Separation and Purification of Thrombin-like Enzymes by Affinity Adsorbents

查看全文 作　　者：[1]杨青;Xu [1]Xiaoming;Hu [1]Xuejun;Gao [1]Xiaorong;Dong [2]Xinyan;Su [3]Zhiguo;An [1]Lijia

机构地区：[1]Bioengineering Institute, Dalian University of Technology,;[2]Takara Biotech Dalian Co. Ltd., Dalian Development Zone,;[3]State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, CAS,;

出　　处：《High Technology Letters》索引2001年第7卷第4期,共3页

摘　　要：An affinity adsorbent, benzamidine Sepharose 4B, was used to separate and purify thrombin like enzymes. The p aminobenzamidine as a specific ligand was coupled to the matrix-Sepharose 4B. The recombinant thrombin like enzyme-defibrase was used as a model in order to evaluate the efficiency of this biospecific affinity adsorbent. The homogeneity of the enzyme preparation was comfirmed as one band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. 关键词：Affinity chromatography LIGAND Benzamidine PURIFICATION Thrombin like enzyme

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Separation and Purification of Thrombin-like Enzymes by Affinity Adsorbents

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