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| 1 | 苹果磷酸果糖激酶基因MdPFPβ调控果实可溶性糖积累的功能显示文摘为阐明苹果(Malus×domestica Borkh.)磷酸果糖激酶基因MdPFPβ(Pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit beta,基因序列号MD09G1112800)的生物学功能,首先分析了基因启动子和全长序列信息,该基因包含全长为483 bp完整的开放阅读框,编码161个氨基酸。利用PlantCare数据库进行基因启动子顺式作用元件分析发现,MdPFPβ启动子序列中含有与脱落酸(abscisicacid,ABA)、低温及干旱信号相关的调控元件。荧光定量PCR分析发现,MdPFPβ在苹果叶片和成熟果实中表达量较高,并且受外源ABA的诱导表达。在外施ABA的条件下,MdPFPβ过量表达的苹果愈伤组织中可溶性糖含量明显上升。此外,MdPFPβ转基因番茄植株的光合性能增强,MdPFPβ可能通过调控糖代谢酶的活性最终影响果实中的可溶性糖含量。 | 于建强 顾凯迪 王传增 胡大刚 | 2022 | 园艺学报2022，49，10： | 1 |
| 2 | 植物中C-糖基转移酶的结构与应用显示文摘糖基转移酶是一类专门催化糖基化反应的后修饰酶,根据与糖供体连接的受体分子不同,分为O-、C-、N-、S-四种糖基转移酶。近年来,伴随着结构生物学的不断发展,关于C-糖基转移酶的结构解析及定向改造的报道日益增多,为复杂天然产物修饰提供理论依据。本文综述了植物C-糖基转移酶的研究进展,从其底物杂泛性、糖供体结合的空间结构与生物技术应用的角度进行讨论,探究造成底物催化特征的结构基础,为后续通过合成生物学手段异源合成植物天然产物提供参考。 | 赵玉雪 王芸 余璐瑶 刘京晶 斯金平 张新凤 张磊 | 2022 | 生物技术通报2022，38，10： | 0 |
| 3 | Identification of UGT85A glycosyltransferases associated with volatile conjugation in grapevine(Vitis vinifera × Vitis labrusca)显示文摘Glycosylated volatiles are considered as hidden aroma precursors that affect fruit flavor and are involved in plant defense response. Several uridine-diphosphate(UDP)-glycosyltransferase(UGT) members related to the glycosylation of volatiles have been identified from grapevine berries. Although grapevine leaves produced much higher levels of glycosylated volatiles than berries, UGTs responsible for the production of glycosylated volatiles in leaves have not yet been identified. Here, eight novel UGT genes were cloned, among these four members that belong to UGT85A family(named UGT85A24, UGT85A25, UGT85A26, and UGT85A27) were localized in the cytoplasm and their recombinant proteins showed activity toward volatiles detected in grapevine, with geraniol as the preferred substrate in vitro. Two UGT genes with 98.1% homology differ in R/S-linalool selection. The UGT85A26-catalyzed substrate showed enrichment in S-linalool, whereas UGT85A27 showed a strong preference for R-linalool. UGT85A24 and UGT85A25 were expressed mainly in berries, and UGT85A26 and UGT85A27 were expressed predominantly in grapevine mature leaves and correlated with the highest content of glycosylated volatiles in the mature leaves. Methyl jasmonate(MeJA) induced the expression of UGT85A26 and UGT85A27 as well as the production of linalyl-β-glucoside, citronellyl-β-glucoside and phenethyl-β-glucoside. Transient overexpression of UGT85A26 and UGT85A27 in tobacco leaves led to the accumulation of linalyl-β-glucoside and an increase in citronellyl-β-glucoside in vivo. These results indicate that the expression of UGT85A26 and UGT85A27 is a major regulator affecting the content of glycosylated volatiles in grapevine. | Xiangmei Cao Dan Jiang Hanqing Wang Boping Wu Jianhui Cheng Bo Zhang | 2023 | Horticultural Plant Journal2023，9，6： | 0 |
| 4 | Comprehensive analyses of the proteome and ubiquitome revealed mechanism of high temperature accelerating petal abscission in tree peony显示文摘Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony. | Chunying Liu Ziqi Liu Yanchao Yuan Yuxi Zhang Yifu Fang Junqiang Chen Shupeng Gai | 2024 | Horticultural Plant Journal2024，10，1： | 0 |
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