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| 1 | Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture显示文摘To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21―40 years old, 41―60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula “ log2T D = t logN t ? logN 0” was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These find- ings suggested that a higher level of hADAS cells replication activity was found in the younger dona- tors, and they represent novel and valuable seed cells for studies of tissue engineering. | LEI Lei, LIAO WeiMing, SHENG PuYi, FU Ming, HE AiShan & HUANG Gang Department of Orthopedics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China | 2007 | Science China(Life Sciences)2007，50，3： | 25 |
| 2 | 青光眼和近视眼的分子机制及干预研究显示文摘本文扼要报道青光眼和近视的病因、发病机理及干预研究的主要结果。通过对GZ.1、PN.1青光眼家系及散发性青光眼的研究,发现Myocilin基因的Pro370Leu突变,导致蛋白质错误折叠,内质网应激。我们提出了青光眼属于'蛋白质构象病'、'线粒体病'的学说,并研究了靶向siRNA术后抗疤痕、视神经保护药物、干细胞替代治疗及药物缓释系统在青光眼治疗中的应用,为实现青光眼'分期靶点干预'奠定了基础。在近视的研究中,从灵长类恒河猴自然动物模型的构建着手,着重研究光学离焦与近距离负荷对青少年近视发生发展的影响,并且深入探讨了近视的视网膜与中枢神经机制,同时提出了光学干预近视的新策略。这些围绕青光眼和近视基本问题进行的系列研究,将可能为其诊治提供新的模式。 | 葛坚 | 2010 | 中山大学学报(医学科学版)2010，31，2： | 15 |
| 3 | Putative epidermal stem cell convert into corneal epithelium-like cell under corneal tissue in vitro显示文摘Rhesus putative epidermal stem cells are being investigated for their potential use in regenerative corneal epithelium-like cells, which may provide a practical source of autologous seed cells for the construction of bioengineered corneas. The goal of this study was to investigate the potential of epi-dermal stem cells for trans-differentiation into corneal epithelium-like cells. Rhesus putative epidermal stem cells were isolated by type IV collagen attachment method. Flow cytometry analysis, immuno-histology and RT-PCR were conducted to identify the expression of specific markers (β1, α6 integrin, K15, K1/K10, K3/K12 and CD71) on the isolated rapid attaching cells. The isolated cells were cocultured with human corneal limbal stroma and corneal epithelial cells. After coculture, the expression of the same specific markers was evaluated in order to identify expression difference caused by the coculture conditions. K3/K12 expression was analyzed in coculture cells on day 2, 4, 6, 8 and 10. Putative epi-dermal stem cells in conditioned culture media were used as control. Putative epidermal stem cells were predominant in rapid attaching cells by type IV collagen attachment isolation. Before being co-cultured, the rhesus putative epidermal stem cells expressed K15, α6 and β1 integrin, but no CD71, K1/K10 and K3/K12. After coculture, these cells expressed K3/K12 (a marker of corneal epithelial cells), K15 and β 1 integrin, but no K1/K10. Cells being not coculture converted into terminally differentiated cells expressing K1/K10. These results indicate that rhesus putative epidermal stem cells can