|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection显示文摘AIM To identify the property of dendritic cella (DCs) of peripheral blood monocytes (PBMC) in patlents with chronic HBV infection.METHODS Twenty patients with persistent HBV infectlon were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GMCSF, IL-4, FLt-3, TNF-α and 100 mL@ L-1 of fetal calf serum for a total of 10 - 12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation,mytomycin C-treated DC were incubated with allogenic T cells.RESULTS A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLADR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0. 84 for CD86, P< 0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCa simultaneously increased compared with healthy subjects (86± 15 vs 170±22 μmoI@L 1, P<0.05).CONCLUSION The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV. | Fu-Sheng Wang Li-He Xing Ming-Xu Liu Chuan-Lin Zhu Hui-Gang Liu Hui-Fen Wang Zhou-Yun Lei Division of Biological Engineering,~2 Fourth Department of Liver Diseases,Beijing Institute of Infectious Diseases,Beijing Hospital of Infectious Diseases,Beijing 100039,China | 2001 | World Journal of Gastroenterology2001，7，4： | 131 |
| 2 | Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients显示文摘AIM: To analyze the correlation between cytokineinduced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients. METHODS: One hundred and fifty-six gastric cancer patients after operation at the Third Affiliated Hospital of Soochow University were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model. RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ 2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P < 0.05). The 2- and 5-year survival rates of gastric cancer patients were significantly higher after CIK cells adoptive immunotherapy than after chemotherapy (73.5% vs 52.6%, 40.4% vs 23.9%, P < 0.05). A significant difference was observed in the survival curve for patients who received CIK cells adoptive immunotherapy (0, 1-10, 11-25, and over 25 frequencies) (χ 2 = 14.534, P = 0.002). The frequencies of CIK cells adoptive immunotherapy were significantly related with the decreasing risk of death in gastric cancer patients after adjustment for sex and age of the patients, tumor stage and relapse (HR = 0.54, 95% CI: 0.36-0.80) when the first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of death in CIK cells adoptive immunotherapy and the risk of gastric cancer patients (HR = 1.09, 95% CI: 0.63-0.89) when the second stage Cox model was used to define the subjects who survived for more than 36 mo as survivors. CONCLUSION: The survival time of the gastric cancer patients treated with chemotherapy combined with CIK cells adoptive immunotherapy is significantly longer than that of the patients treated with chemotherapy alone and increasing the frequency of CIK cells adoptive immunotherapy seems to benefit patients more. | Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, Department of Tumor Biological Treatment, Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215123, Jiangsu Province, China Yue-Ping Shen, Wen-Xiang Wei, Department of Medicine, Soochow University, Suzhou 215123, Jiangsu Province, China Bin-Feng Lu, Department of Immunology, University of Pitts- burgh School of Medicine, Pittsburgh, PA 15261, United States | 2010 | World Journal of Gastroenterology2010，16，48： | 82 |
| 3 | 区域物流能力与区域经济发展的典型相关分析——基于全国面板数据显示文摘从物流基础设施、物流经济产出、物流产业规模等方面的指标体系入手,构建包含区域经济产出、社会物资需求、基础设施投资等方面的区域经济指标体系。基于2003~2008年中国31个省市的面板数据,利用典型相关分析方法对区域物流能力与区域经济发展进行实证分析。结果表明区域物流能力与区域经济发展高度相关,并结合其他统计分析结果数据提出了促进区域物流能力发展的建议。 | 李全喜 金凤花 孙磐石 | 2010 | 软科学2010，24，12： | 104 |
