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| 1 | Expressions of cysteine-rich61,connective tissue growth factor and Nov genes in hepatocellular carcinoma and their clinical significance显示文摘AIM: To investigate the expression of cysteine-rich61 (Cyr61),connective tissue growth factor (CTGF) and nephroblastomaoverexpressecl gene (Nov) in hepatocellular carcinoma (HCC),and to evaluate the relationship between Cyr61, CTGF and Nov genes expression with invasion and metastasis of HCC.METHODS: Thirty-one HCC specimens were divided into small hepatocellular carcinoma (SHCC), nodular hepatocellular carcinoma (NHCC), solitary large hepatocellular carcinoma (SLHCC) according to their diameter and number of nodes. Reverse transcription polymerse chain reaction (RT-PCR) was used to detect the mRNA expression levels of Cyr61, CTGF and Nov genes in 31 resected specimens of hepatocellular carcinoma and para-cancerous normal liver tissues semi-quantitatively and the relation between their expression levels and clinical pathological parameters were compared.RESULTS: The expressions of Cyr61 and CTGF mRNA in carcinoma tissues were significantly higher than those in para-cancerous normal liver tissues (P<0.01). The expressions of Cyr61 and CTGF mRNA in HCC with venous invasion were higher than those in HCC without venous invasion. CTGF expression in HCC Edmondson's grade Ⅲ-IV was significantly higher than that in HCC Edmondson's grade I-II (P = 0.022). There was no obvious correlation between Nov mRNA and clinical-pathological features.Compared to NHCC, SLHCC had better cell differentiation,easier capsule formation, less microscopic venous invasion,milder liver cirrhosis. The expressions of Cyr61 and CTGF mRNA in NHCC were significantly higher than those in SLHCC and SHCC.CONCLUSION: Cyr61 and CTGF genes may play an important role in hepatocellular carcinogenesis and correlate with recurrence and metastasis of hepatocellular carcinoma.SLHCC has better biological behaviors than NHCC. | Zhi-JunZeng Lian-YueYang XiangDing WeiWang | 2004 | World Journal of Gastroenterology2004，10，23： | 28 |
| 2 | Efficacy of postoperative antiviral combined transcatheter arterial chemoembolization therapy in prevention of hepatitis B-related hepatocellular carcinoma recurrence显示文摘 | YAN Qiang NI Jun ZHANG Guo-lei YAO Xing YUAN Wen-bin ZHOU Lin ZHENG Shu-sen | 2013 | Chinese Medical Journal2013，，5： | 16 |