trans-differentiate into corneal epithelium-like cells and, therefore, may have potential therapeutic application as autologous seed cells for the construction of bioengineered corneas. | GAO Nan1, WANG ZhiChong1, HUANG Bing1, GE Jian1, LU Rong1, ZHANG KeFei1,2, FAN ZhiGang1, LU Li1, PENG Zhan1 & CUI GuangHui1 1 Key Laboratory of Ophthalmology (Sun Yat-sen University), Ministry of Education Zhongshan Ophthalmic Center, Guangzhou 510060, China 2 Liwan Hospital of Guangzhou Medical College, Guangzhou 510165, China | 2007 | Science China(Life Sciences)2007，50，1： | 8 |
| 4 | 模拟人角膜内皮细胞微环境诱导人胚胎干细胞分化为角膜内皮细胞的研究进展显示文摘角膜内皮供体不足成为开展角膜内皮移植等手术的主要问题,生物工程角膜为角膜内皮移植的开展提供了解决途径.人胚胎干细胞(ESCs)诱导分化出功能性角膜内皮细胞后可以在一定程度上缓解角膜供体不足,尤其是角膜内皮供应不足的现状.目前理想可行的方法是体外模拟角膜内皮细胞的发育过程,将人ESCs诱导分化为神经嵴干细胞(NCSCs),再利用生长因子和细胞外基质构建细胞生长的微环境,制备适当的条件培养基以诱导NCSCs分化为角膜内皮细胞.目前NCSCs相关的诱导机制尚不十分清楚,有待进一步研究.本文就微环境对角膜内皮细胞发育过程的影响,以及诱导人ESCs分化为角膜内皮细胞等方面的研究进展进行综述。 | 孙琳 吴欣怡 | 2015 | 中华实验眼科杂志2015，33，9： | 5 |
| 5 | Importance of the stem cell microenvironment for ophthalmological cell-based therapy显示文摘Cell therapy is a promising treatment for diseases that are caused by cell degeneration or death. The cells for clinical transplantation are usually obtained by culturing healthy allogeneic or exogenous tissue invitro. However, for diseases of the eye, obtaining the adequate number of cells for clinical transplantation is difficult due to the small size of tissue donors and the frequent needs of long-term amplification of cells in vitro, which results in low cell viability after transplantation. In addition, the transplanted cells often develop fibrosis or degrade and have very low survival. Embryonic stem cells(ESCs) and induced pluripotent stem cells(i PS) are also promising candidates for cell therapy. Unfortunately, the differentiation of ESCs can bring immune rejection, tumorigenicity and undesired differentiated cells, limiting its clinical application. Although i PS cells can avoid the risk of immune rejection caused by ES cell differentiation post-transplantation, the low conversion rate, the risk of tumor formation and the potentially unpredictable biological changes that could occur through genetic manipulation hinder its clinical application. Thus, the desired clinical effect of cell therapy is impaired by these factors. Recent research findings recognize that the reason for low survival of the implanted cells not only depends on the seeded cells, but also on the cell microenvironment, which determines the cell survival, proliferation and even reverse differentiation. When used for cell therapy, the transplanted cells need a specific three-dimensional structure to anchor and specific extra cellular matrix components in addition to relevant cytokine signaling to transfer the required information to support their growth. These structures present in the matrix in which the stem cells reside are