Evaluation of berries of Phytolacca dodecandra for growth inhibition of Histoplasma capsulatum var.farciminosum and treatment of cases of epizootic lymphangitis in Ethiopia

查看全文 作　　者：Negesse [1,2]Mekonnen;Eyasu [1]Makonnen;Nigatu [3]Aklilu;Gobena [2]Ameni

机构地区：[1]Department of Pharmacology,School of Medicine,Addis Ababa University;[2]Aklilu Lemma Institute of Pathobiology,Addis Ababa University;[3]Society for the Protection of Animals Abroad(SPANA),School of Veterinary Medicine,Addis Ababa University

出　　处：《Asian Pacific Journal of Tropical Biomedicine》索引2012年第2卷第7期,共6页

基　　金：supported by the Graduate Program the Addis Ababa University and Aklilu Lemma Institute of Pathobiology Addis Ababa University(grant No.CAOR/PY-059/2011) 摘　　要：Objective:To evaluate the berries of Phytolacca dodecundra(P.dodecandra) for its effect on Histoplasma cupsulatum var.farciminosum(HCF) and for the treatment of cases of epizootic lymphangitis(ELi.Methods:Samples were collected from un-ruptured nodules of cases of EL at Debre Zeit and Akaki(central Ethiopia).Mycologieal culture and isolation of HCF were performed at the Akliln Lemma Institute of Pathobiology.Phytochemical screening was done for n-butanol extract of P.dodecandra to delect alkaloids,saponins,phenolic compounds and flavonoids.The minimum inhibitory concentrations(MICs) and minimum fungicidal concentrations(MFCs) ol aqueous and n-butanol extracts of P.dodecandra against FICF were determined by agar dilution assay.For the in vivo trial.5%simple ointment was prepared from n-butanol extract and applied topically to 24(twelve early and twelve moderate) cases of F.L.Results:Phytochemical screening showed that n-butanol extract ol P.dodecandra was positive lor alkaloids.saponins and phenolic compounds but negative for flavonoids.The MFCs of n-butanol and aqueous extracts of P.dodecandra were(0.039%-0.078%) and(0.625%-1.250%),respectively.The MFCs of n-butanol and aqueous extracts of P.dodecandra were(0.078%t-0.156%)and(1.250%-2.500%),respectively.The MIC and MFC of ketoconazole(positive control) was(1.200×10^(-5)%-2.500×10^(-5)%) and(5.000× 10^(-5)%-1.000×10^(-4)%),respectively while growth was observed on free medium(negative control).From the total of 24 treated cases of EL,14(58.3%) responded lo treatment;however,10(41.7%) did not respond to treatment.There was no significant difference in the degree of response to treatment between early and moderate cases(χ~2=0.086:P=0.408.Conclusions:It can be concluded that n-butanol extract of P.dodecandra demonstrates antifungal effects while the aqueous extract shows no antifungal activity. 关键词：EPIZOOTIC LYMPHANGITIS Phytolacca dodecandra HISTOPLASMA capsulatum var.farciminosum Minimum inhibitory CONCENTRATION Minimum fungicidal CONCENTRATION Phytochemical screening Antifungal activity Agar dilution assay Mycological culture In vivo trial Contagious DISEASE Chronic DISEASE LYMPHANGITIS

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Evaluation of berries of Phytolacca dodecandra for growth inhibition of Histoplasma capsulatum var.farciminosum and treatment of cases of epizootic lymphangitis in Ethiopia

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