Acquirement and characterization of a carotenoid mutant (GM309) of Rhodobacter sphaeroides 601

查看全文 作　　者：LIU Yuan ZHANG Wei WU Yongqiang XU [1]Chunhe

机构地区：[1]Shanghai Institute of Plant Physiology & Ecology, Shanghai institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China Shanghai Institute of Plant Physiology & Ecology, Shanghai institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China Shanghai Institute of Plant Physiology & Ecology, Shanghai institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China Shanghai Institute of Plant Physiology & Ecology, Shanghai institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

出　　处：《Science China(Life Sciences)》索引2004年第47卷第1期,共7页

摘　　要：A green mutant was obtained among the chemically induced mutants of Rhodobacter sphaeroides 601 (RS601) and named GM309. A blue shift of 20 nm of the carotenoid absorption spectrum was found in the light-harvesting complex II (LH2) of GM309. Different from LH2 of RS601, it was found that the carotenoids in GM309-LH2 changed to be neurosporene by mutation. Neurosporene lacks a conjugate double bond, compared with the spheroidene in RS601-LH2 which has ten conjugate double bonds. As shown by absorption and circular dichroism spectroscopy, the overall structure of GM309-LH2 is little affected by this change. From fluorescence emission spectra, it is found that GM309-LH2 can transfer energy from carotenoids to Bchl-B850 without any change in efficiency. But the efficiency of energy transfer from B800 to B850 in GM309-LH2 is decreased to be 42% of that of the native. This work would provide a novel method to investigate the mechanism of excitation energy transfer in LH2. 关键词：LIGHT-HARVESTING complex, carotenoid, EXCITATION energy transfer, fluorescence.

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Acquirement and characterization of a carotenoid mutant (GM309) of Rhodobacter sphaeroides 601

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