Triptolide prolonged allogeneic islet graft survival in chemically induced and spontaneously diabetic mice without impairment of islet function

查看全文 作　　者：Xin, Ming-[1,2]Jun;Cui, Shi-[1,3]Hua;Liu, [1]Shuang;Sun, Hai-[1]Chen;Li, [1]Fei;Sun, Jia-[1]Bang;Luo, [1]Bin

机构地区：[1]Capital Med Univ, Dept Gen Surg, Xuanwu Hosp, Beijing 100053, Peoples R China;[2]Qingdao Municiple Hosp, Dept Hepatobiliary Surg, Qingdao 266011, Peoples R China;[3]Peking Univ, Sch Clin, Shougang Hosp, Dept Surg, Beijing 100144, Peoples R China;

出　　处：《Hepatobiliary & Pancreatic Diseases International》索引2010年第9卷第3期,共7页

摘　　要：BACKGROUND:Triptolide(TPT)is a diterpenoid triepoxide extracted from the Chinese herb Tripterygium wilfordii Hook.F.It exhibits potent immunosuppressive and anti-inflammatory properties.This study was undertaken to investigate its effects on prolongation of islet allograft survival in rodents.Additionally,we investigated whether TPT would be toxic to islet function in vivo.METHODS:We transplanted BALB/c islets to either chemically induced diabetic C57BL/6 mice or spontaneously diabetic nonobese diabetic(NOD)mice.TPT was injected within 2 weeks or continuously,until rejection,in the two combinations.Then, we evaluated the toxicity of TPT on islet function by daily injection to naive BALB/c or diabetic BALB/c that was cured by syngeneic islet transplantation under the kidney capsule.Mice injected with cyclosporine A(CsA)or vehicle served as controls.Intraperitoneal glucose tolerance tests(IPGTTs)performed at 4 and 8 weeks in the na?ve BALB/c group,and at 2,4,6,and 8 weeks in the syngeneic transplanted group.RESULTS:The medium survival time of islets allograft from TPT treated C57BL/6 and NOD recipients were 28.5 days(range 24-30 days,n=10)and 33.0 days(range 15-47 days,n=6), respectively,and they were significantly different from those of the vehicle treated controls,which were 14.0 days(range 13-16 days,n=6)and 5.0 days(range 4-10 days,n=6),respectively(all P<0.0001).The IPGTT demonstrated that there was no difference between the TPT treated and vehicle treated groups, either in the normal or syngeneic transplanted islet BALB/c mice.However,CsA injection impaired islet function in both normal and syngeneic transplanted mice as early as 4 weeks.CONCLUSION:TPT prolonged islets allograft survival in a chemically induced diabetic or an autoimmune diabetic murine model without impairment of islet function. 关键词：glucose tolerance test IMMUNOSUPPRESSION ISLET transplantation NON-OBESE diabetic mice TRIPTOLIDE

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Triptolide prolonged allogeneic islet graft survival in chemically induced and spontaneously diabetic mice without impairment of islet function

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