| 3 | 表皮生长因子及其受体与肝癌的关系显示文摘EGF、EGFR具有广泛的生理作用,体内分布广泛,在肝癌的形成、复发及转移过程中,EGF、EGFR与Ca2+通道、Na+ H+通道、磷脂酰肌醇信息传递通道、基因表达异常、肿瘤血管及间质的形成关系密切,提示了治疗肝癌的新方向。现就EGF、EGFR与肝癌的形成、转移、复发的关系及其在临床中的应用前景作一综述。 | 王文奇 徐瀚峰 刘倩 | 2005 | 国外医学(肿瘤学分册)2005，32，3： | 12 |
| 4 | Tip30基因表达对肝癌细胞侵袭转移能力的影响显示文摘目的:分析Tip30表达对肝癌细胞侵袭转移能力的影响。方法:聚乳酸-羟乙酸(polylactic-co-glycolic acid,PLGA)纳米粒介导腺相关病毒质粒pAAV-Tip30真核表达质粒转染HepG2细胞。RT-PCR检测基质金属蛋白酶(matrix metalloproteinases,MMP)-2,MMP-9和表皮生长因子受体(epidermal growth factor receptor,EGFR)mRNA水平;Western印迹检测MMP-2和MMP-9蛋白表达水平;Transwell法检测肝癌细胞迁移能力;四氮唑盐(MTS)法和细胞集落形成实验检测细胞增殖能力;细胞-细胞黏附试验、细胞-基质黏附实验检测Tip30表达对肝癌细胞黏附能力的影响。结果:过表达Tip30基因的HepG2肝癌细胞明显降低了MMP-2,MMP-9的mRNA表达和蛋白的翻译;肝癌细胞的增殖、锚定非依赖性生长及肝癌细胞迁移能力均明显受到抑制。结论:体外实验研究证明Tip30基因可抑制肝癌细胞侵袭转移的能力。 | 李浩 吴金术 蒋波 梁中书 | 2014 | 中南大学学报(医学版)2014，39，8： | 12 |
| 5 | 动脉化疗栓塞术预防肝癌根治性切除术后肿瘤复发的成本效果分析显示文摘目的建立肝细胞癌(简称肝癌)根治性切除术后预防性动脉化疗栓塞术(transcatheter arterial chemoembolization,TACE)治疗的卫生经济学评价模型。方法 140例肝癌患者根治性切除术后分为TACE组和非TACE组(即门诊随访组),收集两组治疗方案直接医疗成本和治疗效果(复发率及无瘤生存时间),进行成本效果分析、增量成本效果分析和敏感度分析。结果 (1)40例非TACE组患者门诊总平均费用为7 121.44元/人;100例TACE组患者复发前总费用为2 8250.45元/人。(2)非TACE组和TACE组的1、2、3、4、5年复发率比较差异无统计学意义(P>0.05);两组无瘤生存时间比较差异无统计学意义(P=0.322)。(3)非TACE组的成本效果比为389.15元/月,TACE组为1 278.30元/月,增量成本效果比为5 560.27元/月。(4)敏感度分析中非TACE组的成本度效果比为350.84元/月,TACE组为1 215.81元/月,增量成本效果比为5 381.35元/月。结论从卫生经济学角度,预防肝癌术后复发,预防性TACE并不是最优方案,至少不是每个患者都应该或都适合做,在相同的效果下(复发率、无瘤生存时间)门诊随访治疗可能更经济。 | 李仕来 万裴琦 彭涛 肖开银 苏铭 尚丽明 徐邦浩 苏智雄 叶新平 彭宁 黎乐群 | 2015 | 肿瘤防治研究2015，42，4： | 10 |
| 6 | JAK/STAT信号通路中关键分子基因多态性与肝细胞癌易感性的关系显示文摘目的探讨宿主JAK/STAT信号通路中关键分子基因多态性与肝细胞癌(HCC)易感性的关系。方法采用病例对照研究,用质谱法对367例诊断明确的HBsAg阳性HCC患者(HCC组)和性别、年龄匹配的367例对照的IL-6(rs1800796,-572C〉G)、STAT3(rs744166,426312T〉C;rs3816769,+42240T〉C;rs6503695,+40980T〉C)、EGFR(rs11543848,+142530A〉G)、mTOR(rs7211818,+170278A〉G;rs9674559,+196983A〉G;rs11653499,+65678G〉A)进行多态性检测。单因素分析确定各位点多态性比值比(OR)和95%可信区问(凹)。结果IL-6、STAT3、EGFR、mTOR的8个多态性位点各基因型在HCC组和对照组中分布的差异无统计学意义(P〉0.05)。按性别分层后,在女性中,与TT基因型相比,携带STAT3+26312CC、+42240CC、十40980CC基因型个体患HCC的危险陛降低(OR=0.192,95%CI=0.047~0.784;OR=0.180,95%CI:0.045~0.725;OR=0.198,95%CI:0.049~0.806)。与AA基因型相比,EGFR+142530(AG+GG)基因型降低女性患HCC的风险(OR=0.422,95%CI:0.179-0.994)。结论IL-6(rs1800796)、mTOR(rs7211818,rs9674559,rs11653499)多态性与HCC易感性无关;女性携带STAT3(rs744166,rs3816769,rs6503695)CC基因型及EGFR(rs11543848)(AG+GG)的个体患HCC的风险降低,但还需更大样本验证。 | 谢佳新 殷建华 张琪 蒲蕊 张玉伟 鹿文英 曹广文 | 2012 | 中华流行病学杂志2012，33，2： | 8 |
