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| 1 | Towards Understanding of Molecular Interactions between Rice and the Brown Planthopper显示文摘棕色的 planthopper (BPH ) 是米饭(Oryza sativa ) 的最臭名昭著的害虫。riceBPH 相互作用的研究贡献了新米饭变化的开发,提供为 BPH 的长持续的控制的一个有效工具。这里,我们考察 riceBPH 相互作用的分子的基础的知识的地位,从免疫的观点。BPH 在米饭上有复杂喂的行为,它主要与主人抵抗有关。现在, 24 抵抗基因在米饭被检测了,显示与 BPH 的遗传因子型的 gene-for-gene 关系。然而,一仅仅 BPH 抵抗基因(Bph14 ) 被识别并且描绘了使用基于地图的克隆。Bph14 编码 NBLRR 家庭的有免疫力的受体,提供为学习到 BPH 的米饭抵抗的分子的机制的一个工具。植物荷尔蒙(例如水杨酸酸和 jasmonate/ethylene ) , Ca2+ ,激活 mitogen 的蛋白质 kinases (MAPK ) ,和 OsRac1 在对 BPH 的米饭的有免疫力的反应起重要作用。信号 transduction 导致对 BPH 修改防卫相关的基因和防卫机制的表示,包括筛试管封上,第二等的代谢物的生产,和朊酶禁止者的正式就职。为在米饭和 BPH 之间的分子的相互作用的一个模型被建议,尽管许多细节尚待被调查那为 BPH 抵抗的米饭变化的分子的设计是珍贵的。 | Xiaoyan Cheng Lili Zhu Guangcun He | 2013 | Molecular Plant2013，6，3： | 33 |
| 2 | 水稻抗褐飞虱基因的研究显示文摘评述了水稻抗褐飞虱基因研究的进展。褐飞虱在热带越冬 ,每年 4~ 8月逐代北迁 ,是我国分布最广、为害最重的稻作害虫。褐飞虱栖息于稻丛基部 ,以刺吸式口器吸食韧皮部汁液 ,严重时稻田成片枯萎 ,造成减产甚至失收。褐飞虱也是一些水稻病毒病的传播媒介。在水稻抗褐飞虱基因研究中通常采用人工接虫方法鉴定材料的抗虫性 ,以达到大批量、指标量化和可重复的要求。常用的方法有苗期集团鉴定、虫口密度及产卵量测定、蜜露量测定等。褐飞虱种群中有生物型的变异。在抗虫品种抗性的选择压力下 ,褐飞虱群体致害性会发生变化 ,经多次传代后可转变为能致害该抗虫品种的新的生物型。水稻抗褐飞虱基因的研究始于 2 0世纪 70年代初 ,至今已命名了 10多个主效基因 ,并鉴定了重要的QTL。通过分子标记分析 ,一些抗褐飞虱基因已被定位。克隆水稻的抗褐飞虱基因是下一阶段研究的关键课题。 | 祝莉莉 祝彩磊 翁清妹 黄臻 何光存 | 2004 | 湖北农业科学2004，43，1： | 13 |
| 3 | 水稻抗稻飞虱基因遗传与定位研究进展显示文摘褐飞虱(Nilaparvata lugensStal)、白背飞虱(Sogatella furciferaHorvath)和灰飞虱(Laodelphax striatellusFaléln)是水稻生产上的重要害虫,给我国水稻生产造成了严重的经济损失。培育和利用抗虫品种是防治稻飞虱经济有效的措施。抗性遗传和抗性基因研究是进行抗虫育种的基础。目前,有关水稻抗褐飞虱基因的遗传与定位研究取得了较大进展,包括21个主基因、50余个QTLs和许多褐飞虱抗性相关基因被发掘、定位与克隆,而白背飞虱和灰飞虱抗性基因尚有待进一步发掘和鉴定。此外,今后应加强稻飞虱抗性基因在生产上的应用。 | 余娇娇 段灿星 李万昌 朱振东 王晓鸣 | 2011 | 植物遗传资源学报2011，12，5： | 9 |
| 4 | Bph30confers resistance to brown planthopperby fortifying sclerenchyma in rice leaf sheaths显示文摘Phloem-feeding insects cause massive losses in agriculture and horticulture.Host plant resistance to phloem-feeding insects is often mediated by changes in phloem composition,which deter insect settling and feeding and decrease viability.Here,we report that rice plant resistance to the phloem-feeding brown planthopper(BPH)is associated with fortification of the sclerenchyma tissue,which is located just beneath the epidermis and a cell layer or two away from the vascular bundle in the rice leaf sheath.We found that BPHs prefer to feed on the smooth and soft region on the surface of rice leaf sheaths called the long-cell block.We identified Bph30 as a rice BPH resistance gene that prevents BPH stylets from reaching the phloem due to the fortified sclerenchyma.Bph30 is strongly expressed in sclerenchyma cells and enhances cellulose and hemicellulose synthesis,making the cell walls stiffer and sclerenchyma thicker.The structurally fortified sclerenchyma is a formidable barrier preventing BPH stylets from penetrating the leaf sheath tissues and arriving at the phloem to feed.Bph30 belongs to a novel gene family,encoding a protein with two leucine-rich domains.Another member of the family,Bph40,also