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Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cells

查看全文 作　　者：Hui Qiang1,2,Guang-Sheng Wang3,Chen Zhang1,Zhi-Bin Shi1,Li-Hong Fan1,Kun-Zheng Wang1 1.Department of Orthopedics,the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710004;2.Department of Orthopedics,Shaanxi Province People’s Hospital,Xi’an 710068;3.Department of Orthopedics,Huashan Hospital,Baoji 721000,China. 高影响力作者 出　　处：《Journal of Pharmaceutical Analysis》索引2010年第22卷第1期,共5页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China(No.30600624) 摘　　要：Objective To investigate the effects of Panax notoginseng saponins(PNS)on hydrogen peroxide(H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells(BMSCs).Methods BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent method.The cultured BMSCs were divided into three groups:normal control,H2O2 treatment(100μmol/L),and PNS pretreatment(0.1g/L).Intracellular reactive oxygen species(ROS)levels as the index of oxidative stress were measured by using 2'7'-dichlorodihydrofluorescein diacetate.Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/PI.The protein expression of Bax in BMSCs was analyzed by Western blotting.Activity of caspase-3 enzyme was measured by spectrofluorometry.Results Pretreatment with PNS significantly decreased intracellular ROS level induced by H2O2(P<0.01).PNS markedly attenuated H2O2-induced apoptosis rate from 38.68% to 19.24%(P<0.01).PNS reversed H2O2-induced augmentation of Bax expression.Furthermore,PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2(P<0.01).Conclusion PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity. 关 键 词：Panax notoginseng saponins reactive oxygen species bone marrow stromal cell APOPTOSIS BAX
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