Higher production of tumor necrosis factor alpha in hemozoin-fed human adherent monocytes is dependent on lipidic component of malarial pigment:new evidences on cytokine regulation in Plasmodium falciparum malaria

查看全文 作　　者：Prato [1]Mauro;Gallo [1]Valentina;Arese [1]Paolo

机构地区：[1]Department of Genetics,Biology and Biochemistry,University of Torino Medical School

出　　处：《Asian Pacific Journal of Tropical Medicine》索引2010年第3卷第2期,共5页

基　　金：supported in the context of the Italian Malaria Network by grants from Compagnia di San Paolo-IMI and from Regione Piemonte,Ricerca Sanitaria Finalizzata 2008bis to PA 摘　　要：Objective:To investigate whether the increase of tumor necrosis factor alpha is dependent on lipidic component of malarial pigment.Methods:Adherent human monocytes were fed for 3 hours with different meals(native hemozoin;lipid free hemozoin;and control latex particles),then tumor necrosis factor alpha was monitored in cell supernatants up to 48 hours through western blotting or specific enzyme-linked immunoadsorbent assay.In selected experiments,unfed monocytes were treated with different doses of 15(S,R)-hydroxy-6,8,11,13-eicosatetraenoic acid or 4-hydroxynonenal instead of phagocytosis.Results:Hemozoin-fed monocytes produced higher levels of tumor necrosis factor alpha than unstimulated and latex-fed cells, while lipid-free hemozoin did not reproduce these results.Additionally,hemozoin effects were mimicked dose-dependently by 15(S,R)-hydroxy-6,8,11,13-eicosatetraenoic acid,but not by 4-hydroxynonenal.Conclusions:Present data suggest an essential role for lipids in hemozoindependent enhanced release of tumor necrosis factor alpha from monocytes,and 15(S,R)hydroxy -6,8,11,13-eicosatetraenoic acid could be one possible specific mediator. 关键词：HEMOZOIN PLASMODIUM FALCIPARUM Malaria Monocyte PHAGOCYTOSIS Tumor necrosis factor alpha(TNFalpha) 15(S,R)-hydroxy-6,8,11,13-eicosatetraenoic acid(15-HETE) Matrix metalloproteinase-9(MMP-9)

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Higher production of tumor necrosis factor alpha in hemozoin-fed human adherent monocytes is dependent on lipidic component of malarial pigment:new evidences on cytokine regulation in Plasmodium falciparum malaria

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