conferred resistance to BPH.Collectively,the fortified sclerenchyma-mediated resistance mechanism revealed in this study expands our understanding of plant-insect interactions and opens a new path for controlling planthoppers in rice. | Shaojie Shi Huiying Wang Lingyun Nie Di Tan Cong Zhou Qian Zhang YiLi Bo Du Jianping Guo Jin Huang DiWu Xiaohong Zheng Wei Guan Junhan Shan Lili Zhu Rongzhi Chen Longjian Xue Linda L.Walling Guangcun He | 2021 | Molecular Plant2021，14，10： | 7 |
| 5 | Bowman-Brik胰蛋白酶抑制剂在水稻叶片生长和种子萌发过程中的表达显示文摘Bowman-Birk胰蛋白酶抑制剂(BBTI)是一类富含半胱氨酸的丝氨酸蛋白酶抑制剂,广泛分布于豆科、禾本科和菊科植物中,在蛋白质存储和植物防御中具有重要作用。水稻(Oryza sativa L.ssp.indica)BBTI家族具有13个成员,其中BBTI-9和BBTI-10都为假基因。其余BBTI成员都存在数量不同的保守Bowman-Birk结构域。为了解BBTI的表达信息,本研究主要通过设计多肽序列的方法,制备了11个BBTI的多克隆抗体,利用Western blot技术检测其在叶片生长发育和种子萌发过程中的表达。结果表明BBTI在叶片生长过程中呈现不同的时空特异性表达,BBTI-1、-2、-11、-12和-13伴随叶片生长含量增加,BBTI-5、-6和-8在苗期表达含量高,而BBTI-3、-4、-7在苗早期1cm时表达量达到峰值,以修饰或多聚体的形式在叶片生长过程中表达。在种子萌发过程中发现BBTI-8在种子中表达量较高,萌发后2h降到最低,24h后一条分子量略小的降解带开始表达,其余抗体在种子萌发阶段未发现有明显的变化。本研究还统计了苗期叶片、分蘖期叶片和萌发期的种子的MPSStags数据,试图比较转录与蛋白质水平对应关系。本研究结果证明,BBTI蛋白质在水稻生长发育过程中存在差异表达,为深入了解其功能提供了重要线索。 | 徐文静 李莉云 史佳楠 李静宜 巩校东 刘丽娟 刘国振 | 2012 | 农业生物技术学报2012，20，3： | 4 |
| 6 | Expression Analysis of Genes Related to Rice Resistance Against Brown Planthopper, Nilaparvata lugens显示文摘Brown planthopper(BPH) is an insect species that feeds on the vascular system of rice plants. To examine the defence mechanism of rice plants against BPH, the pathogenesis-related genes(PR1a, PR2, PR3, PR4, PR6, PR9, PR10a, PR13, PR15 and PRpha), signaling molecule synthesis genes(AOS, AXR, ACO and LOX), antioxidant-related genes(CAT, TRX, GST and SOD) and lignin biosynthesis-related genes(CHS, CHI and C4H) were investigated in a resistant rice variety. AOS, PR6,PR9 and PR15 genes showed significantly increased relative expression levels at 24.38-, 19.17-, 14.71-, and 12.74-fold compared to the control. Moderate increased relative expression levels of lignin biosynthesis-related gene(C4H), pathogenesis-related genes(PR4, PR10a and PRpha), and antioxidant-related gene(GST) were found, while CHI, LOX, SOD, TRX1 and AXR showed decreased relative expression levels. It was thus clearly shown that wound-induced response genes were activated in rice plants after BPH attacks through AOS activation. Jasmonic acid signaling molecule may activate PR6, PR15, GST and CAT subsequently increasing their expression for H_2O_2 detoxification. PR6 were expressed at the highest relative level among the PR genes. These genes therefore have also a considerable synergistic role with the other genes against BPH by interfered their digestion tract system. | Panatda JANNOEY Duangdao CHANNEI Jate KOTCHARERK Weerathep PONGPRASERT Mika NOMURA | 2017 | Rice science2017，24，3： | 4 |