| 4 | 基于TRIZ理论和功能分析的产品创新设计显示文摘阐述了产品功能分析法、TRIZ理论的最终理想解和物场分析方法,建立了基于TRIZ理论和功能分析的产品创新设计流程。该流程将产品的创新设计分为产品功能分析、抽象理想解,构建系统物场模型和解决问题功能模型几个阶段,指导设计人员从产品的功能出发,快速实现产品的创新设计。并以液压缸往复密封为例,说明该流程的有效性。 | 卢希美 张付英 张青青 | 2010 | 机械设计与制造2010，，12： | 67 |
| 5 | 复杂嵌入式实时系统体系结构设计与分析语言:AADL显示文摘首先归纳了AADL(architecture analysis and design language)的发展历程及其主要建模元素.其次,从模型驱动设计与实现的角度综述了AADL在不同阶段的研究与应用,总结了研究热点,分析了现有研究的不足,并对AADL的建模与分析工具、应用实践进行了概述.最后,探讨了AADL的发展与研究方向. | 杨志斌 皮磊 胡凯 顾宗华 马殿富 | 2010 | 软件学报2010，21，5： | 75 |
| 6 | 不同产地野生黑果枸杞资源果实多酚组成分析显示文摘目的黑果枸杞(Lycium ruthenicum Murr.)主要分布于中国西北地区。其果实在传统中药中被用来治疗心脏病、月经异常及停经等疾病。近年来,发现多酚特别是花色苷类物质是其主要的功效成分。测定并评价不同产地野生黑果枸杞果实中花色苷等多酚的组成情况,为黑果枸杞的品种选育及开发利用提供依据。方法采用分光光度计测定其总酚、总黄酮、总花色苷含量;采用HPLC-MS方法分析鉴定黑果枸杞酚类物质组成及结构;并根据总多酚含量、总黄酮含量、总花色苷含量及多酚共有峰峰面积分别对26个不同产地黑果枸杞的多酚检测结果进行聚类分析。结果 26个不同产地的黑果枸杞资源果实的总酚含量、总黄酮含量、总花色苷含量的变化幅度分别为8.25—87.77 mg GAE·g-1、18.03—60.44 mg RE·g-1、8.21—31.46 mg·g-1。不同产地的材料间差异较大,其中青海格尔木乌图美仁、新疆甘河子、新疆巴仑台镇、宁夏贺兰12-01黑果枸杞总酚、总黄酮及总花色苷含量较高;通过HPLC-MS分析鉴定,19个多酚类化合物被鉴定出。其中7个为酰化类花色苷,主要花色苷为Petunidin-3-O-rutinoside(cis-p-coumaroyl)-5-O-glucoside。在所有供试黑果枸杞不同产地的材料中共检测到12种共有酚类化合物,其中Petunidin-3-O-rutinoside(cis-p-coumaroyl)-5-O-glucoside在所有供试黑果枸杞中的含量均最高,是黑果枸杞花色苷类多酚的主要成分。各产地样品HPLC特征图谱相似,有个别质量较差的情况存在;聚类参数不同,聚类结果略有差异。根据总酚含量、总黄酮含量、总花色苷含量聚类,聚为7类,甘肃民勤中渠、新疆喀什、宁夏贺兰12-1聚为第六类,新疆甘河子单独聚为第七类,该两类总酚、总黄酮及总花色苷含量均含量分别介于57.74—87.77 mg·g-1、41.52—55.46 mg·g-1和12.25—28.54 mg·g-1。总酚、总黄酮及总花色苷含量都很高,特别是新疆甘河子产地,可作为以多酚和花色苷含量为指标的优质良种材料。根据共有峰面积共聚为5类,其中宁夏贺兰12-1、青海格尔木乌图美仁、新疆甘河子为一类,该类峰面积很高,其中主成分峰面积介于2 350.84—3 092.94。新疆巴轮台镇单独一类,其主成分峰面积高达为3 579.96。结论 26个不同产地的黑果枸杞中多数富含多酚类物质,特别是酰化矮牵牛素花色苷含量丰富,是较好的开发富含花色苷类多酚的功能性食品资源。 | 闫亚美 戴国礼 冉林武 罗青 李晓莺 秦垦 巫鹏举 曾晓雄 曹有龙 | 2014 | 中国农业科学2014，47，22： | 68 |
| 7 | 熏煮香肠中脂肪、食盐、淀粉和水分含量对其质构的影响显示文摘质构是熏煮香肠的重要品质特征之一,脂肪、食盐、淀粉和水分含量是影响其质构的主要原辅料组成。文中应用质构剖面分析方法(textureprofileanalysis,TPA)对脂肪、食盐、淀粉和水分含量四因素的交互效应对熏煮香肠质构的影响进行了研究。结果显示,脂肪含量对熏煮香肠的硬度、粘聚性和咀嚼影响最大(调整R分别为129·34、0·051和33·14),而水分添加量对熏煮香肠的弹性影响最大(调整R为0·20)。通过感官评定与机械测定之间的相关分析,发现感官评定指标与机械测定的相应指标硬度、弹性、粘聚性和咀嚼性相关性较好(特别是硬度r=0·94和弹性r=0·89)。 | 董庆利 罗欣 屠康 | 2005 | 食品与发酵工业2005，31，5： | 51 |
| 8 | Cardiac autonomic neuropathy in patients with diabetes mellitus显示文摘Cardiac autonomic neuropathy(CAN)is an often overlooked and common complication of diabetes mellitus.CAN is associated with increased cardiovascular morbidity and mortality.The pathogenesis of CAN is complex and involves a cascade of pathways activated by hyperglycaemia resulting in neuronal ischaemia and cellular death.In addition,autoimmune and genetic factors are involved in the development of CAN.CAN might be subclinical for several years until the patient develops resting tachycardia,exercise intolerance,postural hypotension,cardiac dysfunction and diabetic cardiomyopathy.During its sub-clinical phase,heart rate variability that is influenced by the balance between parasympathetic and sympathetic tones can help in detecting CAN before the disease is symptomatic.Newer imaging techniques(such as scintigraphy)have allowed earlier detection of CAN in the pre-clinical phase and allowed better assessment of the sympathetic nervous system.One of the main difficulties in CAN research is the lack of a universally accepted definition of CAN;however,the Toronto Consensus Panel on Diabetic Neuropathy has recently issued guidance for the diagnosis and staging of CAN,and also proposed screening for CAN in patients with diabetes mellitus.A major challenge,however,is the lack of specific treatment to slow the progression or prevent the development of CAN.Lifestyle changes,improved metabolic control might prevent or slow the progression of CAN.Reversal will require combination of these treatments with new targeted therapeutic approaches.The aim of this article is to review the latest evidence regarding the epidemiology,pathogenesis,manifestations,diagnosis and treatment for CAN. | Gerasimos Dimitropoulos Abd A Tahrani Martin J Stevens | 2014 | World Journal of Diabetes2014，5，1： | 48 |
