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| 1 | 蒺藜苜蓿生物钟基因MtTOC1a的克隆及功能验证显示文摘【目的】分析蒺藜苜蓿Medicago truncatula生物钟基因MtTOC1a的蛋白结构,探究MtTOC1a在生物钟系统中的生物学功能,比较其与拟南芥Arabidopsis thaliana的AtTOC1功能相似性和差异性。【方法】通过生物信息学分析,在全基因组范围内鉴定了TOC1在蒺藜苜蓿中的同源基因。构建MtTOC1a基因的表达载体,利用农杆菌介导法引入到拟南芥野生型Col、及相应的功能丧失突变体toc1-2中,进行遗传互补分析。【结果】MtTOC1a和MtTOC1b均具有保守的功能结构域和三维结构。遗传分析表明,在早期光形态建成中,外源转化的MtTOC1a完全恢复了toc1-2的下胚轴伸长表型,但对toc1-2的提前开花表型没有显著影响。在引入CAB::LUC报告基因的株系中,外源转化MtTOC1a在连续光照下使短周期突变体toc1-2的近日节律周期延长,但仍不能完全恢复至野生型水平。【结论】MtTOC1a和拟南芥AtTOC1的功能存在相似性,但在不同的下游调控途径中所扮演的角色存在差异。本研究结果为进一步探索MtTOC1a基因的功能,利用MtTOC1a基因改造苜蓿的重要性状提供了理论依据。 | 段婷婷 杨明康 黄可 黄巍 | 2023 | 华南农业大学学报2023，44，5： | 0 |
| 2 | Flowering-time regulation by the circadian clock:From Arabidopsis to crops显示文摘Precise timing of flowering in plants is critical for their growth and reproductive processes.One factor controlling flowering time is the cycle of light and darkness within a day,known as the photoperiod.Plants are classified into long-day,short-day,and day-neutral plants based on light requirements for floral initiation.Although the molecular mechanisms that govern this differentiation remain incompletely understood,studies have consistently shown that the circadian clock plays a central role in regulating photoperiod response across diverse plant species.However,there is a scarcity of reviews describing the regulatory network linking the circadian clock with photoperiodic flowering.This review summarizes that regulatory network,focusing on the distinct roles of clock genes in long-day and short-day plants.We also discuss the strategies of clock gene mutations contributing to geographic variation in longday and short-day crops. | Mingkang Yang Wenjie Lin Yarou Xu Biyu Xie Baiyin Yu Liang Chen Wei Huang | 2024 | The Crop Journal2024，12，1： | 0 |
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