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Functional genomics of maize submergence tolerance and cloning of the related gene Sicyp51

查看全文 作　　者：TANG Wanhu1, ZHANG Zuxin1,2, ZOU Xiling1 & ZHENG Yonglian1 1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;2. College of Life Science, Yangtze University, Jingzhou 434025, China 高影响力作者 出　　处：《Science China(Life Sciences)》索引2005年第48卷第4期,共9页高影响力期刊 基　　金：This work was supported by the National Natu-ral Science Foundation of China(Grant No.30370886),Hubei Natural Science Fund(Grant No.2003ABA094);the Doctoral Progr am F oundation of the Ministry of Education(Grant No.20030504021). 摘　　要：In this study, SSH (Suppression Subtractive Hybridization) and cDNA microarray were used to identify genes associated with waterlogging response of maize roots. Mo17 and Hz32 are two maize inbred lines with differential tolerance to hypoxia. Seedlings of the inbred lines with two leaves were submerged in hypoxia buffer. SSH libraries were constructed with cDNA samples from roots. Both forward and reverse subtractions were performed for each inbred line, and 105 positive clones induced by hypoxia were selected by differential screening. The treated and control message RNA were hybridized with the cDNA microarray of Mo17, sequen-tially, 57 of 3-fold differentially expressed clones were obtained. A total of 162 positive clones were all sequenced. Bioinformatics analysis showed these positive clones represent 85 TUGs, including genes involved in several biochemistry pathways, such as glycolysis, protection, signal transduction, cell construction and energy metabolism and 41 EST with unknown function. Comparison between Mo17 and Hz32 indicates that genes related to hypoxia tolerance have different expression patterns in submerged roots. Several positive clones’ expression patterns were revealed by Northern or RT-PCR, and a new gene (Sicyp51), which may contribute to hy-poxia tolerance, was identified. 关 键 词：maize, WATERLOGGING tolerance, functional genomics, Submergence-inducible CYP51.
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