| 9 | A new approach to water resources system assessment——set pair analysis method显示文摘Most traditional assessment methods, which have complicated mathematic formulas, are difficult for calculation and application in water resources system assessment. A new approach to water resources system assessment, the set pair analysis method (SPAM), has been proposed based on the principle of set pair analysis (SPA). The basic ideals and steps of SPAM are discussed. The proposed method can take fuzzy property of threshold values for grade standards into full account and avoid determining the discrepancy uncertainty coefficient i or i1, i2, i3, … in SPA. The presented method is simple in concept, convenient to calculate and feasible for application. Two case studies of water resources assessment have been made. The results show that the proposed method is satisfactory. | WANG WenSheng JIN JuLiang DING Jing LI YueQing | 2009 | Science China(Technological Sciences)2009，52，10： | 43 |
| 10 | Dynamic changes of typeⅠ,Ⅲand N collagen synthesis and distribution of collagen-producing cells in carbon tetrachloride-induced rat liver fibrosis显示文摘AIM To find out the relationship between the gene transcription of different types ofprocollagen and the deposition of the relevant collagens in the liver tissue and to confirm the types of collagen producing cells in liverfibrogenesis.METHODS Dynamic changes of the expression of α1(Ⅰ ), α1 (Ⅲ ) and α1 (Ⅳ) procollagen mRNAand relevant collagens and the distribution ofcollagen producing cells during liver fibrogenesis of rat induced by CCl4 (20 weeks)were investigated with Northern blot analysis,in situ hybridization and immunohistochemicaltechniques.RESULTS The increased expression of α1 (Ⅲ)procollagen mRNA by Northern blot analysis was the most predominant one among the threemRNAs during fibrogenesis. However, theenhanced expression of al (Ⅳ) procollagenmRNA occurred very early while the expressionof α1 (Ⅰ) mRNA was not enhanced much until themiddle stage of the exPeriment. D6smin (Dm)positive hepatic stellate cells (HSCs) and fewmyofibroblasts (MFs) in and around the necrotic areas expressed α1 (Ⅰ), α1 (Ⅲ) and α1 (Ⅳ)procollagen mRNA signals detected by in situhybridization at the early stage of theexperiment. All the three procollagen mRNAsignals thereafter mainly localized in fibroblasts (Fbs) and MFs in fibrotic septa during the middle and late stages of fibrosis, which distributedparallel to the corresponding collagens detected by immunohistochemical study. ln addition, the endothelial cells of sinusoids and the small blood vessels within the septa also showed α1(Ⅳ) procollagen mRNA and type Ⅳ collagen expressionCONCLUSION It is considered that 'HSC-MF-Fb' effect cell system is the major cellularsource of collagen production in liver fibrosis, in which HSCs are collagen producing precursor cells in the early liver fibrogenesis, thereafter the synthesis of type Ⅰ, Ⅲ and Ⅳ collagens (Col Ⅰ, Col Ⅲ and Col Ⅳ) mainly derives fromMFs and Fbs, which play a very important role in the progress of liver fibrosis. The endothelialcells along sinusoids, as another source of Col Ⅳ production, might participate in the capillization of liver sinusoids. | DU Wei-Dong ZHANG Yue-E ZHAl Wei-Rong and ZHOU Xiao-Mei(Department of Pathology, Shanghai Medical University, Shanghai200032, China)(National Laboratory for Oncogenes and Related Genes, ShanghaiCencer Institute)See invited commentary on page 388 | 1999 | World Journal of Gastroenterology1999，5，5： | 47 |