| 7 | Interaction of major genes predisposing to hepatocellular carcinoma with genes encoding signal transduction pathways influences tumor phenotype and prognosis显示文摘啮齿类动物和人上的研究表明继承倾向到肝细胞癌(HCC ) 。涉及对 hepatocarcinogenesis 抵抗或易受影响的显型的获得的分子的改变的分析证明在遗传上易受影响的 F344 老鼠和 G1-S 的 HCC 的 G1 和 S 阶段的解除管制在抵抗布朗也不方法(BN ) 的损害堵住老鼠。连接到双特性的磷酸酶的 proteasomal 降级的无限制的细胞外的调整信号的激酶(英皇家空军之阶级最低之兵) 活动 1 (DUSP1 ) ,一个特定的英皇家空军之阶级最低之兵禁止者,由 CKS1-SKP2 ubiquitin ligase,建筑群发生在 F344 老鼠和人的更好攻击的 HCC。这机制与更好的预后在 BN 老鼠的 HCC 和人的 HCC 是不太活跃的。有 IKK/NF-kappaB 和地岬 / 英皇家空军之阶级最低之兵小径的 i Nos 串音的 Upregulation 在 F344 老鼠和 c-Myc-TGF-alpha 的 HCC 代表的最好攻击的模型在高水平发生在啮齿类动物肝损害转基因的老鼠。在规定上面的 i NOS, IKK/NF-kappaB,和地岬 / 英皇家空军之阶级最低之兵在有更差的预后的人的 HCC 是最高级的并且断然与肿瘤增长, genomic 不稳定性和 microvascularization 相关,并且否定地与 apoptosis。因此,房间周期规定和信号转导变异小径的活动似乎被 HCC 修饰词基因调制,并且在他们的效率的差别影响危险性到 hepatocarcinogenesis 并且可能人的 HCC 的预后。 | Francesco Feo Maddalena Frau Rosa Maria Pascale | 2008 | World Journal of Gastroenterology2008，14，43： | 5 |
| 8 | Preventive effect of regional radiotherapy with phosphorus-32 glass microspheres in hepatocellular carcinoma recurrence after hepatectomy显示文摘AIM: To evaluate the preventive effects of phosph-orus-32 glass microspheres (P32-GMS) in the recurrence of massive hepatocellular carcinomas (HCCs) after tumor resection. METHODS: Twenty-nine patients with massive HCCs received local P32-GMS implantation after liver tumors were removed,while the other 38 patients with massive HCCs were not treated with P32-GMS after hepatectomies. The radioactivity of the blood,urine and liver were examined. The complications,HCC recurrence and overall survival rates in the patients were analyzed. RESULTS: P32-GMS implanted in the liver did not cause systemic absorption of P32. There were no significant differences of postoperative complications between the patients with and without P32-GMS treatment. The short-term (six months and 1 year) and long-term (2,3 and over 3 years) recurrence rates in patients who received P32-GMS radiotherapy were signifi cantly decreased,and the overall survival rates in this group were signifi cantly improved. CONCLUSION: P32-GMS implantation in the liver can significantly decrease the postoperative recurrence and improve the overall survival in HCCs patients after hepatectomy. This therapy may provide an innovative method in prevention of HCC recurrence after operation. | Xiao-Ming Wang Zhen-Yu Yin Ren-Xiang Yu You-Yuan Peng Ping-Guo Liu Guo-Yang Wu | 2008 | World Journal of Gastroenterology2008，14，4： | 4 |
| 9 | 双靶向整合素αvβ3及VEGFR2微泡造影剂的制备及其对肝癌细胞寻靶能力的分析显示文摘本研究采用生物素亲和素法将整合素αvβ3及VEGFR2抗体与Usphere微泡结合,制备双靶向整合素αvβ3及VEGFR2微泡造影剂。制备成功的双靶向整合素αvβ3及VEGFR2微泡分布均匀,平均粒径(1205.06±103.70)nm,电位(-27.0±3.0)mV。将微泡与MHCC-97H肝癌细胞共孵育,免疫荧光法检测显示双靶向微泡组微泡荧光与细胞核DAPI荧光强度比值显著高于单靶向微泡组和非靶向微泡组(P<0.05)。提示本研究成功制备双靶向整合素αvβ3及VEGFR2微泡造影剂,可与MHCC-97H细胞特异性结合,可作为一种监测肿瘤生长的分子影像探针。 | 袁海霞 王莹 关佩珊 吴爱琴 金赟杰 王文平 | 2022 | 中华普通外科杂志2022，37，5： | 2 |
