Anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica

查看全文 作　　者：Mohan [1]CG;Deepak [2]M;Viswanatha [3]GL;Savinay [4]G;Hanumantharaju [1]V;Rajendra [5]CE;Praveen D [6]Halemani 机构地区：[1]Department of Pharmacognosy,Government College of Pharmacy,Bangalore-560027;[2]Department of Phytochemistry,Natural Remedies Pvt,Ltd.Bangalore-560 100;[3]Department of Pharmacology,PES College of Pharmacy,Bangalore-560050;[4]Deparment of Pharmaceutics,Government College of Pharmacy,Bangalore-560027;[5]Drug Testing Laboratory,Drugs Control Department,Palace road,Bangalore-560001;[6]Department of Pharmaceutics,Government College of Pharmacy,Bangalore-560 027 出　　处：《Asian Pacific Journal of Tropical Medicine》索引2013年第6卷第4期,共4页 摘　　要：Objective:To evaluate the anti-oxidani and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.Methods:In vitro DPPH radical scavenging activity and lipoxygenase(LOX) inhibition assays were used to evaluate the anti-oxidant and anti-inflammatory activities respectively.Methanolic extract(MEMI),successive water extract (SWMI) and ethyl acetate fraction(EMEMI),n-butanol fraction(BMEMI) and water soluble fraction (WMEMI) of methanolic extract were evaluated along with respective reference standards. Results:In in ritro DPPH radical scavenging activity,the MEMI,EMEMI and BMEMI have offered significant antioxidant activity with IC₅₀ values of 13.37.3.55 and 14.19μig/mL respectively.Gallic acid,a reference standard showed significant antioxidant activity with IC? value of 1.88 and found to be more potent compared to all the extracts and fractions.In m vitro LOX inhibition assay,the MEMI,EMEMI and BMEMI have showed significant inhibition of LOX enzyme activity with IC₅₀ values of 96.71.63.21 and 107.44μg/mL respectively.While,reference drug Indometlhacin also offered significant inhibtion against LOX enzyme activity with IC₅₀ of 57.75.Furthermore,MEMI was found to more potent than SWMI and among the fractions EMEMI was found to possess more potent antioxidant and anti-inflammatory activity.Conclusions:These findings suggest that the MEMI and F.MEMT possess potent anti-oxidani and anti-inflammatory activities in in vitro conditions. 关 键 词：MANGIFERA INDICA LIPOXYGENASE DPPH assay ANTIOXIDANTS Anti-inflammatory