known as the stem cell microenvironment. The microenvironment interaction with the stem cells provides the necessary homeostasis for cell maintenance and growth. A large number of studies suggest that to explore how to reconstruct the stem cell microenvironment and strengthen its combination with the transplanted cells are key steps to successful cell therapy. In this review, we will describe the interactions of the stem cell microenvironment with the stem cells, discuss the importance of the stem cell microenvironment for cell-based therapy in ocular diseases, and introduce the progress of stem cell-basedtherapy for ocular diseases. | Peng-Xia Wan Bo-Wen Wang Zhi-Chong Wang | 2015 | World Journal of Stem Cells2015，7，2： | 3 |
| 6 | 兔角膜缘干细胞体外分离、培养和生物学鉴定的实验研究显示文摘通过体外培养兔角膜缘干细胞,观察其生物学特性,建立兔角膜缘干细胞的体外培养方法。方法0.25%胰蛋白酶消化角膜缘组织,用含15%胎牛血清的DMEM和F12(1:1)的培养液(DF)对兔角膜缘干细胞进行体外培养,形态学观察,培养的细胞早期使用AEl/AE3、晚期使用AE5角蛋白特异的单克隆抗体)作细胞免疫化学鉴定。结果:原代培养细胞48h后开始贴壁,部分细胞由圆形变为卵圆形或长梭形;10~14d形成单层,细胞呈圆形、卵圆形,类角膜上皮细胞;细胞传到第5代左右开始出现老化状态;免疫细胞化学染色:培养的细胞早期AEl/AE3呈阳性而少部分细胞AE5呈阳性,培养的细胞晚期AE5呈阳性。结论:本实验初步建立了一套兔角膜缘干细胞的体外培养方法。 | 顾国贞 白靓 王芳 周余来 成岩 颜炜群 | 2007 | 现代生物医学进展2007，7，4： | 2 |
| 7 | 中国干细胞研究的现状、挑战和机遇显示文摘干细胞研究是过去十年中生物医学研究中最为热门的领域之一。我国的干细胞研究取得了很大的进步,同时也遇到不少问题。本文回顾了过去几年中我国干细胞研究的发展情况,从科研投入、硬件建设、人才队伍、科研成果、公共资源、法律法规、伦理准则、知识产权等多个方面,对我国干细胞研究的现状、面临的机遇和挑战进行了思考和分析,旨在对我们的工作有一个清醒的认识,以便能更好地部署我们下一步的工作。 | 徐国彤 | 2007 | 前沿科学2007，1，4： | 2 |
| 8 | 乳牙牙根吸收情况对乳牙牙髓干细胞提取结果的影响显示文摘背景:间充质干细胞来源于多种组织,如骨髓、牙髓、胎盘、脐带和脂肪等,其中乳牙牙髓间充质干细胞具有强大的干性和生物学活性,无排异反应,免疫调节能力强,是非常优秀的生物治疗细胞来源之一。由于乳牙牙髓间充质干细胞取材方便,适宜于大规模培养,因此为牙髓干细胞的产业化奠定了良好基础。目的:观察乳牙牙根吸收情况对牙髓干细胞提取结果的影响,为进一步判断乳牙源齿的拔除时机提供有效的依据。方法:采集173例小学生173颗源齿,年龄7-9岁。在采集前对源齿进行根尖X射线片或曲面体层片检查,检查标准以及方法均遵照世界卫生组织专业检查标准。按牙根吸收情况将乳牙分为牙根吸收1/3、牙根吸收1/2、牙根吸收2/3、牙根完全吸收(髓室底完整)、自然脱落的乳牙5种情况,采集的源齿在拔牙离体后7 s内放入培养液中,于2-4℃的冰箱中保存,24 h内由专业的冷链物流送至北京口腔干细胞库进行乳牙牙髓干细胞的提取和保存,对检测结果进行统计分析。结果与结论:①牙根吸收1/3的源齿32颗,牙髓干细胞提取成功30颗,成功率94%,继承恒牙异位萌出12颗,平均萌出时间(2.19±0.18)个月;②牙根吸收1/2的源齿35颗,牙髓干细胞提取成功32颗,成功率92%,继承恒牙异位萌出11颗,平均萌出时间(1.89±0.13)个月;③牙根吸收2/3的源齿59颗,牙髓干细胞提取成功54颗,成功率92%,继承恒牙异位萌出8颗,平均萌出时间(1.42±0.12)个月;④牙根完全吸收但髓室底完整的源齿37颗,牙髓干细胞提取成功34颗,成功率92%,继承恒牙异位萌出2颗,平均萌出时间(1.03±0.15)个月; ⑤自然脱落的乳牙10颗,牙髓干细胞提取成功0颗,继承恒牙异位萌出4颗,平均萌出时间(0.65±0.23)个月;⑥结果表明,自然脱落的乳牙为无牙髓的牙齿,无干细胞;乳牙在Ⅱ度松动以内,牙根吸收2/3以上或牙根完全吸收但髓室底完整存在的情况下,提取成功率高,且对恒牙的萌出无太大影响,为最佳的乳牙源齿采集时机。 | 邹智群 杨晓宇 赵秀兰 马玉实 孙境辰 | 2018 | 中国组织工程研究2018，22，5： | 1 |
| 9 | 生物工程角膜种子细胞的研究进展显示文摘生物工程角膜的构建需要充足的角膜上皮及内皮种子细胞来源。由于人角膜上皮及内皮细胞增殖能力有限,难以通过原代培养的方法获取大量种子细胞。干细胞来源充足,并且具有多能分化潜能,是优良的种子细胞来源,是目前生物工程角膜研究的主要内容之一。干细胞定向诱导分化的研究取得了较大的进展,常用的诱导干细胞分化的方法有细胞因子添加法、基因转染法、细胞共培养法及条件培养基法等。文中就目前将干细胞诱导分化为角膜上皮及内皮细胞的方法做一综述。 | 鞠成群 吴欣怡 | 2017 | 中国实用眼科杂志2017，35，2： | 0 |
| 10 | 干细胞技术与牙齿再生的研究进展显示文摘牙齿是一个复杂的生物器官,由多种组织组成,包括牙釉质、牙本质、牙骨质和牙髓,牙齿脱落是最常见的器官衰竭[1]。牙齿及其支持组织是由上下颌突和额鼻突的外胚层及外胚间叶细胞发育而来,其发育是一个长期、复杂的生物学过程,包括细胞与细胞、上皮与间充质的相互作用,细胞分化,形态发生,组织矿化和牙齿的萌出, | 刘晓 何颖 朴正根 | 2015 | 中国美容医学2015，24，10： | 0 |
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