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| 1 | 集胞藻Slr1515基因提高转基因水稻植株剑叶长度及叶绿素含量显示文摘蓝藻是古老的原核光合生物,不仅在植物进化上有重要地位,也是可利用的生物资源及重要的基因资源。Slr1515是来源于集胞藻6803(Synechocystis sp.PCC 6803)的基因,其同源基因在蓝藻中普遍存在,但对其生物学功能所知有限。以易于转化的水稻品种中花11(Oryza sativa subsp.japonica cv.‘Zhonghua 11’)为材料,通过农杆菌介导的遗传转化方法将Slr1515基因导入水稻胚性愈伤组织中。经过诱导分化,获得了一系列转基因幼苗。通过PCR、GFP荧光及免疫印迹检测,筛选出单拷贝插入的转基因阳性株系,并对野生型和转基因株系的表型进行比较分析。结果表明,灌浆期转基因株系的剑叶长度比同期的野生型提高了27.7%。进一步的测定结果显示,转基因株系的剑叶叶绿素含量和PSII最大量子产率分别比野生型提高了0.65倍和4.4%。推测Slr1515基因可能在改善水稻农艺性状方面有一定价值。 | 杨浩萌 薛哲勇 赵乐 漆小泉 黄芳 | 2013 | 植物学报2013，48，5： | 2 |
| 2 | Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress显示文摘The genome of unicellular green alga Chlamydomonas reinhardtii contains four genes encoding B-type methionine sulfoxide reductases,MSRB1.1,MSRB1.2,MSRB2.1,and MSRB2.2,with functions largely unknown.To understand the cell defense system mediated by the methionine sulfoxide reductases in Chlamydomonas,we analyzed expression and physiological roles of the MSRBs under different abiotic stress conditions using immunoblotting and quantitative polymerase chain reaction(PCR)analyses.We showed that the MSRB2.2 protein was accumulated in cells treated with high light(1,300 mE/m2per s),whereas MSRB1.1 was accumulated in the cells under 1 mmol/L H2O2treatment or sulfur depletion.We observed that the cells with the MSRB2.2 knockdown and overexpression displayed increased and decreased sensitivity to high light,respectively,based on in situ chlorophyll a fuorescence measures.We also observed that the cells with the MSRB1.1 knockdown and overexpression displayed decreased and increased tolerance to sulfur-depletion and oxidative stresses,respectively,based on growth and H2-producing performance.The physiological implications revealed from the experimental data highlight the importance of MSRB2.2 and MSRB1.1 in protecting Chlamydomonas cells against adverse conditions such as high-light,sulfur-depletion,and oxidative stresses. | Lei Zhao Mei Chen Dongmei Cheng Haomeng Yang Yongle Sun Heyi Zhou Fang Huang | 2013 | Journal of Integrative Plant Biology2013，55，11： | 2 |
| 3 | 集胞藻sbtA基因具有提高水稻旗叶叶绿素含量及株高的功能显示文摘SbtA是蓝藻特有的碳酸氢盐转运蛋白,在蓝藻二氧化碳浓缩机制中起重要作用。本研究以水稻‘中花11’为材料,采用农杆菌介导的转化方法将集胞藻的sbtA基因导入水稻胚性愈伤组织中,再经诱导分化获得转基因幼苗。通过PCR、GFP荧光检测及Western blot检测筛选单拷贝插入的转基因阳性株系,并对其表型进行比较分析。结果表明,转基因水稻株高和旗叶的叶绿素含量显著提高,与野生型相比分别增加了11.9%和27%。后一表型暗示sbtA基因可能在延缓灌浆期水稻旗叶衰老及改善水稻农艺性状方面具有潜在的应用价值。 | 杨浩萌 薛哲勇 赵乐 漆小泉 黄芳 | 2013 | 植物生理学报2013，49，4： | 2 |
| 4 | Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms:relevance to excitotoxic neurodegeneration显示文摘Background:Glutaminase 1 is a phosphate-activated metabolic enzyme that catalyzes the first step of glutaminolysis,which converts glutamine into glutamate.Glutamate is the major neurotransmitter of excitatory synapses,executing important physiological functions in the central nervous system.There are two isoforms of glutaminase 1,KGA and GAC,both of which are generated through alternative splicing from the same gene.KGA and GAC both transcribe 1–14 exons in the N-terminal,but each has its unique C-terminal in the coding sequence.We have previously identified that KGA and GAC are differentially regulated during inflammatory stimulation and HIV infection.Furthermore,glutaminase 1 has been linked to brain diseases such as amyotrophic lateral sclerosis,Alzheimer’s disease,and hepatic encephalopathy.Core enzyme structure of KGA and GAC has been published recently.However,how other coding sequences affect their functional enzyme activity remains unclear.Methods:We cloned and performed serial deletions of human full-length KGA and GAC from the N-terminal and the C-terminal at an interval of approximately 100 amino acids(AAs).Prokaryotic expressions of the mutant glutaminase 1 protein and a glutaminase enzyme activity assay were used to determine if KGA and GAC have similar efficiency and efficacy to convert glutamine into glutamate.Results:When 110 AAs or 218 AAs were deleted from the N-terminal or when the unique portions of KGA and GAC that are beyond the 550 AA were deleted from the C-terminal,KGA and GAC retained enzyme activity comparable to the full length proteins.In contrast,deletion of 310 AAs or more from N-terminal or deletion of 450 AAs or more from C-terminal resulted in complete loss of enzyme activity for KGA/GAC.Consistently,when both Nand C-terminal of the KGA and GAC were removed,creating a truncated protein that expressed the central 219 AA-550 AA,the protein retained enzyme activity.Furthermore,expression of the core 219 AA-550 AA coding sequence in cells increased extracellular glutamate concentrations to levels comparable to those of full-length KGA and GAC expressions,suggesting that the core enzyme activity of the protein lies within the central 219 AA-550 AA.Full-length KGA and GAC retained enzyme activities when kept at 4°C.In contrast,219 AA-550 AA truncated protein lost glutaminase activities more readily compared with full-length KGA and GAC,suggesting that the Nterminal and C-terminal coding regions are required for the stability KGA and GAC.Conclusions:Glutaminase isoforms KGA and GAC have similar efficacy to catalyze the conversion of glutamine to glutamate.The core enzyme activity of glutaminase 1 protein is within the central 219 AA-550 AA.The N-terminal and C-terminal coding regions of KGA and GAC help maintain the long-term activities of the enzymes. | Yuju Li Justin Peer Runze Zhao Yinghua Xu Beiqing Wu Yi Wang Changhai Tian Yunlong Huang Jialin Zheng | 2017 | Translational Neurodegeneration2017，6，1： | 1 |
| 5 | Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem Ⅱ under high-light condition显示文摘Cyanobacteria are ancient photosynthetic prokareyotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem Ⅱ(PSⅡ) in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited.We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp.PCC 6803. Here, we report that Slr0151 is involved in PSⅡ repair of the organism. We generated a mutant strain(Dslr0151)lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSⅡ activity in Synechocystis. Moreover,the mutant exhibited increased photoinhibition due to impairment of PSⅡ repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSⅡ repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSⅡ in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together,these results indicate that Slr0151 plays an important role in regulating PSⅡ repair in the organism under high-light stress condition. | Haomeng Yang Libing Liao Tingting Bo Lei Zhao Xuwu Sun Xuefeng Lu Birgitta Norling Fang Huang | 2014 | Journal of Integrative Plant Biology2014，56，12： | 1 |
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