Increased expression of osteoprotegerin in vascular smooth muscle cells from spontaneously hypertensive rats

查看全文 作　　者：Yongshan MOU Tianhua LEI Luning ZHAO Xiaojun ZHU Mingui FU Yuqing E [1,2]CHEN 机构地区：[1]Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive SW, Atlanta, GA 30310, USA Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive SW, Atlanta, GA 30310, USA Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive SW, Atlanta, GA 30310, USA Cardiovascular Research Institute, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100083, China Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive SW, Atlanta, GA 30310, USA Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive SW, Atlanta, GA 30310, USA;[2]Cardiovascular Research Institute, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100083, China; 出　　处：《Journal of Geriatric Cardiology》索引2004年第1卷第1期,共6页 摘　　要：Background Osteoprotegerin (OPG) is a secreted protein of the tumor necrosis factor receptor family, which regulates bone mass by inhibiting osteoclast differentiation and activation. Although OPG is expressed ubiquitously and abundantly in many tissues and cell types including vascular cells, the role of OPG in other tissues is unknown.Our previous studies demonstrated that OPG was highly expressed in vascular smooth muscle cells (VSMC) and upregulated during vascular lesion formation. Methods and Results We documented, by Northern blot analysis,that the expression of OPG was more prevalent in the aorta and cultured VSMC from spontaneously hypertensive rats (SI-IR) compared to Wistar-Kyoto rats (WKY). In addition, we found that the expression of Angiotensin II (Ang II)type I receptor (AT1R) in SHR VSMC was at significantly increased levels than in WKY VSMC. Furthermore, Ang II potently induced the expression of OPG in VSMC in a time- and dose-dependent manner through the AT1R signaling pathway. Conclusions OPG expression was substantially greater in SHR VSMC, suggesting that OPG may be an important determinant of vascular remodeling in SHR. 关 键 词：OSTEOPROTEGERIN HYPERTENSION VASCULAR SMOOTH MUSCLE cells gene expression