| 11 | 数字实验室——LIMS未来发展趋势显示文摘LIMS出现于 80年代 ,90年代在西方迅速普及。目前国内也掀起了LIMS的热潮。LIMS从最初仅仅完成数据存储、有限的网络功能 ,发展到现在可以处理海量数据 ,具备完善的管理职能 ,并且能够运行于Internet之上 ,极大地提高了实验室的运行效率 ,大幅度节约了实验室的运营成本。今后 ,LIMS将朝着专家系统、集成最新、最先进的专用分析方法的方向发展 ,结合GIS技术、宽带网络技术以及电子商务技术 ,成为一个功能极其完备的复杂系统。在文章最后 ,针对LIMS用户提出了一些有意义的建议。 | 冯金辉 | 2002 | 现代科学仪器2002，19，2： | 49 |
| 12 | Prognostic factors of refractory NSCLC patients receiving anlotinib hydrochloride as the third-or further-line treatment显示文摘Objective:Anlotinib hydrochloride is a multitarget tyrosine kinase inhibitor that targets vascular endothelial growth factor receptor,fibroblast growth factor receptor,platelet-derived growth factor receptor,c-Kit,and c-MET;therefore,it exhibits both antitumor and anti-angiogenetic activities.A phase III trial has shown that anlotinib improved progression-free survival(PFS)and overall survival(OS)in patients with advanced non-small cell lung cancer(NSCLC),who presented with progressive disease or intolerance after standard chemotherapy.This study aimed to analyze the characteristics of patients receiving anlotinib treatment to determine the dominant populations who are fit for the treatment.Methods:Data were collected from March 2015 to January 2017 from a randomized,double-blind,placebo-controlled,multicenter,phase III trial of anlotinib(ALTER0303).A total of 437 patients were enrolled and randomly allocated(2:1)to the anlotinib and placebo groups.Kaplan–Meier analysis and log-rank test were performed to compare PFS and OS.Cox proportional hazards model was adopted for multivariate prognostic analysis.Results:Multivariate analysis indicated that high post-therapeutic peripheral blood granulocyte/lymphocyte ratio and elevated alkaline phosphatase levels were independent risk factors for PFS.Meanwhile,elevated thyroid-stimulating hormone,blood glucose,and triglyceride levels;hypertension;and hand–foot syndrome were independent protective factors of PFS.High posttherapeutic peripheral blood granulocyte/lymphocyte ratio,an Eastern Cooperative Oncology Group(ECOG)score≥2,and the sum of the maximal target lesion length at baseline were independent risk factors of OS,and hypertriglyceridemia was an independent protective factor of OS.Conclusions:This study preliminarily explored the possible factors that affected PFS and OS after anlotinib treatment in patients with advanced refractory NSCLC,and the baseline characteristics of the therapeutically dominant populations were then identified. | Jing Wang Yizhuo Zhao Qiming Wang Li Zhang Jianhua Shi Zhehai Wang Ying Cheng Jianxing He Yuankai Shi Hao Yu Yang Zhao Weiqiang Chen Yi Luo Xiuwen Wang Kejun Nan Faguang Jin Jian Dong Baolan Li Zhujun Liu Baohui Han Kai Li | 2018 | Cancer Biology & Medicine2018，15，4： | 46 |
| 13 | High-efficiency design of a mixed-flow pump显示文摘High-efficiency design of a mixed-flow pump has been carried out based on numerical analysis of a three-dimensional viscous flow.For analysis,the Reynolds-averaged Navier-Stokes equations with a shear stress transport turbulence model were discretized by finite-volume approximations.Structured grid system was constructed in the computational domain,which has O-type grids near the blade surfaces and H/J-type grids in other regions.The numerical results were validated with experimental data for the heads and hydraulic efficiencies at different flow coefficients.The hydraulic efficiency at the design flow coefficient was evaluated with variation of the geometric variables,i.e.,the area of the discharge and length of the vane in the diffuser.The result has shown that the hydraulic efficiency of a mixed-flow pump at the design condition is improved by the modification of the geometry. | KIM Jin-Hyuk AHN Hyung-Jin KIM Kwang-Yong | 2010 | Science China(Technological Sciences)2010，53，1： | 39 |
