Analysis of gene expression profiles in pancreatic carcinoma by using cDNA microarray

查看全文 作　　者：Xian-Jun Yu;Jiang Long;De-Liang Fu;Qun-Hua Zhang;Quan-Xin Ni the Center for Pancreatic Cancer, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China

出　　处：《Hepatobiliary & Pancreatic Diseases International》索引2003年第2卷第3期,共4页

摘　　要：OBJECTIVES: To survey the gene expression profiles in pancreatic carcinoma by using cDNA microarrayand detect target genes for further study.METHODS: Three mixed samples from 2 cases of normal pancreatic tissue and 4 cases ofmoderate-differentiated pancreatic carcinoma were studied by means of cDNA microarray consisting of18 000 genes.RESULTS: 1484 and 1353 different expressed genes were observed in two cancer samples respectively.We identified 455 genes altered with the same tendency in both samples, including 102 up-regulated and353 down-regulated genes. There were 274 known genes and 181 unknown genes; 27.8% and 52.0%genes respectively had an expression level in cancer that was 2-fold higher or lower than that in normalsamples. Tumor suppressor genes, growth factors and receptor genes, signal conduction genes,transcription factor genes were identified.CONCLUSIONS: cDNA microarray is an efficient and high-throughout method to investigate geneexpression profiles in pancreatic carcinoma. MBD1, EDG1 and gene hypermethylation mechanism wouldplay an important role in the pathogenesis of pancreatic carcinoma. 关键词：PANCREATIC CARCINOMA CDNA MICROARRAY gene expression profiles

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Analysis of gene expression profiles in pancreatic carcinoma by using cDNA microarray

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