维普中文期刊产品整合服务

Simultaneous detection and differentiates of Brucella abortus and Brucella melitensis by combinatorial PCR

查看全文 作　　者：Reza [1]Mirnejad;Reza Hosseini [1]Doust;Reza [1]Kachuei;Seied Mojtaba [2]Mortazavi;Mehdi [3]Khoobdel;Ali [1]Ahamadi 高影响力作者 机构地区：[1]Molecular Biology Research Center,Baqiyatallah University of Medical Sciences,Tehran,Iran;[2]Health Station,Kerman University of Medical Sciences,Kerman,Iran;[3]Health Research Center,Baqiyatallah University of Medical Sciences,Tehran,Iran高影响力机构 出　　处：《Asian Pacific Journal of Tropical Medicine》索引2012年第5卷第1期,共5页高影响力期刊 基　　金：supported by Molecular Biology Research Center,Baqiyatallah University of Medical Sciences,with grant number BMSU/MBRC- 89-009 摘　　要：Objective:To evaluate simultaneous detection and differentiates of Brucella abortus(B.abortus) and Brucella melitensis(B.melitensis) through the combinatorial POR method.Methods:This study was designed using three primers that could simultaneously identify and differentiate two major species of pathogenic Brucella in humans and animals.Identification and differentiation of each species using the size of the PCR product were determined.To determine the specifieity of the method,bacteria close to the genus Brucella were used.Finally,to confirm PCR products.In addition to the products sequence,RFLP was performed on PCR products using restriction enzymes.Results:The method of optimized combinatorial PCR in this study could simultaneously detect and differentiate B.abortus and B.melitensis with high specificity and sensitivity in clinical samples.Differentiation of species is based on the resulting bands: therefore,the band 494 bp for B.abortus and 733 bp for B.melitensis were obtained.RFLP and sequencing results confirmed PCR results.Conclusions:The results of this study shows that without routine diagnostic methods such as culture and serology tests,using the molecular method of combinatorial PCR,important species of Brucella can be simultaneously identified and differentiated in clinical samples. 关 键 词：BRUCELLA ABORTUS BRUCELLA MELITENSIS Combinatorial PCR
相关文献

参考文献(23)

引证文献(5)

耦合文献(3)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费