| 14 | Optimization of the head shape of the CRH3 high speed train显示文摘Aiming at optimizing the head shape of the CRH3 high speed train, an efficient optimization approach is proposed. The CFD analysis by solving Navier-Stokes equations is coupled with optimization calculation based on the multi-objective genetic algorithm, meanwhile the arbitrary shape deformation technique (ASD) is also introduced into the design flow, which greatly shortens the time consumption for geometry regeneration and flow field remeshing. As a result, the efficiency of the optimization calculation is highly improved. Statistical analysis is done to the designs in the design space, and the correlation between the design variables and the objective is studied to find out the key variables that most affect the objective. Response surface analysis is also performed to get the nonlinear relationship between the key design variables and the objective with the Kriging algorithm. Finally, after the optimization, an aerodynamic performance comparison between the optimal shape and the original shape reveals that the original shape of CRH3 high speed train owns a very stable aerodynamic performance and can be trustingly used in industry. | SUN ZhenXu1, SONG JingJing1 & AN YiRan1 College of Engineering, State Key Laboratory for Turbulence and Complex Systems, Peking University, Beijing 100871, China | 2010 | Science China(Technological Sciences)2010，53，12： | 35 |
| 15 | A Large-Scale Palaeozoic Dextral Ductile Strike-Slip Zone:the Aqqikkudug-Weiya Zone along the Northern Margin of the Central Tianshan Belt,Xinjiang,NW China显示文摘The nearly E-W-trending Aqqikkudug-Weiya zone, more than 1000 km long and about 30 km wide, is an important segment in the Central Asian tectonic framework. It is distributed along the northern margin of the Central Tianshan belt in Xinjiang, NW China and is composed of mylonitized Early Palaeozoic greywacke, volcanic rocks, ophiolitic blocks as a melange complex, HP/LT-type bleuschist blocks and mylonitized Neoproterozoic schist, gneiss and orthogneiss. Nearly vertical mylonitic foliation and sub-horizontal stretching lineation define its strike-slip feature; various kinematic indicators, such as asymmetric folds, non-coaxial asymmetric macro- to micro-structures and C-axis fabrics of quartz grains of mylonites, suggest that it is a dextral strike-slip ductile shear zone oriented in a nearly E-W direction characterized by 'flower' strusture with thrusting or extruding across the zone toward the two sides and upright folds with gently plunging hinges. The Aqqikkudug-Weiya zone experienced at least two | CHARVET Jacques | 1999 | Acta Geologica Sinica(English Edition)1999，73，2： | 36 |
| 16 | 中医通法浅析显示文摘'通'为常,'不通'则病。通法有广义和狭义之分。狭义通法是指宣通郁滞、通利二便。广义通法是指疏通脏腑经络气机,消除体内壅滞,畅行气血津液。 | 汤尔峰 姜惟 | 2012 | 中国中医急症2012，21，3： | 38 |