| 10 | p16基因的失活与肝癌生物学行为的关系显示文摘 | 周梅芳 沈明明 杨健 林彬 朱杰敏 冯放 | 2006 | 江苏医药2006，32，5： | 2 |
| 11 | Murine double minute 2, a potential p53-independent regulator of liver cancer metastasis显示文摘Hepatocellular carcinoma(HCC)has emerged as one of the most commonly diagnosed forms of human cancer;yet,the mechanisms underlying HCC progression remain unclear.Unlike other cancers,systematic chemotherapy is not effective for HCC patients,while surgical resection and liver transplantation are the most viable treatment options.Thus,identifying factors or pathways that suppress HCC progression would be crucial for advancing treatment strategies for HCC.The murine double minute 2(MDM2)-p53 pathway is impaired in most of the cancer types,including HCC,and MDM2 is overexpressed in approximately 30%of HCC.Overexpression of MDM2 is reported to be well correlated with metastasis,drug resistance,and poor prognosis of multiple cancer types,including HCC.Importantly,these correlations are observed even when p53 is mutated.Indeed,p53-independent functions of overexpressed MDM2 in cancer progression have been suitably demonstrated.In this review article,we summarize potential effectors of MDM2 that promote or suppress cancer metastasis and specifically discuss the p53-independent roles of MDM2 in liver cancer metastasis from clinical as well as biological perspectives. | Atul Ranjan Kaustav Bera Tomoo Iwakuma | 2016 | Hepatoma Research2016，2，1： | 2 |
| 12 | Identification of Max binding protein as a novel binding protein of Nckl and characterization of its role in inhibiting human liver cancer SK-HEP-1 cells显示文摘 | ZHOU Qi HUANG Tao WANG Ya-feng ZHANG Kun-sun CHEN Dong PENG Bao-gang | 2012 | Chinese Medical Journal2012，，18： | 2 |
| 13 | 老年肝癌的诊断与治疗显示文摘 | 周信达 | 2002 | 老年医学与保健2002，8，2： | 1 |
| 14 | 中耳胆脂瘤上皮过度增殖与MDM2的相关性显示文摘目的探讨MDM2(murinedoubleminute)在中耳胆脂瘤上皮增殖机制中的作用,了解胆脂瘤的过度增殖性和生长方式。方法应用免疫组织化学技术检测32例中耳胆脂瘤组和10例正常对照组中MDM2的表达。结果MDM2分布于胆脂瘤上皮全层,但在基底层细胞表达最弱,棘细胞层、颗粒层和角质层依次递增,染色最强的部位是颗粒层和角质层,而正常对照组未见MDM2分布。结论p53/MDM2反馈环异常可能是胆脂瘤上皮异常增殖和凋亡的原因之一。在胆脂瘤基底层细胞表达少量的MDM2可能是维持胆脂瘤不同于恶性肿瘤组织的无限增殖和浸润的基础。 | 汪欣 祝威 王苹 周志华 郭晓峰 | 2005 | 中国耳鼻咽喉头颈外科2005，12，10： | 1 |
