with excitation in the short-wavelength infrared(SWIR)water transparency bands at 1.3 and 1.7μm opens up new opportunities for deep-tissue imaging.However,novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach.Here,we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7μm that has characteristics optimized for 3P microscopy:sub-70 fs duration,1.25 MHz repetition rate,and μJ-range pulse energy.In turn,we achieve simultaneous 3P excitation of green fluorescent protein(GFP)and red fluorescent proteins(mRFP,mCherry,tdTomato)along with third-harmonic generation.We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain,chick embryo spinal cord,and live adult zebrafish brain,with an improved signal-to-background ratio compared to multicolor twophoton imaging.This development opens the way towards multiparametric imaging deep within scattering tissues. | Khmaies Guesmi Lamiae Abdeladim Samuel Tozer Pierre Mahou Takuma Kumamoto Karolis Jurkus Philippe Rigaud Karine Loulier Nicolas Dray Patrick Georges Marc Hanna Jean Livet Willy Supatto Emmanuel Beaurepaire Frédéric Druon | 2018 | Light(Science & Applications)2018，7，1： | 3 |
| 2 | HPV E6/E7 mRNA原位杂交诊断宫颈低级别鳞状上皮内病变显示文摘宫颈低级别鳞状上皮内病变(LSIL)[亦称宫颈上皮内瘤变,1级(CIN1)]可带来相当大的诊断挑战,并且与观察者之间重复性较差和过诊断有关。此外,一些辅助性检测项目,如p16免疫组化检查在解析LSIL与正常/反应性鳞状上皮病变的差异作用不大。人类乳头状瘤病毒(HPV)RNA原位杂交(ISH)检测在LSIL的诊断中可能是一种较好的辅助诊断方法,但目前这方面尚没有较大宗病例的研究。 | 解建军 张仁亚 Mills A M Coppock J D Willis B C | 2018 | 临床与实验病理学杂志2018，34，11： | 2 |
| 3 | Inducible cyclooxygenase may have anti-inflammatory properties 显示文摘 | Gilroy DW Colville Nash PR Willis D | 1999 | Nat Med1999，5，6： | 1 |
| 4 | Retention of endothelium dependent vasodilatory responses in canine coronary arteries following cryopreservation显示文摘 | Ku D D Willis W L Caulfied J B | 1990 | Cryobiology1990，27，： | 1 |
| 5 | Phase Size/Composition Dependence in Irmni~ible Blends: Experimental and Theoretical Considerations显示文摘 | Favis B D Willis J M | 1990 | J Pol~n Sci Part B: Polym Phys1990，28，： | 1 |
| 6 | Rapid detection of influenza A and B viruses in clinical specimens by LightCycler real-time RT-PCR显示文摘 | Smith A B Mock V Melear R Colarusso P Willis D E | 2003 | J Clin Virol2003，28，： | 1 |
| 7 | Na,K-ATPase beta1-subunit increases the translation efficiency of the alpha1-subunit in MSV-MDCK cells显示文摘 | Rajasekaran SA Gopal J Willis D | 2004 | Mol Biol Cell2004，15，7： | 1 |
| 8 | Endogenous on cogenic K-ras (G12D) stimulates proliferation and widespread neoplastic and developmental defects 显示文摘 | Tuveson D A Shaw A T Willis N A | 2004 | Cancer Cell2004，5，4： | 1 |
| 9 | Regulation of Tabtoxin Production by the lemA Gene in Pseudomonas syringae显示文摘 | BARTA T M Kinscherf T G Willis D K | 1992 | J Bacteriol1992，174，9： | 1 |
| 10 | Excitatory amino acid receptor involvement in peripheral nociciptive transmission in rats 显示文摘 | LAWAND N B WILLIS W D WESTLUND K N | 1997 | Eur J Pharmacol1997，324，23： | 1 |
| 11 | The elastic coefficients of the theory of consolidation显示文摘 | BIOT M A WILLIS D G | 1957 | ASME J Appl Mech1957，24，： | 1 |
| 12 | The elastic coefficients of the theory of consolidation显示文摘 | BIOT M A WILLIS D G | 1957 | Journal of Applied Mechanics1957，24，4： | 1 |
| 13 | Spatial electric load forecasting:a tutorial review显示文摘 | Willis H L Northcote-Green J E D | 1983 | Proceedings of the IEEE1983，71，2： | 1 |
| 14 | Atypically low rate of eytochrome b evolution in the scleractinian coral genus acropora显示文摘 | MADELEINE J H WILLIS B L MILLER D J | 1999 | The Royal Society1999，266，： | 1 |
| 15 | Follicular dynamics in the polycystic ovary syndrome显示文摘 | Mason H Willis D | 2000 | Mol Cell Endocrinol2000，163，12： | 1 |
| 16 | Maximum-likelihood analysis of molecular adaptation in abalone sperm lysin reveals variable selective pressures amongLineages and sites显示文摘 | Yang Z Willie J S Victor D V | 2000 | Mol Biol Evol2000，17，10： | 1 |
| 17 | Image interpolation by pixel level data-dependent triangulation显示文摘 | SU D WILLIS P | 2004 | Computer Graphics Forum2004，23，2： | 1 |
| 18 | Nucleotide Sequence of an Immediate-early FV3 gene 显示文摘 | Willis D B Foglesong D Granoff A | 1984 | J Virol1984，53，： | 1 |
| 19 | Leac-hing of nitrate,atrazine,and metribuzin from sugarcanein southern louisiana显示文摘 | Southwick L M Willis G H Johnson D C | 1995 | Journal of Environmental Qual-ity1995，24，68： | 1 |
| 20 | Validity of the taylor-sedov theory for studying laser-induced phase explosion and shock waves显示文摘 | Hendijanifard M Willis D A | 2015 | Journal of Nanoscience and Nanotechnology2015，15，4： | 1 |
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