| 17 | Quantitative and HPLC fingerprint analysis combined with chemometrics for quality evaluation of Codonopsis Radix processed with different methods显示文摘Objective: To observe the effect of different processing methods on the quality of Codonopsis Radix and provide data reference for the reasonable processing in the producing areas.Methods: An analytical strategy that combined quantitative and HPLC fingerprint analysis with chemometrics was developed for the quality evaluation of Codonopsis Radix. Lobetyolin, polysaccharide, sucrose,glucose, and fructose were detected simultaneously in Codonopsis Radix samples treated with different processing methods including dryer-drying, sun-drying, shade-drying, sulfur fumigation, and kneading,etc.Results: The results showed that different processing methods had an obvious impact on the quality of Codonopsis Radix. Sun-drying or shade-drying was recommended, and sulfur fumigation should be avoided. And dryer-drying at 40 °C-50 °C was suggested to meet the requirement for large scale processing. In addition, based on the content of polysaccharide, glucose, and fructose, it was advised to knead for three times. As for lobetyolin and sucrose, kneading should be avoided.Conclusions: Our findings demonstrated that processing method had a big impact on the quality of Codonopsis Radix. Based on the results, the appropriate processing methods directed at different requirements were recommended. The study could lay a foundation for the reasonable processing of Codonopsis Radix in producing areas. | Shi-man Gao Jiu-shi Liu Min Wang Ting-ting Cao Yao-dong Qi Ben-gang Zhang Hai-tao Liu Xiao-bo Sun Pei-gen Xiao | 2019 | Chinese Herbal Medicines2019，11，2： | 39 |
| 18 | Effect of cholecystokinin on cytokines during endotoxic shock in rats显示文摘AIM To study the effect of cholecystokinin-octapeptide (CCK-8) on systemic hypotension and cytokine production in lipopolysaccharide (LPS)-induced endotoxic shock (ES) rats.``METHODS The changes of blood pressure were observed using physiological record instrument in four groups of rats: LPS (8 mg. kg-1, iv) induced ES; CCK-8 (40 μg.kg- 1 iv) pretreatment 10 min before LPS (8 mg. kg- 1);CCK-8 (40 μg.kg-1, iv) or normal saline (control) groups.Differences in tissue and circulating specificity of the proinflammatory cytokines (TNF-a, IL-l3 and IL-6) were assayed with ELISA kits.``RESULTS CCK-8 reversed LPS-induced decrease of mean artery blood pressure (MABP) in rats. Compared with control, LPS elevated the serum level of IL-6 significantly (3567_-687 ng.L-1 vs 128_+22 ng.L-1, P<0.01), while contents of TNF-a and IL- lβ elevated significantly (277 _± 86ng.L-1 vs not detectable and 43 ± 9 ng.L-1 vs notdetectable, P<0.01) but less extent than IL-6, CCK-8significantly inhibited the LPS-induced increase in serum TNF-a, IL-lβ and IL-6. LPS elevated spleen and lung content of IL-Iβ significantly (5184 ± 85 ng.L-1 vs 1047 ±21 ng.L-1 and 4050 ± 614 ng.L-1 vs not detectable,P<0,01). while levels of TNF-a and IL-6 also rosesignificantly but in less extent than IL-lβ. CCK-8 inhibited the LPS-induced increase of the cytokines in spleen and lung. in the heart, CCK-8 significantly inhibited LPS.induced increase of TNF-a (864 ± 123 ng. L-1 in CCK-8 +LPS group vs 1599_-227 ng-L-1 in LPS group, P<0.01),and IL-lβ (282 ± 93 ng-L-1 in CCK-8 + LPS group vs 621 ±145 ng.L-1 in LPS group, P<0.01).``CONCLUSION CCK-8 reverses ES, which may be relatedto its inhibitory effect on the overproduction of cytokines. | Yi-Ling Ling~1 Ai-Hong Meng~1 Xiao-Yun Zhao~1 Bao-En Shan~2 Jun-Lan Zhang~1 Xiao-Peng Zhang~3 1 Department of Pathophysiology,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China2 Research Center of Fourth Hospital,Hebei Medical University,Shijiazhuang 050000,Hebei Province,China3 Department of Chest Surgery of Hebei Provincial People’s Hospital,Shijiazhuang 050000,Hebei Province,China | 2001 | World Journal of Gastroenterology2001，7，5： | 31 |