| 15 | Arsenic trioxide inhibits metastatic potential of mouse hepatoma H22 cells in vitro and in vivo显示文摘BACKGROUND:It has been pointed out that only low-dose arsenic trioxide(ATO)presents therapeutic benefits outweighing the toxic side effects.Low-dose ATO can effectively alleviate acute promyelocytic leukemia(APL). However,it is quite challenging in treating solid tumors. The purpose of this study was to investigate the effect of ATO at low concentrations on the metastatic potential of mouse hepatoma H22 cells and the anti-metastatic mechanism of ATO. METHODS:The metastatic potential of H22 cells was evaluated by adhesion,migration and invasion assays after exposure to a low dose of ATO in vitro.The mouse lung metastatic model induced by injection of H22 cells via the tail vein was adopted for the evaluation of metastatic potential. Different proteins in the lysate of H22 cells exposed to ATO at different concentrations were investigated by surface- enhanced laser desorption and ionization time-of-flight mass spectrometry(SELDI-TOF-MS).Finally,Western blotting analyses were made to detect the expression pattern of MMP-2 and nm23-M1 proteins. RESULTS:Significant cell death started at ATO concentrations above 2μmol/L.The growth and adhesion potential of H22 cells was inhibited in a time-and dose- dependent manner,and the migration and invasion potential of H22 cells was inhibited in a dose-dependent manner while ATO concentration was below 2μmol/L. Mice injected with ATO at a dose of 0.5 mg/kg had fewer lung metastases.However,mice injected with ATO at a dose of 2 mg/kg or 4 mg/kg had a high mortality rate and more liver injuries.A total of 15 different protein peaks were identified between the lysate of H22 cells treated with ATO and controls.Two proteins that peaked atm/z 5302 and 17207 coincided with MMP-2(fragment) and nm23-M1,respectively.Western blotting analyses demonstrated that MMP-2 and MMP-2 fragments were down-regulated and nm23-M1 was up-regulated in H22 cells treated with 2μmol/L ATO for 48 hours. CONCLUSIONS:ATO at a low dose inhibits the metastatic potential of mouse hepatoma H22 cells in vitro and in vivo, and involves down-regulation of MMP-2 and up-regulation of nm23-M1. | | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，5： | 1 |
| 16 | 预防性介入在肝癌术后治疗中的价值显示文摘目的评价预防性介入在肝癌患者术后治疗中的价值。方法收集2010年我院肝癌术后患者73例进行回顾性分析。A组32例患者于肝癌术后行预防性介入治疗,B组41例患者未行预防性介入治疗。比较A、B两组患者1、2、3、4年复发率及生存率的差异。结果两组患者1、2、3、4复发率的差异无统计学意义;两组患者1、2,3年生存率差异有统计学意义,两组患者4年生存率差异无统计学意义。结论预防性介入并未降低肝癌患者术后复发率,但可能提高1-3年近期生存率。 | 罗荣光 田秋红 钟久鸿 张战民 曾兵文 | 2015 | 江西医药2015，50，11： | 0 |