| 7 | Localization, expression, and secretion pathway of diapause hormone in embryo and larva of the silkworm, Bombyx mori显示文摘In this paper, the distribution and expression of diapause hormone (DH) at mRNA and protein levels in the central nervous system of Bombyx mori embryo and larva were studied using whole-mount in situ hybridization and immunocytochemistry. Whole-mount immunocytochemistry revealed that the distribution of the DH-like immunoreactivity was throughout the central nervous system including the brain, suboesophageal ganglion (SG) and thoracic ganglia(TG); and that the corpus cardiacum and terminal abdominal ganglion may be the site for DH release due to the presence of strong immunoreactivity. In situ hybridization with the probe labeled by digoxigenin shows that the BomDHmRNA was also localized in the mandibular, maxillary, labial cell clusters. In addition, a pair of lateral neurons in the SG and a pair of ventral midline neurons in each TG expressing the Bom-DH transcript were also identified. Thesere sults were consistent with the localization of Bom-DH mRNA in larva by in situ hybridization and the distribution of the gene by RT-PCR, which is some different from the results reported previously. | SUNJiusong CHENFusheng XUWeihua | 2004 | Chinese Science Bulletin2004，49，13： | 3 |
| 8 | Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants显示文摘The brown planthopper(BPH)(Nilaparvata lugens St?l)is a highly destructive pest that seriously damages rice(Oryza sativa L.)and causes severe yield losses.To better understand the physiological and metabolic mechanisms through which BPHs respond to resistant rice,we combined mass-spectrometry-based lipidomics with transcriptomic analysis and gene knockdown techniques to compare the lipidomes of BPHs feeding on either of the two resistant(NIL-Bph6 and NIL-Bph9)plants or a wild-type,BPH susceptible(9311)plant.Insects that were fed on resistant rice transformed triglyceride(TG)to phosphatidylcholine(PC)and digalactosyldiacylglycerol(DGDG),with these lipid classes showing significant alterations in fatty acid composition.Moreover,the insects that were fed on resistant rice were characterized by prominent expression changes in genes involved in lipid metabolism processes.Knockdown of the NlBmm gene,which encodes a lipase that regulates the mobilization of lipid reserves,significantly increased TG content and feeding performance of BPHs on resistant plants relative to dsGFP-injected BPHs.Our study provides the first detailed description of lipid changes in BPHs fed on resistant and susceptible rice genotypes.Results from BPHs fed on resistant rice plants reveal that these insects can accelerate TG mobilization to provide energy for cell proliferation,body maintenance,growth and oviposition. | Xiaohong Zheng Yeyun Xin Yaxin Peng Junhan Shan Ning Zhang Di Wu Jianping Guo Jin Huang Wei Guan Shaojie Shi Cong Zhou Rongzhi Chen Bo Du Lili Zhu Fang Yang Xiqin Fu Longping Yuan Guangcun He | 2021 | Science China(Life Sciences)2021，64，9： | 1 |
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