| 19 | Starch grain analysis reveals function of grinding stone tools at Shangzhai site, Beijing显示文摘Prehistoric groundstone tools including slabs (metate, mo-pan) and mullers (mano, mo-bang) are often considered to be tools that were used to process cereals in ancient China. Most archaeologists believe that groundstone tools are indicators of an agrarian economy. This study employed starch grain analysis to examine a slab and a muller excavated from the Shangzhai Site (7500-7000 cal a BP), Beijing. Identifiable starch grains recovered from the stone tools were classified into 9 genera and 12 species, most of which were from acorns and foxtail millets. Remains from the oak species Q. mongolicus, Q. acuttssima, and Q. dentate were identified as well as a few starch grains from broomcorn (Panicum miliaseum) and bean (Vigna sp.). Other starch grains were from unidentifiable roots and grasses. A variety of starch grains indicates that the grinding stone tools were used to process not only cereals, but also other seeds or roots that had to be ground or husked, thus bringing into question the aforementioned cereal hypothesis. Because the numbers of starch grains from nuts and millets were so large, both gathering and cultivation might have been important economic patterns before 7000 years ago in Beijing area. Moreover, the starch remains are derived from plants that indicate that the paleoclimate of Beijing was wetter and warmer than today’s. | YANG XiaoYan YU JinCheng Lü HouYuan CUI TianXing GUO JingNing GE QuanSheng | 2009 | Science China Earth Sciences2009，52，8： | 32 |
| 20 | Analysis of long non-coding RNA expression profiles in gastric cancer显示文摘AIM: To investigate the expression patterns of long non-coding RNAs (lncRNAs) in gastric cancer. METHODS: Two publicly available human exon arrays for gastric cancer and data for the corresponding normal tissue were downloaded from the Gene Expression Omnibus (GEO). We re-annotated the probes of the human exon arrays and retained the probes uniquely mapping to lncRNAs at the gene level. LncRNA expression profiles were generated by using robust multi-array average method in affymetrix power tools. The normalized data were then analyzed with a Bioconductor package linear models for microarray data and genes with adjusted P -values below 0.01 were considered differentially expressed. An independent data set was used to validate the results. RESULTS: With the computational pipeline established to re-annotate over 6.5 million probes of the Affymetrix Human Exon 1.0 ST array, we identified 136053 probes uniquely mapping to lncRNAs at the gene level. These probes correspond to 9294 lncRNAs, covering nearly 76% of the GENCODE lncRNA data set. By analyzing GSE27342 consisting of 80 paired gastric cancer and normal adjacent tissue samples, we identified 88 lncRNAs that were differentially expressed in gastric cancer, some of which have been reported to play a role in cancer, such as LINC00152, taurine upregulated 1, urothelial cancer associated 1, Pvt1 oncogene, small nucleolar RNA host gene 1 and LINC00261. In the validation data set GSE33335, 59% of these differentially expressed lncRNAs showed significant expression changes (adjusted P -value < 0.01) with the same direction. CONCLUSION: We identified a set of lncRNAs differentially expressed in gastric cancer, providing useful information for discovery of new biomarkers and therapeutic targets in gastric cancer. | Wei-Jun Cao Hai-Lu Wu Bang-Shun He Yu-Shu Zhang Zhen-Yu Zhang | 2013 | World Journal of Gastroenterology2013，19，23： | 33 |
您所在的机构暂未开通此部分的功能，请联系管理员