| 17 | Hypoxia-induced enhancement of cell invasiveness in SMMC7721 hepatocellular carcinoma cells显示文摘Objective To explore the effects of hypoxia(1% O2)on the ability of cell invasiveness and expression of KAI1/CD82 in SMMC7721 hepatocellular carcinoma cells.Methods SMMC7721 hepatocellular carcinoma cells were cultured by hypoxia(1% O2)in vitro,and the ability of cell invasiveness was analyzed by cell invasion assay.Immunohistochemistry staining technique was used to evaluate the protein expression of KAI1/CD82.Results Cell invasion assay revealed that hypoxia enhanced the ability of invasiveness of hepatocellular carcinoma cells.In addition,KAI1/CD82 protein expression was positive in cultured SMMC7721 hepatocellular carcinoma cells,and it was located diffusedly in the cytoplasm and on the membrane.KAI1/CD82 protein expression was down-regulated when mediated by hypoxia;at the same time,it showed a time-effect relationship.Conclusion Hypoxia can enhance invasiveness of hepatocellular carcinoma cells.The down-regulation of KAI1/CD82 expression may play a certain role in those courses. | Xue Yang,Cheng Guo,Lei Zhang,Xin Zheng,Wei Yang,Qing-guang LiuDepartment of Hepatobiliary Surgery,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061,China | 2009 | Journal of Pharmaceutical Analysis2009，21，4： | 0 |
| 18 | 原发性肝细胞肝癌组织中IMP3表达与临床特点和基因甲基化相关性的研究显示文摘目的:研究IMP3在原发性肝细胞肝癌中的表达意义以及基因甲基化同IMP3表达的关系。方法:免疫组化检测162例肝细胞肝癌组织中IMP3的表达。分析临床因素与IMP3表达水平的相关性,并随访所选162例术后患者生存情况作生存分析。提取正常肝组织、肝癌组织的DNA,通过甲基化特异性定量PCR测定IMP3启动子CpG岛甲基化状态。培养正常肝脏细胞L02和Huh-7肝癌细胞,通过Western blot检测IMP3蛋白的表达。提取两组细胞DNA,分别对IMP3基因启动子区域CpG岛进行甲基化状态检测。结果:原发性肝细胞肝癌组织中IMP3阳性率为67.90%;IMP3表达与患者年龄、肿瘤大小、分级、分期、AFP、早期复发均存在相关性;而与性别、HBsAg无明显相关性。随访显示IMP3阳性患者长期生存明显低于IMP3阴性者。CpG岛甲基化检测显示肝癌组织中P1和P2位点高度去甲基化,而正常肝脏组织中P1和P2则处于甲基化状态。正常肝脏细胞IMP3表达阴性,Huh-7肝癌细胞IMP3表达强阳性。正常肝细胞P1和P2位点处于高度甲基化状态,而Huh-7肝癌细胞P1和P2位点处于去甲基化状态。结论:IMP3在原发性肝细胞肝癌中具有高表达,IMP3高表达提示不良预后;基因的甲基化同IMP3的表达存在一定的相关性。 | 黄新立 高园园 丁军 王学浩 | 2014 | 南京医科大学学报(自然科学版)2014，34，5： | 0 |
| 19 | 内皮素-1和细胞间黏附分子-1检测在胸腹水鉴别诊断中的价值显示文摘目的 通过检测不同病因胸腹水中内皮素-1(ET-1)和细胞间黏附分子1(ICAM-1)水平,探讨其在鉴别胸腹水性质中的意义。方法 83例来自临床上已确诊病人的胸腹水标本,分为三组:A.结核组(21例);B.恶性肿瘤组(29例);C.肝硬化组(33例)。采用放免法检测ET-1含量,酶联免疫吸附法(ELISA)测ICAM-1水平。结果 结核性腹水ICAM-1水平为129.67±39.72ng/L(x±s),肿瘤性腹水水平为72.63±32.75ng/L,肝硬化腹水水平为30.55±19.88 ng/L,结核性腹水ET-1水平为68.24±19.48 ng/L,肿瘤性腹水水平为42.17±16.40 ng/L,肝硬化腹水水平为22.46±16.77 ng/L,ICAM-1和ET-1水平在结核性腹水中明显高于肿瘤性腹水(P<0.05)和肝硬化性腹水(P<0.01),两者水平在肿瘤性腹水和肝硬化腹水之间的也存在显著性差异(P<0.05)。结论ICAM-1和ET-1的表达与机体炎症反应被激活及肿瘤的侵袭与转移有关,故结核性及肿瘤性胸腹水中ICAM-1和ET-1水平有不同程度的增高,ET-1及ICAM-1水平可作为一种筛选手段,以初步鉴别胸腹水的性质。 | 孙军 尼克赫.阿里 余保平 罗和生 沈志祥 | 2004 | 胃肠病学和肝病学杂志2004，13，3： | 0 |
| 20 | 肝胆胰恶性肿瘤的生物治疗显示文摘 | 温浩 朱功兵 王喜艳 | 2005 | 腹部外科2005，18，2： | 0 |
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