Crystal structure of thermostable catechol 2,3-dioxygenase determined by multiwavelength anomalous dispersion method

查看全文 作　　者：JIANG [1]Tao;JI [1]Chaoneng;SHENG [1]Xiaoyu;CHEN [1]Mingqin;XIE [1]Yi;GONG [2]Weimin;MAO [1]Yumin 机构地区：[1]State Key Laboratory of Genetic Engineering,Institute of Genetics,School of life Sciences,Fudan University,Shanghai 200433,China;[2]Structural Biology Laboratory,School of life Sciences,University ofScience and Technology of China,Hefei 230026,China 出　　处：《Chinese Science Bulletin》索引2002年第47卷第4期,共3页 基　　金：This workwas supported by the National Natural Science Foundation of China (Grant No. 30070161); the Hundreds Talents Program of the ChineseAcademy of Sciences. 摘　　要：The selenomethionyl derivative of the thermo-stable catechol 2,3-dioxygenase (SeMet-TC23O) is expressed, purified and crystallized. By using multiwave length anoma-lous dispersion (MAD) phasing techniques, the crystal structure of TC23O at 0.3 nm resolutions is determined. TC23O is a homotetramer. Each monomer is composed of N-terminal and C-terminal domains (residues 1-153 and 153-319, respectively). The two domains are proximately symmetric by a non-crystallographic axis. Each domain contains two characteristic motifs which are found in almost all of extradial dioxygenases. 关 键 词：MULTIWAVELENGTH ANOMALOUS dispersion (MAD) X-ray diffraction THERMOSTABLE CATECHOL 2 3-dioxygenase crystal structure synchrotron light source.